Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,350,723	T→C	N106S (AAC→AGC)	fabI ←	enoyl‑[acyl‑carrier‑protein] reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,350,723	0	T	C	97.6%	139.3 / NA	42	N106S (AAC→AGC)	fabI	enoyl‑[acyl‑carrier‑protein] reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (19/22);  minor base A (1/0);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCACACCTTCCGGACCCATCGCGTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGC  >  NC_000913/1350491‑1350967                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     tcGCACACCTTCCGCACCCATCGCGTTCGCCATATAGCGCACGTTCGCTTCCAGAGAAGCTTTTGCCAGACCCATAACGTTGTAGTTCGCGATAGCGCGCTCATCGCCAAGGTAGCAAAGGGTCAGCAGGGAGGAACCCGGATTCAGCCTGGAGCGGCAAGCGTTTGCGATCGCAACGAAGCTGTAGGCGCTGATGTTGTGGGCAATTTCGAAGTCTTCACGGGTAACGGCGCTAACATAGTCACCactac                                                                                                                                                                                                                                    >  2:140637/2‑247 (MQ=255)            ccGGACCCATCGCGTTCGCCATATAGCGCCCGTTCGCTTCCAGCGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGCAGGAAAGGGTCAGCAGGGCAGACCCCGGATTCAGCATGGAGCGGCAAGCTGTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGATAACATAGTCACCATCCAGCTGTTCGcc                                                                                                                                                                                                                          >  2:264849/1‑250 (MQ=255)            ccGGACCCATCGCGTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGGCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTTCCAGTGCCACGAAGCTGTAGGAGCTGATGTCGTGGGCAAGTTGGAAGCCTTCACGGGTATCGGCGCGAACATAGTCACCAGCCGGCAGATCGCCa                                                                                                                                                                                                                         <  2:54945/251‑1 (MQ=255)                      cgcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAAc                                                                                                                                                                                                               <  1:428964/251‑1 (MQ=255)                       gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAAcc                                                                                                                                                                                                              >  2:368873/1‑251 (MQ=255)                        cgTTCGCCATTTAGCGCCCGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAAcc                                                                                                                                                                                                              >  1:130902/1‑250 (MQ=255)                         gTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCa                                                                                                                                                                                                             >  1:980/1‑250 (MQ=255)                         gTTCGACATATAGCGCACGTTCTCTTCCAGAGACGCTTTGGCCAGACCCATAACGGTGTAGATCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTGTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCa                                                                                                                                                                                                             <  2:980/250‑1 (MQ=255)                                             tCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTc                                                                                                                                                                                         <  1:325216/250‑1 (MQ=255)                                              cGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCATCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCaa                                                                                                                                                                                       <  1:410168/251‑1 (MQ=255)                                              cGCTTCCAGAGACGCTTTTGCCAGACCCAGAACGTTGGAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCGTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATGTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCaa                                                                                                                                                                                       <  2:130902/251‑1 (MQ=255)                                                                 gCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTcc                                                                                                                                                                     <  2:152651/250‑1 (MQ=255)                                                                            aaCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCg                                                                                                                                                          <  1:103893/250‑1 (MQ=255)                                                                              cGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAAc                                                                                                                                                       <  1:140067/251‑1 (MQ=255)                                                                                gtGTAGTGCGGGATAGCGCGCTCAGCTCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGAGCGCCAGGTGCAAAACCAATAGAGTGGACGAAGCCGGCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACAt                                                                                                                                                     <  1:210513/250‑1 (MQ=255)                                                                                     gTTCGGTATAGCGCGCTCAGCGCCAAGGTATGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGTGCAATTTGGAAGCCTTCACGGGTAACGGCGCTAACATAGGCACCATCCAGCTTATCGCCATGTGCAAATCCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGgtgt                                                                                                                                                <  1:419631/251‑1 (MQ=255)                                                                                                cgcgcTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCa                                                                                                                                     <  1:191872/251‑1 (MQ=255)                                                                                                 gcgcTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCTGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTTGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCAt                                                                                                                                    <  1:383619/251‑1 (MQ=255)                                                                                                            cAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAAc                                                                                                                          >  1:39296/1‑250 (MQ=255)                                                                                                             aaGGTAGGAAAGGGTCAGCAGGGGAGAACCCGGATTCAGCATGGAACGGCAAGCTTTTGCCATTGCACCGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAGACTTTCCGTAGTTCAGCGAACATGGTGTAGCTGCTGGCATCTTCTGCAACAt                                                                                                                        >  2:173880/1‑251 (MQ=255)                                                                                                             aaGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCACTTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAACACCAATAGAGTGTACGAAACCGTCAAATTTAGGCCAAACTTTCCCCAGTTCAGCGACAATGGTGTCGATGCTGGCAGCTTTTGCAACAt                                                                                                                        >  2:237345/1‑251 (MQ=255)                                                                                                                      aaGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGTGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCTCCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAGCCGTCAAATTTCGGCCAAACTTTCCCCAGTGCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCa                                                                                                               <  1:272229/251‑1 (MQ=255)                                                                                                                                agGGCAGAACCCGGATTCAGCCTGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTc                                                                                                     <  2:128988/251‑1 (MQ=255)                                                                                                                                    cAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGaa                                                                                                 <  2:288390/251‑1 (MQ=255)                                                                                                                                       aaCCCGGATTCCGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATCGTCACCATCCAGCTGATCGCCAGGCGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTACCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCACCATCGCACTGCCGAACGATGTCAGAAccc                                                                                              >  2:178737/1‑251 (MQ=255)                                                                                                                                            gggttCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAATCCGTCAAATTTCGGCCGAACTTTCCCCAGTTTAGCGAACATGGTGTCGTTGCTGGCGTCTTCTTGAACATCGCTCTGCAGAACGATGTCAGAACCCAATTg                                                                                         <  1:30247/248‑1 (MQ=255)                                                                                                                                                           cGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAAcc                                                                                                                                                                                            >  2:134897/1‑137 (MQ=255)                                                                                                                                                           cGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGGACGAAAcc                                                                                                                                                                                            <  1:134897/137‑1 (MQ=255)                                                                                                                                                                       gCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCttt                                                              >  1:96743/1‑251 (MQ=255)                                                                                                                                                                       gCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCtt                                                               <  2:332874/250‑1 (MQ=255)                                                                                                                                                                                    aGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTc                                                 >  2:16433/1‑251 (MQ=255)                                                                                                                                                                                            gAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGt                                         >  1:336044/1‑251 (MQ=255)                                                                                                                                                                                            gAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGt                                         <  2:290214/251‑1 (MQ=255)                                                                                                                                                                                            gAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGCGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGt                                         >  1:290214/1‑251 (MQ=255)                                                                                                                                                                                            gAGCTGAGGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTGCAGGTTGTCGGTCTGGTAGGt                                         <  2:385581/251‑1 (MQ=255)                                                                                                                                                                                                         gggCAATTTTGAAGCCTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTc                             >  2:145437/1‑250 (MQ=255)                                                                                                                                                                                                            cAATTTTGAAGCCTTCCCGGGTAACGGAGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCTAATTCTTCTACGCGGCCTTTCAGGTTGTCGTTCTGGTAGGTGACTGCCAGTTCAGCt                         >  2:80674/1‑251 (MQ=255)                                                                                                                                                                                                                       ccTTCACGGGTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGCATGCCAGTTctactattcaggcaat              >  2:340887/1‑236 (MQ=255)                                                                                                                                                                                                                              gggTAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGcc       >  1:387023/1‑251 (MQ=255)                                                                                                                                                                                                                                 tAACGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGATACCGTCAACTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTg     >  1:35794/1‑250 (MQ=255)                                                                                                                                                                                                                                  aaCGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGATCAGCTCCTTCGCGGCGCATCGCCTg     >  1:168237/1‑249 (MQ=255)                                                                                                                                                                                                                                   aCGGCGCTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGc  <  1:23035/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     ACGCACACCTTCCGGACCCATCGCGTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGAGCGGCAAGCTTTTGCCATTGCAACGAAGCTGTAGGAGCTGATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGC  >  NC_000913/1350491‑1350967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑