Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,393	G→A	intergenic (‑75/+20)	glyQ ← / ← ysaB	glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,393	0	G	A	100.0%	154.7 / NA	42	intergenic (‑75/+20)	glyQ/ysaB	glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/23);  total (19/23)

AGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTT  >  NC_000913/3725151‑3725637                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     agcCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACggg                                                                                                                                                                                                                                               >  1:77417/1‑250 (MQ=255) agcCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGa                                                                                                                                                                                                                                              <  2:161061/251‑1 (MQ=255)        aTCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAAGCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCa                                                                                                                                                                                                                                       <  1:381111/251‑1 (MQ=255)          cGGTTCTGGCCCCAGCTCGCGCGGACAGGTCGTTGGGTGAGAGGTTCCCGCGCCGTCTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAGTCCTGTAAGGTCAGGAGCAAGCCCGGGAAGGTCCTGGTATCAAACTTTTGCATATTAATTCGTGCTGGATACGTGGGGATTTAAAGGAAGGGATCAGTACACCCGCTGGATGGAAGATATACAGTACAAAACGTGAAAAAGCAg                                                                                                                                                                                                                                      <  2:161503/250‑1 (MQ=255)          cGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGTAAAAAGCAg                                                                                                                                                                                                                                      <  1:79817/250‑1 (MQ=255)                 ggCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAAc                                                                                                                                                                                                                              >  1:79747/1‑251 (MQ=255)                                   cAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATTAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAATATATGCAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaaaa                                                                                                                                                                                                            <  2:388049/251‑1 (MQ=255)                                      gTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAAt                                                                                                                                                                                                         <  1:368329/251‑1 (MQ=255)                                            gggTGAGAGGTTCCCGCGCCGACGTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCAt                                                                                                                                                                                                   <  2:239692/251‑1 (MQ=255)                                                gagaGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCcgc                                                                                                                                                                                               <  1:99462/251‑1 (MQ=255)                                                 agagGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCcgcg                                                                                                                                                                                              >  1:425168/1‑251 (MQ=255)                                                        cccGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAg                                                                                                                                                                                       <  1:212082/251‑1 (MQ=255)                                                                gACTTCCGTGTCCGAGGGTTGAACATTGATGCACCCGTGTCGAGACCAGTAATACTGAACGGTCACGCCCAAGCCATGGAAGGCCCTGGAACAAAACATAAGCATATTATTTAGTGCTGGATACGTGTGGATATAACGGCAGGAATCAGTATACCCGCTGGATGGAAGATATAAAGTACAACACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCAGCGTCAAAAGCACAGAc                                                                                                                                                                                >  2:24162/1‑250 (MQ=255)                                                                         gTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGctc                                                                                                                                                                       <  1:74876/250‑1 (MQ=255)                                                                                       cAATGGTGCAACCCCGGAAAGCCCAGTCATACTGTAAGATCCGGATAAAGACCTGGACGGTCCTGGTATCCAAATTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTCAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGACAAAGCAGGGATTAACGCATGGAAAGATGCAAAAAATGGCCATCACCGTCAAAAGAACAGACAACCTGCTCTTTACGGAAGGtat                                                                                                                                                         >  2:391554/1‑250 (MQ=255)                                                                                            gTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTTCTCTTTACGGAAGGTATAACcgc                                                                                                                                                   <  1:152036/251‑1 (MQ=255)                                                                                            gTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACcgc                                                                                                                                                   <  2:85508/251‑1 (MQ=255)                                                                                              gCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGGATAACCgcgc                                                                                                                                                 >  2:359567/1‑251 (MQ=255)                                                                                                aGCCCTGGCGAGCCCAGTAATCCTGGAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGGGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGGATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCAt                                                                                                                                               >  1:229628/1‑251 (MQ=255)                                                                                                     tGGCGCGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAAAAGACAAACTGCTCTTTACGGAAGGGATAACCGCGCATTTCAt                                                                                                                                          >  2:358290/1‑251 (MQ=255)                                                                                                                  aaTCCTGTAAGGTCATGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTAGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGCAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAACTGGCTAACCGCGTCAAAAGAACAGAAAAACTGCTCATTACGGAATGTATAACGGCGCATTTCATAGCTTCCCTgta                                                                                                                              >  2:246297/1‑248 (MQ=255)                                                                                                                        gTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATAGTATTGCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGGTATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGGAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGt                                                                                                                         <  2:241636/249‑1 (MQ=255)                                                                                                                                         cccTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCg                                                                                                       >  1:301281/1‑250 (MQ=255)                                                                                                                                            tGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGtata                                                                                                                                                        >  1:20419/1‑198 (MQ=255)                                                                                                                                            tGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGtata                                                                                                                                                        <  2:20419/198‑1 (MQ=255)                                                                                                                                                        gTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGAGTGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGGTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCgg                                                                                       <  2:340591/251‑1 (MQ=255)                                                                                                                                                          aTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATTGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTGACGGAAGGTATAACCGCTCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGAGGACGTCGATTTTTTGCTCGCCGgtg                                                                                     <  2:289582/251‑1 (MQ=255)                                                                                                                                                             acaCTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAAAGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACCAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGtt                                                                                   >  2:302353/3‑250 (MQ=255)                                                                                                                                                                      cATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGtttt                                                                         >  1:148011/1‑251 (MQ=255)                                                                                                                                                                                 gTGCTGGATCCGTGTGGATTTAAAGGAAGGCATCAGTATACCAGCTGGATGGAACATATACAGTACAAAACGGGCAAAAGCCGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCCTAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATc                                                              >  2:396914/1‑251 (MQ=255)                                                                                                                                                                                   gCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCt                                                            <  1:311630/251‑1 (MQ=255)                                                                                                                                                                                           cGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGAAATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAGCTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAAc                                                    <  2:77417/251‑1 (MQ=255)                                                                                                                                                                                                      aaaGGCAGGGATCAGTATCCAAGCTGGATGGAAGATATACAGTACAACAAGGGAACACGCAGGGCCTAACGCCTGGAAAGATGATAAAAATGGACATCCGAGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATACCCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAACGCGGAGACGTCGATTTCTTGATCGCCGGTGTTATAAAGTTTTGCAGCATGATCCTTGCCTaaagcaccggcctc                                         >  2:253903/1‑239 (MQ=255)                                                                                                                                                                                                      aaaGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGGCTGATCCTTGGATAACGCagcggcctc                                         <  1:141881‑M1/251‑10 (MQ=255)                                                                                                                                                                                                          gaaggCATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCAATCCGCGTCACAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggat                                     >  2:395619‑M1/1‑238 (MQ=255)                                                                                                                                                                                                                    gTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATc                                                              <  2:295718/216‑1 (MQ=255)                                                                                                                                                                                                                    gTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATc                                                              >  1:295718/1‑216 (MQ=255)                                                                                                                                                                                                                      ataCCCGGTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatca                         <  2:147459‑M1/251‑26 (MQ=255)                                                                                                                                                                                                                      ataCCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatc                          <  1:396914‑M1/250‑25 (MQ=255)                                                                                                                                                                                                                            gCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttt                   >  2:330002‑M1/1‑220 (MQ=255)                                                                                                                                                                                                                                   ggaAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttg             <  2:142024‑M1/250‑38 (MQ=255)                                                                                                                                                                                                                                              aGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagcc  >  1:230699‑M1/1‑202 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     AGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTT  >  NC_000913/3725151‑3725637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑