Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,216,473	A→G	Y7H (TAC→CAC)	nfeF ←	NADPH‑dependent ferric‑chelate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,216,473	0	A	G	97.7%	121.1 / ‑4.6	44	coding (19/765 nt)	nfeF	NADPH‑dependent ferric‑chelate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (10/33);  minor base . (0/1);  total (10/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCTATAGT‑CTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTG  >  NC_000913/3216228‑3216719                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           ataGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCggg                                                                                                                                                                                                                                                       <  1:88943/251‑1 (MQ=255)    gTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGACGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCgggggg                                                                                                                                                                                                                                                    <  1:6541/251‑1 (MQ=255)       aCGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGGCAGTGCACGGAAGCGCAGATCATTGCGAACGCGCGGCGGGTGGCGGGGGgtg‑‑t                                                                                                                                                                                                                                               <  2:303508/251‑1 (MQ=255)           gactgtgggcgtgggcCTTCCGGCCAGACGATGCCCGCGTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCATGTTGAGGAAAGAAGAGTTTGCTTGGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCGCGCCGCCGAGGTCAATGCGCTGAAAACCGGCGTTGATGCGCTCAAC‑GCGTAGCACAGTGAGTTCACGGGAGCGCATATCATTGCGAACGCGCTGGGGGTGGCGGGGTGGG‑‑TTAtt                                                                                                                                                                                                                                           <  2:370156/247‑1 (MQ=255)               gTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGTCGGTGGGTG‑‑TTATTCAtt                                                                                                                                                                                                                                       <  1:108519/251‑1 (MQ=255)                 gggCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTg                                                                                                                                                                                                                                      <  1:203450/250‑1 (MQ=255)                 gggCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGGG‑‑TTATTCATTGt                                                                                                                                                                                                                                     >  2:235379/1‑251 (MQ=255)                      gggTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAAGGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCGGCGGGTGGCGGGGGGTG‑‑TTATTCATGGTTATCg                                                                                                                                                                                                                                <  2:174488/250‑1 (MQ=255)                       ggTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCg                                                                                                                                                                                                                                <  1:164309/250‑1 (MQ=255)                        gTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGcc                                                                                                                                                                                                                              <  1:148232/251‑1 (MQ=255)                          ccTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCt                                                                                                                                                                                                                             <  1:219335/250‑1 (MQ=255)                                  ccAGACGATGCCCTCTTCCGTTACCGGTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATgg                                                                                                                                                                                                                    <  2:53136/251‑1 (MQ=255)                                         aTGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATc                                                                                                                                                                                                               >  1:307910/1‑249 (MQ=255)                                            cccTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGata                                                                                                                                                                                                          <  1:209999/251‑1 (MQ=255)                                                  tCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATc                                                                                                                                                                                                      <  1:104126/249‑1 (MQ=255)                                                              ggcggcACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCATCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGGG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGc                                                                                                                                                                                        >  2:187716/1‑251 (MQ=255)                                                                     caAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAaatgat                                                                                                                                                                                 <  1:11313/251‑1 (MQ=255)                                                                             gCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTGATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACCCGCAAATGATAATGATTg                                                                                                                                                                         <  2:54425/251‑1 (MQ=255)                                                                              cGTCAGGTTGAGGAAAGAAGAGTGTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGt                                                                                                                                                                        <  1:418503/251‑1 (MQ=255)                                                                               gTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGtt                                                                                                                                                                       >  1:156352/1‑251 (MQ=255)                                                                                   ggTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCCTTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATc                                                                                                                                                                   >  1:419948/1‑251 (MQ=255)                                                                                          gAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATaa                                                                                                                                                            <  1:70605/251‑1 (MQ=255)                                                                                                 gagTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCAGGATAAATGCAAg                                                                                                                                                     <  1:142135/251‑1 (MQ=255)                                                                                                  ggTTTTCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAAGGATAATGATTGTTAATCATGATAAATGCAAGc                                                                                                                                                    <  2:4913/250‑1 (MQ=255)                                                                                                          gtgATCGTCAAAGCCACGCGATGTAAAACCATCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTg                                                                                                                                              <  1:187716/249‑1 (MQ=255)                                                                                                            gATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATAGCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAg                                                                                                                                           <  1:225940/250‑1 (MQ=255)                                                                                                             aTCGTCAAAGCCATGCGATGTAAAACCGTCCAGCGCCTCACCGCCCAGGACAATGCGCTGAAAACTGGCGCTTATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCCGCGGGTGGAGGGGGGCG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAACCGCGCAAATGATAATGATTGTTAATCATTATAAATGCAATCGATTTGTAGa                                                                                                                                          >  1:18592/1‑250 (MQ=255)                                                                                                               cGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAAc                                                                                                                                        <  1:9061/250‑1 (MQ=255)                                                                                                                gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGGCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCAAAATAAAACACGCAAATGTTAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑g                                                                                                                                     <  1:396815/251‑1 (MQ=255)                                                                                                                                                              aCAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTCACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCTATAGT‑CTGATAAGACGAACCGCCTCTTCTCAGGcatc                                                                                       >  2:63366/1‑250 (MQ=255)                                                                                                                                                                aaTGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCtat                                                                                                                           <  2:301487/213‑1 (MQ=255)                                                                                                                                                                aaTGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCtat                                                                                                                           >  1:301487/1‑213 (MQ=255)                                                                                                                                                                              cGGCGCTGATGCGC‑CAACCGCGTAACACAGTAAGTTCACGGAAGCGCAGATCATTGCGAACGCGTGGCGGGTGGGGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCTATAGT‑CTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGAt                                                                      <  2:307910/251‑1 (MQ=255)                                                                                                                                                                                  gcTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCTATAGT‑CTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATgcgg                                                                  >  1:388012/1‑251 (MQ=255)                                                                                                                                                                                     gaggcgcTCAAC‑GCGTAACCAAGTCAGTACCCGGAAGCGCGGATCATGGCGAAGGCAGTGCGGGGGGCGGGGGTTG‑‑TTGTTCATTGTTATCGCCTTCGTGATGGTAATCAAATATATCAAAAAAAAACTCGCAAAGGAAAATTATTTTTATTCATGATAAAGCCAGGCGAGTGATAGATCG‑AATATGTCTATGGT‑ATAATAAGACAAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATgcggcg                                                                <  2:419948/247‑1 (MQ=255)                                                                                                                                                                                         cgcccAAC‑GCGTAACACGGGCAGTCCACGGAAGCGAAGACCATGGCGAGCGCGCTGAGGGGGGGGGGGGGTG‑‑TTATTCATTTTTCGCGCTTTCGTGATGGTATTCAGATATATCTAAATAAACCTCGAAAATGATAAGTATTTTTAATAATGTTAAAGGCAAGCAATTGGTAGAATG‑GATATGT‑TATTGTTCTGATAAAGCGAACCGCCGCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAAc                                                           <  1:184779/247‑1 (MQ=255)                                                                                                                                                                                                 cccgaaacagaggcagtTCACGAAAGCGCAGGTCATTGCGACCGCGGTGGGGGTGGCGGGGGGTT‑‑TTATTCATTGTAATCGCCTTCGTGATGGTAATAAGAAATATCTAAAAAAAACGCGCAAAGGATAGTGATTGTAAAACATGATAAATGCAAGCGATTTGTAGAACT‑GAGATTTTTATAGT‑CTGATAAGAGGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCTGATGCGGCGTGAACGCCTTAt                                                    <  2:388012/238‑1 (MQ=255)                                                                                                                                                                                                                   cACGGAAGCGAAGATTGTGGCGAGCGCGCGGCGGGTGGGGGGGGGTG‑‑TTTTTCATTTTTATCGCTTTCGTGATGGTAATCAGATATATTTAAATAAAACACGAAAAGGATAATGATTTTTAATCTTGATAAATGCAAGCGATTTGTAGAACT‑ATTATGTCTATATT‑CTGATAAGCGGAGCCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGAt                                  <  1:281856/251‑1 (MQ=255)                                                                                                                                                                                                                        aaGCGCATATCATTGCGAGCGCTTTGCGGGTGGGGGGGGGTG‑‑TTATTCATTGTTCTCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAGTGATAATGATTGTTAATCATGAAAAATGCAAGCGATTTGTA‑AACTTGATTTGTAGATGGT‑CTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTc                             <  1:30783/251‑1 (MQ=255)                                                                                                                                                                                                                        aaGCGCAGATCATTGCGAACGCGCTGCGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCTATAGT‑CTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTc                             >  2:219888/1‑251 (MQ=255)                                                                                                                                                                                                                        aaGCGCAGATAATGGCGAACGCGCGGCGGGGGGGGGGGGGTG‑‑TTATTCATTGTTATCGCCTGCGTGATGGTAATCAGATATATCTAAATAAAACTCGAAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCTATAGT‑CTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTc                             <  1:63366/251‑1 (MQ=255)                                                                                                                                                                                                                           gccggATCATTGCAAACGCGGTGGGGG‑‑GGGGGGGGTGGTTTATTAATTGTTATCTCCTGTGTGATGGTTATCATATATATAAAAAAAAATCTCGAAAATGATAATGATTGTTAATCATGAAAAATGCAAGCGTTTTGTAGAACG‑GATATGTCTATAGT‑GTGTTAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCAc                          <  2:361161/247‑1 (MQ=255)                                                                                                                                                                                                                             cAGATCATTGCGACCGCGCTGGGGGTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCTATAGT‑CTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGt                        <  1:235379/251‑1 (MQ=255)                                                                                                                                                                                                                                                   gggTGGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCTATAGT‑CTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTg  <  1:70833/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           ATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAC‑GCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGTG‑‑TTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACT‑GATATGTCTATAGT‑CTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTG  >  NC_000913/3216228‑3216719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑