Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,311,650	A→G	F367S (TTC→TCC)	ompC ←	outer membrane porin C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,311,650	0	A	G	95.9%	158.4 / NA	49	F367S (TTC→TCC)	ompC	outer membrane porin C
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (27/20);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

ATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGG  >  NC_000913/2311410‑2311892                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    aTAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAAc                                                                                                                                                                                                                                           >  1:125503/1‑251 (MQ=255)    gTTTCGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTGGGCTGGTGGTGGTTTGTGATCGCAGCCAACAAATAAGAGCGTGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTGCATACTTGATTATCCGCATGCGGGCGGTCGCAAGATTACACCAAAAATCGGAGTGTGTACGGTTAAAACAATGAAAAAAGGGCCCGTGGGGCCTTGGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAg                                                                                                                                                                                                                                        <  1:193694/251‑1 (MQ=255)                                    gTGGGTCTTGGAGGGTTGAGGTCTTTGATCGGCACCAACCCAGACAAGAGCCGCAGCCCCAAGTGCCACGCGGGTCCAGCTTAATTCAATACGTGATTATTCTCATGCGTAAGGGCGCAAAAGTACACCAAAAAATTGAGTTTGGACGCTGACACCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTTGATATCAATAGAGAATA‑AGACAGGAAAACCAGACCCAGAGCTATGCTGTTACAAGTGTTCATGc                                                                                                                                                                                                        >  2:276508/1‑251 (MQ=255)                                                           tttGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                                                                                                                                                                  <  1:137838/250‑1 (MQ=255)                                                             tGATCGCAAACAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtc                                                                                                                                                                               >  1:124735/1‑251 (MQ=255)                                                               atcgcaacccaccccgaaaagtgcgGCAACCAGCAGTGACACGCGGGTCCAGACTATTTCCATACGTGATTATCATCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGCCTGGTAAACCAGACACAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                                                                                                                                                             >  2:407916/23‑251 (MQ=255)                                                                        aacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                                                                                                                                                                    >  1:91773/1‑251 (MQ=255)                                                                              gaagaGCGCGGCAGCCAGCAGTGCCACGTGGGTAAAGCTTATTTCCATACGTGCTTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                                                                                                                                                              >  2:402484/1‑251 (MQ=255)                                                                                 gagCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGt                                                                                                                                                           <  2:358374/251‑1 (MQ=255)                                                                                       ggCAGCCAGCAGCGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGGTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCa                                                                                                                                                      >  1:332322/1‑250 (MQ=255)                                                                                             cagcagTGCCACGCGGGTAAAGCTTATTTCCATACGCGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGCGCTTGTACGCTGAAAACAATGAAAAAAGCGCCCCCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCCACGTAgg                                                                                                                                               >  1:65995/1‑251 (MQ=255)                                                                                             cagcagTGCCACGCGGGTAAAGCTTATGTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAGCTGGTTGTCGTCCAGCAGGTTGAGTTGGTAGTCAACGTAgg                                                                                                                                               <  2:65982/251‑1 (MQ=255)                                                                                                     ccACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑g                                                                                                                                      >  2:330815/1‑251 (MQ=255)                                                                                                          cgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑Gttttt                                                                                                                                 <  2:238976/251‑1 (MQ=255)                                                                                                               aaaGCGTATTTCCATACGTGATTAGCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTTAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCTATATCAATCGATATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCATCGTCACGAGTGAGCTGGTTTTCGTCTATCAGGTTGATTTTGTAGTCAACTTAGGTGTACAT‑GTTTttgttg                                                                                                                             <  2:289690/250‑1 (MQ=255)                                                                                                                aaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACCT‑GTTTTTGTTGa                                                                                                                            >  2:143281/1‑250 (MQ=255)                                                                                                                    ttATTTCCATACGTGATTATCCTCATGCGCACGGTCGAAAGAGAACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCACAAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAagt                                                                                                                         >  1:391840/1‑249 (MQ=255)                                                                                                                     tATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAagtagt                                                                                                                      >  1:404323/1‑251 (MQ=255)                                                                                                                           cATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAgtaggtag                                                                                                                <  2:23105/251‑1 (MQ=255)                                                                                                                            aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGCAGGTGGACAT‑GTTTTTGTTGAAGTAGGAGGTAGc                                                                                                               >  1:427738/1‑251 (MQ=255)                                                                                                                                  gATTATCCTCATGCGAACGGTCGCACGAGTACACCAACAAAATGAGTTTGTACGCTGCAAACAATGAAAAAAGGGCACGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACCGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                                                                                                                                   >  2:187471/1‑210 (MQ=255)                                                                                                                                  gATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCACGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCTTCACGAGTGAGCCGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                                                                                                                                   <  1:187471/210‑1 (MQ=255)                                                                                                                                                    gatCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGat                                                                                       >  1:302838/3‑251 (MQ=255)                                                                                                                                                        gCAAGAGTACACTAAAAAACAGAGTTTGTACGCTGACAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGCTATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCGAGCGTCACGAGTGAACTGGTTGTCGTAAAGCAGGTTGATTTTGCAGTCAACGTAGGTGGA‑ATAGATTTTGTTGGAGTAGTCGGGTGCACCAACATCAACACATTTAAGGATATCt                                                                                   >  2:3030/1‑251 (MQ=255)                                                                                                                                                            gagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAAGCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTTTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt                                                                               <  1:379466/251‑1 (MQ=255)                                                                                                                                                                       aaaaCTGAGTTTGTACGCTGAAAACAATGCAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGcc                                                                     >  1:147483/1‑250 (MQ=255)                                                                                                                                                                                tttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAgg                                                           >  2:95118/1‑251 (MQ=255)                                                                                                                                                                                     aCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGTTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGtttt                                                       <  1:279236/250‑1 (MQ=255)                                                                                                                                                                                       gCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTAc                                                    <  1:45240/251‑1 (MQ=255)                                                                                                                                                                                        cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACcaacat                                                                                                       <  1:124295/199‑1 (MQ=255)                                                                                                                                                                                        cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACcaacat                                                                                                       >  2:124295/1‑199 (MQ=255)                                                                                                                                                                                          gAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTTAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTAcc                                                   <  2:87578/249‑1 (MQ=255)                                                                                                                                                                                              aCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCttt                                                >  1:162448/1‑248 (MQ=255)                                                                                                                                                                                                aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGGTATCAGTGTTGATGCCAGCGTCACGAGTGAAGTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTGGAAGTAGTAGGGAGCACCAACATCATCATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACt                                           <  2:288708/251‑1 (MQ=255)                                                                                                                                                                                                  agAAAAAAGGGCCCGCAGGCCCGTTGTTCGATATCAATCGAGATTA‑GGCCTGGTAAACCAGACCCAGATCTACGATGTTATCAGTGGTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGGAGGTTGATTTTGTAGTCAAGGTAGGTGGACAG‑GTTGTTGTGGAGGTAGTAGGTAGGACCAACATCAACATATTTCAGGATATCTGCGGCGTCGTAGCGACGTCGCTGGTGTTTGCCTTTAGACTGc                                         <  2:408476/250‑1 (MQ=255)                                                                                                                                                                                                         aGGGCACGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGGGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACACAGGTTTTTACCTTTAGACTGCAGGTagg                                  >  2:145574/1‑249 (MQ=255)                                                                                                                                                                                                          gggCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGc                                 >  2:222101/1‑251 (MQ=255)                                                                                                                                                                                                           ggCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGcc                                >  2:233008/1‑251 (MQ=255)                                                                                                                                                                                                              ccGCAGGCCCTTTGGTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGAGGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg                             <  2:124735/251‑1 (MQ=255)                                                                                                                                                                                                               cGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCAAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGCAGTAGTAGGTAGCACCACCATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAggg                            >  2:41918/1‑251 (MQ=255)                                                                                                                                                                                                                   ggCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgg                        >  1:321917/1‑251 (MQ=255)                                                                                                                                                                                                                    gCCCTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga                       <  1:402484/251‑1 (MQ=255)                                                                                                                                                                                                                      ccTTTGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg                     <  2:147483/251‑1 (MQ=255)                                                                                                                                                                                                                        tttGTTCGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGGAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGCAAGCCAGGGCCGGACGc                    >  2:335517/1‑250 (MQ=255)                                                                                                                                                                                                                              cGATATCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTTAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg              >  2:6742/1‑250 (MQ=255)                                                                                                                                                                                                                                atatCAATCGAGCCTA‑GAACTGGTCAAACAGACCCAGCGCTACGATGTTCTCAGTGTTGATGCCAGCGTCACGAGTGAAATGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAAAATAAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGa             >  2:25624/1‑249 (MQ=39)                                                                                                                                                                                                                                  atCAATCGAGATTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTAGCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGGCGTCGTAGCCACGACCCAGGTTTTTACCTGTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTc         <  2:306595/251‑1 (MQ=255)                                                                                                                                                                                                                                     aaTCGAGATTATGAA‑TGGTAAACCAGACCCCGACCTACGATGTTATCAGTGTTGCTGCAAGCGACACGAGTGAACTGGTTGTCGTCACGCCGGTTGATTTTGTAGTCAACGCAGGTGGCCAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATAAACATATTTCAGGATATCTTCGTCGTCGCAGCCACGACCCAGGTTTTTTCCCTTAGACTGCAGGTAAGCCAGGGACGGCCGCAGACCGATGTCGac      >  2:73211/1‑250 (MQ=255)                                                                                                                                                                                                                                         gagaTTA‑GGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg  <  2:302838/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    ATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTA‑GAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT‑GTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGG  >  NC_000913/2311410‑2311892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑