Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,718,336	A→C	*237E (TAA→GAA)	yiaF ←	DUF3053 domain‑containing protein YiaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,718,336	0	A	C	100.0%	42.1 / NA	15	*237E (TAA→GAA)	yiaF	DUF3053 domain‑containing protein YiaF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/7);  total (8/7)

GACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑CCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATCAACGGTTGCAATGCATCAGCTGGCGTCGTTACCACT  >  NC_000913/3718290‑3718580                                               |                                                                                                                                                                                                                                                      aagaaaatgccgatttgcggccgcaTCGCTCGGGCTTTTATTATTTCTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTACACGCCTGATGCTGTG‑CCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGCAGTCGGGAACTGGATGCCATTTGCGCCAAAGCTCACCTGAGTACCTTTCTGAGCAATGTAGTCTCCAACCATCACCAGTTTTTGCGTGAAAGTTTGTGCCGCCgg                                          >  1:206400/25‑251 (MQ=255)     aaatgccgctttgcggccgcaTCGCTCGGGCTTTTACTCTTTCTTGGGTTGCAGTCACTGCTGTAGTCCAGGCCTGATTCAACGCCTGATGCTGTG‑CCGGTACTGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGCCAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATc                                      >  2:272331/21‑251 (MQ=255)       atgccgatttgcggccgcaTCGCGCGGGCTTTTACTCTGTCTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑CCGGTAATGGCTCAAGCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTTGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGGTGCGTGAAATTTTGTGCCGCCGGAATCag                                    <  2:169370/233‑2 (MQ=255)         gccgatttgcggccgcaTCGCTCGGGCTTTTACTCTTTCTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑ACGGTACTGGCGCAATCCGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGCGTACCTTGCTGAGCAATGTAGTCTCCAACCTTCCCCAGTTGTTGCGTGACAGTTTGTGCCGCCGGAAtacacg                                  >  2:40302/17‑246 (MQ=255)             atttgcggccgcaTCGCTCGGGCTTTTACTCTTTCTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCT‑TGTCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATGTGCTACAAAGCTCACCTGAGTACCGTGCTGAGCAGTGTAGTCTCGATCCATCGCCAGTTGTTGCGTGAAATTTTGTGCCGCCGGAATCAACGGTTg                              <  1:210186/239‑1 (MQ=255)                         aTCGCTCGGGCTTTTACTCTTTCTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑CCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATCAACGGTTGCAATGCATCAGc                  >  2:93439/1‑251 (MQ=255)                           cGCTCGGGCTTTTACTCTTTCTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑CCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATCAACGGTTGCAATGCATCAGCt                 <  2:100123/250‑1 (MQ=255)                           cGCTCGGGCTTTTACTCTTTCTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑CCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATCAACGGTTGCAATGCATCAGCTg                <  1:339203/251‑1 (MQ=255)                            gCTCGGGTTTTTACTATTTCTTGGGTTGCAGTCACTGCTGTAGTACAGGCATGATTAAACACCTGATGCTGTG‑CCGGTAATGGCGCAGTCAGTTTGTTATATTCACTTGCCTGCTGTGAACTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATCAACGGTTGCAATGCATCAGCTgg               >  2:155696/1‑251 (MQ=255)                             cTCGGGCTTTTACTCTTTCTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑CCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATCAACGGTTGCAATGCATCAGCTGGc              >  1:337092/1‑251 (MQ=255)                                gggCTTTTACTCTTTCTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑CCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCTGGAATCAACGGTTTCAATGCATCAGCTGGcgtc           <  2:426138/251‑1 (MQ=255)                                 gggttttACTCTTGCTTGGTTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑CCGGGAATGGCGCAATCAGTTGGGTATATTCGCTTGTCTGCTGTGAAGTCGGGAACTGGATTCCATTCGCGACAAAGCTCACCTGAGTACCGTGCTGAGCAATGTAGTCTCGAACCATCGTCAGGGGTTGCGTTAAATTGGGTGCCGCCTGAATCAACGGGCGCATTGCATCAGCttacgtcg          <  2:391731/248‑8 (MQ=255)                                   cTTTTACTCTTTCTTGGGTTGCAGTAACTGCTGTAGTCGAGGACTGATTAAACGCCTGATGACGTG‑CCGGCAATGGCGAAAACAGATTGTTATCTTCCCTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATATGCGACAAAGCTCACCTGAGTACCTTGCTCAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATCAACGGTTGCAATGCATCAGCTGGCGTCGtt        >  1:409327/1‑251 (MQ=255)                                    ttttACTCTTTCTTAGGATGCAGTCACTGATGCAGTAAAGGTCTGCTTAAACGCGTGATGCTGTG‑CCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCATGCTGTGAAGTAGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCCACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATCAACGGTTGCAATGCATCAGCTGGCGTCGtt        >  2:204322/1‑250 (MQ=255)                                           ctTTCTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑CCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATCAACGGTTGCAATGCATCAGCTGGCGTCGTTACCACt  <  1:15889/249‑1 (MQ=255)                                               |                                                                                                                                                                                                                                                      GACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTG‑CCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCACCAGTTGTTGCGTGAAAGTTTGTGCCGCCGGAATCAACGGTTGCAATGCATCAGCTGGCGTCGTTACCACT  >  NC_000913/3718290‑3718580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑