Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,389,974	T→G	intergenic (+10/+74)	yfbP → / ← nuoN	uncharacterized protein YfbP/NADH:quinone oxidoreductase subunit N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,389,974	0	T	G	100.0%	129.0 / NA	43	intergenic (+10/+74)	yfbP/nuoN	uncharacterized protein YfbP/NADH:quinone oxidoreductase subunit N
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/17);  total (26/17)

TTTGGAGGAACAAAGGAAAATAATAATTACCCCTTGATGCAGATGTGTGTTGATACTTATTTTGCGCAGAGAAAACCGCTTCAGGCATTAACCCTGTTACATAATTATGCCTGGATAATGTCCAGTGAAACGACTGCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTC  >  NC_000913/2389728‑2390216                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   atcaccaatatccgtatgcaggcagtcggtttggatctgccgctccagacgcgctccaaccagatcccgtggatcaacacttggctggtgtctgataacgtgcaggttgctacgcaggaagtggaagtcagttcttatctggtagggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacaccaTTAACGCTCTGCGgctat                                                                                                                                                                                                                                                >  1:66329‑M2/234‑251 (MQ=255)          atccgtatgcaggcagtcggtttggatctgccgttccagacgcgctccaacccgatcccgtggatcaacacttggctggtgtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATcc                                                                                                                                                                                                                                       <  1:226724‑M2/27‑1 (MQ=255)          atccgtatgcaggcagtcggtttggatctgccgttccagacgcgctccaacccgatcccgtggatcaacacttggctggtgtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttacccggcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATc                                                                                                                                                                                                                                        <  1:409123‑M2/26‑1 (MQ=255)                        agtcggtttggatctgccgtaccagcagcgatccaacccgatcccgtggaacaatacatggctggcgtctgataacgtgccgcttgctccgcaggaagaggacgtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacacTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCagc                                                                                                                                                                                                                          >  2:189269‑M2/211‑249 (MQ=255)                                 ggatctgccgttccagacgcgctccaacccgatcccgtggatcaacacttggctggtgtctgataacgtgcaggttgctccgcaggaagtggaagtaagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcccaccaTTAACGCTCTGCGGCTCTCTACTATCCCTTCTTTTTCAGACAAAccgccg                                                                                                                                                                                                                >  2:142349‑M2/202‑251 (MQ=255)                                     ctgccgttccagacgcgctccaacccgatcccgtggatcaacacttggctggtgtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGgctatct                                                                                                                                                                                                                                              <  2:120968‑M2/20‑1 (MQ=255)                                     ctgccgttccagacgcgctccaacccgatcccgtggatcaacacttggctggtgtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGgctatct                                                                                                                                                                                                                                              >  1:120968‑M2/198‑217 (MQ=255)                                     ctgccgttccagacgcgctccaacccgatcccgtggatcaacacttggctggtgtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGc                                                                                                                                                                                                             >  2:403122‑M2/198‑250 (MQ=255)                                            tccagacgcgctccaacccgatcccgtggatcaacacttggctggtgtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTCCTATCCCTTTTTTTTCAGAAAAACCGCCGAGCATGTcggc                                                                                                                                                                                                     >  2:331231‑M2/191‑251 (MQ=255)                                                gacgcgctccaacccgatcccgtggatcaacacttggctggtgtctgataaagtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCt                                                                                                                                                                                                 >  1:65037‑M2/187‑251 (MQ=255)                                                            cccgatcccgtggatcaacacttggctggtgtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagatcgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTAc                                                                                                                                                                                      >  1:222411‑M2/175‑250 (MQ=255)                                                              cgatcccgtggatcaacacttggctggggtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAACAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                                                                                    >  2:352060‑M2/173‑250 (MQ=255)                                                                   ccgtggataaacacgtggcgggtgtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgagttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAAc                                                                                                                                                                              <  2:280760‑M2/84‑1 (MQ=255)                                                                     gtggatcaccacttggctggtgtctgataacgtgcaggttgccccgcaggaagtggaagtcagttcttatctgtgcgggcagattgactcggaagtggaccccgacgatttgagtaacttccagctctgatgtcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCGGACTTACAAAGTAACAg                                                                                                                                                                            <  1:308103‑M2/86‑1 (MQ=255)                                                                        gatcaacacttggctggtgtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGAtt                                                                                                                                                                         <  1:28671‑M2/89‑1 (MQ=255)                                                                           caatacttggctggtgtgtgatcacgggcaggtagctccgcaggaagtggaagtcagtgcttatctggtcgggcagattgactcggaagtggacacctgcgatttgagaaacttccagctctgatggcccgcgttaccctgcgaatcactggctcacaaTAAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACa                                                                                                                                                                      <  2:216110‑M2/92‑1 (MQ=255)                                                                                cttggctggtgtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGc                                                                                                                                                                 >  1:1286‑M2/155‑251 (MQ=255)                                                                                          gtctgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGcc                                                                                                                                                        <  1:403122‑M2/106‑1 (MQ=255)                                                                                             tgataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAAc                                                                                                                                                    >  1:294856‑M2/142‑251 (MQ=255)                                                                                              gataacgtgcaggttgctccgcaggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAAc                                                                                                                                                    <  1:260221‑M2/110‑1 (MQ=255)                                                                                                       caggttgctccgcaggaagtggaagtcagttcttctctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCt                                                                                                                                          >  2:70263‑M2/132‑251 (MQ=255)                                                                                                          gttgatccgcaggaagtggaagtcagttctgatctggtcgggcagattgactcggaagtggaccccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                                                                                       <  2:148063‑M2/123‑1 (MQ=255)                                                                                                                 cgcaggaagtagaagtcagttcttatctggtcgggcagattgactcggaagcgggcaccgacgctttgagtaacttccacctctgatggcccgcgttaccctgcgcatcactggaacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGt                                                                                                                                >  1:42536‑M2/122‑251 (MQ=255)                                                                                                                     ggaagtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgtg                                                                                                                            <  1:70263‑M2/134‑1 (MQ=255)                                                                                                                       aagtggaagtcagttcttatctggtcgggccgattgactcggaagtggacaccgacgatttgagtcacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGCAAAACCGCCGAGCATGTCGGCGGCTTTCTGCCTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATCCTAATCAGCGGATGTGGc                                                                                                                          >  2:59790‑M2/116‑251 (MQ=255)                                                                                                                        agtggaagtcagttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGcc                                                                                                                         >  1:213618‑M2/115‑251 (MQ=255)                                                                                                                                    ttcttatctggtcgggcagattgactcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGc                                                                                                              >  2:83590‑M2/103‑250 (MQ=255)                                                                                                                                                 ggcgagttggactcggaagtggaccccgacgatttgagtaacttccagcgcagatggcccgcgttacgctgcacatcactggcacacaaTTATCGCTCTGCGGCTATCTGCTATCCCTTCTTTTTCAGAAAAGCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAACAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAg                                                                                                <  2:109006‑M2/162‑1 (MQ=255)                                                                                                                                                     agattgactcggaagtggacaccgacgatttgagtaacttccagctctgatgccccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTa                                                                                                                                         >  1:325115‑M2/86‑206 (MQ=255)                                                                                                                                                     agattgactcggaaggggacaccgacgatttgagtaccttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                                                                            <  1:374133‑M2/166‑1 (MQ=255)                                                                                                                                                           actcggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatccctggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATc                                                                                      <  2:294856‑M2/172‑1 (MQ=255)                                                                                                                                                              cggaagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCGCCAGTACCAACAGTGCAGAGATCAGc                                                                                   <  1:123827‑M2/175‑1 (MQ=255)                                                                                                                                                                 aagtggacaccgacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGcac                                                                                 >  1:230374‑M2/74‑250 (MQ=255)                                                                                                                                                                            gacgatttgagtaacttccagctctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGAGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgcc                                                                     >  1:301698‑M2/63‑251 (MQ=255)                                                                                                                                                                             ccgatttgagtaccttccagctctggtggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCGGCGGCTATCTCCTACCCCTTCTTTTTCAAAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgccc                                                                    <  2:25409‑M2/190‑1 (MQ=255)                                                                                                                                                                                                tgtctacgggcccgcgttccccctcccatcacgggcatacaaTTAACGCTCTGCGGCTATCGACTATCCCTTCTTTTACAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGtt                                                   <  2:119993‑M2/207‑1 (MQ=255)                                                                                                                                                                                                  tctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGtt                                                   >  1:119993‑M2/41‑247 (MQ=255)                                                                                                                                                                                                   ctgatggcccgcgttaccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACACCGATACCGCCCGCGCTGTACTGCCAGTTTGATg                                              >  2:81414‑M2/40‑251 (MQ=255)                                                                                                                                                                                                      atggcccgcgttcccctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATTCCGCCCGCGCTGTACTGCCAGTTTGATGGTg                                           <  2:23746‑M2/215‑1 (MQ=255)                                                                                                                                                                                                                  accctgcgcatcactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGCTCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCg                               >  1:260044‑M2/25‑251 (MQ=255)                                                                                                                                                                                                                      tgcgcataactggcacacaaTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGGCGGCGGCTTTCTGCCTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACTCCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGGCAGTTTGATGGTGCATCGCGCCCCGgttg                           >  2:187774‑M2/21‑251 (MQ=255)                                                                                                                                                                                                                                    acacACTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAAAAACACCGCCGAGCATGTCGGCAGCTTTCTAACTTACAATGTAACAGCTTACATCAGCGGCATTGCCAAACGCACAATCATAATCAGCGGTTGTGGCCATACACCCCGCACCAGTACCAACAGTGCAGAGATCAGCACACCGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGa              >  2:213350/4‑250 (MQ=255)                                                                                                                                                                                                                                         aTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATAc        >  1:116371‑M2/2‑251 (MQ=255)                                                                                                                                                                                                                                             aCGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGc    >  1:98399/1‑251 (MQ=255)                                                                                                                                                                                                                                                ctctGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTc  >  1:351779/1‑250 (MQ=255)                                                                                                                                                                                                                                                ctctGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATAACGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGAGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTc  >  1:200607/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   TTTGGAGGAACAAAGGAAAATAATAATTACCCCTTGATGCAGATGTGTGTTGATACTTATTTTGCGCAGAGAAAACCGCTTCAGGCATTAACCCTGTTACATAATTATGCCTGGATAATGTCCAGTGAAACGACTGCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCAGTTTGATGGTGCATCGCGACCCGGTTGTTCCGGGGCGTGAAGATACAGGCTC  >  NC_000913/2389728‑2390216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑