Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	33.3%	54.6 / 25.6	49	intergenic (‑179/‑28)	ttdR/ttdA	DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (6/26);  new base T (16/0);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 3.31e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCG  >  NC_000913/3206197‑3206685                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                 gCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCAAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAAATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGCG‑AA‑ATTTT‑CCAgtaaaaaac                                                                                                                                                                                                                                                       >  1:655260/1‑242 (MQ=255)    gtgAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTGTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACaaaa                                                                                                                                                                                                                                                   <  2:275781/251‑1 (MQ=255)        aCGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTg                                                                                                                                                                                                                                               <  1:433087/251‑1 (MQ=255)         cGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCGTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCGCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CTGTG‑AA‑TTTTT‑CCAGGCAAATACAAAAACTgg                                                                                                                                                                                                                                              <  2:655260/251‑1 (MQ=255)              tCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTACTTATCCGCAGTTGGGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGGG‑AAATTTTT‑cgaaaaaaaaaaaaaaactgaggtg                                                                                                                                                                                                                                          >  2:441444/1‑227 (MQ=255)                 aCCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTAATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑cc                                                                                                                                                                                                                                     <  2:197805/250‑1 (MQ=255)                  ccAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGGGTAA‑TTTTT‑CC‑GACAAAAAAAAAAAATGGAGGTTG‑cc                                                                                                                                                                                                                                     >  2:87975/1‑250 (MQ=255)                       aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑TCACACAAATACAAAAACTTGAG‑TTG‑CCatgatg                                                                                                                                                                                                                               >  1:641957/1‑250 (MQ=255)                              aGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAg                                                                                                                                                                                                                       <  1:419410/251‑1 (MQ=255)                               gTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGt                                                                                                                                                                                                                      <  1:670839/251‑1 (MQ=255)                                   ttGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑GA‑TATTT‑TCAGAAAAAAAAAAAAAATGGGG‑GTG‑CCatgggagggggaagaaat                                                                                                                                                                                                                   >  2:458099/1‑235 (MQ=255)                                          aaGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑cc                                                                                                                                                                                                                                     <  2:17711/225‑1 (MQ=255)                                                                       ccGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCg                                                                                                                                                                           <  1:127530/251‑1 (MQ=255)                                                                        cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑GA‑TTTTT‑TCAGACAAATAAAAAAAATGGAG‑GTG‑GCATTATGAGCGAAAGGAAAAA‑GCAAAAAGGAGGGAA‑TAAGGTG‑Accaagaatgtcgg                                                                                                                                                                          >  1:666975/1‑239 (MQ=255)                                                                                 gATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTa                                                                                                                                                                  <  1:207806/250‑1 (MQ=255)                                                                                  aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑TCAGGCAAAAAAAAAAAATGGAG‑TTG‑CCATGATGAGCGGAAGAAAAAA‑ACAAAAAGCAGTGAA‑TAAGTTG‑AAAGAGATTGTCGCTAACTTTaac                                                                                                                                                                >  1:191587/1‑249 (MQ=255)                                                                                               aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCCGACAAATACAAAAAATGGAG‑TTG‑GCATGATGAGGGGAAGGAAAAA‑GCAACAGGCAGGGAA‑TAAGTTG‑AAAGAGATTTTCGCTAACTTTTCCCCCATGATTTCtt                                                                                                                                                   >  1:468477/1‑250 (MQ=255)                                                                                                            ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑TCCGACAAAAAAAAAAAATGGGG‑GTG‑CCATGATGGGGGAAAGTAATAA‑GCAACAGGGAGTGAA‑AAAGTTG‑ACAGAGAGTGTCGCTAACTTTTCCCCCCTGATTTTTAccccgaagcctgg                                                                                                                                      >  1:210955/1‑241 (MQ=255)                                                                                                             tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑GA‑TTTTT‑TCCGACAAAAACAAAAAATGGAG‑TTG‑GCCTGATGAGCGGAAGAAAAAA‑ACAACAGGCAGTGAA‑TAAATTGGACA‑AGATTTGCGGCAAATTTACCGCCCTGATTTTTAccccaaagcccggt                                                                                                                                     >  1:634344/1‑240 (MQ=255)                                                                                                                        gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑TCAGACAAATACAAAAAATGGAG‑GTG‑CCATGATGAGCGAAAGGAAAAA‑AAAACAAGCCGTGAA‑TAAAATG‑ACAGAGATTGTCGCCAAATTTACCCCCCTGATTTTTACCCCCAAGCCTGATGACgcgggggg                                                                                                                          >  1:404075/1‑244 (MQ=255)                                                                                                                           ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑ATTTT‑TCAAAAAAAAACAAAAACCTGAG‑TTG‑CCCTGGTGAGAGAAAAGAATAA‑ACAACAAGCAGAGAA‑AAAATTG‑ACAAAGATTTTCGCTAACGTTACAGCCACCTATTCTACCCGAATGCCCGATGAccggggggaga                                                                                                                        >  2:223879/1‑240 (MQ=255)                                                                                                                           cgggCGAAATATTCATTCTTATTTCCGCGTTGATTCACGAATTGTTAATTACATTTTCAGGGTGGAAAAAAATAAAAAACCAAATTTTGAGTGGTAGTTTGGGG‑GGGTG‑AA‑TTTTT‑CAAGAAAAATAAAAAAACTGGAG‑TTG‑CCAGGATGAGCAAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                                                                                                                         <  2:641957/247‑1 (MQ=255)                                                                                                                              gcgcAATATTCATTCTTATTTTCGGGTTGATCCACAAACTGTTAATTAAATATTCAGGGTGGAAAAAAATAAAAAACCACATTTTAAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATAAAAAAACGGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAGGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGATGTGGTGGATAAACt                                                                                                                    <  1:62517/251‑1 (MQ=255)                                                                                                                                gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATACCTGATGACGTGGTGGATAAACTaa                                                                                                                  <  1:12517/251‑1 (MQ=255)                                                                                                                                                   tCGCGTTAATTCACGAACTTTTAATTAAATTTTCAGGGTGGAAAAAAATAAAAAACCACATTTTTAGGGGTAGTTTGGGG‑CGGTG‑AA‑TTTTT‑CAAAACAAAAAAAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAAAAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCg                                                                                                <  2:404075/250‑1 (MQ=255)                                                                                                                                                       gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑ATTTT‑TTAAACAAAAAAAAAAAAAGGAG‑TTGGCCA‑GATGAGCGAAAATAATAA‑ACAAAAAGCCGTGAA‑AAAGTTG‑ACAGAGAATGGTGCTCACTTTATCGCCACGCTTTTTACCCGAATGCCTGATGACGGGGTGGATAAAATAAAAAAGATAAAGGATGCCGaaaa                                                                                            >  2:209263/1‑249 (MQ=255)                                                                                                                                                            ttCACGAACGTTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAACCTGGAG‑TTG‑CCATGATGAGCGAAATTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGGCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcgt                                                                                       <  1:663496/250‑1 (MQ=255)                                                                                                                                                             tCACGAACTGTTAATTACATATCCACGGGGAAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTA‑AA‑TTTTT‑CCAGACAAATACAAAAACGGGAT‑TTC‑CCATGAGGATCAAAAGTAATAG‑GCAACAGGCAGTGAA‑TATGTTG‑ACATAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACgtcgtc                                                                                      <  1:266322/250‑1 (MQ=255)                                                                                                                                                              cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑TCCGACAAATACAAAAAATGGAG‑GTG‑CCATGATGAGCGGAAATAAAAA‑ACAACAAGCAGGGAA‑AAAGTTG‑ACAGAGAATGTCGCTAAATTTACCGCCATGATTTTTACCCGAAAGCCTGATGACGGGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGAAACGtcgtcgg                                                                                    >  1:56069/1‑250 (MQ=255)                                                                                                                                                               ccGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGG‑CGGTG‑AA‑TTTTT‑CCAGAAAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCAAAAGAAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAt                                                                                   <  1:515193/250‑1 (MQ=255)                                                                                                                                                               aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑TCCGACAAATACAAAAAATGGAG‑GTG‑CCATGATGAGCGGAAATAATAA‑GCAAAAGGCAGTGAA‑TAAATTG‑TCAGAGATTTTCGCTAACTTTACCGCCCTGATTTCTTCCCCAATTCCTGGTGACGTGGTGGGTAAAATAAAAAAACTAAAAGATGCCGCAAAGTCGTCGa                                                                                    >  1:286117/1‑250 (MQ=255)                                                                                                                                                                  aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATggg                                                                                <  1:87975/251‑1 (MQ=255)                                                                                                                                                                       taaaTTACAATTTCACGGTGGAAAAAAATATAAAACCCCATTTTTAGGGGTAGTTTGTGG‑GGGTA‑AA‑TTTTT‑CCAGAAAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑AAAGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑aaa                                                                           <  1:380846/248‑1 (MQ=255)                                                                                                                                                                            tataggttCACGGGGGCAAAAAATAAAACACCTGATTTTTAGTGGTAGTTTGTGG‑GGGTG‑AA‑TTTTT‑CCAAACAAATACAAAATCGTGAG‑TTG‑CCTTTTTGTGCGAAGGTAATAA‑GCAACAGGAAGTGAA‑TAGGTTG‑ACAGTGATGGTCGCAATCTGGACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTAt                                                                      <  2:224165/244‑1 (MQ=255)                                                                                                                                                                               atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑TCAAACAAATAAAAAAAATGGAG‑TTG‑GCATGATGAGCGAAAGTAATAA‑ACAAAAAGCAGTGAA‑TAAGTTG‑ACAGAGAATGTCGCTAAATTTAACGCCATGATTTTTATCCCAATGCCTGATGACGCGGGGGGGAAACTAAAACAGCTAAAAGAAGCCGAAACAGCGGCGATGGGG‑AAAAATATTTAc                                                                  >  1:450752/1‑251 (MQ=255)                                                                                                                                                                                    cACGGTGGAAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGGGG‑CGGTG‑AA‑TTTTC‑CAAAAAAAATAAAAAAACTGGAG‑TTG‑CCATGAGGAGCGAAAGTAATAA‑GCAACAGGCAGTAAA‑TAAGTTG‑ACAGAGATTTTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATAc                                                             <  1:377765/251‑1 (MQ=255)                                                                                                                                                                                       gggggaaaaaaaaaaaaaacaccaTTTTAAGTGGTATTTTGGGG‑GGGTG‑AA‑TTTTT‑ACAAAAAAATAAAAAAATGGGAT‑GGG‑CAATGATGAGAGAAAGAAAAAA‑GAAAAAGGCAGTAAT‑TAAGTTG‑AAAGAGATTGGCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGAt                                                          <  2:274668/229‑1 (MQ=255)                                                                                                                                                                                       ggTGGCAAAAAATAGAAACCCACATTTTTAGTGGTAGTTTGTGG‑CGGTA‑AG‑TTTTT‑CAAGACAAATAAAAAACCTGGAG‑TTG‑CAATGAGGGGCAAAAGTAATAA‑GCAAGAGGCAGTGAA‑‑AAATTGCACAGAGGTTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGAt                                                          <  2:332429/251‑1 (MQ=255)                                                                                                                                                                                             aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑TCAGACAAAAACAAAAAATGGAG‑GTG‑CCATGATGAGCGGAAGAAATAA‑GCAACAGGCAGTGAA‑TAAATTG‑ACAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTACCCCAAAGCCTGATGACGGGGGGGATAAAATAAAAAAGCTAAAGGATGCCGAAACGGCGTCGGTGGGG‑GAAATTATCTAACATACGATGtttgg                                                    >  1:120690/1‑248 (MQ=255)                                                                                                                                                                                             aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGGG‑AA‑ATTTT‑TCAAACAAAAAAAAAAAATGGAG‑TTT‑GCATGATGGGCGAAAGGAATAA‑ACAAAAAGCCGTGAA‑AAAAATG‑ACAGAGAATGTCGCTAACTTTACCCCCCCGATTTCTACCCCAATGCCCGATGAAGGGGTGGGTAAAACAAAAAACCTAAAAGATGCCGAAACGTCGTCGCTGGGG‑GAAATTATCTACCATACGAAGtttgc                                                    >  2:257950/1‑248 (MQ=255)                                                                                                                                                                                                aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGAG‑AA‑TTTTTTCCA‑ACAAATAAAAAAAATGGAG‑GTG‑TCCTGATGAGCGAAAGGAATAA‑ACAAAAGGCCGGGAA‑TAAGCTG‑GAAGAGATTGTTGCTCAATTTAACGCCCTGATTTTTTCCCGCAAACCTGATGACGGGGGGGATAAACTAAAAAAACTAAAGGATGCCGAAACATCGTCGATGGGGGAAAAtttttaacatccgaagttttaca                                                   >  1:631108/1‑229 (MQ=255)                                                                                                                                                                                                 aaTATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑TCAGACAAATAAAAAAACTGGAG‑GTG‑CCATGATGAGCGAAAGTAATAA‑ACAACAGGCAGTGAA‑AAAGGTG‑ACAGAGATTGTCGCTAAATTTACCCCCATGATTTCTACCCGAATGCCTGATGACGTGGGGGATAAAATAAAAAAGCTCAAGGATGCCGAAACGTCGTCGATGGGG‑AAAAATATCTACCATACGATGTTCGacaac                                                >  1:453808/1‑251 (MQ=255)                                                                                                                                                                                                    ataAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACAt                                              <  1:609109/250‑1 (MQ=255)                                                                                                                                                                                                      aaaaCCACATTTTTAGTGGTAGTTTGTGG‑GGGTG‑AA‑TTTTT‑CCAGAAAAATAAAAAAACTGGAG‑TTG‑CCATGAGGAGCAAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAc                                                                                            <  2:160694/203‑1 (MQ=255)                                                                                                                                                                                                      aaaaCCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑TCCGACAAAAACAAAAACTGGGG‑GTG‑CCATGATGAGCGAAAGTAATAA‑ACAAAAGGCAGTGAA‑AAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCCAATGCCTGATGACGTGGTGGGTAAACTAAAACAGCTAAAGGATGCCGAAAc                                                                                            >  1:160694/1‑203 (MQ=255)                                                                                                                                                                                                      aaaaCCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGAAAAATACAAAAAATTGAG‑TTG‑CCCTGATGAGCGGAAGTAATAA‑GCCACAGGCAGTGGA‑TAAGGTG‑ACAGAGAATGTTGCTAAATTTACCGCCATGATTTCTAACCGAAAGCCTGATGACGGGGGGGGGAAAATAAAAAAGATAAAGGAAGGCGAAAAGGCGGCGATGGGG‑GAAATTATTTACCATaagagggtcgaaaaaaagc                                            >  1:433539/1‑232 (MQ=255)                                                                                                                                                                                                           cacaTTTTTAGAGGGAGTT‑GTGGGCGGTG‑AA‑TTTTT‑CCAAAAAAAAACAAAAACTGGAG‑TTG‑CCAAGCAGAGAGAAA‑TAAAAACGCACCCGGCAGGGAA‑TACGTTG‑AAAGACATTGTCGCTATGTTTGCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAg                                      <  2:177317/251‑1 (MQ=255)                                                                                                                                                                                                                   ttagggttggtggtggggggTG‑AA‑TTTTT‑CAAAAAAAATACAAAAACGGGTG‑TTG‑ACATGAGGAGCGAAATAAATAA‑GCAGCATGC‑GTGAAATAAGTTG‑ACAGAGAGTGTCGCTAACTTAGCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTg                                <  2:648629/232‑1 (MQ=255)                                                                                                                                                                                                                       ggTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAAATGGAG‑GTG‑CCATGATGAGCGCAAGGAATAA‑ACAAAAAGCAGTGAA‑TAAATTT‑TCAGAGATTGTCGCTAACTTTAACCCCCTGATTTCTACCCGAATGCCTGATGACGTGGTGGGTAAACTAAAAAAGCTAAAAGATGCCGAAACCTCGTCGGTGGGG‑GAAATTATCTACCATACGATGTTCGACAAAATGCAAAAAACGATTGACCTg                           >  1:532099/1‑250 (MQ=255)                                                                                                                                                                                                                                  g‑CGGTA‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcc               <  1:463391/251‑1 (MQ=255)                                                                                                                                                                                                                                                   ccAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg  >  1:435182/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                 GCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGG‑CGGTG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGAG‑TTG‑CCATGATGAGCGAAAGTAATAA‑GCAACAGGCAGTGAA‑TAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGG‑AAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCG  >  NC_000913/3206197‑3206685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑