| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,523,800 | C→G | Y310* (TAC→TAG) | mrcA → | peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,523,800 | 0 | C | G | 99.2% | 448.8 / NA | 126 | Y310* (TAC→TAG) | mrcA | peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (70/55); minor base T (0/1); total (70/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
GGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCA > NC_000913/3523553‑3524042 | ggATGCTGGCTGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACACTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGAc > 1:120112/1‑251 (MQ=255)ggATGCTGGATGAAGGGTATATCCCCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGAc > 2:371277/1‑251 (MQ=255)ggATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGTCGGTACTTAATAACGTGCTGGACTAGGAc < 1:120443/251‑1 (MQ=255)ggATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCGCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAATTGCCTATGAAGACGGTTATCGCATTTACACCTCCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAAGCGGTACGTAATAACGTGCTGGCCTATGAc < 2:374890/251‑1 (MQ=255)ggATGCTGGATGAAGGGTAAATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTGCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGAc < 2:238450/251‑1 (MQ=255) gATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACa < 1:352736/251‑1 (MQ=255) tGCTGGATGAAGGGTATATCACCCAACACAAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGCGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTACTAACGTGCTGGACGAGGACATg > 2:131433/1‑251 (MQ=255) tGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGAACCGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATgcgc > 1:266760/1‑251 (MQ=255) aTGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCCCTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCAc > 1:419398/1‑251 (MQ=255) gAAGGGTATATCACCCAACCAAAGTTCGATCAGACAAGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGATTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTCTGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGGGCTGGACTAGGACATGGGCCACgg > 2:430376/1‑251 (MQ=255) gAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACgg > 2:78392/1‑251 (MQ=255) atagcACCAAACAACAGATCGATCCGACACGCTCTGATGCGGTTAACGCTAACTATCACGCGCCGGAGATTTCTTTCTCTTCGCCGTACCTGAGCGAAATGGTGCGCCTGGAGATGTATAACCGTTATTGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAGAGTGCAGCAGTCCGCGCAGCGGGCGGTTCGTAATAACGGGTTGGACTAGGACATGCGCCACGGCGATcgc < 2:386163/247‑1 (MQ=255) ccccAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTCACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCATGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCTCATTTACCCCACCATCACCCGCAGAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGccc < 2:127253/250‑1 (MQ=255) cccAACAACAGTTCGTTCAGACACGCACGGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTGCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGTAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTGACACCACCATCACCCGCAAAGTGCACCAGTCCGCGCAGCAGGCAGTACGGAATAACGTGTTGGACTAGGACTTGCGCCACGTCTATCGCGGCCCg < 2:68462/251‑1 (MQ=255) caacaaCAGCTCGATCAGACACGCCCTGCGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAACGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGc > 2:199120/1‑251 (MQ=255) cGATCAGACACGCACTGAGGCGATTAACGCTAACGATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCTCCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAAGGTGCtg < 2:287070/250‑1 (MQ=255) gATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCtgtg > 1:90310/1‑251 (MQ=255) gATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCCGACATTGCTTTCTCTGCGCCGTCCCTGCGCGAAATGGTGCGCAAGGAGATGCATAACCGTTATGGCGACAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCCAAGAGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCAACGGCTATCGCGGCCCGGCAAATGTGCtgtg > 1:428798/1‑251 (MQ=255) gATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGAGCCGGCGATTGCTTTCTCTGCGCCGTACCTGCGCGCACTGGTGCGCCAGGAGCTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTCCACCACCATCACCCGCAAAGTGCAGCCGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAACTGTGCtggg > 2:403306/1‑249 (MQ=255) aTCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGACATGGTGCGCCCGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTgg > 1:53820/1‑251 (MQ=255) aTCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTgg > 1:95228/1‑251 (MQ=255) cAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGa > 1:296381/1‑250 (MQ=255) cacGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTg < 1:128145/251‑1 (MQ=255) acGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGATATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTgg > 1:241609/1‑251 (MQ=255) gAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGt < 1:324872/250‑1 (MQ=255) gAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCGTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCTAAAGTGCCTATGAAGACGGTTATCGCATTTACTCCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGGACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCGATCGCGGCCCGGCAAATGTGCTGTGGAAAGGGGTCGAGTc < 2:402895/251‑1 (MQ=255) gAGGCGATTAACGCTAACTATAACGCGACGGAGATTCCTTTCTCTGCACCGAACCTGAGCGAAATGGCGCGCCAGGACATGTATAACCGCTCTGGAGAAAGTGCCTATGCAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGGAAGTGGGCGAGTc > 2:214938/1‑251 (MQ=255) gATTAACGCTCACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCCGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGGGCTGTGGAAAGTGGGCGTGTCGGCgg > 2:246036/1‑250 (MQ=255) aTTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACATGAGCGAAATGGTGAGCCAGGAGCTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCCGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGACAGTGGGCGAGTCGGCGTg > 1:406624/1‑251 (MQ=255) gCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaa < 1:158610/251‑1 (MQ=255) aaCTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGCTaaca > 1:21265/1‑250 (MQ=255) cACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGACATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCCGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGACTGTGGGCGCGTCGTCGTGGGagaccaacc > 2:117756/1‑239 (MQ=255) cACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaacacca > 1:250291/1‑244 (MQ=255) cACGCGCCGGAGAGTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGAt < 2:421803/251‑1 (MQ=255) cgcgCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGCAATGGTGCGCCAGGAGATGTATACCCGTTATGGCGAAAGTGCCTATGCAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGCAAGTGGGCGAGTCGGCGTGGGATAACAACAAGAtt > 1:377681/1‑250 (MQ=255) gagaTTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCTCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACACCAAGATAACCGATAc < 2:43048/251‑1 (MQ=255) aTTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCt > 1:175764/1‑251 (MQ=255) tGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTg > 1:220404/1‑250 (MQ=255) tttCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAgg > 1:326397/1‑251 (MQ=255) ttCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTTCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAAGAACGTGCTGGACTAGGACATGCGCCACTGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAg < 2:353571/249‑1 (MQ=255) cgcgtcgcCGTACCTGAGAGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGGCTATGAAGACGCTTATCGCATTGACACCCCCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACAGGCGCCACGGCTATCGCGGCCCGGGAAATGTGCTGTGGAAAGGGGGCGGGACGGCTTGGGATAACAACAAGATTACCGGTTCGCTGAAGgcg < 2:210333/245‑1 (MQ=255) cGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAAcc < 1:406201/251‑1 (MQ=255) gTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGAGCCTATGAAGACGGTTATCGCATTTACACCACCATCAACCGCAAAGTGCAGCAGGCCGTGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCTCGGCAAATGTGTTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACCATAAGATTACCGATACGCTGAAGGCGCGGCCAACCt > 2:305210/1‑251 (MQ=255) aCCTGAGCGAAATGGTGCGACAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTTCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCTCGGTTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGATTCGGCGTTGTATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTa < 1:34437/250‑1 (MQ=255) gAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACCAGATTACCGATACGCTGCAGGCGCTGCCCACCCATGGTc > 2:352654/1‑251 (MQ=255) gAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTc > 1:157581/1‑251 (MQ=255) aGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACAACACCATCACCCGCAAAGTGCAGCAGGCCGAGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTCTCGCGGCCCGGCAAATGTGCTGTGGACAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGAAACGCTGAAGGCGCTGCCAACCTATGGTcc > 2:250034/1‑251 (MQ=255) gAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCgct < 1:246036/251‑1 (MQ=255) gAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCgct > 1:302864/1‑251 (MQ=255) aaaTGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCgctg > 1:266605/1‑251 (MQ=255) tGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGctgctg > 1:336145/1‑251 (MQ=255) gcgcCAGGAAAGGTATAACCGTTAGGGCGAAAGTGCCTAGGAAGACGGTTAGCGCGTTTACACCACCATCGCCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGATCCGTAATAACGGGCTGTACGAGGGCAGTCGCCACGGCTAGCGCGGCCGGGCAAATGGGCGGTGGAATGTGGGCGAGTCAGGGTGGGATAACAACAAGATGACCGGTACTCCGAAGGCGGTGCGAGCCTATGTTCCGCTGatgcctg < 2:386303/251‑1 (MQ=255) gcCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGCCATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCtgcctgcc > 2:362113/1‑251 (MQ=255) gcCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCtgcctgcc > 1:230206/1‑251 (MQ=255) gagaTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTTGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTTTGGTCCGCTGCTGCCTGCCGCA‑Gt < 2:56422/251‑1 (MQ=255) ggaTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACTGTTATCGCATTTACACCACCATCACCCGCAAAGTTCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGGGGGATAACAACAAGATTACCGATGCGCTGAAGGGTCTGCCAGCCTATGGTCCGTTGCTTGCTGCCGCA‑GTc < 2:212441/250‑1 (MQ=255) aTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACTTATGGTCCGCTGCTGCCTGCCGCA‑GTCAc > 1:289706/1‑251 (MQ=255) gTATAACCGTTATGGCTAAAGTGCCTATGAAGACGGTTATCGCATTTACACCCCCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGTCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCAcc < 2:353717/250‑1 (MQ=255) gTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCAcc > 1:89610/1‑250 (MQ=255) aCCGTTATGGCGATAGTGCCTATGAAGACGGTTATCGCATTTAAACAACCATCACCCGCAAAGTGAAGCAGGCCCAGCAGCAGGCGGAACGTAATAACGTGCTGGACTAGCACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCCCCAGCGcc > 1:74993/1‑251 (MQ=255) gTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAAt > 1:332984/1‑251 (MQ=255) gTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCGGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAAt < 1:131433/251‑1 (MQ=255) ttATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCCCCAGCGCCAATc > 1:63681/1‑251 (MQ=255) ggCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCACAGTGCAGCAGGCAGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTc > 2:405351/1‑250 (MQ=255) ggCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTc < 1:362210/250‑1 (MQ=255) gCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAACGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGATGAAGGCGCGGCCAACCTATGGTCCGCGGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTccc > 2:239792/1‑249 (MQ=255) gAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCa < 1:309049/251‑1 (MQ=255) aaaGTGCCTATGGAGACGCTTAGCGCATTTACACCACCATCACCCGCAAAGCGCAGCAGGCCTCGCAGCAGGCGGTACGTAATAACGCGCTGGACTAGGCCATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGGTGGATAACAACAAGATGACCGAAACCCTTAGGGCTCTGCCAACCGATGTGCCGGTGCTGCCCGCC‑CACGTCACCAGCCGCAATCCTCCGCa > 2:64815/1‑250 (MQ=255) aaaGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCa > 2:108898/1‑250 (MQ=255) aaGTGCCTATGAAGACGGCTACCGCCTTTACACCACCCTCACCCGCACAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCacg > 2:378389/1‑249 (MQ=255) ccTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACg < 1:425637/251‑1 (MQ=255) aTGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGc < 2:95228/250‑1 (MQ=255) aaGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATGACCGATACGCTGAATGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATg < 1:214938/251‑1 (MQ=255) aCGGTTATCGCCTTTACACCCCCCTCACCCGCACAGTGCCGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGCAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTg > 2:22659/1‑251 (MQ=255) gTTATCGCATTTACACCCCCAGCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATACCGTGCTGGACTAGGACCTGCGCCCCGGCTATCGCTGCCCGGCAAAGGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCATCGCCAATCCTCAGCAAGCggctgcgagtctggcg < 2:189230/251‑16 (MQ=255) tATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGAAATAACGTGCTGGACTCGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCggg > 2:218067/1‑250 (MQ=255) aTCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGAc > 2:369219/1‑251 (MQ=255) ttACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCg < 2:406003/250‑1 (MQ=255) caaccaccATCAACCGAAAAGTGCAGAAGGCCTCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGATGGCGGACGGGTCGAcc < 2:104886/249‑1 (MQ=255) acacCACCATCACCCGCAAATTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACTTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGGGCTGTGGAAAGTGGGCGAGTCGGCGTGTGATAACAACAAGATTACCGATACGCTGAAGGCGCTTCTAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCTGCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGAcc < 1:55035/251‑1 (MQ=255) ccaccaTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCGGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTc > 1:102098/1‑251 (MQ=255) accaTCACCCGCAAGGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGc > 1:230421/1‑251 (MQ=255) cccGCAAAGTGCCGCAGGCCGCGCAGCAGTCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATGGCGGCCCGGCAAATGTGCTGTGGAAAGGGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAATGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCTACCGTCGCATTGAGt < 2:295832/251‑1 (MQ=255) cGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTCCGTAATAACGTGCTGGACTAGGACATGCGCCCCGGCTAGCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTAt < 2:204348/251‑1 (MQ=255) aaaGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGa < 2:296381/251‑1 (MQ=255) aaGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTAACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGTCGGGTCGCCCGTCGCCTTGAGTCTGGa > 2:361251/1‑250 (MQ=255) gcagcaGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGc < 1:295708/250‑1 (MQ=255) gcgcAGCGGGCGGTACGGAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGGGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGgc < 2:230206/251‑1 (MQ=255) cgcAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGgcg < 1:387450/251‑1 (MQ=255) agGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGTCGTTCGCTGGGCGCGTCCt < 2:90310/251‑1 (MQ=255) ggCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCtt < 1:403306/251‑1 (MQ=255) aCGTAAAAACGGGCTGGCCTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGtt < 2:318619/251‑1 (MQ=255) cGTAATATCGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGTAAAGTGGGCGAGTCGGCGTGGGAGAACAACAAGATGACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATTGAATGCGGTCGCTGGGCGCGGCCTTACCGTTc < 2:419398/251‑1 (MQ=255) cGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTc > 2:123432/1‑251 (MQ=255) ataaCGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGAt < 1:39232/251‑1 (MQ=255) agaaCGGGCTGGACTAGGACAGGCGCCGCGGCTATGGGGGCCCGGGAAAGGTGCGGCGGAAAGTGGGCGATTCCGCGGGGGATGACAACAAGATTACCGATACGCGGAAGGCGCTGCCAACCCCGGGTCCGCTGCTGCCTGCCGCC‑GTAACCAGCGCCAATCCTCAGCGAGCGACGGCGGTGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGAt < 2:2852/249‑1 (MQ=255) aCGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGGATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACt > 2:4810/1‑251 (MQ=255) cGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTc > 1:71117/1‑251 (MQ=255) cGTGCTGGAATAGGACATGCGCCACGGCTAACGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTc < 2:157581/251‑1 (MQ=255) ggACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGCAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCa > 1:221387/1‑249 (MQ=255) | GGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCA‑GTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCA > NC_000913/3523553‑3524042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |