Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,768	C→T	W362* (TGG→TGA)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,768	0	C	T	96.9%	214.3 / NA	65	W362* (TGG→TGA)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (38/24);  minor base G (0/2);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGT  >  NC_000913/3468524‑3469013                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                          cccATGCGTCTTGATAGACAGCAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCg                                                                                                                                                                                                                                                     >  1:337564/1‑251 (MQ=255)          cTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTa                                                                                                                                                                                                                                             >  1:310898/1‑250 (MQ=255)             gATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGGACGCTCGAGACGCAATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGGTCTTGTAAAc                                                                                                                                                                                                                                          >  1:247194/1‑250 (MQ=255)                  aCAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCGTGTAAACATTAcc                                                                                                                                                                                                                                    <  1:321868/251‑1 (MQ=255)                   cAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTAAGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCACTTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGCGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTAcc                                                                                                                                                                                                                                    >  1:189144/1‑250 (MQ=255)                    aGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCAt                                                                                                                                                                                                                                  <  1:315353/251‑1 (MQ=255)                      aaGTTTTATAATGTGCAGTGTTATAATCTACGATGCGGTCCGCGCTGTACGTTTGGCGGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTg                                                                                                                                                                                                                                <  2:319004/251‑1 (MQ=255)                       aGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCCCGCAGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACCTTACCATTg                                                                                                                                                                                                                                >  1:49046/1‑250 (MQ=255)                              gagtttgactCGTTATATACTACGAAGCAGTCCGCGCTAGAGGCTGTGCTTGCCTGGAAATCGTCCGCAGCAAGGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGGTACCGTACTGACTCAAGTCCGCAGCGGTAGCGGTACGCTCGAGGCGCCAGGGGTTATTGGAGTTGTCAGCGTTTGTGCCCCGTGTGCGAGCTTTAGAGCCAACGCATCAGGCTTTCTGGGTAACAGTACCATCGAAAATAAc                                                                                                                                                                                                                        <  2:194682/242‑1 (MQ=255)                              aaaggtgCACCGTTATAATCTACGATGCCGTACGCCTTTTACGCTTTGTTGTCCTGTAAACCATCCGCAACAAGGACGCCGCCGTTAGCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGCACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATGGAAAAGAAc                                                                                                                                                                                                                        <  2:322660/247‑1 (MQ=255)                                  ttgCATCGTTATAATCTACGATGCGATCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATg                                                                                                                                                                                                                     <  2:104515/249‑1 (MQ=255)                                      aTCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTaa                                                                                                                                                                                                                 >  1:341660/1‑250 (MQ=255)                                        cgtaataATCTACGATGCTGTCCGGGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑tt                                                                                                                                                                                                             <  1:13281/247‑1 (MQ=255)                                               aTCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGCCGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGTCCCAACACATCCGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTAc                                                                                                                                                                                                      >  2:151504/1‑251 (MQ=255)                                                   ccGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCt                                                                                                                                                                                                    <  2:341660/248‑1 (MQ=255)                                                     gATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTCCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑t                                                                                                                                                                                               >  2:305777/1‑251 (MQ=255)                                                      aTGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑Tg                                                                                                                                                                                              >  1:250240/1‑251 (MQ=255)                                                            tCCGCGCTGTCCGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACTCCGCCGGTATCAATTTCACAGGTAGTCGGTTTACCGTGCTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGTCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGTCAATCTTTAGACCCAACCCAGCAGGCGTTCTTGTAAACATTCCCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGc                                                                                                                                                                                         <  1:47298/250‑1 (MQ=255)                                                               gcgcTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTcc                                                                                                                                                                                      >  1:430362/1‑250 (MQ=255)                                                                cgtggTACGCTTTGCTGGCCGGGAAACCATCCGCAACAAGGACGCCGCCGTTATCAATTTCACAGGGAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAATCATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCAc                                                                                                                                                                                    <  1:221569/247‑1 (MQ=255)                                                                                     cgAAACCATCCGCCACAATGACGCCGCGGATATCACTTTCCCAGGAAGACGGGTTACCGAACTGACTCAATTCAGCCGCGATAGCTGTACGCCAGCGACGCCATGGGTTATTGGAGTTTTCTGCGTTGGTAACCCGTAGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTAAGACTAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCTGCTCCAGGCTTCCAATCAGTAATCGtt                                                                                                                                                                >  2:216682/2‑250 (MQ=255)                                                                                      gAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGACAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTa                                                                                                                                                               >  2:135278/1‑250 (MQ=255)                                                                                       aaaCCATCCGCAACAATGGCGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTTGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTTAAAATAACATGATAAA‑TTTCGCTTCCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAAc                                                                                                                                                             <  1:33105/251‑1 (MQ=255)                                                                                        aaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAAGTACACGGGTTAACGTACTGACTCAAATCCGCAGCGGTAACTGTACGATCGAGACGCCATGGGATATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCCTGTAAACATTACCATTGAACATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCAGCTCCAAGCTTGCAATGAGTAATCGCTAACg                                                                                                                                                            >  1:213788/1‑251 (MQ=255)                                                                                         aCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGc                                                                                                                                                           >  2:81327/1‑251 (MQ=255)                                                                                         aCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGc                                                                                                                                                           >  1:11298/1‑251 (MQ=255)                                                                                                gCAACACTGACGACTCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCAATGCGTTATTGGAGTTTTAAGCGCTGGGACCCCGTGGGCAATCGTTAGACCCAACCCATAAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGGATA‑TTTCGATCCCTTCC‑TGGACGCTCCACGCTTGCACTGCGTGATCGTTAAAGCTATCCGc                                                                                                                                                    >  2:184171/1‑251 (MQ=255)                                                                                                 caacaaTGCCGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTACTCGTTAACGCTATCCGCt                                                                                                                                                   >  2:365105/1‑251 (MQ=255)                                                                                                     aaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGgtg                                                                                                                                               >  1:15137/1‑251 (MQ=255)                                                                                                      aTGACGCCGCCTTTATCAAGTTCACAGGTAGTCGGGTTACCGTACTGACTCAATGCCGCAGCGGTAGCTGGACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTGTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCGACCTTCC‑GGGCCGCTCCACGCTTGCAATGAGGAATCGGTAACGCTATCCGCTGgtgt                                                                                                                                              <  2:414291/251‑1 (MQ=255)                                                                                                      aTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGgtgt                                                                                                                                              >  1:414291/1‑251 (MQ=255)                                                                                                        gACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTGCCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTg                                                                                                                                            <  2:412354/251‑1 (MQ=255)                                                                                                         ccgccgcCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGa                                                                                                                                           <  1:228170/250‑1 (MQ=255)                                                                                                         aCGCCGCCGTTATCAATTTCACAGGTAGTCGGGATACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGAGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTgg                                                                                                                                           >  2:323073/1‑250 (MQ=255)                                                                                                               ccgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCCCTTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTc                                                                                                                                     >  1:364224/1‑251 (MQ=255)                                                                                                                     ccAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGtt                                                                                                                               <  2:337564/250‑1 (MQ=255)                                                                                                                       aaTTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTAt                                                                                                                             >  2:379493/1‑251 (MQ=255)                                                                                                                                ggTAGTAGGGCTACCCTCCTGCCTCCATTCCGCACCGGTAGCTGTACGCTCGAGCCGCCATGGCTTTTTGGTGTTTTCATCGCTGGTACCCCGTGGGCAATCTTTAGACCCCACCCATCTGACGTTCTTGTCAACATTACCATTGAAAATTACAt                                                                                                                                                                                                                      >  2:381851/1‑155 (MQ=255)                                                                                                                                ggTAGGCGGGTTACCGTACTGACTCAATGCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTTAGCACTGGTACCCCGGGGGCAATCTTAAGACCCAACCCAGCAGGCGTTATTGTAAACATTACCATTGAAAATAACAt                                                                                                                                                                                                                      <  1:381851/155‑1 (MQ=255)                                                                                                                                        ggTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCAcc                                                                                                           <  1:243101/251‑1 (MQ=255)                                                                                                                                               gTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGttt                                                                                                    >  1:298862/1‑251 (MQ=255)                                                                                                                                                    gACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCGATCCGCGGGTGTTGATGGCGCGCTGTTATCCGGAGTTGGGT‑TCACCGGTGTTTCCg                                                                                                 <  2:430362/249‑1 (MQ=255)                                                                                                                                                        cAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGtt                                                                                            <  1:152924/250‑1 (MQ=255)                                                                                                                                                         aaTTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑t                                                                                                                                                                                                              >  2:145786/1‑137 (MQ=255)                                                                                                                                                         aaTTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑t                                                                                                                                                                                                              <  1:145786/137‑1 (MQ=255)                                                                                                                                                              cGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCaa                                                                                     <  1:240798/251‑1 (MQ=255)                                                                                                                                                                    ggTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCgg                                                                                 >  2:294611/1‑249 (MQ=255)                                                                                                                                                                             aCGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGAACCCCGTGGGCAATCTTTAGACCCAACCCATCCGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATAAACCGGAG‑TCACAGCAc                                                                     >  1:408486/1‑251 (MQ=255)                                                                                                                                                                                  cgagtggCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTCCCTTCC‑TGGCCGCTCCCCGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGAGGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCa                                                                <  2:364224/245‑1 (MQ=255)                                                                                                                                                                                  cGAGACTCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCATCCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTGCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGcc                                                                 <  2:334257/250‑1 (MQ=255)                                                                                                                                                                                     gaCGCCATGGGTTCTTGGAGTTTTCAGCGCTGGAACCCCGTGGGCAATCTTTAGACCCAAACCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGc                                                                                                                                                           >  2:413263/1‑159 (MQ=255)                                                                                                                                                                                     gaCGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGc                                                                                                                                                           <  1:413263/159‑1 (MQ=255)                                                                                                                                                                                                ttATTGGAGTATTAGGCGCTGGTAGCCCGTGGGTGATCTTTAGACCCAACCCCTCAGGCGCTCTTGTAACCATTACCCTTGACACTAACATGGTAAC‑TTTCGCTACCTTCA‑TGGCCGCTCCCCGCTCGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTGGATGACTGGCTGCTATCGGCTGTCGGGC‑TCACCGGTGTTTCCGGAGCTGGACCACCCGGGG‑TCAAAGCCCACCCATTATAATCCGGCtt                                                  >  2:410526/1‑251 (MQ=255)                                                                                                                                                                                                        gTTTTCAGCGTGGGTACCCCGGGGGCATTTTTTAAACCCAACCCACCGGTTGTTCTTTAAAACATCACCATTGAAAAAAACATGATAAT‑TTCCGCTACCTCCC‑GGGCCGCTCCCCGCTGGCAATGAGTAAGCTTTAACGCTACCCGCGGGTGTGTAGGGCGCGCTGTTATCCGGAGTGGGGG‑CCCCCGGTGTTTCCGGAGTTG‑AAGAAACGGTGCTCACAGCACCGCCATTATTATCCGGCTTAACGGaa                                           <  2:26105/250‑1 (MQ=255)                                                                                                                                                                                                        gTTTTCAGCGCTGGTACCCCGAGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAACATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGTG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAAc                                          >  1:431381/1‑251 (MQ=255)                                                                                                                                                                                                             cAGCGTTGGTCCCCCGTGGGCAATTTTTAGACCCACCCCATCAGGGTTTTTTGAAAACATTCCCATGAAAAAAAACATGATAAT‑TTTCGCTCCCTCCC‑TGGCCGCCCCACGCTTGCAAGGAGTAACCGTTAACGTTACCCGCGGGTGTTGATGGCTCGCTGTTATCGGGAGTTGGGG‑TCACCGGTGTTCCGGGAGTTGGATCAACGGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGa                                      <  1:379493/250‑1 (MQ=255)                                                                                                                                                                                                                    ggTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGt                              >  1:270509/1‑251 (MQ=255)                                                                                                                                                                                                                       aCCCCGTGGGCAATCTTTAGACCCAACCCCTCAGGCGTCCTTGTACACATTACCATTGAAAATAACCTGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAAAGCTATCCGCTGGTGTTGATCGCTCGATGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCCCCGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAc                           >  1:403981/1‑251 (MQ=255)                                                                                                                                                                                                                        ccccgggtgcaatgttaacacccaacccaTCGGGCGTTTTTGAAAACACTCACATAAAAAATAAC‑TGGAAAAATTTGGCT‑CCTTCCCTGGCCGCTCCCCGCTTGCAGAGAGTAATCGTAAACGCTCTCCGGGGGTGTGGAGGGCCCGCTGTTATC‑GGGGTTGGGGGCCCCCGGTGTTCCGGGGGTGGGACCACCCGGGG‑CCCCAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAcc                          <  2:89273/232‑1 (MQ=255)                                                                                                                                                                                                                             tGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACt                     >  1:316019/1‑251 (MQ=255)                                                                                                                                                                                                                                 cAATCTTTAGACCCAAACCATCAGGAGTTCTTGTAAACATTACCATTGAAACTAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCt                 >  2:35940/1‑251 (MQ=255)                                                                                                                                                                                                                                   aTCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTgg               >  2:52065/1‑250 (MQ=255)                                                                                                                                                                                                                                   aTCTTTAGACCCAACCCATCAGGCGATCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTg                >  1:347956/1‑250 (MQ=255)                                                                                                                                                                                                                                               aacccaTCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccg   >  1:5485/1‑251 (MQ=255)                                                                                                                                                                                                                                                acccaTCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccgt  >  2:308697/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                          CCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAA‑TTTCGCTACCTTCC‑TGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGG‑TCACCGGTGTTTCCGGAGTTGGATCAACCGGAG‑TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGT  >  NC_000913/3468524‑3469013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑