Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,484,656	T→C	I56T (ATC→ACC)	prkB →	putative phosphoribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,484,656	0	T	C	100.0%	222.0 / NA	70	I56T (ATC→ACC)	prkB	putative phosphoribulokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/34);  total (36/34)

CGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGTTTACACGGCGGCGTAGT  >  NC_000913/3484414‑3484899                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    cgcgCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCaa                                                                                                                                                                                                                                               >  1:131924/1‑249 (MQ=255)      agtcagtTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTGTCACCGTTTCACCCGCCCGGAAATGGACATGGCGACCCGCAAAgcgcgc                                                                                                                                                                                                                                        <  1:188778/251‑1 (MQ=255)       gtcagtTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGCAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCCAAGcgcgcg                                                                                                                                                                                                                                       >  2:48116/1‑251 (MQ=255)                gagAATAACCTGCAACCGTCGGCGGACGAGATAAAAATGATAGCTAAGTCGGGGAGTTACTATGGCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTGACACCCGCCCGGAAATGGACATGGCGACCCGCTGATCGCGCGACGCCGggc                                                                                                                                                                                                                              <  2:287262/249‑1 (MQ=255)                  gAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGggcgg                                                                                                                                                                                                                            >  2:143724/1‑251 (MQ=255)                      aaCCTGCAACCGGCGGCGGACGAGAAAATAAGGAGAGCTAAGTCATGGAGTTACTATGTCGGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCat                                                                                                                                                                                                                         <  2:359838/250‑1 (MQ=255)                          tGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATc                                                                                                                                                                                                                      >  1:57054/1‑249 (MQ=255)                           gCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGCGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAACCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGc                                                                                                                                                                                                                   >  1:390034/1‑251 (MQ=255)                             aaCCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACACGCCCGGAAATGGACATGGCGACCCGCACAGCGCGCGACGCCGGGCGGCATATCAGCt                                                                                                                                                                                                                  >  1:165405/1‑250 (MQ=255)                                    ggcggACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCTGCATATCAGCTACTTCGGc                                                                                                                                                                                                          <  1:314481/251‑1 (MQ=255)                                           gagaTAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGCAACAGGATCCAGCGGCGCGGGGACCCCCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAgg                                                                                                                                                                                                   >  1:203382/1‑251 (MQ=255)                                             gaTAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCt                                                                                                                                                                                                 >  1:389620/1‑251 (MQ=255)                                                aataatGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTaa                                                                                                                                                                                               <  1:226601/250‑1 (MQ=255)                                                   aatGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACgcc                                                                                                                                                                                           >  2:212789/1‑249 (MQ=255)                                                   aatGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGAc                                                                                                                                                                                           >  1:225374/1‑251 (MQ=255)                                                        tAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCgg                                                                                                                                                                                      >  1:147605/1‑251 (MQ=255)                                                          gCTAAGTCATGGAGTTACTATGTCTGCCAACCATCCGGACATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACAAGCATCGCGTTTCGTAAAATCTTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGCAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGCGGCTAACGACTTCGGcc                                                                                                                                                                                    >  1:66794/1‑251 (MQ=255)                                                              aGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATAAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCctgct                                                                                                                                                                                >  1:15532/1‑251 (MQ=255)                                                                   tGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAAc                                                                                                                                                                           >  1:189930/1‑251 (MQ=255)                                                                         tactagggcTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAAGGACGTCGGCCTGCTGTATCAAAcc                                                                                                                                                                      <  2:195782/242‑1 (MQ=255)                                                                         tACTATGTCTGCTAAACATCCGGTAATTGCGGTAACAGGATCCAGTGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTACAGAATTCGGCCTGCTGGAACAAACCt                                                                                                                                                                     >  2:200187/1‑251 (MQ=255)                                                                          aCTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCtt                                                                                                                                                                    >  1:87902/1‑251 (MQ=255)                                                                               gTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTg                                                                                                                                                               >  1:182086/1‑251 (MQ=255)                                                                                        aCATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCGCCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGGTAAATCTGCAGGCAGCTGAGGTGGAAGGCGTCAGTTTTCACCGTTACACCCGCCCGGAAAGGGACAGGGCGGCCCGCAAAGCGCTCGACGGCGGGCTGGAGAGCAGCGACTTCGGCCCCGAGGCGAACGACTTCGGCCGGCTGTGACAAACCGTCATTGTACACGGTc                                                                                                                                                      <  2:146118/251‑1 (MQ=255)                                                                                        aCATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTc                                                                                                                                                      <  1:253023/251‑1 (MQ=255)                                                                                        aCATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGAACACCACCACCCGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGAGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGCCTTCGGCCTGCTGGAACAAACCTTCATTGAATACggcc                                                                                                                                                      >  1:419690/1‑249 (MQ=255)                                                                                          aTCCGGTCATTGCGGTAACAGGCTCAAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCACGCAGTTCAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACACGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGCATACGGTc                                                                                                                                                      >  2:404041/1‑249 (MQ=255)                                                                                           tCCGGTCATTGCGGTAACAGGCTCCAGCGGCGCGGGGACCACCACCACCCGCCTCGCGTTTCGTAAAATATTCGCGCCGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTc                                                                                                                                                      >  2:65140/1‑248 (MQ=255)                                                                                           accGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTGCACCGTTACGCCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCAGATCAGCTACTTCGGCCCCGAGGCTAACGACGTCGGCCTGCTGTAACAAACCGTCAGTGAATACGGTCa                                                                                                                                                     <  2:203382/248‑1 (MQ=255)                                                                                                 cATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGc                                                                                                                                              >  2:343321/1‑250 (MQ=255)                                                                                                  aTTGCGGCAACAGGATCCAGCTGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGGGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCaa                                                                                                                                            <  2:154549/251‑1 (MQ=255)                                                                                                     gCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCGGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAg                                                                                                                                          <  1:413136/250‑1 (MQ=255)                                                                                                        gTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGaa                                                                                                                                      <  2:87902/251‑1 (MQ=255)                                                                                                             aGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTCAAATCTGCATGCCGCCGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCCTATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAAtctc                                                                                                                                 <  1:258/251‑1 (MQ=255)                                                                                                              ggATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGCAATCTCg                                                                                                                                >  2:151947/1‑251 (MQ=255)                                                                                                                      cggcgCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATc                                                                                                                        >  1:138539/1‑251 (MQ=255)                                                                                                                            ggggACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATa                                                                                                                  <  2:131924/251‑1 (MQ=255)                                                                                                                            ggggACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAAGGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGAc                                                                                                                                                                                           <  1:63323/178‑1 (MQ=255)                                                                                                                            ggggACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCACGCAGTTAAATATGCATGCAGATGAGGTGAACGGCGACAGTTTTAACCGTTACACCCGCCCGGAAATGGACACGGAGACCCGCAACGCGCGCGCCGCCGGGCGGCATCTCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACATTCATTGCATACGGTCAGAGCGGCAACGGGCAATCTCGCAAATATCTGCAt                                                                                                                   >  1:191913/1‑250 (MQ=255)                                                                                                                              ggACCACCCCCACCAGCCTCGCTTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGATGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATAcc                                                                                                                <  2:219858/251‑1 (MQ=255)                                                                                                                                 ccaccacccccaGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACGTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAAGCGCGCAAATATCTGCATACCTac                                                                                                             <  2:390034/251‑1 (MQ=255)                                                                                                                                 ccaccaccaccaGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACACACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAACTATCTGCATACCTac                                                                                                             >  2:313623/1‑251 (MQ=255)                                                                                                                                   cccaccaccaGCATCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTacga                                                                                                           <  2:93648/250‑1 (MQ=255)                                                                                                                                    ccaccaccaGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTacgac                                                                                                          <  2:111659/251‑1 (MQ=255)                                                                                                                                    ccaccaccaGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTacgac                                                                                                          >  2:363791/1‑251 (MQ=255)                                                                                                                                      accaccaGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGGGGAAGGCGACAGTTGTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCGAACGACTGCGGCCTGGTTGAACAAACCTGCATTGAAGACGGTCAGGGCGGCGAAGGGAAATCTCGCAAATATCTGCATACCTAcgacga                                                                                                        <  1:215744/251‑1 (MQ=255)                                                                                                                                         accaGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGc                                                                                                     <  1:151947/251‑1 (MQ=255)                                                                                                                                          ccaGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAAGCTGCATGCGTCTGAGGGGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGTCGTCAGATCAGCTACTTCGGCCCCGAGGCTGACGACTTGGGCCGGCTGGAACAAATCTTCACTGAATACGGTCATTGCTGCAGAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGcc                                                                                                    <  1:23440/251‑1 (MQ=255)                                                                                                                                           caGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCg                                                                                                   <  1:69885/251‑1 (MQ=255)                                                                                                                                                tCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCg                                                                                              >  1:27231/1‑251 (MQ=255)                                                                                                                                                        cgaaaaaTAGTCGCGCAGTGAAAGCTGCATGCAGCAGAGGTGGAATGCGACAGTTTTCGCCGTTACACCCGCCCGGAAATGGACATGGCGCCCCGCAACGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGTCTAACGCCTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTCCGACGAAGCCGTACCGTGGaagc                                                                                       <  2:236224/247‑3 (MQ=255)                                                                                                                                                         gTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGTGGGAGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATc                                                                                       >  2:78208/1‑249 (MQ=255)                                                                                                                                                           aaaaTATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCTTGGAATCAgg                                                                                    <  1:109217/250‑1 (MQ=255)                                                                                                                                                            aaaTATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGGCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTa                                                                                  <  2:189930/251‑1 (MQ=255)                                                                                                                                                                    gcgcAGTTAAATCTGCATGCAGCTGAGGTGGAGGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGAc                                                                          <  1:78208/251‑1 (MQ=255)                                                                                                                                                                                  gcatgcaGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACTAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCt                                                               <  1:200187/248‑1 (MQ=255)                                                                                                                                                                                   catgcaGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGACCCGAGGCTAACGACTTCGGCATGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCACATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGCACCGGGGACATTAACCCCCTGGc                                                            >  2:363343/1‑250 (MQ=255)                                                                                                                                                                                            gAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTAc                                                   <  1:363791/250‑1 (MQ=255)                                                                                                                                                                                                 ggAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAAc                                             >  2:305906/1‑251 (MQ=255)                                                                                                                                                                                                       ccccatctttCACCGTTGCCCCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCTACGCCTGGCGGCATATCAGCTACGTCGTCCCCGAGGCTAACGACTTCCGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCAc                                         <  2:160342/242‑1 (MQ=255)                                                                                                                                                                                                         aCAGTTTTCCCCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGAt                                     <  2:96010/251‑1 (MQ=255)                                                                                                                                                                                                         aCAGTTTTCCACGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAACTCTCGCAAATATCTGCATACCTACGACGCAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTgtt                                     >  1:310499/1‑249 (MQ=255)                                                                                                                                                                                                           aGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGTCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGt                                   >  1:176934/1‑251 (MQ=255)                                                                                                                                                                                                               ttCACCGTTACACCCGCCCGGAAATGGCCATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCGACCTTTACCGGAACCCAc                                         >  1:160342/1‑241 (MQ=255)                                                                                                                                                                                                               ttCACCGTTACACCCGCCCGGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACt                                <  1:348075/250‑1 (MQ=255)                                                                                                                                                                                                                            ccgcccgGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGtt                  >  1:217083/1‑251 (MQ=255)                                                                                                                                                                                                                              gcccgGAAATGGACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTCCCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTAGGAAGGttt                 >  2:186149/1‑250 (MQ=255)                                                                                                                                                                                                                                        ggACATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCATGTACCGGGGACATTCACCCCCTGGCAACCTTTAGCGGAACCCACTGATGTACTGTTTTATGAAGGTTTACACggcggc       <  1:404041/250‑1 (MQ=255)                                                                                                                                                                                                                                           cATGGCGACCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGTTTACACGGCGGCGt     >  1:227256/1‑249 (MQ=255)                                                                                                                                                                                                                                            aTGGCGACCCGCAAAGCGCGCGACGCCGGGCGTCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTGCCTGAACCCACTGATGTACTGTTTTATGAAGGTTTACACGGCGGCGTAGt  <  1:52492/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    CGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAATGATAGCTAAGTCATGGAGTTACTATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAAAATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGTTTACACGGCGGCGTAGT  >  NC_000913/3484414‑3484899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑