Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,767,736	T→C	D317G (GAC→GGC)	ydiJ ←	putative FAD‑linked oxidoreductase YdiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,767,736	0	T	C	100.0%	140.7 / NA	43	D317G (GAC→GGC)	ydiJ	putative FAD‑linked oxidoreductase YdiJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/19);  total (24/19)

TTCAACCCCCGCCAGCTCACGGCACACCTGCCAGCCGATGACACCTGCTTGATGACTGGCGATCAGCTCATCCAACCGCGCACAGAGTGCATTTACCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTCGGTCATCTCATCGTTAAAGACATGACGCAGATCGTAACCGGTAAGAAAGCGG  >  NC_000913/1767498‑1767978                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   ttCAACCCCCGCCAGCTCACGGCACACCTGCCAGCCGATGACACCTGCTTGATGACTGGCGATCAGCTCATCCAACCGCGCACAGAGTGCATTTACCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTctct                                                                                                                                                                                                                                         >  1:97267/1‑250 (MQ=255)         ccGCCAGCTCACGGCACACCTGCCAGCCGATGACACCTGCTTGATGACTGGCGATCAGCTCATCCAACCGCGCACAGAGTGCATTTACCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAgc                                                                                                                                                                                                                                <  1:310858/251‑1 (MQ=255)             cAGCTCACGGCACACCTGCCATCCGATGACACCTGCTTGATGACTGGCGATCAGCTCATCCAACCGCGCACAGAGTGCATTTACCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGccc                                                                                                                                                                                                                            <  2:115252/251‑1 (MQ=255)             cAGCGCACGGCACACCGGCCAGCACATGACCACTGCTGGATGGCAGGCGATCAGCTCATCCAACCGCGCACAGAGGGGAGTTACACGCTCATCAATCCGCGCCTCATCAGCAGCAGCAAATCCCACAAGGTTCAGCGCGAGCATCGCTTGGTCAGGCACAGCGGTAAACAACGCGCTGACGGAATGCCAGACAAGAGCTTCCCGCGCCAGATTCAGCGCTTTGGAGCCCACCGTCTCGACCGAAAGCGccc                                                                                                                                                                                                                            <  2:190512/251‑1 (MQ=255)                     ggCACACCTGCCAGCCGATGACCCCTGCTTGCTGACTGGCGATCACCTCATCCAACCGCGCCCAGAGTGCCTTTACCAGCTCCTCCATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCCGCACTTTTGCGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTcc                                                                                                                                                                                                                    >  2:377054/1‑250 (MQ=255)                                   ccGATGACTCCTGCTTGATGACTGGCGATCAGCTCATCCAACCGCGCACAGAGTGCATTTACCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGTCTCAACCATAAACGgcgc                                                                                                                                                                                                      <  1:351056/251‑1 (MQ=255)                                   ccGATGACACCTGCTTGATGACTGGCGATCAGCTCATCCAACCGCGCACAGAGTGCATTTACCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGgcgc                                                                                                                                                                                                      >  2:134332/1‑251 (MQ=255)                                                   gatgaCTGGCGATCAGCTCATACAACCACGCAAAGAGTGCATTTAACCGATCCTCAATCAGCGCCTCATCATAACACGCAAATTACACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCGACTCGCTGACGGAATGACAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTAcgcagcgcag                                                                                                                                                                                        >  2:221340/1‑249 (MQ=255)                                                          ggCGATCAGCTCATCCAACCGCGCACAGAGTGCATTTACCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCaaa                                                                                                                                                                                 >  2:322950/1‑249 (MQ=255)                                                          ggCGATCAGCTCATCCAACCGCGCACAGAGTGCATTTACCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAag                                                                                                                                                                               <  1:170613/251‑1 (MQ=255)                                                                         cAACCGCGCACAGAGTGCATTTACCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCCCTTTTGAGCCCACCGTCTCTACCGCAAGCGCCCGCGCCTCAACCATAAACGGAGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGt                                                                                                                                                                >  2:393120/1‑251 (MQ=255)                                                                          aaCCGCGCACAGAGTGCATTTACCAGCTCATCAATCCGCGCCTCATCATCACCAGCAAATTCCACACTGTTCGGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGtt                                                                                                                                                               >  1:364687/1‑251 (MQ=255)                                                                              cggcACAGAGTGAATTTCCCCGCTCATAAATCAGCGCCTCATCAGCACCAGCAAATTCCACAATGGTCAGCCCGAGCATCTCTTGGTCAGGCACAGCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATCCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCATTGCGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCAcc                                                                                                                                                           <  2:336267/249‑1 (MQ=255)                                                                                           ctttACCCGCTAATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCCGCGCAGAGCCACAAGAGGCATATTTGACGTTCACCAGACGGCGCACtt                                                                                                                                              >  2:59616/2‑251 (MQ=255)                                                                                               aCCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAgg                                                                                                                                          >  1:105733/1‑251 (MQ=255)                                                                                                   gcacaccaatcaCCGCCTCATCATCACCAGCAAATTCCACAAATTTCAGCCCGAGCATCTCTTGGTCTGGCACACCGGTAAGCAACCCGCTGACGGAATGCCAGCCAATAGCTTCCCGCGCCAGATTCATCACTTTTGAGCCCTCCGTCTCTACCGAAAGCGCCCTCGCCTCATCCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACTTGCACCACACGGCGCACTTTATGCAAg                                                                                                                                      <  2:28713/245‑1 (MQ=255)                                                                                                      catcaATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCTCTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCg                                                                                                                                    <  1:390399/250‑1 (MQ=255)                                                                                                                       tcatcaCCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGcccagt                                                                                                                  >  2:136091/1‑247 (MQ=255)                                                                                                                            aCCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCgg                                                                                                             >  1:17664/1‑251 (MQ=255)                                                                                                                                                   gCCCGGGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCGGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCGCCAGACGGCGCACTTTAGGCATGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCt                                                                                      <  1:426513/251‑1 (MQ=255)                                                                                                                                                       gAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGCCAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAatat                                                                                                                            >  2:225523/1‑209 (MQ=255)                                                                                                                                                       gAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGGGCACTTTa                                                                                                                                            >  2:299547/1‑193 (MQ=255)                                                                                                                                                       gAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGGGCACTTTa                                                                                                                                            <  1:299547/193‑1 (MQ=255)                                                                                                                                                       gAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAatat                                                                                                                            <  1:225523/209‑1 (MQ=255)                                                                                                                                                           aTCTCTTGGTCAGGCACTTCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGAGTCCCTTCTGAACCCGt                                                                              >  1:353522/1‑251 (MQ=255)                                                                                                                                                            tctcTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCTTGACACTCTCTTCCCGTGCCAGATTCAGCCCTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGTGCCCCAACAATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGAGTGAAATATCCAGCAGCGCTTCGGTACTACAGGCCAGCGACCCTTCTGAACCCGTc                                                                             >  1:205488/1‑251 (MQ=255)                                                                                                                                                                 tGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAAt                                                                        >  1:371984/1‑251 (MQ=255)                                                                                                                                                                 tGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTGTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAg                                                                           <  1:408694/248‑1 (MQ=255)                                                                                                                                                                     cAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACCATATCTTCCCGAGCCCGATTCAGAACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCCTATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGCAATATCCAGCAGCGCGTCGGTAATAAAGGCCCGCGTCCCTTCTGACCCCGTCAgcttgcgc                                                                    >  2:38141/1‑244 (MQ=255)                                                                                                                                                                          acaTCGGTAATCAACTCGCTGACGGAATGCCAGACACTATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTc                                                                 >  1:277032/1‑249 (MQ=255)                                                                                                                                                                            agcGGTAATCAACCCGCTGACGGAATGCCAGACAATATACTCCCGCGCCAGATTCAGCACTTTGGAGCCCGCCGTCTCTACCGAAAGCGCCCGCGCCGCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCAGTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGt                                                             <  2:16373/249‑1 (MQ=255)                                                                                                                                                                            aTCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCTCCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCGGAACCCGTCAGAATGCGCGTCAGGt                                                             <  1:343260/251‑1 (MQ=255)                                                                                                                                                                                 tAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAAc                                                        >  2:299566/1‑251 (MQ=255)                                                                                                                                                                                   aTCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAATGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTc                                                      <  1:377054/251‑1 (MQ=255)                                                                                                                                                                                   aTCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTc                                                      >  1:93190/1‑251 (MQ=255)                                                                                                                                                                                                    aTGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTCGGTCATCTCATCGTTa                                      >  1:140222/1‑250 (MQ=255)                                                                                                                                                                                                       ccAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTCGGTCATCTCATCGTTAAAGa                                  <  2:97267/251‑1 (MQ=255)                                                                                                                                                                                                                    tCCCGCGCCAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTCGGTCATCTCATCGTTAAAGACATGACGCAGAt                      <  1:326275/250‑1 (MQ=255)                                                                                                                                                                                                                    ccccGCGCCAGATTCAGCCCTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTCGGTCATCTCATCGTTAAAGACATGACGCAGATc                     <  2:105733/250‑1 (MQ=255)                                                                                                                                                                                                                           ccAGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAACGCGACCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTCGGTCATCTCATCGTTAAAGACATGACGCAGATCGTAACCg              >  1:390295/1‑251 (MQ=255)                                                                                                                                                                                                                             aGATTCAGCACTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTAAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTCGGTCATCTCATCGTTAAAGACATGACGCAGATCGTAACCGGt            >  1:180432/1‑251 (MQ=255)                                                                                                                                                                                                                                       cTTTTGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTCGGTCATCTCATCGTTAAAGACATGACGCAGATCGTAACCGGTAAGACAGCgg  >  2:91369/1‑251 (MQ=255)                                                                                                                                                                                                                                         tgtGAGCCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTTACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTCGGTCATCTCATCGTTAAAGACATGACGCAGATCGTAACCGGTAAGAAAGCgg  <  2:340122/247‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   TTCAACCCCCGCCAGCTCACGGCACACCTGCCAGCCGATGACACCTGCTTGATGACTGGCGATCAGCTCATCCAACCGCGCACAGAGTGCATTTACCCGCTCATCAATCAGCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGGCGCGTTACGCAGCGCAGAGTCAAAAGAGTCATATTTGACGTTCACCAGACGGCGCACTTTAGGCAAGCGTGTAATATCCAGCCGCGCTTCGGTAATAAAGGCCAGCGTCCCTTCTGAACCCGTCAGAATGCGCGTCAGGTCGAACTCGGTCATCTCATCGTTAAAGACATGACGCAGATCGTAACCGGTAAGAAAGCGG  >  NC_000913/1767498‑1767978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑