| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,727,494 | (G)6→7 | coding (836/1098 nt) | nemA → | N‑ethylmaleimide reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,727,494 | 1 | . | G | 85.7% | 76.6 / 6.9 | 28 | coding (836/1098 nt) | nemA | N‑ethylmaleimide reductase |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (7/17); total (9/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
AACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGG‑TG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGAT > NC_000913/1727250‑1727741 | aaCCATCGTACCGATCAGTACGGCGGCAGCGTGGCAAATCGCGCACGTTTGGTCCTGGCAGCGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCCCCAATCGGTACTTTCCAGAACACAAATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAAACAGATTGGG‑CG‑G‑GGGGGTG‑AAc > 2:202140/1‑251 (MQ=255)aaCCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGGCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATACACTGTATCTGATTGAACCACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AAc > 1:226002/1‑251 (MQ=255) tCAGTACGGCGGCAGCGTGGAAAATCGCGCCCGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGCTCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGG‑GGGAACCGTATACTGATGc > 2:280774/1‑250 (MQ=255) tCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGc < 1:280774/250‑1 (MQ=255) gTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTc < 1:35456/251‑1 (MQ=255) ggAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTAcg < 1:83286/250‑1 (MQ=255) aTCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATGGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTAGCGGCATATGTCAGAACCAGATTGGG‑CG‑GTGGGG‑TG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCg < 1:66633/251‑1 (MQ=255) ggTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTGCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTAGCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTTCATATGTCAGAACCAGATGGGG‑CG‑G‑GGGGGTT‑AATTGTATACTGATGGGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCg < 1:409274/251‑1 (MQ=255) aCTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATGGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGAtt < 1:368535/251‑1 (MQ=255) aCTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGAtt < 1:100724/251‑1 (MQ=255) ccGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa < 1:214368/251‑1 (MQ=255) ggATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTAGCTGCATATGTCAGAACCAGATTGGT‑CG‑G‑GGGGGTG‑ATCCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCTCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac < 1:177871/251‑1 (MQ=255) gaagaaTGGGGAGCCGATCGCATTGGACTTCCCGTCTCAGCAATCGGTACTTTCCAGAAACCAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAAACACTGGGCAAACGCGGCATTGCTTATCTGCATATGTCAGAAACCGATTGGGAGG‑G‑GGGG‑TG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGAGCCCGTTTCCACGGACCGATTATCGGCGCAGGGGCATACACAGt > 2:278087/1‑249 (MQ=255) aTCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGCTTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACACTAGAAAAAGCTGAAACGCt > 2:334301/1‑251 (MQ=255) ggCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGc < 2:371347/250‑1 (MQ=255) gCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTAGCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCaa < 1:431638/251‑1 (MQ=255) ttCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCTATGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACCCAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAGTTGATGCGGTGGCATTTg > 2:389619/1‑251 (MQ=255) ccAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAGCTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCGGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTTGCATTTgg < 1:215216/250‑1 (MQ=255) ccAGAACACAGATAACGGCCCGAATGAAGAAGCCGATCCTCTTTATCTCCTTGAACAACTGGGTAAACGCCGCATTGCTTATCTGCATATGTCCGAACCCGATTGGG‑CG‑G‑GGGGGCC‑CACCGTATACTGCTGCGTTCCGCGACAACCTACGCGCCCCTCTCCCCGGTCCGCCCCCCGGCGCAGGTGCATACACAGTAGCATCAGCTGAAACGCTGCTCTGCAAAGGGCTAATTGACGCGCTGGCacctgct > 1:85796/1‑245 (MQ=255) ggCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCTGTGGCATATGGTCGTGACTGGATTGCGa < 1:105968/251‑1 (MQ=255) ggCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCg > 1:159203/1‑250 (MQ=255) cACTTTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGT‑CGTG‑GGGG‑TT‑AACCGTATACCGATTCGTTCCGCGAAAAAGGACGCGCCCGTTTCCACGGTCCTATTATCGGCGCAGGTGCATACACATTAGAAAAAGCTGAAACGCTGATCGGCAAAGTGTTAATTGATGCGTTGGCATTTGTTCGTGACTGTATTGCGAACCCGGATCTGGTCGCCCGCtt < 1:374962/251‑1 (MQ=255) acaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCAGTTTCCGCGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGCCGTGACTCTATGGCGAACCCGGATCTGTTCGCCCGGTTGCAGCGCAAAGCTGAg < 1:134455/251‑1 (MQ=255) acTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTGCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCGGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGTGACTGGAGTGCTAACCCGGATCTGGTCGCCCGCGTGCATCGCAAAGCTGAGCtt < 2:159203/251‑1 (MQ=255) cgggaTTGCTTAACTGCAAATGGCAGAAACAAGTTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGt < 1:202642/247‑1 (MQ=255) aTCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGttt > 1:130128/1‑251 (MQ=255) atatGTCAGAACCAGATGGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTATAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGTTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCGTTCAGCGCAAAGCTGAGCTTAACCCACAGCGGGCCGAAAGTTTCTACgg < 2:61681/251‑1 (MQ=255) gATTGGG‑CG‑G‑GGGGGTG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAg < 2:82952/251‑1 (MQ=255) gggg‑TG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTGTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGAt < 1:244302/251‑1 (MQ=255) | AACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGG‑CG‑G‑GGGG‑TG‑AACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGAT > NC_000913/1727250‑1727741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |