Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,225,414	G→A	V928I (GTT→ATT)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,225,414	0	G	A	100.0%	229.6 / NA	63	V928I (GTT→ATT)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/31);  total (32/31)

GGCCAGGTTAACGTGGCGCTTTCCGGCGAGCGTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGGTTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTACCGCGCAAAGCCTGGCGTCGTATGAGTTCGGCGCAGGGTTCTTTTCCACGAATTTGCAC  >  NC_000913/3225126‑3225707                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                      ggCCAGGTTAACGTGGCGCTTTCCGGCGAGCGTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAgcgc                                                                                                                                                                                                                                                                                            >  1:562408/1‑300 (MQ=255)           aCGTGGCGCTTTCCGGCGAGCGTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACt                                                                                                                                                                                                                                                                                  <  1:585201/300‑1 (MQ=255)                       ccGGCGAGCGTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTCTCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTCACCGACACGGTAACAGTCTGCTGGTGATTCCTCAGCGCCAAACTAACCTCAGCGCCTGGc                                                                                                                                                                                                                                                                      >  2:135015/1‑300 (MQ=255)                            gAGCGTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTNCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACtat                                                                                                                                                                                                                                                                 <  1:477016/300‑1 (MQ=255)                              gCGTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACTGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATAc                                                                                                                                                                                                                                                               <  1:206007/300‑1 (MQ=255)                              gCGTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATAc                                                                                                                                                                                                                                                               <  2:415895/300‑1 (MQ=255)                                gctACGGCTACTCTCCGCACATCACTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGTTGGCGTATTACGGTCGTGGACCGGTCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATAccc                                                                                                                                                                                                                                                             <  2:39101/298‑1 (MQ=255)                                gTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATAccc                                                                                                                                                                                                                                                             <  1:716688/300‑1 (MQ=255)                                     ggCGACTATCCGCACATCATTCTATGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGTTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCATTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAGTTAACTTCAGCGCCTGGCACTATACCCAGGaa                                                                                                                                                                                                                                                        <  2:602857/300‑1 (MQ=255)                                      gCGACTNTCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGNATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGaaa                                                                                                                                                                                                                                                       <  2:586173/300‑1 (MQ=255)                                                gCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGc                                                                                                                                                                                                                                             <  1:663197/300‑1 (MQ=255)                                                   catcatTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGc                                                                                                                                                                                                                                          >  2:541231/1‑300 (MQ=255)                                                          ccGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCAc                                                                                                                                                                                                                                   <  1:162073/300‑1 (MQ=255)                                                            gTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTg                                                                                                                                                                                                                                 >  1:607003/1‑300 (MQ=255)                                                               cATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTTGCGTATTACGGNCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATNAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTaa                                                                                                                                                                                                                              <  2:670150/300‑1 (MQ=255)                                                               cATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTaa                                                                                                                                                                                                                              <  1:364691/300‑1 (MQ=255)                                                                 tCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACGATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACg                                                                                                                                                                                                                            <  1:425383/300‑1 (MQ=255)                                                                   ggTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAg                                                                                                                                                                                                                          >  1:303475/1‑300 (MQ=255)                                                                                 aaTTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCAACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTCACCTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCCGCCCAGCACTGTAACGAGCTGcagtgcagtgg                                                                                                                                                                                                            >  2:319541/1‑299 (MQ=255)                                                                                   ttAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGtgatg                                                                                                                                                                                                          >  1:357682/1‑300 (MQ=255)                                                                                      aCGGCGAATACGATCAGTTGTCGGATTACGGTCGTGGACCGGGCGAAAACGACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCTCAAAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACa                                                                                                                                                                                                       <  2:601571/300‑1 (MQ=255)                                                                                      aCGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGNCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACa                                                                                                                                                                                                       <  2:531913/300‑1 (MQ=255)                                                                                        ggCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATc                                                                                                                                                                                                     <  1:180591/300‑1 (MQ=255)                                                                                           gAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTACCGCAGAAAAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCAca                                                                                                                                                                                                  >  2:347816/1‑299 (MQ=255)                                                                                            aaTACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGTAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCAccc                                                                                                                                                                                                 <  2:597844/300‑1 (MQ=255)                                                                                                cGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACCTCACCCTGaa                                                                                                                                                                                             >  2:320911/1‑300 (MQ=255)                                                                                                          gCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCAc                                                                                                                                                                                   >  1:582626/1‑300 (MQ=255)                                                                                                            gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGNGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCa                                                                                                                                                                                 >  1:493478/1‑300 (MQ=255)                                                                                                            gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCa                                                                                                                                                                                 >  1:589950/1‑300 (MQ=255)                                                                                                             tATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGACCTCTCCCTTCCCGAAGAACAACGGTAACCGTCAGCCTGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATAACCAGGAAAACATCCACGCTGCCCAGCACTGTAACAAACTGCAGCGCCGTGATGACATAACCCTGACCCCCGCTCACCAg                                                                                                                                                                                >  2:5345/1‑300 (MQ=255)                                                                                                                    gTCGTGGACTGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCNGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTNACGAGCTGCAGCGCAGTGATGACATCAACCTGAACCTCGATCACCAGCTGCTTg                                                                                                                                                                         >  2:654416/1‑300 (MQ=255)                                                                                                                    gTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCTGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACNTCAGCGCATGGCACTATACCCAGGAAAACATCCACACTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCAACATGAACCTCGATCCCCAGCTGCTTg                                                                                                                                                                         >  2:627462/1‑300 (MQ=255)                                                                                                                       gTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGcc                                                                                                                                                                      <  1:554161/300‑1 (MQ=255)                                                                                                                                       cTACGCCGACAGCCAGCAGGCTAACCTCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCAACCTGAACCTCGATCACCAGCTGCTTGGCATCGGCTCCAACTACTg                                                                                                                                                      >  2:285354/1‑300 (MQ=255)                                                                                                                                                cagccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTNGAGAACTATCCCTTCCCGCAGAACAACCGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGa                                                                                                                                             <  2:536208/300‑1 (MQ=255)                                                                                                                                                cagccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGa                                                                                                                                             <  2:105148/300‑1 (MQ=255)                                                                                                                                                    cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTg                                                                                                                                         >  2:547058/1‑300 (MQ=255)                                                                                                                                                         ggCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCCGCACTGTAACGAGTTGCAGCGCAGTGATTACATCAACCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGa                                                                                                                                    >  1:586939/1‑300 (MQ=255)                                                                                                                                                         ggCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGa                                                                                                                                    >  1:317618/1‑300 (MQ=255)                                                                                                                                                         ggCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCAGCAGAGCAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGTTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGa                                                                                                                                    >  2:307388/1‑300 (MQ=255)                                                                                                                                                          gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCCGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGAc                                                                                                                                   <  2:614635/300‑1 (MQ=255)                                                                                                                                                          gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGAc                                                                                                                                   >  1:97650/1‑300 (MQ=255)                                                                                                                                                          gCTAACAGCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGAc                                                                                                                                   <  2:283288/300‑1 (MQ=255)                                                                                                                                                                  catcAATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGgcg                                                                                                                           <  2:251655/300‑1 (MQ=255)                                                                                                                                                                   atcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAAAACTATCCCTTCCCGCAGGACAACGTTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAGCGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAGCGAGCTGCAGCGCAGTGATGACATTACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGgcgc                                                                                                                          <  2:20661/300‑1 (MQ=255)                                                                                                                                                                         atCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTgg                                                                                                                    >  1:667308/1‑300 (MQ=255)                                                                                                                                                                            tGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTc                                                                                                                 <  1:651892/300‑1 (MQ=255)                                                                                                                                                                            tGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTc                                                                                                                 <  2:637600/300‑1 (MQ=255)                                                                                                                                                                              gcgcAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCg                                                                                                               >  1:19159/1‑300 (MQ=255)                                                                                                                                                                                     aCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTc                                                                                                        >  1:391367/1‑300 (MQ=255)                                                                                                                                                                                        gTCGATGCCATGTTCGAGAACTATCCCTTACCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGc                                                                                                     >  2:294954/1‑300 (MQ=255)                                                                                                                                                                                                                 ccTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGttt                                                                            >  1:651729/1‑300 (MQ=255)                                                                                                                                                                                                                      ccGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGc                                                                       >  1:423468/1‑300 (MQ=255)                                                                                                                                                                                                                                acGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTa                                                             >  1:416676/1‑300 (MQ=255)                                                                                                                                                                                                                                acGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCATGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTa                                                             >  1:416672/1‑300 (MQ=255)                                                                                                                                                                                                                                 cGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTAc                                                            >  1:602753/1‑300 (MQ=255)                                                                                                                                                                                                                                                  tCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTACCGCGCAAAGCCTGGcgt                                           <  1:556021/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aCGGCACCGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGTCGTAGAAGCTACCGCGCAAAGCCTGGCGTCGTATGAg                                   <  2:148874/300‑1 (MQ=255)                                                                                                                                                                                                                                                            ggCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTACCGCGCAAAGCCTGGCGTCGTATGAGtt                                 >  1:50440/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTACCGCGCAAAGCCTGGCGTCGTATGAGTTc                                >  1:176791/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            cACGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCANTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTACCGCGCAAAGCCTGGCGTCGTATGAGTTCGGCGCAGGGTTCttt                 <  1:483398/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              cGGTAACGGTCTGCTGGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTACCGCGCAAAGCCTGGCGTCGTATGAGTTCGGCGCAGGGTTCTTTTc               <  1:77220/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           ctgGTGATTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTACCGCGCAAAGCCTGGCGTCGTATGAGTTCGGCGCAGGGTTCTTTTCCACGAATTTGcac  <  2:368977/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                      GGCCAGGTTAACGTGGCGCTTTCCGGCGAGCGTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACGGTAACGGTCTGCTGGTGGTTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTATACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGAAGCTACCGCGCAAAGCCTGGCGTCGTATGAGTTCGGCGCAGGGTTCTTTTCCACGAATTTGCAC  >  NC_000913/3225126‑3225707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑