Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,922,994	T→C	intergenic (‑554/+63)	atpI ← / ← rsmG	ATP synthase accessory factor AtpI/16S rRNA m(7)G527 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,922,994	0	T	C	100.0%	97.8 / NA	29	intergenic (‑554/+63)	atpI/rsmG	ATP synthase accessory factor AtpI/16S rRNA m(7)G527 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/11);  total (18/11)

CCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTCACCAGGAAGATGG  >  NC_000913/3922749‑3923225                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        ccTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGaa                                                                                                                                                                                                                                     >  2:103360/1‑250 (MQ=255)       cAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTGTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCTCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTgg                                                                                                                                                                                                                              <  1:189989/251‑1 (MQ=255)                     ttCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGGTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGGGAACGTGGCGTTAAATGTATCCAGGTATATCAGCAGAAAACCTAGTTGTTGTTAACAg                                                                                                                                                                                                                 <  2:170628/250‑1 (MQ=255)                               aaaCAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGGCTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTTATGCAAAAGAGTGAACGTGGCGTTAAAGGTAGCCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTc                                                                                                                                                                                                      <  1:188208/251‑1 (MQ=255)                               aaaCAGATACGGTTTTAATAAATGAAATTTACACAACAAAAACCCCCCATTGACATTTTTAATAATGTTTTAACAGCCAATGAGGGTTCTGAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACGTATTTTCGGCTGACCGAAAAAAGGCACGCTATCTTGGTGATGCAAAAGAGTGAACGTGGGGTTAAATGTATGCAGTTATATCAGCAGAAAACCTTGTTGTTGTGAAGAGTGTGACCGGTc                                                                                                                                                                                                      <  2:21099/251‑1 (MQ=255)                                   aGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCTGTTATATCATCAGAAAACCTGTTTGTTGTTAACAGTCTAACCGTTCAAtt                                                                                                                                                                                                  <  2:373116/251‑1 (MQ=255)                                                         aTTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATaaaa                                                                                                                                                                            <  1:320426/251‑1 (MQ=255)                                                           ttACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATaaaaa                                                                                                                                                                           <  1:234739/250‑1 (MQ=255)                                                                 aacaaAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAaaattaaatt                                                                                                                                                                    >  1:427235/1‑251 (MQ=255)                                                                         ccgcccATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGc                                                                                                                                                            <  2:234750/248‑1 (MQ=255)                                                                                   aCATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTACTGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATcacc                                                                                                                                                  >  2:192919/1‑251 (MQ=255)                                                                                                 gTTTTAACAGCCAATGATGGATCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTc                                                                                                                                    >  1:136066/1‑251 (MQ=255)                                                                                                             aaTGATGGTTCTTAGCGCCGATTTTTAGCAGACTGACATTTTCACTAATGACTTATTTTCAGCTTACCCACAAAACACACGTTCTCTTGTCGATGCCACAGAGTGAACGTGGCGTTAAATGTAACCCGTTATATCAGCAGAAAACCCGGTTCTTGTTACCAGTCTATCCGGTCAATTTTTTATGATATTTTTGATACAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCCGGGc                                                                                                                        >  1:275966/1‑251 (MQ=255)                                                                                                                gatgGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTCTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTgg                                                                                                                     >  1:103212/1‑251 (MQ=255)                                                                                                                   ggTTCTTAGCGCCGATTTTTAGCAGCCTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAAc                                                                                                                  >  2:412986/1‑251 (MQ=255)                                                                                                                                          aGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGa                                                                                           <  2:244734/251‑1 (MQ=255)                                                                                                                                           gACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGACACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGCACCTGAAGTTTAACCACTGATTCGAc                                                                                          >  2:186881/1‑251 (MQ=255)                                                                                                                                                  atTTTCACTAATGACTGATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTGGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGatat                                                                                   <  2:332114/251‑1 (MQ=255)                                                                                                                                                    tttCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATAttc                                                                                 >  2:260270/1‑251 (MQ=255)                                                                                                                                                    tttCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATAtt                                                                                  <  2:273241/250‑1 (MQ=255)                                                                                                                                                     ttCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTCACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATAttct                                                                                >  2:346111/1‑251 (MQ=255)                                                                                                                                                     ttCACTAATGACTTAGTTTCTGCTTACCAAAAAAAGCCAAGTTATCTCGTTGATGCAAAAGAGTGAACGTGGCGTTAACTGTACACAGTTACATCAGCAGAAAACCTGGTTGTTGTTAACAGAACACCCGGTAAATTTTTTATGCTTTTTTTGATAAAAATTAAATTTTATTTGCGTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGCAGTTTAACCACTGATTCGACCTGATAttct                                                                                >  1:278868/1‑251 (MQ=255)                                                                                                                                                            aTGACTTATTTTATGCTTACCAAAAAACGCCACGTTATCTTGTTGATGCAAAAGACTAAACGTGGCGTTAACTGTATACAGTTATCTCAGCACAAAACCTGGTGGTTGTTAACCGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCCGGGCTGGACCCTGAATTTTAACCACTGATTCGACCTGATATTCTTCggcc                                                                          >  2:224880/1‑248 (MQ=255)                                                                                                                                                              gACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCTATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGcaac                                                                       >  2:83086/1‑251 (MQ=255)                                                                                                                                                                                       aGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCAttt                                              >  2:313518/1‑251 (MQ=255)                                                                                                                                                                                       aGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTGAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTAGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCACCAAAGCGATTTCATCTTACGGCAttt                                              >  2:2462/1‑251 (MQ=255)                                                                                                                                                                                                     gttgATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCAGGCATTTGCCCTTTCAGcgcg                                >  2:8138/1‑251 (MQ=255)                                                                                                                                                                                                          tGCAAAAGAGTGAACGTGGCGTTAAATGTACCCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCCTTTGCCCTTTCAGCGCGTAGa                            >  2:52093/1‑250 (MQ=255)                                                                                                                                                                                                                                   aTGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTCACCAGGAAGAtgg  >  1:13894/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        CCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTCACCAGGAAGATGG  >  NC_000913/3922749‑3923225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑