Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,225,170	G→A	F154F (TTC→TTT)	yohC ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,225,170	0	G	A	84.0%	117.0 / 14.5	50	F154F (TTC→TTT)	yohC	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base A (22/20);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

GATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTACGCGCCATCCACCAGATGACCCGCCCCATCACCGCGACCCCAGCCAGCATAACGC  >  NC_000913/2224925‑2225402                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         gATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCt                                                                                                                                                                                                                                     >  2:353866/1‑251 (MQ=255)  aTTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTc                                                                                                                                                                                                                                    <  1:141135/251‑1 (MQ=255)     tACTGATGCTCGAGACGCCAGCATAAGGAGCAAAAAGTGCGAGCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACGTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGATCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAATGCCTGCCTCTTTGTTGATATGCAGAAAGCTCgg                                                                                                                                                                                                                                  <  2:93104/250‑1 (MQ=255)         gAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGAAt                                                                                                                                                                                                                               <  2:137471/249‑1 (MQ=255)                 cGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTaa                                                                                                                                                                                                                     <  1:321382/251‑1 (MQ=255)                        atcatAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGt                                                                                                                                                                                                              <  1:47285/251‑1 (MQ=255)                          catAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGAATACCAAGGTAAAGCAGGTAg                                                                                                                                                                                                            <  2:403772/251‑1 (MQ=255)                             aaCGAGCAAAAAGTGCGATCGTCAAAGGAGACTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCg                                                                                                                                                                                                         >  2:383343/1‑251 (MQ=255)                                     aaaaGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGAAGGTAGCCGGTGTaaa                                                                                                                                                                                                  >  2:42257/1‑250 (MQ=255)                                           gcgctCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGGACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCGTCCTCTTGGTTGATATGCAGGAAGCTCGGAATACCAAGTTAAAGCAGGGAGCCGGTGTAAAACAgcg                                                                                                                                                                                            <  2:268672/246‑1 (MQ=255)                                            cGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGcc                                                                                                                                                                                          <  1:106535/251‑1 (MQ=255)                                              aTCGTCAAAGGCGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCAc                                                                                                                                                                                        >  1:53051/1‑251 (MQ=255)                                                 gTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCATGGAGTCGGGGTAAAACATCTCCACCGt                                                                                                                                                                                     <  1:334749/251‑1 (MQ=255)                                                 gTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGt                                                                                                                                                                                     >  1:45252/1‑251 (MQ=255)                                                         agaATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGAcc                                                                                                                                                                              >  1:341219/1‑250 (MQ=255)                                                                      aaTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAacc                                                                                                                                                                 >  1:26429/1‑250 (MQ=255)                                                                            gcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCTGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAaccagacc                                                                                                                                                            <  1:385453/249‑1 (MQ=255)                                                                              aTTGCTGACATTTACGCCAGCAATGCAACGGCAAAGAACTTTCTAGAACAAACGATAACCGTAGACCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTg                                                                                                                                                        <  2:81345/251‑1 (MQ=255)                                                                                 gCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTATAGAACACACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGAAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGAt                                                                                                                                                     >  1:269596/1‑251 (MQ=255)                                                                                              ccAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATTACGGTTAGGGCGATCAGCACGTCCATTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCCTCCTCTTTGTTGATATTCAGAAAGCGCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGGGCCGGGCAGCGCACACAACCAGAACAGTGGATAAAGCGCCACCAg                                                                                                                                        <  1:432744/251‑1 (MQ=255)                                                                                               cAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGccacc                                                                                                                                          >  1:156989/1‑248 (MQ=255)                                                                                                   aaTGCAGCGTCAGAGAACTTTCTAGAACAACCGATAACCGTATCCCAATACTATCACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACCCCAATCGCCAGCGTCGACCTAGAGACGCTAAGGCCTCCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAACGCGCCACCAGACCGc                                                                                                                                   >  2:155086/1‑251 (MQ=255)                                                                                                   aaTGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGCGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGc                                                                                                                                   >  1:54643/1‑251 (MQ=255)                                                                                                    aTGCAACGTCAAAGAACTTTCTAGAACAAACGATAACAGTAGCCACATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTAACAGAACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGACCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCACTGGATAAAGCGCCACCAGCCCGCt                                                                                                                                  >  1:205282/1‑251 (MQ=255)                                                                                                           gTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATCGTATAACGGTTAGGGCGAGAAGCACTTCCAGTACCAGCACGCCACTCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATAACAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAAc                                                                                                                           >  2:405563/1‑251 (MQ=255)                                                                                                           gTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAACACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAAc                                                                                                                           <  1:237503/251‑1 (MQ=255)                                                                                                                       tCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGc                                                                                                                <  1:26356/250‑1 (MQ=255)                                                                                                                         tAGAACAACCGATCACCGTCGCCCCATAGTATAACGGTTGGGGCGAGCAGCACTTCCAGTACCAGCACTCCAATCGCCAGCGTCGAACTAGCGGAGCTAAGGCCTTCCTCTTTGCTGATCTTCAGAAAGCTAGGACTACCAAGGTAAAGCAGGAAGCCGGTGCAACACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGAGGACAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTGGCAAc                                                                                                             >  2:289894/1‑251 (MQ=255)                                                                                                                           gAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGt                                                                                                           <  1:283994/251‑1 (MQ=255)                                                                                                                           gAACAAACGATAACAGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGTCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGCTAAAGAGCCACCAGACCGCTTAAAAACAGCGGAGTGGCAACGt                                                                                                           >  2:290202/1‑251 (MQ=255)                                                                                                                            aaCAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGt                                                                                                           >  2:326528/1‑250 (MQ=255)                                                                                                                                aaCGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGTTGTAAAACAGCTCCACCGTGCCGACCATCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGGTGCAACGTAGccc                                                                                                      <  1:206282/251‑1 (MQ=255)                                                                                                                                                    aGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTgcgc                                                                                  >  1:31437/1‑251 (MQ=255)                                                                                                                                                                 gggcgtgaaaatcTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGACTTCCTATTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTCGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACCACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAACACAGCGGAGTTGCACCGTAGCCCGCGACGACCAGGCAGTGCGCTAGTGACGGACGc                                                                     >  2:387391/13‑251 (MQ=255)                                                                                                                                                                     gAGCAGCACTTCAAGTACCAGCAGGCCAATCGCCCGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGATGATCTTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGCGCCGACCAGCGCACACAACCCGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCg                                                                 >  2:274369/1‑251 (MQ=255)                                                                                                                                                                         agcaCTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATa                                                             <  1:42257/251‑1 (MQ=255)                                                                                                                                                                                ccAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTAcgc                                                      <  1:168132/251‑1 (MQ=255)                                                                                                                                                                                 cAGTACCAGCACGCCAATCGCAAGCGTCGCACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTAcgcg                                                     >  2:71130/1‑251 (MQ=255)                                                                                                                                                                                      ccAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTACGCGCCATc                                                >  1:423900/1‑251 (MQ=255)                                                                                                                                                                                            cGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTACGCGCCATccacc                                            >  2:144726/1‑249 (MQ=255)                                                                                                                                                                                              ccAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGAGGCTGCGGAGAATTACGCGCTATCCGCCAGAt                                        <  2:269596/251‑1 (MQ=255)                                                                                                                                                                                              ccAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGGGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTACGCGCCATCCACCAGAt                                        <  1:405563/251‑1 (MQ=255)                                                                                                                                                                                               cAATCGCCAGAGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAATTTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCCGTGGATAAAGCGCCACCCGACCGCTTCACAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTCCGCAAGTGACGGACGCTGCGGATAATTACGCGCCACCCACCAgcgg                                       >  2:169505/1‑248 (MQ=255)                                                                                                                                                                                                 aTCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGGCCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGTAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGt                                                                              <  2:152689/210‑1 (MQ=255)                                                                                                                                                                                                 aTCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGCAGACCATGCAGTGCGCAAgc                                                                              >  1:152689/1‑209 (MQ=255)                                                                                                                                                                                                          gTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTACGCGCCATCCACCAGATGACCCGCCCCAt                            <  1:353866/251‑1 (MQ=255)                                                                                                                                                                                                                 tAGAGAAGCTAAGGCATTCCTCTTTGTTGATATTAAGGAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCTCCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACAAGACCGCTTAAAAACAGCGTAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGAGCAAGTGACAGACGCTGCGGATAATTACGCGCCAGACACCAGATGACCCGCACCATCACcgcg                     >  2:311575/1‑251 (MQ=255)                                                                                                                                                                                                                      aaGCTAAAGCTTTCCTCTTTCTTGATATTCATAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTCAAACAGCGCCACCGTGACGACCAGCGCACACACCCAGACCAGTGGATAAAGCGCCACCAGACCGCTTACCACCAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTACGCGCCATCCACCAGATGACCCGCCCCATCCCCGCGAcccc                >  2:364768/1‑251 (MQ=255)                                                                                                                                                                                                                       aGCTAAGGCCTTCCTCTTTGTTGATATTCAGAAAGCTCGGAAGACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCCCCAGACCGCTTAAAACCAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTACGCGCCATCCACCAGATGACCCGCCCCATCACCGCGACCcca               <  2:45252/251‑1 (MQ=255)                                                                                                                                                                                                                                    cgctttggttattttCAGAAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGGGGATAAAGCGCCCCCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTACGCGCCATCCACCAGATGACCCGCCCCATCGCCGCGACCCCAGCCAGCATAACGc  <  2:53051/238‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         GATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGAATACCAAGGTAAAGCAGGTAGCCGGTGTAAAACAGCGCCACCGTGCCGACCAGCGCACACAACCAGACCAGTGGATAAAGCGCCACCAGACCGCTTAAAAACAGCGGAGTTGCAACGTAGCCCGCGAAGACCATGCAGTGCGCAAGTGACGGACGCTGCGGATAATTACGCGCCATCCACCAGATGACCCGCCCCATCACCGCGACCCCAGCCAGCATAACGC  >  NC_000913/2224925‑2225402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑