Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,321,967	A→G	intergenic (‑354/+105)	yhbX ← / ← leuU	putative hydrolase YhbX/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,321,967	0	A	G	100.0%	146.2 / NA	44	intergenic (‑354/+105)	yhbX/leuU	putative hydrolase YhbX/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/25);  total (19/25)

TTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGAGC  >  NC_000913/3321720‑3322208                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  ttttttCTTTTTAAATGATGCGGCCATTGCGTTCTCAGCAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGAATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCt                                                                                                                                                                                                                                                >  2:368371/1‑251 (MQ=255)       cTTTTTAAATGATGAGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGGAGAACGTAGCCATCAGTCGTTATCCTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTAAAGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGAACCGGCTACGaa                                                                                                                                                                                                                                           >  2:129014/1‑249 (MQ=255)              aaTGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCaa                                                                                                                                                                                                                                   <  1:51232/251‑1 (MQ=255)                      ggCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGGAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTAGCGAGAAGTAAACATATCCTTATTCGGACCGGAACTGGAGGTGACACAGTAAAGTGCCAAGTATGTCAGTAGGATGGAAAAATGGATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACga                                                                                                                                                                                                                           <  1:23226/251‑1 (MQ=255)                        ccATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTCAACATTAAGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACg                                                                                                                                                                                                                            >  1:220879/1‑248 (MQ=255)                                          ttAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGCCGAGCTACAGATACCTGACAAcc                                                                                                                                                                                                       >  2:240704/1‑251 (MQ=255)                                              aTAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTc                                                                                                                                                                                                   <  1:315186/251‑1 (MQ=255)                                                aaCATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCt                                                                                                                                                                                                 <  1:337094/251‑1 (MQ=255)                                                  cATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTc                                                                                                                                                                                                >  1:53786/1‑250 (MQ=255)                                                      aTCAATGCGTCTCTAAACAGTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGAcc                                                                                                                                                                                           <  1:129014/251‑1 (MQ=255)                                                               tccatAAACATTGCGCAGAACGTAGCCATCAGTCCTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGATCTGGAGTTGACACAAGAAAGCGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAATGCGTACCGGCACCATCTGTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACTAGATACAGATACCTTACAACCATGCCTCagaccaagacca                                                                                                                                                                                    <  2:263528/245‑1 (MQ=255)                                                                  ctAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCaaaa                                                                                                                                                                                 <  2:338236/249‑1 (MQ=255)                                                                       cATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGaaaa                                                                                                                                                                           >  2:321508/1‑250 (MQ=255)                                                                             gCAGAACGTAGCCCTCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCCGACCAGGACCAAAACGAAAAAAGACGc                                                                                                                                                                    >  2:64116/1‑251 (MQ=255)                                                                             gCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCGGACCAGGACCAAAACGAAAAAAGACGc                                                                                                                                                                    >  1:414421/1‑251 (MQ=255)                                                                               aGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCtt                                                                                                                                                                  >  2:58642/1‑251 (MQ=255)                                                                                 aaCGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTGCAAGGTACCGGCTACGAGTAAAGCAACGGGACGAGATACAGATTCCTGACATCGATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCtttt                                                                                                                                                                <  2:212930/251‑1 (MQ=255)                                                                                             aGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCttt                                                                                                                                                    <  1:64116/251‑1 (MQ=255)                                                                                                cGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGATACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTGTTCt                                                                                                                                                 <  2:15359/251‑1 (MQ=255)                                                                                                 gTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTg                                                                                                                                                <  1:368371/251‑1 (MQ=255)                                                                                                      aCTGACAGGAGCAGGATGGTTTAACGAGAAGTAAACATATCCGTATTCGTACCGGAACTGTAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGTCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTGCTTGAAtt                                                                                                                                           <  1:299179/251‑1 (MQ=255)                                                                                                                    gATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAAGAAAGGGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATGTGGTACCGAGGACg                                                                                                                             <  2:156821/251‑1 (MQ=255)                                                                                                                           tATCGAGAAGAAAAAATAGCCATATTCGTACCGGAAATGGCGTTGCCACAATAAAGTGCCAATTATGGCAGTAGAAGGGAAAAATTTATAAATAAGGCGTACCGGCACCATCGTTTCAAGGAACCGGCTACGAGTAAAGCAACTGGACGAGCTACAGCTACCAGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAAACGCTCTTCAGCGTCTCTCTTCGGGAATTTGGTACACAGGAAGGGACTTg                                                                                                                      >  2:155830/1‑251 (MQ=255)                                                                                                                              cGAGAAGTAAACATATCCGTATTCGTACCGGGACTTGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGTGAAAAATTTATAACTAAGGCGTCCCGGCACCATCGTTTCAAGGTTACGGCTACGAGTAAAGCAACTGGACTAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACTAAAAAAGACGCTTTGCAGCGTCTCTTTTCTGGAATTTGGTACCGATGACGGGACTTGAAc                                                                                                                   <  2:5772/251‑1 (MQ=255)                                                                                                                                agaAGTAAACATATCCGTATTCGTACCGGACCTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGATACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGCAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATATGGTACCGAGGACGGGACTTGAAccc                                                                                                                 >  2:74448/1‑251 (MQ=255)                                                                                                                                        aCATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGccc                                                                                                         >  2:105323/1‑251 (MQ=255)                                                                                                                                                gTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                                                                 <  1:231599/251‑1 (MQ=255)                                                                                                                                                   ttcgttgcGGAACTTGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCTCCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACTCTTTTCAGCGTCTCTTTGTTGGAATTTTGTACCGAGGACGGGACTTGAACCCGGAAGCGCTAtgggtcac                                                                                               <  2:274370/243‑8 (MQ=255)                                                                                                                                                                    gTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGCTACCTGACAACCATTCCTCAGACCAGGCCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGTGACTTGCACCCGTAAGCCCTATTGGGTACTACCACCTCAATGTAGCg                                                                             >  2:313304/1‑251 (MQ=255)                                                                                                                                                                     ttGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATAGTTTCACGGTACCGGCTACGAGTAAAGCCACTGGCGGAGATACAGTTACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGCGGCTTTTCAGCGGCTCTTTTCTGGAATTTGGTACCGAGGACGGGGCTTGAACCCGTAATCCCTATTTGGCCCTACCACCTCACGGTcgcgg                                                                            >  2:316314/1‑250 (MQ=255)                                                                                                                                                                        acacAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                                                                         <  1:47288/251‑1 (MQ=255)                                                                                                                                                                               aaaGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCATACCAGGACCAAAACGAAAAAAGACTCTTTTCAGCGTCTCTTTTCTGGAATTTTGTACCGAGGACGGGACGTTAACCCTTAAGCCCTATTGGGCACTACCGCCTCAAGTTTGCGCGTCTACCAAt                                                                  <  2:266964/251‑1 (MQ=255)                                                                                                                                                                                      cAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccacc                                                            >  1:378430/1‑250 (MQ=255)                                                                                                                                                                                          tATGTCCGTAGCAGGGACAAATTTATAACTAAGACCTACCGGAACCATCGTTTCAAGGTACCGGCTACGAGCAAAGCAACTGCCCGCGATACAGCTACCTGACAACCATTCCTCAGACCAGGCCCACAACGAAAACAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTc                                                       >  2:288737/1‑251 (MQ=255)                                                                                                                                                                                           aTGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTc                                                       <  1:222020/250‑1 (MQ=255)                                                                                                                                                                                                 gTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACgg                                                <  1:315573/251‑1 (MQ=255)                                                                                                                                                                                                        ggAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCGCGGCACGGAtactac                                         <  2:293294/251‑1 (MQ=255)                                                                                                                                                                                                            aaaTTGATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCGTTTCAGCGTCTCTTTTCTGGAAGTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGTCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGGACGTATACGACtttt                                     <  2:169613/251‑1 (MQ=255)                                                                                                                                                                                                             aaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACttttt                                    <  1:321508/251‑1 (MQ=255)                                                                                                                                                                                                                   taACTAAGGCGTACCGGCACAATCGTTTCAAGGTACAGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCg                              >  1:139696/1‑251 (MQ=255)                                                                                                                                                                                                                         aGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGAc                                                                                                                              <  2:190372/149‑1 (MQ=255)                                                                                                                                                                                                                         aGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGAc                                                                                                                              >  1:190372/1‑149 (MQ=255)                                                                                                                                                                                                                               aCCGGCACCATCGTATCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTgg                  >  2:172105/1‑251 (MQ=255)                                                                                                                                                                                                                                               cAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCGGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGTTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGagc  <  2:414421/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  TTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGAGC  >  NC_000913/3321720‑3322208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑