Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	498,116	C→T	P21L (CCT→CTT)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	498,116	0	C	T	100.0%	189.6 / NA	55	P21L (CCT→CTT)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/21);  total (34/21)

GGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCT  >  NC_000913/497876‑498358                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   gggAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATGCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATTCCCCCTCACTTGAAGCGGTa                                                                                                                                                                                                                                          <  1:137097/251‑1 (MQ=255)     aCAGGCCCGCACACGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAAc                                                                                                                                                                                                                                      >  1:338452/1‑251 (MQ=255)     aCAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAAc                                                                                                                                                                                                                                      <  1:139478/251‑1 (MQ=255)                 aaGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTATCACAGCAATTAGGTCTTCTTCCTCATTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGTTAATGCGACCGACTAAAACCGGTATCCTGCTTGCCAACCTGGGTACGCCCTATGCCCCCACACTTGACGCGGTAACACGcattcttaaac                                                                                                                                                                                                                          >  2:112771/1‑241 (MQ=255)                   gCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACa                                                                                                                                                                                                                         >  1:109354/1‑250 (MQ=255)                     aaaCTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTATTGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACCCTCTCTGTAACAAtt                                                                                                                                                                                                                      >  2:21827/1‑251 (MQ=255)                                 aaTCTCAGGCCGGATCTTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTTGTTCTTCTTCCTCACTTTTCAGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTCATGCGTCAGACTTAAACCGGTATCCTGCTGGCCAACCTGGGTACGCCCGATGCACCCACACTTGAAGCGGTCACACGCTATCTGAAACCATTTTTaaacgcc                                                                                                                                                                                                            >  2:368142/1‑244 (MQ=255)                                      cAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAAGTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGa                                                                                                                                                                                                         <  1:1576/247‑1 (MQ=255)                                        ggCCGGATCTTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCCCACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGCCAGACGCGTg                                                                                                                                                                                                   >  1:354913/1‑251 (MQ=255)                                                                 cAAAAACATCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGAATAAAACAGGTATCCTGCTGGCAAACCTGGGTACGCCAGATGCCCCCACACTTGAAGAGGGAAAACGCTATCTGAAACCATTTTTAAGCGACAGACGCGTGGtagctatctaacggcagatattgg                                                                                                                                                                          >  2:245918/1‑228 (MQ=255)                                                                   aaaaCCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTGTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTAtggtgg                                                                                                                                                                        <  1:219774/251‑1 (MQ=255)                                                                     aaCCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGcc                                                                                                                                                                      >  1:48924/1‑251 (MQ=255)                                                                               tGCGCTATTCCTATTCCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGCGGCGGTAATGCGTCAGACTAAAACCGTTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGCAACAATTTTTAAGCGACCGACGCGTGGTTGATACCCCACGGGTGTTATGGTGGCCATTGGTgcgg                                                                                                                                                            >  1:324250/1‑250 (MQ=255)                                                                                        ccTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGCGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGAtt                                                                                                                                                   >  1:38731/1‑251 (MQ=255)                                                                                             ttCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGTCCAGTGCTGCGCGGCGTGATTTTGc                                                                                                                                               <  1:339724/250‑1 (MQ=255)                                                                                              tCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGcc                                                                                                                                              <  1:124799/250‑1 (MQ=255)                                                                                                      gACCTCTCACAGCAATTAGTTCTTATTCCGCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGGAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGTTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTTATACCTCACGTTTGTTATGGTGGCCATTGCTGCGCTGCGTTATTTTGCCGCTGCGCTc                                                                                                                                     <  2:377988/251‑1 (MQ=255)                                                                                                         ctctCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTc                                                                                                                                     <  2:89641/248‑1 (MQ=255)                                                                                                         ctctCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTc                                                                                                                                     >  1:89641/1‑248 (MQ=255)                                                                                                                     ttAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGa                                                                                                                       >  1:96014/1‑250 (MQ=255)                                                                                                                        gTTCTTCTTCCTCACTTTTCCGCTACACTTATCAACAAGTTGAATCGATA‑AGCGGCGGTAATGCGTCAGACTAAAACCGGGATCCTGCTGGCAAACCTGGGTACGCCAGATGCACCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGACCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTAGCCGCGTGTGGCGGAGCt                                                                                                                   >  1:361527/1‑251 (MQ=255)                                                                                                                          tcttcttcCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATCCCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTTCCGCTGAGCTCGCCGCGTGCGGCGAAggcgt                                                                                                                 >  2:305059/1‑247 (MQ=255)                                                                                                                            ttcttcCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTTGCGGAGCTggat                                                                                                               >  2:339009/1‑248 (MQ=255)                                                                                                                                 ccTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCTAAGCTGTATGCctc                                                                                                          <  1:107441/251‑1 (MQ=255)                                                                                                                                    catttttCCGTTCCAATTATCAACCAGTTGAATCG‑TACAGAGGCGGTAATGCGTCAGAACAAAACCGGTATCCTGCGTGCAAACCTGGGGACGCCCGATGCCCTCACACTTTAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCTACAGCCGCGTGGTTGATACCCCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGTCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCtctgt                                                                                                       <  2:228236/248‑1 (MQ=255)                                                                                                                                         ttCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATGTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCtgg                                                                                                   <  2:104034/250‑1 (MQ=255)                                                                                                                                            cGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCtggatgg                                                                                               <  1:94246/251‑1 (MQ=255)                                                                                                                                            cGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCtggatgg                                                                                               >  1:243077/1‑251 (MQ=255)                                                                                                                                                  aaTTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAAACGGTATACTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTCAGCGACCGACGCGTGGTTGATACCTCCCGGTTGTTATGGTGGACATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGACGCTGTTTGCCtctgtattgctggacggtg                                                                                         >  2:253311/1‑237 (MQ=255)                                                                                                                                                         aacaaGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCCCACTTGAAGCGGTAACACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGCCTGGATGGAAGGTGGCTcgcc                                                                                  >  1:309459/1‑251 (MQ=255)                                                                                                                                                         aacaaGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTcgcc                                                                                  <  2:380463/251‑1 (MQ=255)                                                                                                                                                           caaGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTcgccgc                                                                                >  1:213416/1‑251 (MQ=255)                                                                                                                                                             aggttaaTCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTg                                                                              <  2:354913/246‑1 (MQ=255)                                                                                                                                                             aGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGGGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCTGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGTTTAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTg                                                                              <  1:71575/251‑1 (MQ=255)                                                                                                                                                                   aTCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACCCTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTGTTCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGTTGGtt                                                                        >  2:201658/1‑251 (MQ=255)                                                                                                                                                                             gggCGGAAATGCGTCAGACTAAAACCGGTATCCTGCGGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGTTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGGCTGTATGGAAGGTGGCTCGCCGCTGATGGTTTACAgccgc                                                               <  2:146468/250‑1 (MQ=255)                                                                                                                                                                              ggCGGTAATGCGTAAGACTACAACTGGTATCCTGCTGGCAACCCCGGGTCGGCCAGATGCACCCACACTTGAAGCGGAAAGACGCTATCTGACACAATTTTTAAGAGACAGACGCGTGGTCGATACCTCTCGGTTGCTATGCTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGAGTGGCGACACTGTATGCCTCTGACTGGTTTGAAGGTGGCTCGCCGCTGGTGGTTTTCAtccgcc                                                              >  2:396720/1‑251 (MQ=255)                                                                                                                                                                                      tGCGTCAGACTCACAACGGCATCCTGCTGGCAAACCTGGGCACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAACCACTTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGACTCTGTCTGGATGGAAGGTGGCTCGACGCTGATGGTTTACAGCCGCCAGCAAccg                                                      >  2:213710/1‑249 (MQ=255)                                                                                                                                                                                        cGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAAcagc                                                     >  1:119499/1‑250 (MQ=255)                                                                                                                                                                                         gtcagactaccaaaggTATCCTGCTGGCCAACATCGGAACAACCGATGCACCCACACTTGACGCGGTAAAACGCTATCTGACACCATTTTTAAGCGCCAGACGCGCGGTTGATAACTAACAGTTGCTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGATCTCGCCGCGTGTGGCGAAGATGTATGCCTCTGTCTGGATGGAAGGTGGCTCGTCGCTGATGGTTTACAGCCGCCCGAAAcagc                                                     >  2:192623/15‑249 (MQ=255)                                                                                                                                                                                         gTCAGACTAAACCCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTGTCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGACGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAAcagc                                                     >  2:283498/1‑249 (MQ=255)                                                                                                                                                                                         gTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCCCACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAAcagc                                                     >  2:174418/1‑249 (MQ=255)                                                                                                                                                                                                 aaaaCCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTg                                             <  1:253311/249‑1 (MQ=255)                                                                                                                                                                                                     ccGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGcaca                                        >  1:143591/1‑250 (MQ=255)                                                                                                                                                                                                      cGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAAc                                      <  2:109354/251‑1 (MQ=255)                                                                                                                                                                                                          aTCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGttt                                  >  1:117383/1‑251 (MQ=255)                                                                                                                                                                                                                ctgGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACCGCAGGCGCTGGCACAACGTTTACCggg                            >  2:302878/1‑250 (MQ=255)                                                                                                                                                                                                                   gCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACCGCAGGCGCTGGCACAACGTTTACCgg                             >  2:44498/1‑247 (MQ=255)                                                                                                                                                                                                                     aaaCCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGc                       >  1:383893/1‑251 (MQ=255)                                                                                                                                                                                                                       aCCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGTTTGATACCTCACGGTTTTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTTCGCTCGCCGCGTGTGGCGAAGCTGTATGCCGCTGTCTGGATGGAAGGTTGCGCGCCGCTGATGGTTTACAGCCGCCAGCATCAGCAGGCGCTGGCACAACGTTTACCGGAGATGccc                     <  1:204633/251‑1 (MQ=255)                                                                                                                                                                                                                         cTGGGTACGCCCGAAGCCCCCACACTTGAAGCGGTAAAACGCTCTCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGATATGGTGGCCATTGATGCGCGGCGAGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAACTGTATGCCACTGTCTGGATGGAAGGGGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCCGAACGTTTACCGGAGATGGCCgg                   >  2:78276/1‑250 (MQ=255)                                                                                                                                                                                                                           gggTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGACGCCCGt                   >  2:7280/1‑249 (MQ=255)                                                                                                                                                                                                                             gTACGCCCGATGCCCCCACCCTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCGGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAgcg               <  2:48924/251‑1 (MQ=255)                                                                                                                                                                                                                                     gATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAAt       <  1:55898/251‑1 (MQ=255)                                                                                                                                                                                                                                          ccccACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGCAGCGCTGGGAATGAGcg  >  2:31017/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   GGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATA‑AGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCT  >  NC_000913/497876‑498358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑