Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	263,050	T→C	intergenic (+104/+278)	thrW → / ← ykfI	tRNA‑Thr/toxin of the YkfI‑YafW toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	263,050	0	T	C	100.0%	39.8 / NA	12	intergenic (+104/+278)	thrW/ykfI	tRNA‑Thr/toxin of the YkfI‑YafW toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/7);  total (5/7)

CGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCT  >  NC_000913/262815‑263096                                                                                                                                                                                                                                            |                                               cGGTTAGTTTTAACCTTGTCCACCGTGCTTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTACCTACTGACCaaaa                                   >  2:262015/1‑249 (MQ=255)   gTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGACGATCAATCTTACCTACTGACCAAAAAGGc                               >  2:99005/1‑251 (MQ=255)     tAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTCTCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTACCTACTGACCAAAAAGGCCt                             >  2:353852/1‑251 (MQ=255)         tttAACCTTGTCCTCCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTGATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAAGTCTATCAACGAAGATCAATCTTACCTACTGACCAAAAAGGCCTGAt                          <  2:187996/250‑1 (MQ=255)          ttAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTACCTACTGACCAAAAAGGCCTGATa                         <  2:77719/250‑1 (MQ=255)            aaCCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGGTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTGCGTAAGAGCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTACCTACTGACCAAAAAGGCCTGATAgga                      <  1:396782/251‑2 (MQ=255)                  gTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTACCTACTGACCAAAAAGGCCTGATAggataaatc                <  1:353852/251‑8 (MQ=255)                      aCCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTACCTACTGACCAAAAAGGCCTGATAggataaatctcg             >  2:86067/1‑240 (MQ=255)                           gATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCATAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTACCTACTGACCAAAAAGGCCTGATAggataaatctcgaaaat        <  2:95837/250‑16 (MQ=255)                            aTTCACGTTCGTGAACAGTTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAATGTCTGAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCAGTCTCCCACCAAAAAATTATCTTAATTTAGCAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAACCTTACCTACTGACCAAAAAGGCCTGATAggatatatctcgataataa      <  2:251902/251‑18 (MQ=255)                               cACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAAATATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTACCTACTGACCAAAAAGGCCTGATAggataaatctcgaaaataat     >  1:144250/1‑231 (MQ=39)                                aCGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGGTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGTCTCCTATTATCGACACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGATGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTACCTACTTACCAAAAAGGTCTGGTAggataaatctcgaaaataataga  <  1:355696/251‑22 (MQ=255)                                                                                                                                                                                                                                            |                                               CGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCT  >  NC_000913/262815‑263096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑