Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,123,930	A→T	V176E (GTA→GAA)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,930	0	A	T	100.0%	260.2 / NA	69	V176E (GTA→GAA)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/44);  total (25/44)

GCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTG‑ATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTG  >  NC_000913/4123687‑4124176                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                           gcCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGGGACACAGCGGCATCTCTTCGGGCCCGGCTATACAGCCAATCCGTTTATTCCCTTGCTCATATAAATAATTTTCTGcctc                                                                                                                                                                                                                                                     >  1:2918/1‑248 (MQ=255)      cacaTCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATg                                                                                                                                                                                                                                                <  1:93347/251‑1 (MQ=255)       ccaTCGCTATGGCAGAAGACAGCATTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTTCTCATATAAATAATTTTCTGCATCAAATgc                                                                                                                                                                                                                                               <  2:57434/250‑1 (MQ=255)       acaTCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATgc                                                                                                                                                                                                                                               <  2:325420/251‑1 (MQ=255)       acaTCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATgc                                                                                                                                                                                                                                               <  1:385302/251‑1 (MQ=255)        caTCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATgcg                                                                                                                                                                                                                                              <  1:87993/251‑1 (MQ=255)               aTGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑Tc                                                                                                                                                                                                                                       <  1:191241/250‑1 (MQ=255)               aTGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑Tc                                                                                                                                                                                                                                       <  2:348180/250‑1 (MQ=255)                 ggCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑a                                                                                                                                                                                                                                   <  1:304212/251‑1 (MQ=255)                    agaagaCAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCCTCAAATGCGGCGG‑GCAggatt                                                                                                                                                                                                                                 >  1:312450/1‑247 (MQ=255)                    agaagaCAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCCGCCTCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAAATTTCTGCATCAAATGCGGCCG‑TCAG‑Attt                                                                                                                                                                                                                                >  2:101846/1‑250 (MQ=255)                         aCAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGat                                                                                                                                                                                                                           <  1:138619/251‑1 (MQ=255)                           agcagTAGGCGTTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAAGAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGatat                                                                                                                                                                                                                         <  1:280005/251‑1 (MQ=255)                             cagTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTGGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCAGAGAAATAATGTTCTGCATCa                                                                                                                                                                                                                                                    <  2:406136/224‑1 (MQ=255)                             cagTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGCGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACCGCGGCCTCTCTTCGGGACCGGCTATACAGCCCATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCCTCa                                                                                                                                                                                                                                                    >  1:406136/1‑224 (MQ=255)                                       ttGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCCTCAAATGCGGCGG‑GCAG‑ATTTTCGatattaaatgttggcc                                                                                                                                                                                                             >  1:369462/1‑239 (MQ=255)                                           ggAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGcagctc                                                                                                                                                                                                         <  1:130078/251‑1 (MQ=255)                                             aaGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGcagctcca                                                                                                                                                                                                       <  2:315799/251‑1 (MQ=255)                                             aaGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATAAAGCCCAACCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑GCAG‑AGTGTCGATATGaaaagtaggggcgtccc                                                                                                                                                                                                       >  2:305858/1‑236 (MQ=255)                                                       agcTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGc                                                                                                                                                                                             <  1:353332/251‑1 (MQ=255)                                                           gctgcATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGGAATGAAATTGAGGCAGGTCCAGCCCCGGGGCAAAt                                                                                                                                                                                         >  1:34503/1‑251 (MQ=255)                                                                   gCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑AATTTTGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTTGTTCGc                                                                                                                                                                                 >  1:292701/1‑251 (MQ=255)                                                                   gCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCGGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCGCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATGTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGc                                                                                                                                                                                 <  1:169247/251‑1 (MQ=255)                                                                    cTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATACGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTTTTGATATGAACAGTTGGGAGCTCCCACTCCGGTGCAAAttcggtagcc                                                                                                                                                                                >  1:37810/1‑245 (MQ=255)                                                                           tCCGGTTTCGAAGGTGAAGTCGCCAAGGGCGAGGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCAcc                                                                                                                                                                          <  2:12661/250‑1 (MQ=255)                                                                           tCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCACCCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCAcc                                                                                                                                                                          <  2:75911/250‑1 (MQ=255)                                                                                       ggTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGAt                                                                                                                                                             <  1:227057/251‑1 (MQ=255)                                                                                            aGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATCGCCTTGCAGGCGGTAGTGACACAGCGGCCTCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTTCATCAAATGCGGCGGGTCAA‑TTTGT‑GATTTGCACCGTCGGCCGCTCCAACTCCGGGGCCAATTTGTTCGCCCTCCCCATccggcgccggataagc                                                                                                                                                        >  1:432051/1‑236 (MQ=255)                                                                                                     gggCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCa                                                                                                                                               <  2:369462/251‑1 (MQ=255)                                                                                                         gATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTTCCTCAAATGCGGCGG‑TCCGCATTGTCGAT‑TGAAATATAGGCATCTCCAGCTCCGGGGGAAAATTGTTTGCCCTCACCAACAGGGGCCGATTACGCTGtttcctaatgt                                                                                                                                           >  2:239708/1‑242 (MQ=255)                                                                                                            gTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTg                                                                                                                                         <  1:305858/250‑1 (MQ=255)                                                                                                            gTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTg                                                                                                                                         <  1:410694/250‑1 (MQ=255)                                                                                                              aTTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGACGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCCGCTCCAGCTCCGGTGCAAATTCGTTCTCCATCACCATCGGCGGCAGATTACGCTGTTCCTCCATTCTGGc                                                                                                                                       >  1:170648/1‑250 (MQ=255)                                                                                                                 gCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCa                                                                                                                                   <  1:412781/251‑1 (MQ=255)                                                                                                                   ggATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGGTCGCCATCACCATCGGCGGCAGATTAAGCCGTTCCCCAATGCTGGCATcca                                                                                                                                  >  1:335961/1‑248 (MQ=255)                                                                                                                         aCCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAg                                                                                                                           <  1:183488/251‑1 (MQ=255)                                                                                                                              aaggCCGCAGCGACGCAGCGCCGGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCAGATAAATAATTTTCTGCAGCAAATGCGGCGG‑TCAG‑ATTGTCGGTATGAACGGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCAGCGGTGGCAGAGTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCtt                                                                                                                      <  1:173993/247‑1 (MQ=255)                                                                                                                                   cGCAGCGACGCGGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAAccc                                                                                                                <  2:332070/251‑1 (MQ=255)                                                                                                                                   cGCAGCGACGCAGCGCCTGAACATGGCCGTGCAGGCGGTTGTGGCACCGCGGCATCGCTTCGGGACCGGCTATACAGGCAATCCGGTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCGCCGTTTCAGATTCGTTCGCCATCACCATCTGCTGCAGATTAGGCGGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAAccc                                                                                                                <  1:386614/251‑1 (MQ=255)                                                                                                                                   cGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAAccc                                                                                                                <  2:2918/251‑1 (MQ=255)                                                                                                                                         gACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGcaac                                                                                                          <  1:232661/251‑1 (MQ=255)                                                                                                                                              aGCGCCTGAACATAGCCTTGCAGCCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCAAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCAGCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGACATCACCATCGGCGGCAGATTACGCTGTTCCCCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACAt                                                                                                     <  2:107434/251‑1 (MQ=255)                                                                                                                                                   cTGAACATAGACTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTAGGTATGGACTGGAGGCCGCTCCAACTCCGGGGCAAATTCGGTAGCCATCACCCTCGGCGGCAGATTTCGCTGTTTCTCCATGCTTGCATCCAACGGCCG‑CCTTGAACCCAGCAACAACCTGCCAt                                                                                                >  1:115952/1‑251 (MQ=255)                                                                                                                                                     gAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATc                                                                                               <  1:20537/250‑1 (MQ=255)                                                                                                                                                       aCATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAAt                                                                                            <  1:423765/251‑1 (MQ=255)                                                                                                                                                                tgcaggattatgTGACACAGCGGCGCCTCTGCGGGACCGGCTATACAGCCAATCCGGGTATGCCCTTGCTCATATAAATAATTGTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCATCTCCAGCTCCGGTGTAAATTCTTTCGCCATCACCATCGGCGGCAGAGTACGCTGTGCCTCAATGCTGGCATCAAACGTCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGcgtg                                                                                     <  2:89488/238‑4 (MQ=255)                                                                                                                                                                 gCAGGCGGTAGTGACACAGCGGCATCGCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCGGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGtg                                                                                  <  2:143757/251‑1 (MQ=255)                                                                                                                                                                   aGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCACCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGtgat                                                                                <  1:101846/251‑1 (MQ=255)                                                                                                                                                                       ggTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATc                                                                            <  1:180485/251‑1 (MQ=255)                                                                                                                                                                              acacAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGGGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCCACAACATGCCCTCAATTTTCCTTGTGATGATCCAATCgg                                                                     >  2:110194/1‑250 (MQ=255)                                                                                                                                                                                  aGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAAATGTAGGCCGCTCCAGCTCCGGTGCCAATTCGTTCGCCCTCACCATCGGCGGCAGATTTCGCTGTTTCTCCATGCTGGCACCAAAAGGCCG‑CCTTGAACCCAGCAACAAAATGCCATCAATTTGCTTGGTGGTGATccaaacgctaa                                                                  >  2:422518/1‑240 (MQ=255)                                                                                                                                                                                      gCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAAATGTAGGCAGCTCCCGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATAGATAAAgggt                                                             >  2:167850/1‑249 (MQ=255)                                                                                                                                                                                       cATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGttt                                                            <  1:308256/251‑1 (MQ=255)                                                                                                                                                                                          cgctTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGtttt                                                           <  2:297148/247‑1 (MQ=255)                                                                                                                                                                                            ctTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATCAATAATTTTCTGCATCAAATGCGGCGG‑TCCG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTTGTTCGCCATCACCATCGGCGGCAGAATACGCTGTTTCTCAATGCTGGCCTCAAACTGCCGACCTT‑AACCCAGCCACAAACTGCCATCAATTTGCTTGGTGATGATCACATCGATAAAAGTTTTTTcc                                                       >  2:212050/1‑251 (MQ=255)                                                                                                                                                                                            ctTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCTCCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGtttt                                                           <  1:160549/247‑1 (MQ=255)                                                                                                                                                                                            ctTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGtttt                                                           <  1:160163/247‑1 (MQ=255)                                                                                                                                                                                                  gACCTGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATGTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCAACGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACTGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATGTCCTTGGGGATGAGCTAATCAATAAAgggttgaacctgctga                                                 <  2:200611/251‑15 (MQ=255)                                                                                                                                                                                                    ccGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCAGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTG‑Att                                              <  1:39011/251‑1 (MQ=255)                                                                                                                                                                                                            aCAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCt                                                                                                                                          <  1:292212/153‑1 (MQ=255)                                                                                                                                                                                                            aCAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGGGCAAATTCGTTCGCCATCACCATTGGCGGCAGATTTCGGTGTTCCTCCATGCt                                                                                                                                          >  2:292212/1‑153 (MQ=255)                                                                                                                                                                                                             ccgcccaTCCGTTGTTGCCCTTGTTCATATAAATAATTTTCTGCATCACATGCGGCGG‑TCAG‑ATTGTAGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGTTTGGTGAGGATCAAATCGAGAAATTTTTTTTCCTGCTT‑ATTTTGATgtgc                                     <  2:308160/245‑4 (MQ=255)                                                                                                                                                                                                                     ccGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAACTGCGGCGG‑TCCG‑ATTTTCGATATGAACTGTAGGCAGCTTCAGCTCCGGTGCAAATTCGGTCGCCATCACCCTCGGCGGCCGATTACGCTGTTCATCAATGCTGGCCACAAACGGCAG‑CCTTGAACCCCGCAAAAACATGCCATCAATTTTCTTGGGGATGATCCAATCGATAAAGGTTTTTTCCTGCTG‑ATTTTGTTGAGAACAGTCGc                             >  2:248306/1‑251 (MQ=255)                                                                                                                                                                                                                       gTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCCCCTCCAGATCCGGTGCCAATTCGTTCGCCATCACCATCGGCGGCAGCTTACGCCGTTCCTCAATGCTGGGCTCAAACGGCAG‑CCTTGTACCCAGCAACAACATGCCATCAATTTGCTTGGTGCTGATCCAATCGATAAAGGTTTTTTCCTGCTG‑ATTTTGATGCGCCCAGTCGcc                            >  1:170161/1‑250 (MQ=255)                                                                                                                                                                                                                               ccTTGCTCATCTAAATAATTTTATGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGACCTGTAAGCAGCTCCCGCTCCGGCGCCAATTCGTTCGCCATAACCCTCGGCGGCCGATTAAGCTGTTCCTCAAAGCTGGCCTAAAACGGCAG‑CCCTGCAACCAGCAACAACCTGCCATCAATTTTCTTGGTGATAATTAAATTGCTAAAGGATTTTTCCTGCTG‑Cttttttggcggccaatatccacacagcac                   >  2:185136/1‑226 (MQ=255)                                                                                                                                                                                                                                  tGCTCCTATAAATAATTTTCTGCATCAAATGCGGCGG‑CCAG‑ATTGTCGATATTAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGGGGCCGCTTCCTCTGGTCCTCAATGCTGGCCACAAACGGCCG‑GCTTGTACCCAGCTACAACCTGCCAACAATTTGTTTGGAGATGATCAAGTAGATAACGGTTTTTTCCTTCTG‑ATTTTGCTGCGCTCCGTCGCCCATCAGAAcccg                >  2:337835/1‑249 (MQ=255)                                                                                                                                                                                                                                      cATATAAATAATTTTCTGCATCCAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGTCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTG‑ATTTTGATGCGCACAGTCGCCAATCAGCACCAGat              <  2:312450/249‑1 (MQ=255)                                                                                                                                                                                                                                      cATATAAATAATTTTATGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCCCCAGCTCCGGTGCAAATTTGTTAGCCATCACCATCGGCGGCCGAATACGCCGTTCCTCAATGCTGGCATCCAAAGGCAG‑CCCTTAACCCCGCAACCACATGCCATCAATTTTCTTGGGTATGTACAAAATGATAAAGGCTTTTTCCCGATGTATTTT‑ATGGGCACCGTCGGCAATCAGCCCCAGatat            >  2:77135/1‑251 (MQ=255)                                                                                                                                                                                                                                                atTTTCTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTAGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTG‑ATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGAtttt  >  1:171021/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                           GCCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGG‑TCAG‑ATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAG‑CCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTG‑ATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTG  >  NC_000913/4123687‑4124176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑