Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	97.5%	133.2 / ‑6.4	40	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (20/19);  minor base C (1/0);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCG  >  NC_000913/1905522‑1905992                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        cAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTcc                                                                                                                                                                                                                              >  1:145860/1‑251 (MQ=255)  aaaaCGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTct                                                                                                                                                                                                                              >  1:420179/1‑249 (MQ=255)           cACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGa                                                                                                                                                                                                                     >  2:159480/1‑250 (MQ=255)                       gAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                                                                                                                                                                                                        <  2:330374/251‑1 (MQ=255)                                 agtcagGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATGTTCTGAAGCATTGCGAAcc                                                                                                                                                                                              <  1:337842/251‑1 (MQ=255)                                  gtcagGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAACCCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCGTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCg                                                                                                                                                                                             <  2:49807/251‑1 (MQ=255)                                  gtcagGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCg                                                                                                                                                                                             <  2:105582/251‑1 (MQ=255)                                  gtcagGCGAGAACATATGCACATCAATCGCTATGCCTGCTTGATCGCGAAATCCATCACGGCTTGTTGTTCATGTAGGCATGTTTGCTGGGTGCGTGGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGGTCTTCTTGCGTTTGGTATGTAGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCg                                                                                                                                                                                             <  2:361806/251‑1 (MQ=255)                                    cagGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                                                                                                                                                                                           >  1:70462/1‑251 (MQ=255)                                      gtcgtgaCCATATGCACATCACTCGCTATGCCTGCATGAACGCGAAAACCATAACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGCTACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAAT‑GGTGAAAGATGCCACCCTCCATAAATCGAAATTTTCTGAAGCATTGCGAACCGGCCt                                                                                                                                                                                         >  2:304671/6‑251 (MQ=255)                                                  tGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATAACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGc                                                                                                                                                                             >  2:344305/1‑251 (MQ=255)                                                      caTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTTCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTATGAAGCATTGCGAACCGGCCTTATTTTTGGTGGCGTc                                                                                                                                                                         >  2:30790/1‑251 (MQ=255)                                                                                cgAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATTGATACCCCGGTCAGGACAGTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAATCCCTGACGCCGCTGATCGGCTg                                                                                                                                               <  1:362304/251‑1 (MQ=255)                                                                                   aaaCCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTgggg                                                                                                                                            <  1:168739/251‑1 (MQ=255)                                                                                      ccATCGCGGCTTGTTGTTCATGTAGGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATCCCTCGGTCAGGACATTGTCATGAATATCACTTCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGAGGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCTAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAAt                                                                                                                                         <  1:185422/251‑1 (MQ=255)                                                                                                    tgttCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGATACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagccc                                                                                                                           >  1:92046/1‑250 (MQ=255)                                                                                                           gTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGtt                                                                                                                    <  1:237955/251‑1 (MQ=255)                                                                                                           gTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                                                                                                     <  1:401053/250‑1 (MQ=255)                                                                                                                 aTGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATGGGCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGGCGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCt                                                                                                              <  2:382334/251‑1 (MQ=255)                                                                                                                   gTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTg                                                                                                            <  2:310013/251‑1 (MQ=255)                                                                                                                            gggCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAAcc                                                                                                   >  1:80794/1‑251 (MQ=255)                                                                                                                              gCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGACGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACCCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGGATGGAACCAc                                                                                                 >  1:78683/1‑251 (MQ=255)                                                                                                                                          tttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGttt                                                                                     >  2:406384/1‑251 (MQ=255)                                                                                                                                                    ccccGGTCACGACATTGTCATGAATTTCCCTGCAACTGTTCTTCTTGCGATTGGTATGTAGATGGATGACTTTGCTGCTTCAATCGGT‑ACAGATGCCACCCTCCATAAACCGACATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTCGTGCCGTCGACACCATGACGCCGCTGCTCGGCTGGGGAATGGGCCTGTTAGCCACCCGGTTTGTCCTTGCATGGCACCCCTGGATTGCGTTTGTGctgctg                                                                            >  2:36830/1‑250 (MQ=255)                                                                                                                                                        ggTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATAtt                                                                       <  1:159480/251‑1 (MQ=255)                                                                                                                                                           cAGGACATTGTCATGAATATCCCTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AACGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGCTTGTCCTTGCATGGTACCACTGGATTGCGTTTGTGCTGCTGATATTctc                                                                    >  2:215243/1‑249 (MQ=255)                                                                                                                                                            aGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTc                                                                   <  1:117334/251‑1 (MQ=255)                                                                                                                                                              gACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTcgg                                                                 >  1:125927/1‑251 (MQ=255)                                                                                                                                                                        tGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGCATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATg                                                       >  2:314741/1‑251 (MQ=255)                                                                                                                                                                            tatCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGCATGGGCATGTTGGCCAGCCGGTTTGTCCTTGAACGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGGGaaagtttt                                                   >  2:338999/1‑245 (MQ=255)                                                                                                                                                                               cACTGCTACTTTTCTTCTTGCGTTTGGTATGGCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTg                                                <  2:338808/251‑1 (MQ=255)                                                                                                                                                                                                 tGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGACTGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTTTTGTGGGTTTTCGTGgcgc                                >  1:351571/1‑249 (MQ=255)                                                                                                                                                                                                   cGTTTGGTATGTCGATGGAGGCATGTGCTGCATCAAGCGGT‑AAAGATGCCACCCTCCATAAACCGAAATGTTCGGAAGCATTGCGAACCGGCCTTAGTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGTGTTTTCGTGGCGCAgat                            <  2:351571/251‑1 (MQ=255)                                                                                                                                                                                                    gTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAgatg                           <  1:39629/251‑1 (MQ=255)                                                                                                                                                                                                        ggTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGGATGATTATTGAGGGGTTTCGTGgcgccggtggtgg                       >  2:87855/1‑242 (MQ=255)                                                                                                                                                                                                             gTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCATTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTTTTGAAGGTTTTCGTGGGGCCGATggtgaagatg                  >  1:405805/1‑251 (MQ=255)                                                                                                                                                                                                               cGATGGATGCATTTGCTGCATCAATCGGT‑CCACCTGCCCCCCTCCATAAACCGCAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGACACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGGATGGTTTTTTAGGGTTTTccgtgcgctggtgatgaggatga                 >  2:193633/1‑228 (MQ=255)                                                                                                                                                                                                                   ggatgCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGc            <  1:338999/251‑1 (MQ=255)                                                                                                                                                                                                                    gatgCATTTGCTGCATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGGTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGAtgaagatgaagaacc           <  2:175399/251‑4 (MQ=255)                                                                                                                                                                                                                             gctgcATCAATCGGT‑AAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCg  >  1:379635/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        CAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGT‑AAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCG  >  NC_000913/1905522‑1905992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑