Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,436,697	G→A	E60E (GAG→GAA)	trkA →	NAD‑binding component of Trk potassium transporters

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,436,697	0	G	A	98.4%	187.4 / ‑5.9	64	E60E (GAG→GAA)	trkA	NAD‑binding component of Trk potassium transporters
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (31/31);  minor base C (1/0);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

GAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGC  >  NC_000913/3436455‑3436940                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    gagGGCGACGGCTTCTTTTACGCTAAGCTAATCAAACAGTGATGAGATAACGGCTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGc                                                                                                                                                                                                                                             >  1:23194/1‑251 (MQ=255) gagGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGc                                                                                                                                                                                                                                             >  1:317956/1‑251 (MQ=255)  agGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAggggtt                                                                                                                                                                                                                                            >  1:96219/1‑247 (MQ=255)  agGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGcc                                                                                                                                                                                                                                            <  1:245706/251‑1 (MQ=255)          ggCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGc                                                                                                                                                                                                                                    >  1:347745/1‑251 (MQ=255)               cttTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGatc                                                                                                                                                                                                                               >  1:297376/1‑250 (MQ=255)                 tttACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGatag                                                                                                                                                                                                                              <  2:91797/250‑2 (MQ=255)                 tttACGCTAAGCTAAGCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGTTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCATGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGata                                                                                                                                                                                                                               <  2:271962/249‑1 (MQ=255)                      gctaagctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGt                                                                                                                                                                                                                        <  1:94716/251‑1 (MQ=255)                         aagctaaTCAAAAAGTGATGAGATAACGGTTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACAGTGGCGGAAAACCGGGTTGGCTAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTTACCTGCGGGTCGTTCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGTCTACGACGCCTATATGCTGGtt                                                                                                                                                                                                                       <  2:277637/249‑1 (MQ=255)                         aagctaaTCAAAAAGTGATGAGAGAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTTTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAAGTTGGCCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGc                                                                                                                                                                                                                     <  2:297376/251‑1 (MQ=255)                              aaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTaa                                                                                                                                                                                                                <  1:26487/251‑1 (MQ=255)                                  aaaaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGa                                                                                                                                                                                                                                 <  1:85309/230‑1 (MQ=255)                                  aaaaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGa                                                                                                                                                                                                                                 >  2:85309/1‑230 (MQ=255)                                     aaGTGATGAGATACCGGGTCGAGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTc                                                                                                                                                                                                         >  1:323242/1‑251 (MQ=255)                                         gatgaGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGCACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCCGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCCGGTGCAGACGACGCCCATATGCTGGGTGccgcaacccgtttagct                                                                                                                                                                                                     >  2:316060/1‑235 (MQ=255)                                           tgaGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATCTGCTGGTTGCTGTCACCAGTTCAggtg                                                                                                                                                                                                    >  2:132665/1‑247 (MQ=255)                                           tgaGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATg                                                                                                                                                                                                    >  1:149222/1‑250 (MQ=255)                                             agaTAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAAACTGGATGGCGAGAACAACGATCTTACTGTTGGCGATAACAACGGAGAGCGTCTGCGGTACTTACAGGATACATTTGACCTGCGGGTCGGTCAAGGGCATGGCTCCCATCCACGCTTATTGCGGGAAGCAGGGCCCGAAGACTCCGATATGCAGGTTGCTGTTACCTGTTCAGATGaa                                                                                                                                                                                                  >  2:322268/1‑250 (MQ=255)                                                    gggTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGCTCTGCTGGTTGCTGTAACCAGTTCAGATGAAACCAatat                                                                                                                                                                                          >  2:299189/1‑251 (MQ=255)                                                    gggTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCAGGGCTCTCATCCGCGCGTATTGCGGGAAGCAGGGGCGGACGACTCGGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAatat                                                                                                                                                                                          <  1:377835/251‑1 (MQ=255)                                                     ggTCGAGACTGATGAAAATTATAATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCCGACAACCTGGTTGGCGAGAACACCGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATCAATTTGACCTGCGGGTCGTGCAGGGGCCTGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGCCGACGCCGATATGCTGGTTGCTGTCACCAGTCCAGAGGAACCCAAAATg                                                                                                                                                                                         >  2:405336/1‑251 (MQ=255)                                                        cgcgACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAGCCACGCGGATTGCGGGAAGCAGGTGCCGACGACGCCGGTATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGt                                                                                                                                                                                       <  1:409845/250‑1 (MQ=255)                                                                    aaaTTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACCACGATATTACTGTTGTCGATACCAACGGGGAGCGTCTGCGGACCTTACAGGCTAGATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGt                                                                                                                                                                           >  1:224500/1‑250 (MQ=255)                                                                            aTTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTc                                                                                                                                                                  <  1:200196/251‑1 (MQ=255)                                                                                 gggTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGATGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACt                                                                                                                                                               <  1:166271/249‑1 (MQ=255)                                                                                        ggCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGGAGCCTACTCACTTTTCAacac                                                                                                                                                      >  1:229229/1‑251 (MQ=255)                                                                                          ccAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACAccc                                                                                                                                                    <  1:14428/251‑1 (MQ=255)                                                                                          ccAGGTTGGAGGCACACTGGCGGAAAACCTGGTTGGCGAGCGCAAAGATATTACTGTTGTTGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCCGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACAccc                                                                                                                                                    >  1:261744/1‑251 (MQ=255)                                                                                                   cggcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGCTCAGATGAAACCAATATg                                                                                                                                                                                         <  2:308023/205‑1 (MQ=255)                                                                                                   cggcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGCTCAGATGAAACCAATATg                                                                                                                                                                                         >  1:308023/1‑205 (MQ=255)                                                                                                    gtcacaCTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAatcgcat                                                                                                                                          <  1:129527/249‑1 (MQ=255)                                                                                                               ggAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACGCACTTTTCAACACCCCTAATCGCAGCGCTCGTATcc                                                                                                                               <  2:215346/251‑1 (MQ=255)                                                                                                                     ccTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCAc                                                                                                                         <  1:299383/251‑1 (MQ=255)                                                                                                                      cTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACACACGGCGAGCGTCTGCGGACCTTAAAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGAAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCAcc                                                                                                                        >  1:49341/1‑251 (MQ=255)                                                                                                                          ttGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCCGAc                                                                                                                    >  2:337560/1‑251 (MQ=255)                                                                                                                           tGGCGAGAACAACGATATTACTGTTGTCGATACCAAGGGAAAGCGTCAGAGGCCCTTACAAGATCAATTAGAACTAAGGGTAGTGCAGGAGAAAGGCTCTCATCAACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTCACTAGTTCAGATGAACCCAATATGGTACCCTGCCAGGTAGCCTACTCACTTTTCACCACCCCTAATCGCATCGCTCGTATCCGATCActcgact                                                                                                                   >  1:260484/1‑245 (MQ=255)                                                                                                                           tGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACt                                                                                                                   >  1:379931/1‑251 (MQ=255)                                                                                                                               gagaACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGt                                                                                                               >  2:182271/1‑251 (MQ=255)                                                                                                                                agaACAACTATATTACTGGTGTCTATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAAGTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAGTGCTGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTACAACACCCCTAATCGCATCGCGCGTATCCGCTCACCAGACTACGTg                                                                                                              <  2:252292/251‑1 (MQ=255)                                                                                                                                       cGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGCATAAATTTGACCTGCGGGTCGTGAAGGGGCATGGTACTCATCCACGCGTATTGCGGGACGCAGGTGACGACGCCGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCCCTTTTCAACACCCCTACTCGCATCGCTCGTATCCGCTCACCAGACTACGTGcgcgctg                                                                                                       >  1:397920/1‑248 (MQ=255)                                                                                                                                                 gttgtCGATACCATCGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCGGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCGACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCt                                                                                             <  1:190352/251‑1 (MQ=255)                                                                                                                                                    gtCGATACCAACGGTGAGCGGCTTCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTGGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACGCACGTTTCAACGCCCCTAATCGCATCGCTCGGATCCGCTCACCATACTACGTGCGCGATGCCGATAAGCTAt                                                                                           <  2:49341/250‑1 (MQ=255)                                                                                                                                                      cGATACCAACGGTGAGCGTCTGCGGCCCTTACAGGATAAATTTGACCGGCGGGTCGGGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGGTCAGATGAAACCAATATGGTTGCCTGCCAGGTATCCTACTCACTTTTCAACTCCCCTAAGCGCATCGCTCGTATCCGCTCACCAGTCTACGTGCGCGATGCCGATAAGCTAttt                                                                                         <  2:406580/250‑1 (MQ=255)                                                                                                                                                        aTACCAACGGTGAGCGTCTGCGGACCTTACAGGATACATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGCGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCATACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGAATACGTGAGCGATGCCGATACGCTATttcat                                                                                      >  2:359843/1‑251 (MQ=255)                                                                                                                                                        aTACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATttcat                                                                                      >  2:147132/1‑251 (MQ=255)                                                                                                                                                        aTACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCGGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACTTGCGCGATGCCGATAAGCTATttcat                                                                                      <  1:343778/251‑1 (MQ=255)                                                                                                                                                         tACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCCTCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATttcatt                                                                                     >  2:200806/1‑251 (MQ=255)                                                                                                                                                                      cgccTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGGTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCCCACCAGTCTACGTGCGCGATGCCGATAATCTTTTTCATTCAGATGCTGTGcc                                                                        <  2:224500/248‑1 (MQ=255)                                                                                                                                                                          tGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGGAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGCCGCCGATATGCTGGTTGCTGTAAACAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGAtt                                                                    >  1:286573/1‑251 (MQ=255)                                                                                                                                                                           gCGGACCTTACAGGATAAATTTGACCTGCGGGGCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGAt                                                                     <  2:78781/249‑1 (MQ=255)                                                                                                                                                                             ggACCTTACAGGATAAATTTGCCCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGat                                                                 >  2:143481/1‑251 (MQ=255)                                                                                                                                                                             ggACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGat                                                                 >  1:316485/1‑251 (MQ=255)                                                                                                                                                                                           aaaTTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCAc                                                    <  1:308182/250‑1 (MQ=255)                                                                                                                                                                                             aTTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCag                                                 <  2:347745/251‑1 (MQ=255)                                                                                                                                                                                             aTTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCag                                                 <  1:132665/251‑1 (MQ=255)                                                                                                                                                                                              tttGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACCCAGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGCAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCAcc                                                   >  1:388058/1‑248 (MQ=255)                                                                                                                                                                                                            cGTGCGGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCg                                  <  2:323242/251‑1 (MQ=255)                                                                                                                                                                                                                 aGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGTAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACCCCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGTGCGATGCCGATAAGCTATTTCATTCTGATTCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGataat                             <  2:205612/251‑1 (MQ=255)                                                                                                                                                                                                                  ggggCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCCTTCAGATGCTGTGCCGATTGCTCATCTGATCGCACCAGAGCAGTTGGTTATCGataat                             >  2:268622/1‑250 (MQ=255)                                                                                                                                                                                                                    ggCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCTTAGCCGCTCACCAGTCTACATGCGCGATGCCGATAATCAATTGCATTCAGAGGCTGTGCCGATTGATCTTCTGATCGCACCAGAGCAGTTGTTTATCGATAATAtt                          <  2:383403/251‑1 (MQ=255)                                                                                                                                                                                                                                      gcgTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTAt        <  1:33633/251‑1 (MQ=255)                                                                                                                                                                                                                                          aTTGAGGGATGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGGTCATATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATccc     <  2:20151/250‑1 (MQ=255)                                                                                                                                                                                                                                            tGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGgc  >  1:343057/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    GAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGC  >  NC_000913/3436455‑3436940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑