Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,925,117	G→A	P55L (CCG→CTG)	syd ←	SecY‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,925,117	0	G	A	100.0%	165.8 / NA	46	P55L (CCG→CTG)	syd	SecY‑interacting protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/18);  total (28/18)

AGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTGAAACATGTCTTCTTATGCA  >  NC_000913/2924871‑2925362                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      aGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTg                                                                                                                                                                                                                                                   >  1:157174/1‑251 (MQ=255)                         ggcAGTGTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTTCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGTGTTTCCAGTATACGGCATCTTc                                                                                                                                                                                                                           <  2:395221/251‑1 (MQ=255)                                              gTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACgg                                                                                                                                                                                                      >  1:241838/1‑251 (MQ=255)                                                     gATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAgg                                                                                                                                                                                                >  1:25354/1‑250 (MQ=255)                                                      aTGACCAATCAGATTTGCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATAGCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGGATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGGAAACAGCTGGGGTGGCCAGGATACTGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGaa                                                                                                                                                                                              <  2:406056/251‑1 (MQ=255)                                                            aaTCAGATTTTCCGGCCCCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTGGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTGCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGt                                                                                                                                                                                        <  2:318685/251‑1 (MQ=255)                                                                                gACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGc                                                                                                                                                                    >  1:121409/1‑251 (MQ=255)                                                                                  cGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGcc                                                                                                                                                                   >  2:333563/1‑250 (MQ=255)                                                                                   ggAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAg                                                                                                                                                                 <  2:154933/251‑1 (MQ=255)                                                                                    gAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAACCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGc                                                                                                                                                                >  2:345204/1‑251 (MQ=255)                                                                                                  cccAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACGGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACTCCGGACAGTTCCTCACTTAACGGCCAGCTTTGATGCTCTTCa                                                                                                                                                  <  2:157174/250‑1 (MQ=255)                                                                                                     aGGTTTGCAGCAATGTCAGTTGGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAATCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGTTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACTCCGTACAGTTCCCCACTTAACGGCGAGCTTTTATTCACTTCATGc                                                                                                                                               <  2:248906/251‑1 (MQ=255)                                                                                                             agcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCGCTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATc                                                                                                                                       <  1:156278/251‑1 (MQ=255)                                                                                                              gcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCg                                                                                                                                      <  1:334812/251‑1 (MQ=255)                                                                                                                aaTGTCAGTTTGATATCGCCAACATGCGCGTTCATAGCCCCGGCAAACTGATTCGTATAAAAGGGATGTATTGTGGGGTGTATCACAATATCAAAAGCTCGTTCAACCGCGTTTACATTGTATTCCCCGGTAAACAGCAGGGGGTGCCAGTATACGGTATCTTCGGTAGTTGAAATAATGCGCGGTGAAGGAACCCCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCTTCGCa                                                                                                                                    <  2:418973/251‑1 (MQ=255)                                                                                                                aaTGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGCATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGc                                                                                                                                     >  1:219120/1‑250 (MQ=255)                                                                                                                   gTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAACGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGt                                                                                                                                  >  1:214999/1‑250 (MQ=255)                                                                                                                     cAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAAGGCACGGTTTAGGAACACCGTACAGTTCCTCTCTTAACGGCCAGCTTTTATGCGCTTCATTCCACGCATCACAGTAg                                                                                                                                <  2:277211/250‑1 (MQ=255)                                                                                                                       gTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGAATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGAAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGt                                                                                                                             >  1:217603/1‑251 (MQ=255)                                                                                                                         ttGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTg                                                                                                                            <  2:221176/250‑1 (MQ=255)                                                                                                                           gATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGACCACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGc                                                                                                                           >  2:315344/1‑249 (MQ=255)                                                                                                                                    aaaCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGAATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCtt                                                                                                                >  1:51604/1‑251 (MQ=255)                                                                                                                                                 atatCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCAAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGc                                                                                                   >  2:125747/1‑251 (MQ=255)                                                                                                                                                        cGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCaa                                                                                             >  1:423281/1‑250 (MQ=255)                                                                                                                                                                  aGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCTGTCAAATCGTCCACTa                                                                                  <  1:345204/251‑1 (MQ=255)                                                                                                                                                                             aGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCtt                                                                       <  1:369550/251‑1 (MQ=255)                                                                                                                                                                                tATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCAc                                                                    >  1:250828/1‑251 (MQ=255)                                                                                                                                                                                 aTGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACt                                                                   <  1:333563/251‑1 (MQ=255)                                                                                                                                                                                       tgtgGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCCTGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAAcc                                                             >  1:240578/1‑251 (MQ=255)                                                                                                                                                                                        gtgGGTTGTATCACAATATCAAAAGAGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAAcc                                                             >  1:349525/1‑250 (MQ=255)                                                                                                                                                                                                tATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACt                                                    >  2:71526/1‑251 (MQ=255)                                                                                                                                                                                                 aTCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACtt                                                   <  1:231031/251‑1 (MQ=255)                                                                                                                                                                                                  tCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACATTTCCTCACTTAACGTCCAGCTGTTATGCTCTTCATGCCAc                                                                                                                                            <  2:347090/161‑1 (MQ=255)                                                                                                                                                                                                  tCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCAc                                                                                                                                            >  1:347090/1‑161 (MQ=255)                                                                                                                                                                                                        tatCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCgttt                                            >  2:113151/1‑251 (MQ=255)                                                                                                                                                                                                         atCAAAAGCGCGTTAAACCGCGTTTCCATTTTGTTCACCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCgttt                                            >  2:395013/1‑250 (MQ=255)                                                                                                                                                                                                            aaaaGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCATGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCgtttagtt                                        <  1:197731/251‑1 (MQ=255)                                                                                                                                                                                                            aaaaGCGCGCTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAACTAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCCCTCATTTCTCCCTTCCCTTAAACCCGATACACTTGTCtgttagtt                                        >  2:123121/1‑251 (MQ=255)                                                                                                                                                                                                                 cgcgTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGCTACACTTGTCGTTTAGTTTATCt                                   >  1:149926/1‑251 (MQ=255)                                                                                                                                                                                                                           cgcgTTTACGTTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTaa                                                                >  1:312719/1‑212 (MQ=255)                                                                                                                                                                                                                           cgcgTTTACATGTTGTTCCCCGGTAAACAGCTGGGGTTTCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAATGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTaa                                                                <  2:312719/212‑1 (MQ=255)                                                                                                                                                                                                                                   cATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCCTCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTGAAAc                 >  1:298200/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCAACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTGAAAc                 >  1:217421/1‑250 (MQ=255)                                                                                                                                                                                                                                           tCCCCGGTAAACAGCTGCGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCAAGGTGACGGCACCACGTACAGTTCCTCACTTAACGGCCAGCTTTTCTGCTCTACATGCCACGCAACGCCGTAGCGTGCCGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCCCTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGGTTATGCCGGTGAAACATGtcttc         >  2:111704/1‑251 (MQ=255)                                                                                                                                                                                                                                           tCCCCCGTAACCAGCTGGGGTTGCCAGTATCCGGCATCTTCGGTAGTTGAAATACTGCACGGTGCAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATACCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCATATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTGAAACATttcttc         >  2:107386/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aaaCAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTGAAACATGTCTTCTTATGCa  >  2:244005/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      AGCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTGAAACATGTCTTCTTATGCA  >  NC_000913/2924871‑2925362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑