Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,452	0	C	A	30.9%	77.1 / 13.7	56	intergenic (‑196/‑11)	ttdR/ttdA	DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (11/27);  new base A (17/0);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 4.38e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTC  >  NC_000913/3206204‑3206699                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                              aCGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTg                                                                                                                                                                                                                                                             <  1:433087/251‑1 (MQ=255)  cGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCGTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCGCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CTGTG‑AATTTTT‑CCAGGCAAATACAAAAACTgg                                                                                                                                                                                                                                                            <  2:655260/251‑1 (MQ=255)          aCCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTAATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑cc                                                                                                                                                                                                                                                   <  2:197805/250‑1 (MQ=255)           ccAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGGGTAATTTTT‑CC‑GACAAAAAAAAAAAATGGAGGTTG‑cc                                                                                                                                                                                                                                                   >  2:87975/1‑250 (MQ=255)                aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑TCACACAAATACAAAAACTTGAG‑TTG‑CCatgatg                                                                                                                                                                                                                                             >  1:641957/1‑250 (MQ=255)                       aGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAg                                                                                                                                                                                                                                     <  1:419410/251‑1 (MQ=255)                        gTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGt                                                                                                                                                                                                                                    <  1:670839/251‑1 (MQ=255)                            ttGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑GATATTT‑TCAGAAAAAAAAAAAAAATGGGG‑GTG‑CCatgggagggggaagaaat                                                                                                                                                                                                                                 >  2:458099/1‑235 (MQ=255)                                   aaGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑cc                                                                                                                                                                                                                                                   <  2:17711/225‑1 (MQ=255)                                                                ccGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCg                                                                                                                                                                                         <  1:127530/251‑1 (MQ=255)                                                                 cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑GATTTTT‑TCAGACAAATAAAAAAAATGGAG‑GTG‑GCATTATGAGCGAAAGGAAAAA‑GCAAAAAGGAGGGAA‑TAAGGTG‑Accaagaatgtcgg                                                                                                                                                                                        >  1:666975/1‑239 (MQ=255)                                                                          gATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTa                                                                                                                                                                                <  1:207806/250‑1 (MQ=255)                                                                           aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑TCAGGCAAAAAAAAAAAATGGAG‑TTG‑CCATGATGAGCGGAAGAAAAAA‑ACAAAAAGCAGTGAA‑TAAGTTG‑AAAGAGATTGTCGCTAACTTTaac                                                                                                                                                                              >  1:191587/1‑249 (MQ=255)                                                                                        aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCCGACAAATACAAAAAATGGAG‑TTG‑GCATGATGAGGGGAAGGAAAAA‑GCAACAGGCAGGGAA‑TAAGTTG‑AAAGAGATTTTCGCTAACTTTTCCCCCATGATTTCtt                                                                                                                                                                 >  1:468477/1‑250 (MQ=255)                                                                                                     ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑TCCGACAAAAAAAAAAAATGGGG‑GTG‑CCATGATGGGGGAAAGTAATAA‑GCAACAGGGAGTGAA‑AAAGTTG‑ACAGAGAGTGTCGCTAACTTTTCCCCCCTGATTTTTAccccgaagcctgg                                                                                                                                                    >  1:210955/1‑241 (MQ=255)                                                                                                      tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑GATTTTT‑TCCGACAAAAACAAAAAATGGAG‑TTG‑GCCTGATGAGCGGAAGAAAAAA‑ACAACAGGCAGTGAA‑TAAATTGGACA‑AGATTTGCGGCAAATTTACCGCCCTGATTTTTAccccaaagcccggt                                                                                                                                                   >  1:634344/1‑240 (MQ=255)                                                                                                                 gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑TCAGACAAATACAAAAAATGGAG‑GTG‑CCATGATGAGCGAAAGGAAAAA‑AAAACAAGCCGTGAA‑TAAAATG‑ACAGAGATTGTCGCCAAATTTACCCCCCTGATTTTTACCCCCAAGCCTGATGACgcgggggg                                                                                                                                        >  1:404075/1‑244 (MQ=255)                                                                                                                    ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AAATTTT‑TCAAAAAAAAACAAAAACCTGAG‑TTG‑CCCTGGTGAGAGAAAAGAATAA‑ACAACAAGCAGAGAA‑AAAATTG‑ACAAAGATTTTCGCTAACGTTACAGCCACCTATTCTACCCGAATGCCCGATGAccggggggaga                                                                                                                                      >  2:223879/1‑240 (MQ=255)                                                                                                                    cgggCGAAATATTCATTCTTATTTCCGCGTTGATTCACGAATTGTTAATTACATTTTCAGGGTGGAAAAAAATAAAAAACCAAATTTTGAGTGGTAGTTTGGGG‑GGGTG‑AATTTTT‑CAAGAAAAATAAAAAAACTGGAG‑TTG‑CCAGGATGAGCAAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                                                                                                                                       <  2:641957/247‑1 (MQ=255)                                                                                                                       gcgcAATATTCATTCTTATTTTCGGGTTGATCCACAAACTGTTAATTAAATATTCAGGGTGGAAAAAAATAAAAAACCACATTTTAAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATAAAAAAACGGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAGGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGATGTGGTGGATAAACt                                                                                                                                  <  1:62517/251‑1 (MQ=255)                                                                                                                         gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATACCTGATGACGTGGTGGATAAACTaa                                                                                                                                <  1:12517/251‑1 (MQ=255)                                                                                                                                            tCGCGTTAATTCACGAACTTTTAATTAAATTTTCAGGGTGGAAAAAAATAAAAAACCACATTTTTAGGGGTAGTTTGGGG‑CGGTG‑AATTTTT‑CAAAACAAAAAAAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAAAAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCg                                                                                                              <  2:404075/250‑1 (MQ=255)                                                                                                                                                gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AAATTTT‑TTAAACAAAAAAAAAAAAAGGAG‑TTGGCCA‑GATGAGCGAAAATAATAA‑ACAAAAAGCCGTGAA‑AAAGTTG‑ACAGAGAATGGTGCTCACTTTATCGCCACGCTTTTTACCCGAATGCCTGATGACGGGGTGGATAAAATAAAAAAGATAAAGGATGCCGaaaa                                                                                                          >  2:209263/1‑249 (MQ=255)                                                                                                                                                     ttCACGAACGTTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAACCTGGAG‑TTG‑CCATGATGAGCGAAATTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGGCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcgt                                                                                                     <  1:663496/250‑1 (MQ=255)                                                                                                                                                      tCACGAACTGTTAATTACATATCCACGGGGAAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTA‑AATTTTT‑CCAGACAAATACAAAAACGGGAT‑TTC‑CCATGAGGATCAAAAGTAATAG‑GCAACAGGCAGTGAA‑TATGTTG‑ACATAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACgtcgtc                                                                                                    <  1:266322/250‑1 (MQ=255)                                                                                                                                                       cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑TCCGACAAATACAAAAAATGGAG‑GTG‑CCATGATGAGCGGAAATAAAAA‑ACAACAAGCAGGGAA‑AAAGTTG‑ACAGAGAATGTCGCTAAATTTACCGCCATGATTTTTACCCGAAAGCCTGATGACGGGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGAAACGtcgtcgg                                                                                                  >  1:56069/1‑250 (MQ=255)                                                                                                                                                        ccGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGG‑CGGTG‑AATTTTT‑CCAGAAAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCAAAAGAAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAt                                                                                                 <  1:515193/250‑1 (MQ=255)                                                                                                                                                        aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑TCCGACAAATACAAAAAATGGAG‑GTG‑CCATGATGAGCGGAAATAATAA‑GCAAAAGGCAGTGAA‑TAAATTG‑TCAGAGATTTTCGCTAACTTTACCGCCCTGATTTCTTCCCCAATTCCTGGTGACGTGGTGGGTAAAATAAAAAAACTAAAAGATGCCGCAAAGTCGTCGa                                                                                                  >  1:286117/1‑250 (MQ=255)                                                                                                                                                           aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATggg                                                                                              <  1:87975/251‑1 (MQ=255)                                                                                                                                                                taaaTTACAATTTCACGGTGGAAAAAAATATAAAACCCCATTTTTAGGGGTAGTTTGTGG‑GGGTA‑AATTTTT‑CCAGAAAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑AAAGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑aaa                                                                                         <  1:380846/248‑1 (MQ=255)                                                                                                                                                                     tataggttCACGGGGGCAAAAAATAAAACACCTGATTTTTAGTGGTAGTTTGTGG‑GGGTG‑AATTTTT‑CCAAACAAATACAAAATCGTGAG‑TTG‑CCTTTTTGTGCGAAGGTAATAA‑GCAACAGGAAGTGAA‑TAGGTTG‑ACAGTGATGGTCGCAATCTGGACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTAt                                                                                    <  2:224165/244‑1 (MQ=255)                                                                                                                                                                        atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑TCAAACAAATAAAAAAAATGGAG‑TTG‑GCATGATGAGCGAAAGTAATAA‑ACAAAAAGCAGTGAA‑TAAGTTG‑ACAGAGAATGTCGCTAAATTTAACGCCATGATTTTTATCCCAATGCCTGATGACGCGGGGGGGAAACTAAAACAGCTAAAAGAAGCCGAAACAGCGGCGATGGGG‑AAAAATATTTAc                                                                                >  1:450752/1‑251 (MQ=255)                                                                                                                                                                             cACGGTGGAAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGGGG‑CGGTG‑AATTTTC‑CAAAAAAAATAAAAAAACTGGAG‑TTG‑CCATGAGGAGCGAAAGTAATAA‑GCAACAGGCAGTAAA‑TAAGTTG‑ACAGAGATTTTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATAc                                                                           <  1:377765/251‑1 (MQ=255)                                                                                                                                                                                gggggaaaaaaaaaaaaaacaccaTTTTAAGTGGTATTTTGGGG‑GGGTG‑AATTTTT‑ACAAAAAAATAAAAAAATGGGAT‑GGG‑CAATGATGAGAGAAAGAAAAAA‑GAAAAAGGCAGTAAT‑TAAGTTG‑AAAGAGATTGGCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGAt                                                                        <  2:274668/229‑1 (MQ=255)                                                                                                                                                                                ggTGGCAAAAAATAGAAACCCACATTTTTAGTGGTAGTTTGTGG‑CGGTA‑AGTTTTT‑CAAGACAAATAAAAAACCTGGAG‑TTG‑CAATGAGGGGCAAAAGTAATAA‑GCAAGAGGCAGTGAA‑‑AAATTGCACAGAGGTTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGAt                                                                        <  2:332429/251‑1 (MQ=255)                                                                                                                                                                                      aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑TCAGACAAAAACAAAAAATGGAG‑GTG‑CCATGATGAGCGGAAGAAATAA‑GCAACAGGCAGTGAA‑TAAATTG‑ACAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTACCCCAAAGCCTGATGACGGGGGGGATAAAATAAAAAAGCTAAAGGATGCCGAAACGGCGTCGGTGGGG‑GAAATTATCTAACATACGATGtttgg                                                                  >  1:120690/1‑248 (MQ=255)                                                                                                                                                                                      aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGGG‑AAATTTT‑TCAAACAAAAAAAAAAAATGGAG‑TTT‑GCATGATGGGCGAAAGGAATAA‑ACAAAAAGCCGTGAA‑AAAAATG‑ACAGAGAATGTCGCTAACTTTACCCCCCCGATTTCTACCCCAATGCCCGATGAAGGGGTGGGTAAAACAAAAAACCTAAAAGATGCCGAAACGTCGTCGCTGGGG‑GAAATTATCTACCATACGAAGtttgc                                                                  >  2:257950/1‑248 (MQ=255)                                                                                                                                                                                         aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGAG‑AATTTTTTCCA‑ACAAATAAAAAAAATGGAG‑GTG‑TCCTGATGAGCGAAAGGAATAA‑ACAAAAGGCCGGGAA‑TAAGCTG‑GAAGAGATTGTTGCTCAATTTAACGCCCTGATTTTTTCCCGCAAACCTGATGACGGGGGGGATAAACTAAAAAAACTAAAGGATGCCGAAACATCGTCGATGGGGGAAAAtttttaacatccgaagttttaca                                                                 >  1:631108/1‑229 (MQ=255)                                                                                                                                                                                          aaTATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑TCAGACAAATAAAAAAACTGGAG‑GTG‑CCATGATGAGCGAAAGTAATAA‑ACAACAGGCAGTGAA‑AAAGGTG‑ACAGAGATTGTCGCTAAATTTACCCCCATGATTTCTACCCGAATGCCTGATGACGTGGGGGATAAAATAAAAAAGCTCAAGGATGCCGAAACGTCGTCGATGGGG‑AAAAATATCTACCATACGATGTTCGacaac                                                              >  1:453808/1‑251 (MQ=255)                                                                                                                                                                                             ataAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACAt                                                            <  1:609109/250‑1 (MQ=255)                                                                                                                                                                                               aaaaCCACATTTTTAGTGGTAGTTTGTGG‑GGGTG‑AATTTTT‑CCAGAAAAATAAAAAAACTGGAG‑TTG‑CCATGAGGAGCAAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAc                                                                                                          <  2:160694/203‑1 (MQ=255)                                                                                                                                                                                               aaaaCCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑TCCGACAAAAACAAAAACTGGGG‑GTG‑CCATGATGAGCGAAAGTAATAA‑ACAAAAGGCAGTGAA‑AAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCCAATGCCTGATGACGTGGTGGGTAAACTAAAACAGCTAAAGGATGCCGAAAc                                                                                                          >  1:160694/1‑203 (MQ=255)                                                                                                                                                                                               aaaaCCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGAAAAATACAAAAAATTGAG‑TTG‑CCCTGATGAGCGGAAGTAATAA‑GCCACAGGCAGTGGA‑TAAGGTG‑ACAGAGAATGTTGCTAAATTTACCGCCATGATTTCTAACCGAAAGCCTGATGACGGGGGGGGGAAAATAAAAAAGATAAAGGAAGGCGAAAAGGCGGCGATGGGG‑GAAATTATTTACCATaagagggtcgaaaaaaagc                                                          >  1:433539/1‑232 (MQ=255)                                                                                                                                                                                                    cacaTTTTTAGAGGGAGTT‑GTGGGCGGTG‑AATTTTT‑CCAAAAAAAAACAAAAACTGGAG‑TTG‑CCAAGCAGAGAGAAA‑TAAAAACGCACCCGGCAGGGAA‑TACGTTG‑AAAGACATTGTCGCTATGTTTGCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAg                                                    <  2:177317/251‑1 (MQ=255)                                                                                                                                                                                                            ttagggttggtggtggggggTG‑AATTTTT‑CAAAAAAAATACAAAAACGGGTG‑TTG‑ACATGAGGAGCGAAATAAATAA‑GCAGCATGC‑GTGAAATAAGTTG‑ACAGAGAGTGTCGCTAACTTAGCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTg                                              <  2:648629/232‑1 (MQ=255)                                                                                                                                                                                                                ggTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAAATGGAG‑GTG‑CCATGATGAGCGCAAGGAATAA‑ACAAAAAGCAGTGAA‑TAAATTT‑TCAGAGATTGTCGCTAACTTTAACCCCCTGATTTCTACCCGAATGCCTGATGACGTGGTGGGTAAACTAAAAAAGCTAAAAGATGCCGAAACCTCGTCGGTGGGG‑GAAATTATCTACCATACGATGTTCGACAAAATGCAAAAAACGATTGACCTg                                         >  1:532099/1‑250 (MQ=255)                                                                                                                                                                                                                           g‑CGGTA‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcc                             <  1:463391/251‑1 (MQ=255)                                                                                                                                                                                                                                           ccAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                >  1:435182/1‑251 (MQ=255)                                                                                                                                                                                                                                            cAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATg                                                           >  2:270750/1‑207 (MQ=255)                                                                                                                                                                                                                                            cAGACAAATACAAAAACTGGAG‑TGG‑CCATGATGGGCGAAAGTAATAA‑GCAACAGGCAGTAAA‑TAATTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATg                                                           <  1:270750/207‑1 (MQ=255)                                                                                                                                                                                                                                              gACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGAGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg             >  2:5358/1‑251 (MQ=255)                                                                                                                                                                                                                                                  aaTACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg             >  2:295328/1‑247 (MQ=255)                                                                                                                                                                                                                                                      cAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                   >  2:39505/1‑237 (MQ=255)                                                                                                                                                                                                                                                      cAAAAACTGGAG‑TGG‑CAAGGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                   <  1:39505/237‑1 (MQ=255)                                                                                                                                                                                                                                                        naaaCTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGCGATTATGtt   >  2:310576/2‑251 (MQ=255)                                                                                                                                                                                                                                                         aaccTGGAG‑TTC‑CCAGAATGAGCAAAATAAATAA‑GAAACAGGAAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc  <  2:210955/248‑1 (MQ=255)                                                                                                                                                                                                                                                         aaaCTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc  >  1:162548/1‑251 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                              ACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AATTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTC  >  NC_000913/3206204‑3206699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑