BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,269,958	C→T	intergenic (+391/‑21)	lpxT → / → mepS	lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,269,958	0	C	T	100.0%	401.6 / NA	113	intergenic (+391/‑21)	lpxT/mepS	lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (73/40); total (73/40)

Only 100 of 113 total aligned reads displayed.

TCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAG  >  NC_000913/2269663‑2270249

gttattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCa                                                                                                                                                                                                                                                                                                 <  1:159085‑M1/87‑1 (MQ=255)

  tattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagaganctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTANTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAg                                                                                                                                                                                                                                                                                               <  2:667693‑M1/89‑1 (MQ=255)

  tattcacgctggccaacctgtttcgggcggaccaaatgagacgtcagtgggagagatctcactaaaaactgtggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAg                                                                                                                                                                                                                                                                                               >  2:691866‑M1/212‑300 (MQ=255)

    ttcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAggg                                                                                                                                                                                                                                                                                             >  1:408692‑M1/210‑300 (MQ=255)

            ggccaacctgtttcgggcggaccaaatgatangtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagnaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAaaacaaa                                                                                                                                                                                                                                                                                     <  2:497505‑M1/99‑1 (MQ=255)

            ggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAaaacaaa                                                                                                                                                                                                                                                                                     <  1:76018‑M1/99‑1 (MQ=255)

            ggccaacctgtttcgggcggaccaaatcatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcaCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAaaacaaa                                                                                                                                                                                                                                                                                     <  1:139630‑M1/99‑1 (MQ=255)

                aacctgtttcgggcggaccaaatgatacgtcagtgggggagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAaacaac                                                                                                                                                                                                                                                                                 >  1:408890‑M1/198‑300 (MQ=255)

                aacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAaacaac                                                                                                                                                                                                                                                                                 <  2:671472‑M1/103‑1 (MQ=255)

                     gtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcgnctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcancttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGt                                                                                                                                                                                                                                                                            <  2:561486‑M1/108‑1 (MQ=255)

                     gtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCCGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGt                                                                                                                                                                                                                                                                            <  2:546355‑M1/108‑1 (MQ=255)

                     gtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGt                                                                                                                                                                                                                                                                            <  1:281222‑M1/108‑1 (MQ=255)

                     gtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGt                                                                                                                                                                                                                                                                            >  2:210778‑M1/193‑300 (MQ=255)

                     gtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactgggganaacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATNTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGt                                                                                                                                                                                                                                                                            <  2:668724‑M1/108‑1 (MQ=255)

                     gtttcggacggaccaaatgatacgtcagtgggagagatctaactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgaatgagtcagccgagaagaatttcccagcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTATAGTGTAGCTTGGCATTTTTTTATAACGATACTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACGTGGt                                                                                                                                                                                                                                                                            <  2:196031‑M1/108‑1 (MQ=255)

                           ggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAAtc                                                                                                                                                                                                                                                                      >  1:243347‑M1/187‑300 (MQ=255)

                           ggcggaccaaatgatacggcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAATGGAAAACAAACAACATGGTCAAAtc                                                                                                                                                                                                                                                                      >  1:604130‑M1/187‑300 (MQ=255)

                            gcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAAtct                                                                                                                                                                                                                                                                     >  1:597241‑M1/186‑300 (MQ=255)

                            gcggaccaaatgatacgccagtgggagagatctcactaaaaacttgggataacgccttaaatggcgaagaaacggtctaaataggctgatgcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAAtct                                                                                                                                                                                                                                                                     >  2:31974‑M1/186‑300 (MQ=255)

                               gaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttcaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcacacatgaagaaatgaaatgactgagtcggccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTAAACATGCCCAGGTAATTAGTCTAGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAACCAAAcaacatggtccactctcca                                                                                                                                                                                                                                                                  <  2:139380‑M1/118‑10 (MQ=255)

                               gaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttanatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTAGTGTCGCTTGGCATTTTTTTATAANGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCaa                                                                                                                                                                                                                                                                  <  2:665465‑M1/118‑1 (MQ=255)

                                accaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTcaaa                                                                                                                                                                                                                                                                 <  2:550688‑M1/119‑2 (MQ=255)

                                            gtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttacCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGATTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAATCGATTTTGAGat                                                                                                                                                                                                                                                     <  1:46776‑M1/131‑1 (MQ=255)

                                                gtgggagagatctcactaaaaactggggatancgccttcaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactnagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGCTATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGatatat                                                                                                                                                                                                                                                 <  2:505617‑M1/135‑1 (MQ=255)

                                                  ggtagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagnaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCt                                                                                                                                                                                                                                               >  1:493659‑M1/164‑300 (MQ=255)

                                                  gggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCt                                                                                                                                                                                                                                               >  1:107404‑M1/164‑300 (MQ=255)

                                                   ggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCtt                                                                                                                                                                                                                                              <  1:610845‑M1/138‑1 (MQ=255)

                                                       agatctgactaaaaactggggataacgccttaaatggcgacgaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagcctagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTTAGATATATCTTgcgc                                                                                                                                                                                                                                          >  2:611195‑M1/159‑300 (MQ=255)

                                                                   aaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCcgcg                                                                                                                                                                                                                              >  1:690198‑M1/147‑300 (MQ=255)

                                                                      ctggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGAtt                                                                                                                                                                                                                           >  1:716278‑M1/144‑300 (MQ=255)

                                                                                cgccttgaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGNCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCAATTTTGAGATATATNTTGCGCGGGATTCCCGCGATTGCAGTAGCgg                                                                                                                                                                                                                 >  2:496535‑M1/134‑300 (MQ=255)

                                                                                cgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCgg                                                                                                                                                                                                                 >  1:698812‑M1/134‑300 (MQ=255)

                                                                                cgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaacttccccgcttattcgcaccttccCTGAAGGAATTGTTGCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAACCTCAACCAATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCgg                                                                                                                                                                                                                 >  1:116279‑M1/134‑300 (MQ=255)

                                                                                               agaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCAt                                                                                                                                                                                                  >  1:550840‑M1/119‑300 (MQ=255)

                                                                                                gaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTNAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATg                                                                                                                                                                                                 <  2:626435‑M1/183‑1 (MQ=255)

                                                                                                 aaacggtctaaatatgctgattcaatgcatttacggtagaaaaaatcggctcaaacattaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAATGAATTGTTTCNACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTTAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGt                                                                                                                                                                                                >  2:168925‑M1/117‑300 (MQ=255)

                                                                                                 aaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGt                                                                                                                                                                                                >  1:437465‑M1/117‑300 (MQ=255)

                                                                                                      gtctaaagatgctgattcaaggcatttacggtagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGc                                                                                                                                                                                           >  2:13238‑M1/112‑300 (MQ=255)

                                                                                                        ctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGGAGCGGTTCTGCTTTCTGCATGTAGTGCaa                                                                                                                                                                                         >  1:43577‑M1/110‑300 (MQ=255)

                                                                                                          aaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcactttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCACATCTCAACCGATTTTGGGATCTATCTTGCGCGGGATTCCCGCGCTTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAAt                                                                                                                                                                                       <  2:50047‑M1/193‑1 (MQ=255)

                                                                                                           aataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagcctagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATa                                                                                                                                                                                      >  2:402567‑M1/107‑300 (MQ=255)

                                                                                                           aataggctgatgcaaggcatttactggagaaaaaatcggctcaaacattaagaaatgaaacgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGGTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATa                                                                                                                                                                                      >  2:402576‑M1/107‑300 (MQ=255)

                                                                                                               ggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGNATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAacac                                                                                                                                                                                  >  1:494727‑M1/103‑300 (MQ=255)

                                                                                                                    attcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCaa                                                                                                                                                                             <  1:143043‑M1/203‑1 (MQ=255)

                                                                                                                         aggcgtttacgggagaaaaaatcggctcaaacctgaagaaataaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttacATTAAGGAATTATTTCAACATGCCCAGGTAATTAGTCTAGTGTCGCCTGGCATTTTTTTATAACGATATTTGTCGTTACGGATTTCAAGGGGAGACAAACAACATGGTCAAATCTAAAACGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAat                                                                                                                                                                        <  2:333812‑M1/208‑1 (MQ=255)

                                                                                                                         aggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGGTTTTGAGATATATCTTGCGCGGGATTCCCGCGCTTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAat                                                                                                                                                                        <  2:139019‑M1/208‑1 (MQ=255)

                                                                                                                                        aaaaaatcggctcaaacattaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTTAGaca                                                                                                                                                         >  1:375682‑M1/78‑300 (MQ=255)

                                                                                                                                        aaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGaca                                                                                                                                                         >  1:445096‑M1/78‑300 (MQ=255)

                                                                                                                                             atcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCGTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAATGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGc                                                                                                                                                    >  1:689353‑M1/73‑300 (MQ=255)

                                                                                                                                               cggctcaaacatgaagaaatgaaatgattgggtcagtcgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTANGGATTTCAAGGGAAAGCAAACAACATNGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACGCGTGCAg                                                                                                                                                  >  2:478581‑M1/71‑300 (MQ=255)

                                                                                                                                               cggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatctccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTTAGACACGTGCAg                                                                                                                                                  >  1:621111‑M1/71‑300 (MQ=255)

                                                                                                                                                  ctcaaacatgaagaaatgaaatgactgagtcagcctagaagaatttccccgcttattcgcaccttccCTTAATGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTTAGACACGTGCAGTgg                                                                                                                                               >  2:425743‑M1/68‑300 (MQ=255)

                                                                                                                                                  ctcaaacatgaagaaatgaaatgactgagtcagcctagaagaatttccccgcttattcgcaccttccCTTAATGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTgg                                                                                                                                               >  1:276339‑M1/68‑300 (MQ=255)

                                                                                                                                                  ctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTgg                                                                                                                                               >  1:276317‑M1/68‑300 (MQ=255)

                                                                                                                                                  ctcaaacatgaagaaaggaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATTCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTGAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGAGATATCTTGCGCGGGATGCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTTAGACACGTGCAGTgg                                                                                                                                               >  1:283722‑M1/68‑300 (MQ=255)

                                                                                                                                                    caaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAaataacaccgcaaagaatatgcactgtctcttatacacatctcgc                                                                                                                                             >  1:541129‑M1/66‑278 (MQ=255)

                                                                                                                                                       acatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGt                                                                                                                                          >  1:423077‑M1/63‑300 (MQ=255)

                                                                                                                                                         atgaagaaattaactgactgagtcagcctagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTTTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTTAGACACGTGCAGTGGGTAGTGa                                                                                                                                        >  2:242158‑M1/61‑300 (MQ=255)

                                                                                                                                                         atgaagaaatgaaattattgagtcagccgagaagaatctccccgcttattcgcaccttccCTTAAGTAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGa                                                                                                                                        >  2:229703‑M1/61‑300 (MQ=255)

                                                                                                                                                         atgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGa                                                                                                                                        >  1:339091‑M1/61‑300 (MQ=255)

                                                                                                                                                         atgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCGTAAGGAATTGTGTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATNTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTNAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGa                                                                                                                                        >  2:566803‑M1/61‑300 (MQ=255)

                                                                                                                                                          tgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGaa                                                                                                                                       >  1:533058‑M1/60‑300 (MQ=255)

                                                                                                                                                              gaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAAcat                                                                                                                                   >  1:82554‑M1/56‑300 (MQ=255)

                                                                                                                                                              gaaatgaaatgactgagtcagccgagaagaatttccccgcctattcgcaccttccCTTAAGGAGTTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAAcat                                                                                                                                   >  1:314938‑M1/56‑300 (MQ=255)

                                                                                                                                                                aatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAAcatca                                                                                                                                 <  1:384337‑M1/247‑1 (MQ=255)

                                                                                                                                                                    aaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCAc                                                                                                                             >  1:686910‑M1/50‑300 (MQ=255)

                                                                                                                                                                         actgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCaa                                                                                                                        >  1:400135‑M1/45‑300 (MQ=255)

                                                                                                                                                                           tgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGc                                                                                                                      >  1:122933‑M1/43‑300 (MQ=255)

                                                                                                                                                                            gagtcagccgagaggaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAACTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTACTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCt                                                                                                                     >  2:104836‑M1/42‑300 (MQ=255)

                                                                                                                                                                            gagtcagccgagaagaatttccccgcttgttcgcaccttccCTTAAGGAATTGTTTCAACAGGCCCGGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCt                                                                                                                     >  1:154299‑M1/42‑300 (MQ=255)

                                                                                                                                                                            gagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCt                                                                                                                     >  1:153927‑M1/42‑300 (MQ=255)

                                                                                                                                                                             agtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAGCACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCtt                                                                                                                    <  2:251049‑M1/260‑1 (MQ=255)

                                                                                                                                                                                                     cttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCNCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTAAGGATGAATTTGAAAACCTgg                                                                                            <  2:175271‑M1/284‑1 (MQ=255)

                                                                                                                                                                                                            acaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTaa                                                                                     >  2:115738‑M1/10‑300 (MQ=255)

                                                                                                                                                                                                                  tccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTAAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGa                                                                               <  1:4632‑M1/297‑1 (MQ=255)

                                                                                                                                                                                                                                          gCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCa                                                       >  1:439456/1‑300 (MQ=255)

                                                                                                                                                                                                                                             cAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGNATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTAAGGATGNATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGAGAATTATGGATCAGTa                                                    >  2:669620/1‑300 (MQ=255)

                                                                                                                                                                                                                                              aGGTAATTGGTCTCTTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAATGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTTAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTAt                                                   <  1:531240/300‑1 (MQ=255)

                                                                                                                                                                                                                                               ggTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAGAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATg                                                  >  2:393345/1‑300 (MQ=255)

                                                                                                                                                                                                                                               ggTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGCTTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAACCGCGAATTATGGATCAGCATg                                                  >  2:641810/1‑300 (MQ=255)

                                                                                                                                                                                                                                               ggTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATg                                                  >  1:475339/1‑300 (MQ=255)

                                                                                                                                                                                                                                               ggTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTAAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATg                                                  >  2:635536/1‑300 (MQ=255)

                                                                                                                                                                                                                                                gTAATTATTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGc                                                 <  1:683880/300‑1 (MQ=255)

                                                                                                                                                                                                                                                gTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTTAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGc                                                 <  1:326533/300‑1 (MQ=255)

                                                                                                                                                                                                                                                  aaTTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTAAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTg                                               >  1:629953/1‑300 (MQ=255)

                                                                                                                                                                                                                                                  aaTTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAATGCAAATAACAACGCAAAGAATATGTATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCAATGCAAGTTTTTAAGGATGAATTTGAAAACCTGGTTCGTAGTGTCGACGTGAAATCGCGGATTATGGATCAGTATGCTg                                               >  2:330844/1‑300 (MQ=255)

                                                                                                                                                                                                                                                   aTTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGNGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGcgga                                              <  1:513849/300‑4 (MQ=255)

                                                                                                                                                                                                                                                      aGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTAAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTg                                           >  1:347769/1‑300 (MQ=255)

                                                                                                                                                                                                                                                       gTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTgg                                          >  1:455875/1‑300 (MQ=255)

                                                                                                                                                                                                                                                          tcGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGaaa                                       <  1:582734/300‑1 (MQ=255)

                                                                                                                                                                                                                                                          tcGTGTCGCTTGGCATTTTTTTAGAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGaaa                                       <  1:680031/300‑1 (MQ=255)

                                                                                                                                                                                                                                                           cGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTNGCGGTTCTGCTTTCTGCATGTAGTGCNAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAg                                      >  2:469106/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                   ttGGCATTTTTTTATAACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATANCACCGCAAAGAATATGCATCCTGAGANACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGt                              <  2:483023/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                ataACGATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGggcg                 <  1:552802/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                     gATATTTGTCGTTAAGGATTTCAAGGGAAAANAAACAACATGGTCAAATCTAAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGAGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGNAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTAAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGgcagc            >  2:504150/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                     gATATTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGgcagc            <  1:591979/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                      atatTTGTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTAAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGTTGACTGGAAAGGCGTACGTTATCGTCTGGGCGgcagca           >  2:202105/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                            gTCGTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTaaaa     >  1:398766/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                               gTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAGTCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAg  <  2:75825/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                               gTTAAGGATTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTAAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGTGGCAGCAATAAAAAAg  >  2:142261/1‑300 (MQ=255)

TCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAG  >  NC_000913/2269663‑2270249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`