Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	80,934	C→T	G342D (GGC→GAC)	leuB ←	3‑isopropylmalate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	80,934	0	C	T	98.8%	282.2 / NA	82	G342D (GGC→GAC)	leuB	3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (41/40);  minor base G (0/1);  total (41/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGC  >  NC_000913/80648‑81225                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                    tAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATc                                                                                                                                                                                                                                                                                        <  1:106712/300‑1 (MQ=255)      tCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCgg                                                                                                                                                                                                                                                                                   >  1:712025/1‑300 (MQ=255)           ggTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGa                                                                                                                                                                                                                                                                              >  2:402288/1‑300 (MQ=255)           ggTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGa                                                                                                                                                                                                                                                                              >  1:180030/1‑300 (MQ=255)              cTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACACCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATg                                                                                                                                                                                                                                                                           <  2:270950/300‑1 (MQ=255)                gggTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGcc                                                                                                                                                                                                                                                                         >  1:23561/1‑300 (MQ=255)                gggTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGcc                                                                                                                                                                                                                                                                         >  2:705888/1‑300 (MQ=255)                gggTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGACGCGTCACGGGCTAAATCCACGGTGCGAATGcc                                                                                                                                                                                                                                                                         >  2:217710/1‑300 (MQ=255)                gggTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTACAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCACATCTCATCGGTACTAACGGCGGCAGTGTCGCGGGCTAAATCCCCGGTGCGACTGcc                                                                                                                                                                                                                                                                         >  2:84168/1‑300 (MQ=255)                  gTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCAAGGGCTAAATCCCCGGTGCGAATGCctt                                                                                                                                                                                                                                                                       >  1:387122/1‑300 (MQ=255)                      agaCGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGTTCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGNTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCACTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCttcttc                                                                                                                                                                                                                                                                   <  2:171150/300‑1 (MQ=255)                           ttGTGAGCCGTGTTAGCGAAGGTTTTACCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGCGGCGGTCGATATGTAACAGTGGGGTTTCGTTTTCTGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATg                                                                                                                                                                                                                                                              <  2:137784/300‑1 (MQ=255)                                aTCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTNGTATAACGTATTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCCAACGCGCgg                                                                                                                                                                                                                                                         >  2:181188/1‑300 (MQ=255)                                aTCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCCATGATATCGCCCATTTCATCGGTACTACCGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCCAATGCGCgg                                                                                                                                                                                                                                                         >  2:107611/1‑300 (MQ=255)                                aTCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCgg                                                                                                                                                                                                                                                         >  1:82468/1‑300 (MQ=255)                                       gTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATgg                                                                                                                                                                                                                                                  <  2:23518/300‑1 (MQ=255)                                         aGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGGGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATACGCGGTTAATGgcg                                                                                                                                                                                                                                                <  1:188260/300‑1 (MQ=255)                                                   ttGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATgg                                                                                                                                                                                                                                      <  1:736845/300‑1 (MQ=255)                                                                  aCCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGcatcat                                                                                                                                                                                                                       <  2:82191/300‑1 (MQ=255)                                                                     gggCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTATGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCAAGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCgg                                                                                                                                                                                                                    >  1:304222/1‑300 (MQ=255)                                                                               gggCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACATTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACGAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCt                                                                                                                                                                                                          <  2:378418/300‑1 (MQ=255)                                                                                ggCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGNACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTg                                                                                                                                                                                                         >  1:504471/1‑300 (MQ=255)                                                                                  cgcgcAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTa                                                                                                                                                                                                       >  1:376972/1‑300 (MQ=255)                                                                                    cgcAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAAc                                                                                                                                                                                                     <  1:580430/300‑1 (MQ=255)                                                                                    cgcAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAAc                                                                                                                                                                                                     <  2:424785/300‑1 (MQ=255)                                                                                      cataCCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCANTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCNAGCCGCATCATCGGCATCCAGGCTGTAACgc                                                                                                                                                                                                   <  2:527755/297‑1 (MQ=255)                                                                                      cAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACgc                                                                                                                                                                                                   <  1:732485/300‑1 (MQ=255)                                                                                        gACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTATTAGCCATGCTTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCACATTTCATCGGTACTAACGGCGGCAGCGTCAAGGGCTAAATCCACGGTGCGAATGCCTTCTTCTCATGCGCGGTTCATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCACCCAGGATGTAACgaag                                                                                                                                                                                                 >  2:253247/1‑297 (MQ=255)                                                                                                         ggTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCATTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGaaa                                                                                                                                                                                >  2:628982/1‑300 (MQ=255)                                                                                                                   cTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGc                                                                                                                                                                      >  1:406838/1‑300 (MQ=255)                                                                                                                   cTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCATTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCAAGCGGAAGGATTTGTGc                                                                                                                                                                      >  2:96293/1‑300 (MQ=255)                                                                                                                                          atatatAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAGGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGc                                                                                                                                               >  2:636207/1‑300 (MQ=255)                                                                                                                                          atatatAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGc                                                                                                                                               >  1:335282/1‑300 (MQ=255)                                                                                                                                                     tGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCt                                                                                                                                    <  1:558714/300‑1 (MQ=255)                                                                                                                                                     tGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTCTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATNTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTANTGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCt                                                                                                                                    <  2:599990/300‑1 (MQ=255)                                                                                                                                                          tttCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGc                                                                                                                               <  1:213433/300‑1 (MQ=255)                                                                                                                                                                    ggCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGccgccc                                                                                                                     <  1:426129/300‑1 (MQ=255)                                                                                                                                                                       ttCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCACATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCAGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGAGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCcgcccgcc                                                                                                                  >  2:473665/1‑300 (MQ=255)                                                                                                                                                                                aaCGTGAGCTTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATAc                                                                                                         <  2:453727/300‑1 (MQ=255)                                                                                                                                                                                aaCGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGTGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATAc                                                                                                         <  2:668236/300‑1 (MQ=255)                                                                                                                                                                                aaCGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCGATGATATCGCTCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAATGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGTCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATAc                                                                                                         <  2:72625/300‑1 (MQ=255)                                                                                                                                                                                aaCGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATAc                                                                                                         <  1:97556/300‑1 (MQ=255)                                                                                                                                                                                aaCGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATAc                                                                                                         <  2:234063/300‑1 (MQ=255)                                                                                                                                                                                 aCGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACa                                                                                                        >  1:146571/1‑300 (MQ=255)                                                                                                                                                                                     gAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTcc                                                                                                    <  1:714120/300‑1 (MQ=255)                                                                                                                                                                                          tCGAACCATTTTGCGTATAACGTCTTAGCCATGAGTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGTGGCAGCGTCACGGGCTAAATCCCCTGTGCGAATGCCTTCTTCTAATGCGCGGTTCATGGCGCGTTCAATGGCGCAAGCCGCNTCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCNGAGCCGCCCGCCGGGGCATACAGTCCAAAAc                                                                                               <  2:516540/300‑1 (MQ=255)                                                                                                                                                                                                         tataACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGTCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCCTGCTCGTTCAGGc                                                                                <  1:402013/300‑1 (MQ=255)                                                                                                                                                                                                          ataACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTNATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCt                                                                               <  2:179145/300‑1 (MQ=255)                                                                                                                                                                                                               gTCTTAGCCATGATTACACCCCTTCTGCTGCATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTTGCGATGTTTTTGCCTGCGATACCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCCAAACCTTGCTCGTTCAGGCTGGCgg                                                                          >  2:80115/1‑300 (MQ=255)                                                                                                                                                                                                                 cTTAGTCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGGCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTTCAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGNCGCATCATCGGCATCCAGGCTGTAACNCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGaa                                                                        <  2:483957/300‑1 (MQ=255)                                                                                                                                                                                                                         tGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAt                                                                >  1:5172/1‑300 (MQ=255)                                                                                                                                                                                                                         tGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAt                                                                >  2:397227/1‑300 (MQ=255)                                                                                                                                                                                                                          gATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATc                                                               <  1:49598/300‑1 (MQ=255)                                                                                                                                                                                                                           aTTACACCCCTTCTGCTACATAGCGGGCAATGCTATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGAAAGCCGCATCATCGGCATCCAGGCTGTCACGCAGCAGCAGTGCCAGCGAAAGGGTTTGTGCAATCGGGTTGGCGATGTTTTTTCCTGCGATATCTGGTGCCGAGTCGCCCGCCGGTTCATAAAGTCCAAAAACTTGCTCGTTCAGGCTGGCGGAAGGCAACATcc                                                              >  2:77809/1‑300 (MQ=255)                                                                                                                                                                                                                           aTTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATcc                                                              >  1:38972/1‑300 (MQ=255)                                                                                                                                                                                                                            ttACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATccc                                                             <  1:171669/300‑1 (MQ=255)                                                                                                                                                                                                                               cacCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCAt                                                          >  1:24070/1‑300 (MQ=255)                                                                                                                                                                                                                                     ttCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGcc                                                    >  1:636728/1‑300 (MQ=255)                                                                                                                                                                                                                                       cTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCANGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAANCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAGAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAg                                                  >  2:590309/1‑300 (MQ=255)                                                                                                                                                                                                                                       cTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCCGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGCTATCTGGTGCCGAGCCGCCCGCCGATTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGcaag                                                  >  2:433791/1‑297 (MQ=255)                                                                                                                                                                                                                                       cTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAg                                                  >  1:723474/1‑300 (MQ=255)                                                                                                                                                                                                                                       cTGCTACATAGCGGGCAATGATATCGCCCATNTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCANGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAg                                                  >  2:535018/1‑300 (MQ=255)                                                                                                                                                                                                                                         gCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTg                                                >  2:346352/1‑300 (MQ=255)                                                                                                                                                                                                                                          cTACATAGCGTGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGGCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGa                                               <  2:364142/300‑1 (MQ=255)                                                                                                                                                                                                                                            aCATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATc                                             <  1:82958/300‑1 (MQ=255)                                                                                                                                                                                                                                              atggcaggCAATGAGATCGCCCATTTCATCGGTACTAACGTCTGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATTGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCAt                                           <  2:109623/294‑1 (MQ=255)                                                                                                                                                                                                                                              aTAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTTACGGGCTAAATCCCCGATGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCNTCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGTCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCTATTGATCAt                                           <  2:181925/300‑1 (MQ=255)                                                                                                                                                                                                                                              aTAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCATTGATCAt                                           <  2:420973/300‑1 (MQ=255)                                                                                                                                                                                                                                              aTAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCAt                                           <  1:624648/300‑1 (MQ=255)                                                                                                                                                                                                                                                    ggCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCATTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCAAGCGAAAGGATTTATGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGACTGGCGGAAGGCAACATCCACATCGAGCCAGTGGTCATTgcgcc                                     >  2:80504/1‑299 (MQ=255)                                                                                                                                                                                                                                                      cAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGTAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCACATCGAGCCAGTGATCATTGCGCACt                                   >  2:25525/1‑300 (MQ=255)                                                                                                                                                                                                                                                      cAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTNCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACt                                   >  1:538571/1‑300 (MQ=255)                                                                                                                                                                                                                                                      cAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACt                                   >  1:356834/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGTCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCNGGCTGTCACGCAGCAGCAGTGCCAGCNAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCATTCGTc                               >  2:504286/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCNGGCTGTAACGCAGCAGCAGTGCCAGCNAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTc                               >  2:498606/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTc                               <  1:119794/300‑1 (MQ=255)                                                                                                                                                                                                                                                               cGCCCATTTCATCGGTACTAACGGCGGCAGTGTCACGGGCTAAACCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTACTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTcagaca                          <  2:74868/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        cATCGGTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAACGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGNTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCNGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGc                 <  2:591777/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            ggTACTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCACAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGACCATTGCGAACTCGTCAGACAGAACGTAGCCaaa             >  2:103821/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                cTAACGGCGGCAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTACAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAgg         <  1:420001/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gcggcANCGTCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGNTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAg   <  2:617321/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       cggcAGCGTCACGGGCTAAATCCCCGGTGCGAATGCCGTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGGGCCAGCGAAAGGATTTGGGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCATTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGc  <  1:389253/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                    TAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGC  >  NC_000913/80648‑81225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑