Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	421,256	A→G	T91A (ACC→GCC)	proY →	putative transporter ProY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	421,256	0	A	G	100.0%	173.1 / NA	53	T91A (ACC→GCC)	proY	putative transporter ProY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/27);  total (26/27)

GGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCAT  >  NC_000913/421014‑421497                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  ggctagtaccccGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTgg                                                                                                                                                                                                                                           <  1:422549/242‑1 (MQ=255)         aCCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCAACGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGAGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTg                                                                                                                                                                                                                                   >  1:372192/1‑251 (MQ=255)           ccGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTTTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCt                                                                                                                                                                                                                                 <  1:26678/251‑1 (MQ=255)            cGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCtt                                                                                                                                                                                                                                <  2:149605/251‑1 (MQ=255)                acaTACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCGCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGGCCTACTGCTTTGa                                                                                                                                                                                                                             >  2:368724/1‑250 (MQ=255)                         ttATGGCACTGGGTTCAGCAAGTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTAGCGAGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGGCCTACTGCTTTGAAATCCTTAt                                                                                                                                                                                                                    <  1:342611/250‑1 (MQ=255)                          tATGGCACTGGGTTCCGCGCTTGGCACCGGGCTGTTTTACGGTTCGGAGGACGACATCAAAATGGCCGTTCCGGGCGTGCTGTTGGCCTATATTATCGGTGGTATCGCGGCGTCTATCATTCTGCGTGCGCTGGGGGAACTGTCGGTACTTAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGACAGGGTACATTGCCGGCTGGACCTACTGCTTTGCAACCTTTATTg                                                                                                                                                                                                                  >  2:423910/1‑251 (MQ=255)                          tATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTGCGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTAtt                                                                                                                                                                                                                   <  2:421226/250‑1 (MQ=255)                                         aGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAACATGGCCGGTCCGAGAGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGAGTGCGCTGGGGGAAATGTAGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGATTTGAAATCCTTATTGTCGCCATCGCCGAtg                                                                                                                                                                                                   >  1:395305/1‑251 (MQ=255)                                          gCAATTGGCCCCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGAtgt                                                                                                                                                                                                  <  2:315135/251‑1 (MQ=255)                                                    ccGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCtttt                                                                                                                                                                                        >  2:34083/1‑251 (MQ=255)                                                                      cGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATGATCGGTGGTATCGCGGCGTATATCATTATGCTTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGTTTTGAAATCCTTATTGTCTCCATCGCCGATTTGACCGCTTTTGGTATCTATATGGGTGTc                                                                                                                                                                      <  2:140202/251‑1 (MQ=255)                                                                        gCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATCTCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTg                                                                                                                                                                    >  1:171783/1‑251 (MQ=255)                                                                         cAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTCGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTgg                                                                                                                                                                   <  1:6704/251‑1 (MQ=255)                                                                            aCGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCTCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCAGCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTc                                                                                                                                                                <  1:390654/251‑1 (MQ=255)                                                                                       aTGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATACTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGcc                                                                                                                                                     >  1:281407/1‑251 (MQ=255)                                                                                         ggCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCg                                                                                                                                                    <  1:269565/250‑1 (MQ=255)                                                                                                            ttgGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCTTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTAGGCGGAGGAAAACCTCGGCCCGCTTGCAGGTTACATTGTCGGCTGGACCTACTGCTTTGAAATCCTTATGGTCGCCATCGGCGAGGTGTCCGCTTTTGGTAGCTTTATGGGTGTGTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGa                                                                                                                                 <  1:176813/250‑1 (MQ=255)                                                                                                            ttgGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGGATCTATATTGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTg                                                                                                                                  >  2:160937/1‑249 (MQ=255)                                                                                                               gccgatagtatCTGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATGGTCGCCATCGCCGATGTGTCCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTTCCGCACTGGATTTGGGTACTGAGCgt                                                                                                                             <  2:126013/243‑1 (MQ=255)                                                                                                                 cTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCCCTGGATTTGGGTACTGAGCgtgg                                                                                                                           >  2:56678/1‑251 (MQ=255)                                                                                                                      ttatCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGCTTTGGGTACTGAGCGTGGTGCTg                                                                                                                      >  2:2250/1‑251 (MQ=255)                                                                                                                              ggtATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTg                                                                                                              >  2:360610/1‑251 (MQ=255)                                                                                                                                tATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGCACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGCACTGAGCGTGGTGCTGATCCTTTgcg                                                                                                            >  2:432937/1‑251 (MQ=255)                                                                                                                                         gTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGTCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCTCCGATGTGACCGCGTGTGGTATCTATAGGGGTGGCTGGTTCCCGACGGTGCCGCACTGGAGTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAAcc                                                                                                   <  1:196769/251‑1 (MQ=255)                                                                                                                                            tatCACTATGCGTGCGCTGGGGGCAATGCCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACATCGGCCCGCTGGCCGGTTACATTGCCGGTTGGACCTACTGCTTTGCAATCATTATTGTCGCCATCGCCGCTGTGACAGCTTTTGGTATATATATGGGTGTCTGGTTCCCGCAGGTTCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCTCCGTAAACCtgg                                                                                                >  2:354649/1‑250 (MQ=255)                                                                                                                                             atCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGTCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCTCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCtgat                                                                                               <  1:251754/251‑1 (MQ=255)                                                                                                                                             atCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCtgat                                                                                               <  1:17838/251‑1 (MQ=255)                                                                                                                                             atCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCtga                                                                                                <  1:368091/250‑1 (MQ=255)                                                                                                                                               cATTATGCGTGCGCTGTTGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCGGGCCCTACTGCTTTGAAATCCTTGTTGTCGCCATCGCCGATGTTACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCCCTGGAATTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCtgatg                                                                                              >  1:93416/1‑250 (MQ=255)                                                                                                                                                             ggggggAAATTTCGGTACATACCCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCGATATGGGTGTCTGGTGCGCGACGGGGCCGCACTGGAGTTGGGTACTGAGCGTGGTGCTGATCATTGGCTCCGGAAACCTGAGGAGCGTGAAGGTATTc                                                                               <  2:409749/250‑1 (MQ=255)                                                                                                                                                              ggggataaggTCGGTACCTACCCCGGCCGCTGGCTCTTGCTCGCGTTATGCGCAGGAAAGCCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATATATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGTGCGTGGTGCTGATCATGTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCg                                                                              <  2:288029/242‑1 (MQ=255)                                                                                                                                                                gggcaaggacGGTACATAACCCGGCCGCCTGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTTTTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGGTCCCGACGGTGCCTCACGGGATTTGGGTACTGAGCGTGGTGCTTATCATGTGCGCCGTAAACCTTATGAGCGTGAAGGTATTCGGt                                                                            <  2:398475/242‑1 (MQ=255)                                                                                                                                                                    aaTGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACGTTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGGCGCCATCGCCGATGCAACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGTCGGTGCCGCACTGGATTTGGGCACTGAGCGTGGTGCTGATCCTTTTCGCCGTAAACCTGATGAGCGTGCAGGTATTCGGttcgt                                                                        >  2:84257/1‑247 (MQ=255)                                                                                                                                                                         cGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGGGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCACGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGGGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGAATTCGGGGAGCTGGa                                                                    >  2:298618/1‑250 (MQ=255)                                                                                                                                                                               aTAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAACCCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGAGTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTgg                                                             <  2:171783/251‑1 (MQ=255)                                                                                                                                                                               aTAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGAGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTgg                                                             >  1:171854/1‑251 (MQ=255)                                                                                                                                                                                 aaCCCGACCGCCAGCCCTTTCTCGCGTTATGCGAAGGACAACCTCAGCCCGCTGCCAGGTTACATTGCCGGCTGGCCCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTCGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAGGGTATTCGGTGAGCTGGAATTCTGGtt                                                           >  2:385137/1‑251 (MQ=255)                                                                                                                                                                                   cccGTCCGCCAGCTCGTTCTCGCGTTAGGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTtct                                                         <  2:148566/251‑1 (MQ=255)                                                                                                                                                                                       gccgccAGCTCTTTCCCGCGTGAGGCGCAGGAAAACCCCGGCCCGCTGGCCAGTTACATTGCCGTCGGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTTTCTATATGGGTGTCTGTTTCCCTACGGTGCCGCACTGGATTTTGGTACTGATCGTGGTGCTGATCAGTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCTGTGAGCTGGAATTCTGGTTCTCGtt                                                     <  2:264971/251‑1 (MQ=255)                                                                                                                                                                                                tctTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACATACTGCTTTGAAATCCTTATTGTCGCCATCGCAGATGTGACCGCTTTTGGCATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGTTCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGt                                            >  1:185323/1‑251 (MQ=255)                                                                                                                                                                                                   ttCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGt                                            <  1:160937/248‑1 (MQ=255)                                                                                                                                                                                                       cgcgTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGCTGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGccacc                                     >  1:190768/1‑251 (MQ=255)                                                                                                                                                                                                             aTGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACcatcatc                               >  1:235548/1‑251 (MQ=255)                                                                                                                                                                                                                     gAAAACCTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGAt                       <  1:56678/251‑1 (MQ=255)                                                                                                                                                                                                                           cTCGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGACATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGc                 >  2:179159/1‑251 (MQ=255)                                                                                                                                                                                                                             cGGCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACGGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGGCCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCAATTGCGCCGTAAACCTGATGAGCGTGAAGGTAGTCGGTGAGCTGGAATTCTGGTTCTCGGTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGc                 <  1:423910/249‑1 (MQ=255)                                                                                                                                                                                                                               gCCCGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGGGCTGATCATTTGCGCCGTAACCCtgtgg                                                                                              >  1:156624/1‑167 (MQ=255)                                                                                                                                                                                                                                  cGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTc          <  1:2250/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTc          >  1:260167/1‑251 (MQ=255)                                                                                                                                                                                                                                   gCTGGCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCAACATCGCCGATGTGACCGCTTTTAGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCg         >  1:196807/1‑251 (MQ=255)                                                                                                                                                                                                                                      ggCAGGTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGc       <  1:200582/250‑1 (MQ=255)                                                                                                                                                                                                                                          ggTTACATTGCCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGCCCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCAACCATCATCATCATGATTTTCGCCGGTTTCGGcatcat  >  2:354469/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  GGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCAT  >  NC_000913/421014‑421497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑