Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,390,295	T→G	Q404P (CAG→CCG)	nuoN ←	NADH:quinone oxidoreductase subunit N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,390,295	0	T	G	100.0%	146.7 / NA	50	Q404P (CAG→CCG)	nuoN	NADH:quinone oxidoreductase subunit N
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/19);  total (31/19)

ATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCT  >  NC_000913/2390052‑2390534                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 aTCAGCGGCAGGGCCAACCGCACAATGCTAAGCAGCGGTTGGGGCCATCCACCCAGCCCCATTACCACCAGTGCAGAGATCAGCGCACC‑GATACCGCCCGCGCTGTACTGCCAGGTTGATGGGTCATCGCGCCCCGGTTGTTCCGGGGCGTGAAGATACGGGCGCACCGCCAC‑GCGGAGGTAGTA‑GTTGAGGCCGATTGCCGAACCGACAACCACGGCACCCGCCA‑GCCACCACAAGTGTGCCGGGACacc                                                                                                                                                                                                                                           <  2:370587/251‑1 (MQ=255)                    gCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCACCAGTGCAGAGATCAGAACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCAGGTTGTTCCCGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCAACCACAAGTGTGCCGGGACACCCACCGCCAGCACGTAGAAc                                                                                                                                                                                                                        >  1:250628/1‑251 (MQ=255)                              ctgcAGCGGTTGTGGCCATACCCCCAGCACCAGTACCAACAGTGCAGAGATCAGCACACC‑GATCCCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTGGTCCCGGGGGGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGGAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGAt                                                                                                                                                                                                                <  2:63491/246‑1 (MQ=255)                                tCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGAt                                                                                                                                                                                                                >  1:63491/1‑247 (MQ=255)                                tCAGCGGTTGCGGCCATACAAACAGCACCAGTACCAACAGTGCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTACTGCCCGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGAAACCGGCCCACCGCCCC‑GCGCCGGTAGTA‑GTAGCGGCCGATTGCCGAACCGACCACCACGGCACACACCA‑ACCACCACACCAGGGCCGGGACCCCGACCGCCAGCACACAGGACTTACCGATacac                                                                                                                                                                                                            >  2:195042/1‑248 (MQ=255)                                             ccATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGC‑ACAGCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCCCCCAACA‑GCCACCACAAGTGGGCAGGGCCACCGACCGCCAGCACGTAGCACTTCCCGCTCAAGCCCCGCGTCATCg                                                                                                                                                                                               >  2:41755/1‑251 (MQ=255)                                                 ccccccAGCCCCAGTCCCAACAGGGCAGAGATCAGC‑CAACCAATACCCCCCTGTCTGTCCCGCCAGTTTGATGTTGCATCGCGCCCCGTTTGTTCCGGGGGGTGAAGATACAGGCTCCCCGCCAC‑GCGCAGGT‑GTAAGTATAGGCCTATTGCCGACCCGACACCCACGGCACCCACCA‑GCCACCACAAGTTTGCCGGGCCACCGTCCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGAt                                                                                                                                                                                           <  2:301698/248‑1 (MQ=255)                                                        gCACCAGTACCAACAGTGCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGcc                                                                                                                                                                                    >  1:9135/1‑251 (MQ=255)                                                           ccAGTACCAACAGTGCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTACGGCCAGTTTGATGGTGCATCGCGACCCGGTTGTCCCGGGGCGTGAAGATACAGGCCCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGTGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCaga                                                                                                                                                                                 <  1:189269/251‑1 (MQ=255)                                                             aGTACCAACAGTGCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCag                                                                                                                                                                                  >  1:327498/1‑248 (MQ=255)                                                             aGTACCAACAGTGCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGAAGAACCTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCag                                                                                                                                                                                  >  2:85018/1‑248 (MQ=255)                                                             aGTACCAACAGTCCAGAGAACAGCACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGCCCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGACCCGACAACCACGGCACCCACCA‑GCCACCACAAGGGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAgaga                                                                                                                                                                               <  1:352555/251‑1 (MQ=255)                                                              gTACCAACAGTGCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTAATGCCAGTTTGATGGTGCCTCGCGACCCGGATGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑CCCCAGGGAGTA‑GTAGAGGCCGATTGCCGAACCGCCAACCACGGAACCCACCA‑GCCTCCACACGTGCGCCGGGACACCTAACGCCCGCACGCAGCACCTACCGACAAAGCCCAGCCGCATCGGGACACCCGCCCGAGAc                                                                                                                                                                              >  2:193026/1‑251 (MQ=255)                                                                     cagggCAGAGATCAGCACAAC‑AATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGCCCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACACCCACGGCACCCCCCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatca                                                                                                                                                                       <  2:116371/247‑1 (MQ=255)                                                                         gCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTACAGCCAGTTTGATGGGGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGGA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGGCAGCACGTCGAACTTACCGTGAAAGCCCAGCGGCATCGGGATCCCGGCCAGAGCCAGCATCCTCAc                                                                                                                                                                   >  2:314514/1‑251 (MQ=255)                                                                            gagaTCAGCACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGAGAAGATACAGGCTCACCGCAAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCgg                                                                                                                                                                >  1:216836/1‑250 (MQ=255)                                                                             cgaTCAGCACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCCTCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCCCAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTCCCCGATCCAGCCCAGCGTCAACGGGAAACCGGCCAGAGACAGcatcctccacgtc                                                                                                                                                               >  2:60333/2‑242 (MQ=255)                                                                             agaTCAGCACAAC‑GATACCGCCCGCGTTGTACTGCCAGTTTGATGGTGCATCGCGACCCGTTTGTCCGGGGGCGGAAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑TTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGGGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGtc                                                                                                                                                               <  1:213350/251‑1 (MQ=255)                                                                                                 gcccgcGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCCTCACTGCCGCGAG‑GATCg                                                                                                                                           >  1:369533/1‑251 (MQ=255)                                                                                                  cccgcGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCgg                                                                                                                                          >  2:345471/1‑251 (MQ=255)                                                                                                    cgcgCTTTACGGCCAGTTGGAGGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCCCCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGAc                                                                                                                                        <  1:161181/251‑1 (MQ=255)                                                                                                    cgcgCTGTACTGCCAGTTTGCTGGTGCATCGCGACCCGGTTGTTCCGGCGCGTGACGATACAGGCTCACCGCCCC‑GCGCAGGTAGTA‑GTCGCGGCCGCTTGCCGAACCGACAACCACGGCACCCACCA‑GCCCCCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGAc                                                                                                                                        >  2:31944/1‑251 (MQ=255)                                                                                                    cgcgCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGAc                                                                                                                                        >  1:371311/1‑251 (MQ=255)                                                                                                            aCTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGGAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGACCTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCACCACCGTCATCACTGCCGCGAG‑GATCGGACGCTGcc                                                                                                                                  >  2:358042/1‑249 (MQ=255)                                                                                                                        aTGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCAAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCgg                                                                                                                    >  1:9563/1‑251 (MQ=255)                                                                                                                              cATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAag                                                                                                                >  1:309573/1‑249 (MQ=255)                                                                                                                                    gccccGGTTGTTCCGGGGCGTGAAGATACAGGCCCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCCCCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAgg                                                                                                        <  2:9135/249‑1 (MQ=255)                                                                                                                                         ggTTGTTCCGGGGCGTGAAGATACAGGCTCCCCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCCCCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATc                                                                                                   <  1:424154/251‑1 (MQ=255)                                                                                                                                             gttCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGACTCATCACCGTCATCACTGCCGCGAGTGA‑CGGACGATGCCCGAACAAACCGCGGGAAGAGAAAAGGGAATCAGcc                                                                                               >  2:80350/1‑250 (MQ=255)                                                                                                                                                cggggggggaatgatccagcccccccGCCAC‑CCGCGGGTAGTA‑GAAGAGGCCGATGGCCGAACCGCCAACCCCGGCCCCCCCCA‑TCCACCACAAGTGTGCCGGAACGCCGGCCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATcc                                                                                            <  2:213618/227‑1 (MQ=255)                                                                                                                                                      cGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACCCCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GAACGGACGCTGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGcc                                                                                       >  1:275594/1‑250 (MQ=255)                                                                                                                                                                  ggCTCACCGCCAC‑GCGAAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTg                                                                          >  1:100932/1‑251 (MQ=255)                                                                                                                                                                              c‑gcgCAGGTAGTA‑GTAGAGGCCGGTTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAATTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCAGCACTGCCGCGAG‑GATCGGACGATGCCAGGACAGTCCGCGGTAAGAGGACAGGGAATCAGCATCCGGGGCACGATACGTGCTGGACATCAGGCTg                                                              <  1:154195/251‑1 (MQ=255)                                                                                                                                                                                 cgcagggcgga‑gtagAGGCCGATTGCCGAACCAACAACCGCGGCACCCCCCA‑TCCCCCCCAAGTGTGCCGGGACCCCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGAc                                                            <  1:299796/241‑1 (MQ=255)                                                                                                                                                                                  gcAGGTAGTA‑GTAGAGGCCTATGGCCGAACCGACACCCCCGGCCCCCACCC‑CCCGCCTCAGGTGTGCCGGGACACCGTCCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACACCAGCATCTCCGTCATCACTGCCGCGGG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGTAATCAGCATCCGGGCTACGATACGGGCTGGACATCAGGCTGAcc                                                           <  1:205907/251‑1 (MQ=255)                                                                                                                                                                                     ggTAGTA‑GTAGAGGCAGATTGCCGAACAGACAACCCCGGCAGCCACCA‑GCCACCACAAGAGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATACAGCCCAGCGGCCTCGGGCTACCGGCCAGAAACAGCATAATCACCGGCATCACTGCCGCGAG‑GATCGGCCGATGCCGGTACAGACCGCGGTAAGAGAACAGGGACTCGGCATCCCGGCTACGCGAGGGGCTGGACATCAGGCTGAcaccc                                                        >  2:29393/1‑247 (MQ=255)                                                                                                                                                                                        agta‑gtagAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCg                                                     >  2:123998/1‑251 (MQ=255)                                                                                                                                                                                            agaagagGCCGATTGCCGAACCGAAAACCACGGCCCCCACCA‑GCCACCACAAGGGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAc                                                  <  1:59790/248‑1 (MQ=255)                                                                                                                                                                                               agagGCCGCTTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACAACGCCGAAcgcg                                               >  1:115949/1‑251 (MQ=255)                                                                                                                                                                                                  ggCCGATTGCCGAACCGACAACCACGGCACCCCCCA‑GCCCCCAAAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCg                                            <  1:120399/251‑1 (MQ=255)                                                                                                                                                                                                           ccgaaccgaaACCCCCGGCACCCCCCA‑GCCACCAAAAGTGCGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGctgctg                                   <  1:83590/251‑1 (MQ=255)                                                                                                                                                                                                                ccGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGCCCACGCCGAACGCGCCGAGGCTGCTGAAc                                >  2:83001/1‑249 (MQ=255)                                                                                                                                                                                                                    cAACCACGGCACC‑ACCAAGCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGacaagata                           >  2:409786/1‑243 (MQ=255)                                                                                                                                                                                                                     aaCCACGGCACCCACCA‑TCCAACAAACGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACAGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGCACAGACCGCGGTAAGCGAACAGGGCATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGACGAGGCTGCTGAACAGATAAc                         >  2:409429/1‑251 (MQ=255)                                                                                                                                                                                                                       ccACGGCACCCACCA‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCg                       >  1:44044/1‑251 (MQ=255)                                                                                                                                                                                                                              acccaccA‑GCCACCACAAGTGTGCCGGGACACCGACAGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGt                >  1:256452/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccca‑GCCACCACAAGTGTGCCGGGCCCCCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAc            <  1:387033/250‑1 (MQ=255)                                                                                                                                                                                                                                  acca‑GCCACCACAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAc            >  2:206735/1‑251 (MQ=255)                                                                                                                                                                                                                                          accacAAGTGTGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGCCGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTAcc     >  1:185577/1‑251 (MQ=255)                                                                                                                                                                                                                                             acaAGTGGGCCGGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCt  <  1:232236/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAC‑GATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTCACCGCCAC‑GCGCAGGTAGTA‑GTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCA‑GCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAG‑GATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCT  >  NC_000913/2390052‑2390534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑