Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,362,045	T→C	intergenic (‑111/+506)	cadC ← / ← pheU	DNA‑binding transcriptional activator CadC/tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,362,045	0	T	C	100.0%	87.2 / NA	27	intergenic (‑111/+506)	cadC/pheU	DNA‑binding transcriptional activator CadC/tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/17);  total (10/17)

ACTGTGTTGAGCAAAGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTTTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACC  >  NC_000913/4361803‑4362289                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     aCTGTGTTGAGCAAAGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGc                                                                                                                                                                                                                                              <  1:64044/251‑1 (MQ=255)    gtgtagatcAAAGAAAACCAGAAGATCGATTAATCTCGGCTAAAGGGTAAGGTGACGCCCATTACGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTTCAGGTTTTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATTGGTTTATTTACATATCATTGGTGAATGCAAGACGTTAGTCTTACCAGCCag                                                                                                                                                                                                                                           <  2:71302/243‑2 (MQ=255)    gtgtTGAGCAAAGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGcc                                                                                                                                                                                                                                             <  1:28751/249‑1 (MQ=255)            cAAAGCAAACCAGACGATCGCTTAATATCGGGTCAAGGGTCAGTTGACGCACATTGCGGCTAATTTGGCTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTAAAGGTTGTTGCATAATAGAAAATCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGACTGCAAGAAGTTATTCTTACCAGCCATAAActtct                                                                                                                                                                                                                                   >  2:400127/1‑251 (MQ=255)                 aaaaCCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAAGTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGAtt                                                                                                                                                                                                                              <  1:7507/251‑1 (MQ=255)                                aTTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGaa                                                                                                                                                                                                               <  1:292012/251‑1 (MQ=255)                                                  gTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAAcc                                                                                                                                                                                             <  1:98887/251‑1 (MQ=255)                                                  gTAAGTTGACGCACATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCTTTCGCCCACGCGACCTACAGGTTGTTGCATAATAGAAACTCATTCGTAAAGGGGATGATGCAATGTTAATTGCCACAACCTATTTTTACAATCTATAGCTGGGTTTATTTACATATTATTGGTGAATTCAAGACGTTATTCTTACAAGCCATAAATTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAAcc                                                                                                                                                                                             >  2:363670/1‑251 (MQ=255)                                                         gACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTa                                                                                                                                                                                      <  2:121224/251‑1 (MQ=255)                                                                                   aTGGACGGAGTCACAAGCCATTCGCCAACGCGCACTACAGGTAGTTGCATAATAGAAACTCATTCGAAAAGGGACTGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGCTGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATtcatcc                                                                                                                                                            >  2:58455/1‑250 (MQ=255)                                                                                        cGGAGTAAAAAGCCATTCGCCAACGCGAACGACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCttt                                                                                                                                                       <  2:321723/251‑1 (MQ=255)                                                                                         ggAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTg                                                                                                                                                      <  1:311777/251‑1 (MQ=255)                                                                                                            cAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCGATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATGCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATAGTCCGTACCATCAAAATTCATCACCTTTGCCAAAGAAAAATGTTCAGa                                                                                                                                   <  1:363670/251‑1 (MQ=255)                                                                                                                gcgAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGACACAACCTATTTTGACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAaat                                                                                                                               <  2:305095/251‑1 (MQ=255)                                                                                                                gcgAACGACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTAAAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAaat                                                                                                                               <  2:206842/251‑1 (MQ=255)                                                                                                                                      ataGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCgg                                                                                                          >  1:144928/1‑250 (MQ=255)                                                                                                                                          aaaCTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCAc                                                                                                     >  1:240232/1‑251 (MQ=255)                                                                                                                                               cATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCAGCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACt                                                                                                <  1:55737/251‑1 (MQ=255)                                                                                                                                                           gAATGATGCAATGATAATTGCCCCAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTGCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGAt                                                                                    <  1:20503/251‑1 (MQ=255)                                                                                                                                                                    aaTGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATCCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCAGGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCt                                                                           <  1:216319/251‑1 (MQ=255)                                                                                                                                                                        aTAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCACGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGACAAATGTTCAGAAACTAATCCATGGAAAATTGTCCGGCGCACTTACTATTTTAATGGATTGTTAGTCTTTGc                                                                       >  2:21728/1‑251 (MQ=255)                                                                                                                                                                                     ccTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACTTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTTCCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGGCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGaa                                                          <  1:407748/251‑1 (MQ=255)                                                                                                                                                                                       tATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACt                                                        >  1:198661/1‑251 (MQ=255)                                                                                                                                                                                                                    taCATATTATTGGTGAATGAAAGACGTTCTTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGa                            >  2:50551/1‑250 (MQ=255)                                                                                                                                                                                                                               ggTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtcatc                  >  2:184485/1‑249 (MQ=255)                                                                                                                                                                                                                                   aaTGCAAGACGTTATTCTTACCAGCCATAACCTTCTTGATTACATAGGATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGc            >  2:172899/1‑251 (MQ=255)                                                                                                                                                                                                                                             gTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGAcc  <  2:198661/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     ACTGTGTTGAGCAAAGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTTTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACC  >  NC_000913/4361803‑4362289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑