Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,868,452	T→C	N101S (AAC→AGC)	nlpD ←	murein hydrolase activator NlpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,868,452	0	T	C	100.0%	132.2 / NA	40	N101S (AAC→AGC)	nlpD	murein hydrolase activator NlpD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/23);  total (17/23)

GTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCAT  >  NC_000913/2868205‑2868691                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 gTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGcttt                                                                                                                                                                                                                                               >  2:102286/1‑251 (MQ=255)            aaaTGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTGCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTGCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTAcc                                                                                                                                                                                                                                    <  2:309257/250‑1 (MQ=255)             aaTGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTAcc                                                                                                                                                                                                                                    <  1:152966/250‑1 (MQ=255)                    tttCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGgctgct                                                                                                                                                                                                                             <  2:148239/250‑1 (MQ=255)                                           ggAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGTGCATGAATATTGTTGCGCTGCGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCActggctgg                                                                                                                                                                                                      >  1:33924/1‑250 (MQ=255)                                                    aCCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGTGCCTGAAGATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCCCGGTATATGTACTGCCGCTATAACTGCCTGTCGGAATGTTCCCATACTGACGGGTATAGACGATGCGGCCGCTTTCCATCTGGACCGGCGTCTGAGCTACTGGCTGCACTGGCTGGATGTGCGgt                                                                                                                                                                                             <  2:169541/250‑1 (MQ=255)                                                     cccACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGgttg                                                                                                                                                                                           >  1:180721/1‑251 (MQ=255)                                                               aGGTCTTACCAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGcc                                                                                                                                                                                 <  1:295859/251‑1 (MQ=255)                                                                     gACCAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAAtt                                                                                                                                                                           >  2:430957/1‑251 (MQ=255)                                                                       ccAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCCGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGCGTCGCCTTTTTTCACGGTATAGGTACTGACGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTtg                                                                                                                                                                         >  1:270532/1‑251 (MQ=255)                                                                           cGTTCAGCGCGTAGGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGgc                                                                                                                                                                     <  2:320398/251‑1 (MQ=255)                                                                             ttgaGCGCGGATGGTGCCTGGATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTGGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGGCCGCTTTCCATCGGTTCCGGCTGCTGAGCTACGGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTGGTGgct                                                                                                                                                                    <  2:383806/247‑1 (MQ=255)                                                                                 gcgcGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCtgctgc                                                                                                                                                               <  2:238299/251‑1 (MQ=255)                                                                                            gCCTGAATATTGTTGCGCTGGGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATACCTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCGGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAAt                                                                                                                                                    <  2:353505/251‑1 (MQ=255)                                                                                              cTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAAttt                                                                                                                                                  >  1:298895/1‑251 (MQ=255)                                                                                                    atTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTTGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGtt                                                                                                                                            <  1:175606/251‑1 (MQ=255)                                                                                                    atTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAAGCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAAGGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGtt                                                                                                                                            <  2:180721/251‑1 (MQ=255)                                                                                                            gcTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGGTGTTGGGTAGCCTGAATTTGTGGCGCCTGCCCCGGCTGAATTTTCGGTTGCTgcgcc                                                                                                                                    >  2:293639/1‑250 (MQ=255)                                                                                                               gAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTg                                                                                                                                                                                                 >  2:92409/1‑187 (MQ=255)                                                                                                               gAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTg                                                                                                                                                                                                 <  1:92409/187‑1 (MQ=255)                                                                                                                  cAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑c                                                                                                                             <  2:350722/251‑1 (MQ=255)                                                                                                                          cGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTCGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGGTGCTGCGCTGTAGA‑CGTCGTccc                                                                                                                     >  1:29974/1‑251 (MQ=255)                                                                                                                            gAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTccccc                                                                                                                   >  1:218949/1‑250 (MQ=255)                                                                                                                                 cGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTGGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTc                                                                                                              <  2:270532/251‑1 (MQ=255)                                                                                                                                 cGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTGTTTTCACGGTATAGGTACTGCCGCTATAACTTCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTc                                                                                                              <  2:347841/251‑1 (MQ=255)                                                                                                                                               cAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAAt                                                                                                <  1:277118/251‑1 (MQ=255)                                                                                                                                                  gcggtaaaagaaaGTGGGTCGTCTTTTTTCACGTGAGGGGTACTGCCGCTATAGCTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCAGCTGTGCCGGCGTCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGGTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTATAGA‑CGTCGTCCCCATATTCAGCGGCGGCGTAATCaa                                                                                             <  2:394696/241‑1 (MQ=255)                                                                                                                                                         aGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGGTTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCGGCGGTGTAGAACG‑CGTCCCCATTTTCGGCGGTCGCGTAAttcacctaacc                                                                                      >  2:256120/1‑241 (MQ=255)                                                                                                                                                             aggtgtggtcccgtttttCACGGGTTAGTTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGACGCGTCCGCTTTCCATCTGTGCCGGCTGCTGAGCTACTGGCTGCACTGGCTGTATTTGCGGTTGTTGAGTAGCCTGAATTTGCGGCTGCTGCTCCGGCTGAATTTGCGGTTGCCGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAAt                                                                                  <  2:293174/238‑1 (MQ=255)                                                                                                                                                                 gtgtCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGt                                                                              >  1:59013/1‑251 (MQ=255)                                                                                                                                                                           ttttCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGATGAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAgg                                                                     <  1:282512/250‑1 (MQ=255)                                                                                                                                                                                     ataGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCAt                                                          >  1:235945/1‑251 (MQ=255)                                                                                                                                                                                           aCTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACgg                                                    >  1:189709/1‑251 (MQ=255)                                                                                                                                                                                                  cTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGGAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGAc                                             >  1:114770/1‑251 (MQ=255)                                                                                                                                                                                                    ataACTGCCTTTCGGAATGTTCCCATACTGAAGGTTATCGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTCCTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGATTTTGTGGCTGCTGCACCCGCTGAATTTTCGGTTGCTGGGCTGTAGA‑CGTCGTCCCCCTTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTTCAGGCGCATTGCCCTTAACTGCGCTGAccg                                           >  2:385144/1‑251 (MQ=255)                                                                                                                                                                                                                            atcatGACGGTTAGAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTTCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCATAATTAGTATTTGCAGGCGCATTGCCATGAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAg                   <  2:194341/247‑1 (MQ=255)                                                                                                                                                                                                                               cTGACGGTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCCCCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGACTTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGGTTTGAAGTGTCAGAAc                >  2:157889/1‑251 (MQ=255)                                                                                                                                                                                                                                  aCGGTTATAGCCGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGc             >  1:330333/1‑251 (MQ=255)                                                                                                                                                                                                                                     gTTATAGACGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTTGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTg          <  1:126519/251‑1 (MQ=255)                                                                                                                                                                                                                                           gACGATGCGTCCGCTTTCCATCTGGACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATGTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTgccagcc    <  2:235541/251‑1 (MQ=255)                                                                                                                                                                                                                                             cGATGCGTCCGCTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCAt  <  1:19223/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGA‑CGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCAT  >  NC_000913/2868205‑2868691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑