Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	709,364	A→G	D344G (GAT→GGT)	chiP →	chitooligosaccharide outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	709,364	0	A	G	100.0%	161.4 / NA	54	D344G (GAT→GGT)	chiP	chitooligosaccharide outer membrane channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/31);  total (23/31)

GCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCC  >  NC_000913/709121‑709599                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            gCCCGTTAACCACCAGCTACCAGATCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGt                                                                                                                                                                                                                                      >  2:182630/1‑251 (MQ=255)  cccGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCGACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGtt                                                                                                                                                                                                                                     <  2:384088/251‑1 (MQ=255)        aaCCCCCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAGGGCACCTGTGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACt                                                                                                                                                                                                                               <  2:313298/251‑1 (MQ=255)         accaccAGCGACCAGGTCGACGTTACCCGAGATAAAGTTGACGATCGCAGCTTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCGTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGCCGCCTGGATATCTGGTGGGGTAACCGTTCTGACtt                                                                                                                                                                                                                              <  2:154239/251‑1 (MQ=255)                   aCCAGTTCTACGGTCCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTAAGCCGGCACCGCCTGGCGGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCaa                                                                                                                                                                                                                     <  2:1169/250‑1 (MQ=255)                   aCCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGTCTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCa                                                                                                                                                                                                                      <  1:74996/249‑1 (MQ=255)                      aGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACggg                                                                                                                                                                                                                 >  1:40855/1‑250 (MQ=255)                              ggTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGc                                                                                                                                                                                                         >  1:3115/1‑251 (MQ=255)                              ggTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACATTTATGCCGGCACCGACTGGCTGCAAGAGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGc                                                                                                                                                                                                         >  2:377071/1‑251 (MQ=255)                                 aCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGt                                                                                                                                                                                                      <  1:237759/251‑1 (MQ=255)                                           aaGTTGACGAGCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGt                                                                                                                                                                                            <  2:285002/251‑1 (MQ=255)                                                    aTCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAATGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTAt                                                                                                                                                                                   <  1:219117/251‑1 (MQ=255)                                                            gTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGaa                                                                                                                                                                           <  2:209064/251‑1 (MQ=255)                                                               caCGACCTTAATGACGGCGCCGCCCGTCTGCGAGCGTTGACCGTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGTCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGaaaaa                                                                                                                                                                        <  2:328776/250‑1 (MQ=255)                                                                  gACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTg                                                                                                                                                                     <  1:13299/251‑1 (MQ=255)                                                                   aCCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTgg                                                                                                                                                                    <  1:103483/251‑1 (MQ=255)                                                                         aTGACCGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTAACGGGCGGCTGACGAAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGTTGTATGACCTGAAAAACTGGAATCtt                                                                                                                                                              >  2:359245/1‑251 (MQ=255)                                                                                  ccGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTc                                                                                                                                                     <  1:310588/251‑1 (MQ=255)                                                                                        ggCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACTGTCAGCAGTGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGGCAACGGCGAAAAAGCGGTCTTCTTCGTTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCAt                                                                                                                                                <  1:197603/250‑1 (MQ=255)                                                                                         gCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCg                                                                                                                                              <  1:5082/251‑1 (MQ=255)                                                                                                     gACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTCTGACCTGAAAAACTGGACTCTTCCAGGCTTCGCCATCGGCGCTTCCTACg                                                                                                                                  >  2:40990/1‑251 (MQ=255)                                                                                                          ttggtgagcGGGCGGCTGACGGAGTGGAGTTGCGCCTCGAAGGCACCTGGGTTGAGGCTGACGGTAATCAGGGATACGTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACTGTTCTGACTTCAATGCCAACGGCGAAAAAGCGGTCTGCTTCGGTGCGAGGTATGACCTGAAAAACTTGAATCTTCCAGGCGTCGCCATCGGCGCTTCCTACGttt                                                                                                                               <  2:92872/241‑1 (MQ=255)                                                                                                                aCCGGGCGGCTGACGTAGTGGCTTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATgg                                                                                                                       >  2:65274/1‑251 (MQ=255)                                                                                                                 ccGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGCCGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCACACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCCACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATggg                                                                                                                      >  2:167370/1‑251 (MQ=255)                                                                                                                  cGGGCGGCTCACGTAGTGGATTTGCGCATCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTAAAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATggg                                                                                                                      >  1:209137/1‑250 (MQ=255)                                                                                                                   gggCGGCTGACTTAGTGGATTGGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGTGTATCCGTTCTGACTTCAACGCCAACGTCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGTAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGAt                                                                                                                    <  1:41467/251‑1 (MQ=255)                                                                                                                     gcggcTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGc                                                                                                                  <  1:40990/251‑1 (MQ=255)                                                                                                                            aCGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATggg                                                                                                                      >  1:107590/1‑240 (MQ=255)                                                                                                                            aCGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATggg                                                                                                                      <  2:107590/240‑1 (MQ=255)                                                                                                                            aCGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACGTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACTTTTACGCATGGGATGCTAAACCt                                                                                                           <  1:364425/251‑1 (MQ=255)                                                                                                                                       gtGCGCCCCGAAGGCACCTGGGGTAAGGCTGACGGTCAGCAGGGATACTTCCTGCATCGTATGACGCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTGCTGACTTCAACGCCAACGGCGAAAAAGCGGTCGTCGTCGGTGCGATGTATGACCTGAAAATCTGGAATCGTCCAGGCTTCGCCATCGGCGCTTCCTACGTTGACGCATGGGATGCTAACCCTGCGACCTGGc                                                                                                 <  2:41719/249‑1 (MQ=255)                                                                                                                                        ttcgcCTCGACGGCACCTGGGTGAAGGATGACGGTCAGCAGGGATACTTCCTGTAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGACAACGGCGAAAAAGAGGACTTCTTCGGCGCGATGTATGCCCTGACAACCTGGAATCTTCCAGGCTTCGCAATCGGCGCTTCCTACGTTTACGCATGGGCTGCTAAAccaccgaactggc                                                                                                 >  2:342265/3‑238 (MQ=255)                                                                                                                                               cGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATc                                                                                        <  1:153953/251‑1 (MQ=255)                                                                                                                                               cGAAGGCACCTGGGTTAAAGCTGACGGTCAGCAGGGATACTTCCTGCAGCGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGCTATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATc                                                                                        >  2:138796/1‑251 (MQ=255)                                                                                                                                                    gCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGAt                                                                                   <  1:297702/251‑1 (MQ=255)                                                                                                                                                     ccacgtggttaatccTGACGGTCAGCAAGGATCCTGCCTGCATCGTATGACTCCAACCTACGCTTCCTCAAATGGTCGCCTGGATATCTGGTGGGGTAACCGATCTGACTTCAACGCCAACGGCGAAAAACAGATCTTCTTCGGTGCGACGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCCTACGACGTTTACGCATGGGATGCTAAACCTGCGACCTGCCAGAGCAAACCGGAtc                                                                                  <  2:5267/237‑2 (MQ=255)                                                                                                                                                      cccTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCATCCTACGCTTCCTCAAACGGTCGCCTGGACATCTGGTGGGGTAACCGTTCTGACATCAACGCCAACGTCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTGCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACTCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCTGATGc                                                                                 <  1:342265/250‑1 (MQ=255)                                                                                                                                                      aCCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGaa                                                                                                                                                                           >  1:235867/1‑161 (MQ=255)                                                                                                                                                           ggTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGtact                                                                            <  2:318718/251‑1 (MQ=255)                                                                                                                                                                     gACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCTTACTACGACaaaaa                                                                  <  2:67761/251‑1 (MQ=255)                                                                                                                                                                       cGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAAcc                                                                >  2:327079/1‑251 (MQ=255)                                                                                                                                                                           cagcagGGCCACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCACACGGTCGCCTGGATATCTGGTGCGGTCACCGTTCTGACTTCAACGCCCACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCCATCCGGATGCGTACTACGACAAAAACCggcc                                                            >  2:147211/1‑249 (MQ=255)                                                                                                                                                                            agcagGGATACTTCCTGCAACGTATGACTCCAACCTACGCTCCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAGCGGCGAAACAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGCTGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACt                                                           >  1:208763/1‑251 (MQ=255)                                                                                                                                                                               agGGATACTTCCTGCAACGTATGACTCCAACCTACGCCTCCTCAAACGGTCGCATGGATATCTCGTGGGGTCCCCGATCTGACTTCAACGCCACCGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGACTCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCAGGATGCGTACTACGACAAAAACCGGCCTAtt                                                        >  1:424626/1‑251 (MQ=255)                                                                                                                                                                                             cAACGTATGACTCCAACCTACGCGTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTGCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGGCTGCCTa                                          <  2:150815/251‑1 (MQ=255)                                                                                                                                                                                                        cccAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATg                               <  2:200736/250‑1 (MQ=255)                                                                                                                                                                                                                     tCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATAcc                    <  2:165397/249‑1 (MQ=255)                                                                                                                                                                                                                      ccTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCAtt                 <  1:303029/251‑1 (MQ=255)                                                                                                                                                                                                                       cTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCCATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTc                >  2:71849/1‑251 (MQ=255)                                                                                                                                                                                                                            aCGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGCTGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGAc           >  2:333772/1‑251 (MQ=255)                                                                                                                                                                                                                               gTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAACAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGt        >  2:50015/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTcgcg    >  1:298527/1‑251 (MQ=255)                                                                                                                                                                                                                                     tGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTCTGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTCCGTTTACGCATGGGATGCTACACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACCGCCTGGATGCGGACTATACCATTCAGGACGGTCGCGcc  >  2:221837/1‑251 (MQ=255)                                                                                                                                                                                                                                     tGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGTATGCGGTCTATAcccatcaggcaggacgcgcc  >  2:185416/1‑233 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            GCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCC  >  NC_000913/709121‑709599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑