Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,593,308	Δ1 bp	coding (130/714 nt)	livF ←	branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,593,308	0	T	.	95.3%	340.1 / 7.6	86	coding (130/714 nt)	livF	branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base . (38/43);  total (38/48)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTTTCCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATC  >  NC_000913/3593065‑3593556                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         gaaccAGCTAATCCACCCACGTTAGGCCCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ACCGCCAGGTCCTCTTCCACCGTCATCCGCGAGAATACGCTACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCTTCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCTAGGAAGTTGGTTT‑ccccgccc                                                                                                                                                                                                                                                  <  2:165120/247‑1 (MQ=255) gAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑CCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑ccccgccc                                                                                                                                                                                                                                                  >  2:84112/1‑251 (MQ=255)  aaaCAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑ccccgcccc                                                                                                                                                                                                                                                 >  2:153855/1‑251 (MQ=255)    aCAGCTCATCCCCCCACTTTAGGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCATGTTCTCTCCCCCCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGt                                                                                                                                                                                                                                               <  2:175845/251‑1 (MQ=255)      aGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTACTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TCTCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTc                                                                                                                                                                                                                                             >  1:386954/1‑251 (MQ=255)      aGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTAAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTCCCACCGTCATCCGCGAGAAGACGCGCCGCCCTTCCGGGACAATCGCCCCCGCTTCGCGCA‑TGATTTTC‑GCTGTCGGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTc                                                                                                                                                                                                                                             <  1:239920/251‑1 (MQ=255)          cATACCCCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGTTTCTCTTCCACCGTCATCCGCGAAAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGCCGGTAATG‑TCTT‑TATGACCAAACACATTTCGCCCGCTGGTGGCACGCGGATCGCCGCGTAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCgcgc                                                                                                                                                                                                                                         <  1:270302/251‑1 (MQ=255)           aTACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGcc                                                                                                                                                                                                                                        >  1:120776/1‑251 (MQ=255)             acacCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCCGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGGGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCa                                                                                                                                                                                                                                       >  2:218651/1‑250 (MQ=255)              cccccccTTTATGCGCCCCTGGAACTGGTCGCGTTCACCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAACCACAATTCGCCCGCTGGTGGCACGCGGAGCGCCGCATAACGTGCCGAGAAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAAt                                                                                                                                                                                                                                     <  1:121622/245‑1 (MQ=255)               ccccACTTTATGCGCTCCTGGAACTGGTCGCGTTCAACAAAAAACCCGCCC‑ACCGCCAGGTCCCCTTCCACCGTCATCCGCGAGAAGCCGCGACGCCCTTCCGGGACACTCGCCACCGCTCCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTG‑TATCATCAAACCCAATTCGCCCGCTGGTGGCACGCGGATCCCCGCTTACCGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATc                                                                                                                                                                                                                                    <  1:289684/250‑1 (MQ=255)               acCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATc                                                                                                                                                                                                                                    >  2:75102/1‑251 (MQ=255)                      ttgtGCGCTCCGGGAACGGGTCGCGTTCAGCAAAAAAACCGCCC‑ACCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCGGTCGGTAATG‑CCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGt                                                                                                                                                                                                                               <  1:67432/246‑1 (MQ=255)                      ttATGCGCTCCGGGATCTGTGCGCGTCAAACAAAAAAACCGCCC‑CTCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGCCGCCCTTCGGGGACAATCGCCACGGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGACCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTg                                                                                                                                                                                                                              <  2:307358/250‑1 (MQ=255)                          gcgcTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGACGACGCGACGCCATTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGCTCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAAt                                                                                                                                                                                                                         >  1:270531/1‑251 (MQ=255)                          gcgcTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAAt                                                                                                                                                                                                                         >  1:214140/1‑251 (MQ=255)                           cgcTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCAGCGCGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAAtc                                                                                                                                                                                                                        >  1:70239/1‑251 (MQ=255)                            ccTCCTGGAACTGGTCGCGTTCAGCAAAAAACCCGCCC‑ACCGCCAGGTTCTCTCCCCCCGTCATCCGGGAGAAGCCGCGACGCCCTTCCGGGACAATCGCCCCCGCTTCGCGCA‑TGATTTGC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TACCAGCAAACACAATTCGCCCGCGGGTGGCACGCGTATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAAtct                                                                                                                                                                                                                       <  1:63780/250‑1 (MQ=255)                                cTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGCGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGcc                                                                                                                                                                                                                   >  2:204545/1‑251 (MQ=255)                                  ggAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGccc                                                                                                                                                                                                                  <  1:303133/250‑1 (MQ=255)                                   gAACTGGTCGCTTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCCTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCTCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACCCAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCtg                                                                                                                                                                                                                <  1:119328/251‑1 (MQ=255)                                      attggtgccgttaggcaaaaaaCCGCCCCACCGCCAGGTTCTCATCCTCCGCCATCCGCGAGAAGACGTGACGCCTTTCCGGGACAATCGCCACCCTTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑GATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAG‑TGGGTTTCCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCtgat                                                                                                                                                                                                              <  2:242725/235‑1 (MQ=255)                                        ggTCGCGTTCAGCAAAAAACCCGCCC‑ACCGCCAGGTTCTCTTCCCCCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGG‑GGGTTTCCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCtgatt                                                                                                                                                                                                             <  1:234367/249‑1 (MQ=255)                                             cgtcaggaaaaaaaaCCGCCC‑ACCGCGCGGTTCTCTGCCACCCTCACCCGCAAAAAGACGCGACGCCCTTCCGGGCCAATCGCCACCGCTTCGCGCA‑TGATTTCC‑GCGTTCTGCAAGTCGGTAATG‑TTTT‑TAGCATCAAACACAATCCGCCCGCGGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAGGGTGGTTG‑CCCCGCCCCGTTGGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGc                                                                                                                                                                                                      <  2:41590/243‑1 (MQ=255)                                                tCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAg                                                                                                                                                                                                    <  1:272283/250‑1 (MQ=255)                                                     aaaaaacCCGCCC‑ATCGCTAGGTTCTCTCCCTCCGTCATCCGCGCGAAGCCGCGACGCCCTTCCTGGACAATCGCCACCCCTCCGCGCA‑TGATTTTC‑GCTTGCTGCCAGTCTCTAATG‑TCTT‑TATCATCAAACACAATTCGCCCTCTGTTGCCACTCGGATCGCCGCATAACGTGCCGAGCAAGGTG‑TTTTCCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGGATGCTCAc                                                                                                                                                                                              <  1:97342/251‑1 (MQ=255)                                                        agaacccccgcaccGCCAGTTTCTCTCCCGCCGGCAGCCGCGAGAGGCCGCGCCGCCCTCCCGGAACAATCGCCCCTGTTCCGGGCA‑GAATTTGCTGGTGT‑TGCCGGTCGGTAATG‑TGTT‑TTTCATCAACCCCAATTCGCCCGCTGGTGGCAGGCGGTTCGCCGGATAACGGGCCGGGCAAGG‑GGTTTTCCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCTCGGATGGATATGCAGGcgcgcct                                                                                                                                                                                            <  2:386954/238‑7 (MQ=255)                                                        aaaaCCGCCC‑ATCGCCAGGTGCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGAA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTc                                                                                                                                                                                           <  2:120776/251‑1 (MQ=255)                                                         aaaCCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGACGACCCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTc                                                                                                                                                                                           >  1:35389/1‑250 (MQ=255)                                                          acccGCCC‑CTCGCCAGGTTCTCTCCCACCGTCACCCGCGAGAAGACGCGACGCCCTCCGGGGACAAGCGCCCCCGCTTCGCGCA‑GGATTTTC‑GCGGGCTGCCAGGCGGTAATG‑TTTG‑TATCATCAAACACAATGCGCCCGTTGGGGGCACGCGGATCGCCGCATAGCGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCAt                                                                                                                                                                                         <  2:70239/249‑1 (MQ=255)                                                                      gCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTgg                                                                                                                                                                              >  1:211966/1‑251 (MQ=255)                                                                         aGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTTcccc                                                                                                                                                                                                                                                       <  2:305911/176‑1 (MQ=255)                                                                         aGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTTcccc                                                                                                                                                                                                                                                       >  1:305911/1‑176 (MQ=255)                                                                         aGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGAtt                                                                                                                                                                           <  1:27834/251‑1 (MQ=255)                                                                          ggTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGAttt                                                                                                                                                                          >  2:333429/1‑251 (MQ=255)                                                                           gtcctctTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGAtttt                                                                                                                                                                         <  1:75914/248‑1 (MQ=255)                                                                              ctctTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGcc                                                                                                                                                                      >  1:415021/1‑251 (MQ=255)                                                                               tctTCCACCGTCATCCGAGAGAAGACGCGACGCCCTTCTGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTA‑GCAGTCTGCAAGTTTGTAAAG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACACGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCCGGCTCACCTCCTGCACCGCCTGGATTTTGCCg                                                                                                                                                                     >  1:182212/1‑251 (MQ=255)                                                                                 cgcccccgcccgtcgcgAGAAGACCCGACCCCCTTCCGGGAAACTCGCCACCGCTT‑GCCCAATGATTTTC‑GCTTGCGTCCAGTCGGTAATC‑TCTT‑TATCATCAAACACAATCCGCCCGCGGGTGGCACGCGGATCGCCGCATAACGGGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCGCCTCATGCAGCGCCTGGATTTTGCCGTa                                                                                                                                                                   <  2:189248/238‑1 (MQ=255)                                                                                  tcccccGTCATCCGCGAGAAGACGCGACGCCCTTCCGGTACAAGCGCCACCGCTACGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TAGCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCGGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCg                                                                                                                                                                     <  1:416188/244‑1 (MQ=255)                                                                                              cgcgAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑CCTT‑TATCATCAAACACAATCCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACt                                                                                                                                                      <  1:84112/251‑1 (MQ=255)                                                                                               gcgAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACtt                                                                                                                                                     >  2:64953/1‑251 (MQ=255)                                                                                                 gagaAGACAAGACGCCCTTCCGGGACAATCGCCACCGCTTCACGCC‑TGCCTTTC‑GCTGTATGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGAGGCTCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGACCTGATTGATATGAAGGCTCACCTCCTGCAGCGCCTGGATTTTGCCGTAGTGGCCGCTGACtttt                                                                                                                                                   >  2:356571/1‑250 (MQ=255)                                                                                                  agaagaCGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTATGCCGTAGTGGGCGCTGCCTTTGt                                                                                                                                                  >  2:17240/1‑251 (MQ=255)                                                                                                  agaagaCGCGACGCACTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCCGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGGGGGCGCTGACTTTGt                                                                                                                                                  >  2:49051/1‑251 (MQ=255)                                                                                                   aaagaCGTGTCGCCCTCCCGGGACACTCGCGCCCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGGCGGTAATT‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGGGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTGTGTc                                                                                                                                                 <  2:389320/250‑1 (MQ=255)                                                                                                      gacgcgacccCCTTCCGGAACAACCGCCACCTCTTCGCGCA‑TGATTTTC‑GCTGCCTGCCAGTCGGTAATT‑TCTT‑TATCATCAAACCCAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCaaa                                                                                                                                              <  2:415021/251‑1 (MQ=255)                                                                                                        cgcgacgcCCTTCCGGGACAACCGCCCCCGCTTCGCGCA‑TGATTTTC‑GCTTTCTGCCATTCGGTAAGG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTTGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTGGCCGTAGTGGGCTCTGACTTTGTCAAAgg                                                                                                                                            <  1:142579/251‑1 (MQ=255)                                                                                                              gcCCTCCCGGGACAATAGCCACCGCTTCGCGCA‑TCATTTTC‑GCTGTCTGCCAGCCGGTAATC‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATAGCCGCATAACGTGCCGAGCACGGTGGTTT‑CCCCGCCCCGATCGCGCCAATCAGCGTGACACTCCCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGCTTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGacaac                                                                                                                                       >  2:401573/1‑250 (MQ=255)                                                                                                                ccTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATg                                                                                                                                    <  1:153855/251‑1 (MQ=255)                                                                                                                ccTTCCGAGACACTAGCCCCCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGCAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGCGGTTT‑CCCCGCCCCGTTCGCGCCAATCCGCGTGACAATATCGCCCTGCTTGCTATGAAGGCTCACCTCATGCCGCGCCTGGATTTTGCCGTCGTGGGCGCTGACTTTGTCAACGGatatcatg                                                                                                                                    >  2:44020/1‑244 (MQ=255)                                                                                                                       ggACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACttttt                                                                                                                             <  1:80821/251‑1 (MQ=255)                                                                                                                            aTCGCCACCGCTTCGCGCA‑GGATTTTC‑GCTGCCGGCCGGTCGGTAATG‑TCTT‑GAGCATAAAACACAATCCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTGTTCCATc                                                                                                                        <  2:55627/251‑1 (MQ=255)                                                                                                                                         cgcgCC‑TGTTTTTC‑TCTGTCGGCCATACTGTAATG‑TCTTGTA‑CATCAAACACATTTCGCCCGTTGGTGGCCCGCGGTCCGCCGCATAACGGGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCAGGCAGCGCCTGGATTTTGCCTTTGTGGTCGCTGACTTTGTCAAAGGACAACATGTCTTTTGCCATcggattcttcacct                                                                                                           <  2:272923/251‑14 (MQ=255)                                                                                                                                          gcgcA‑TGATTTTC‑GGTGTTTTCCAGTCGGTA‑TGCGATA‑TATCATCAACCACAATTCGCCCGTTGGTGGCTCGCGGATCGCCGGATAACGTGCCAAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCACATGCAGCGCCTGGATTTTGCCGTAGTGGTCGCTGACTTTGTCAAAGGACAACATGACGTTTTCCTTCGTATGCCTCAcata                                                                                                          <  1:356810/251‑4 (MQ=255)                                                                                                                                             cA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCGGGGGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTGTTCCAGCGTATGCCTCACCTAAAt                                                                                                       <  1:110730/251‑1 (MQ=255)                                                                                                                                                         gCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGTATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCACAGCCTGGATTTTGCCGTACTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAgtcccggct                                                                                             >  2:84740/1‑243 (MQ=255)                                                                                                                                                              cTGCCAGTCGGTAATG‑TCTT‑TATCCTCAAACACCATTCGCAAGATGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGCGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGCTATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGAATTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGt                                                                                        >  2:417100/1‑251 (MQ=255)                                                                                                                                                              cTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGt                                                                                        >  1:262067/1‑251 (MQ=255)                                                                                                                                                                  cAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCgg                                                                                    >  1:222372/1‑251 (MQ=255)                                                                                                                                                                    ggcGGTAATG‑TCTT‑TAGCAACAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCAAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGt                                                                                  <  2:80990/249‑1 (MQ=255)                                                                                                                                                                    gTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTAACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGt                                                                                  >  1:92940/1‑251 (MQ=255)                                                                                                                                                                    gTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCACGCCACGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATCCCTCACCTAAATAGGCACGGATCACGTCCGGGt                                                                                  >  1:294549/1‑251 (MQ=255)                                                                                                                                                                           aTG‑TCTT‑TATCATCAAACACAATTCGCCCGCGGGTGGCACGCGGATCGCCGCATAACGTGCCGATCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAATGACAACATGACTGTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACg                                                                           <  1:384052/251‑1 (MQ=255)                                                                                                                                                                             g‑TCTT‑TATCACCAAACACAATCCGCCCGCTGGTGGCACGCGGCTCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACgg                                                                          <  2:3340/250‑1 (MQ=255)                                                                                                                                                                                  t‑tATCATCAAACCCAATGCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGAt                                                                        <  1:75102/248‑1 (MQ=255)                                                                                                                                                                                    tATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGc                                                                    >  1:132487/1‑251 (MQ=255)                                                                                                                                                                                       catcaAACACAATTCGCCCGCTGGTGGAACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTcc                                                                 >  1:164698/1‑251 (MQ=255)                                                                                                                                                                                         tcaAACACAATTCGCCCGCTGGTGGCACGCGGATCCCCGCATACCGTGCCGCGCAAGGTGGTTT‑ACCCGCCCCCTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCCCCgg                                                               >  2:27875/1‑251 (MQ=255)                                                                                                                                                                                          caAACACAATCCGCCCGCTGGGTGCACGCGCTTCGCCGCATAACGTGCCGGGAAAGGTGGTGT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCAGCTCATGCAGCGCCGGGATATTGTCGTAGTGTGCGCTGACTTTTTCAAAGGACAACATTAGTTTTTCCTTCTTATTCCGTACCTGAATAGACACGGATCACGTCGGGAGTATCACCGATCTGCTCCgg                                                               <  2:249685/250‑1 (MQ=255)                                                                                                                                                                                            aaCACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGt                                                            >  1:140575/1‑251 (MQ=255)                                                                                                                                                                                                 aaTTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCCAAGGACAACATGACTTTTTCCATCTTCTGCCTCACCTAAATAGGCACGGTTCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGt                                                       >  2:138654/1‑251 (MQ=255)                                                                                                                                                                                                 aaTTCGCCCGCTGGGGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGt                                                       <  2:211966/251‑1 (MQ=255)                                                                                                                                                                                                      gCCCGCTGGTGGCAAGCGCATCGCCGCACAACGTGCCGAGAACGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGAGTGACAATCTAGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGAAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTGTGcc                                                  >  2:49785/1‑251 (MQ=255)                                                                                                                                                                                                          gCTGGTGGCACGCGGATCGCCGCATCAAGTGCAGAGCAAGGCGGTTT‑CCACGCCCCGTTCGCGCCAATCAGCGGGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGACCAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCGCCGGCGAACCGTTTGCCAgcg                                              >  2:254629/1‑251 (MQ=255)                                                                                                                                                                                                               tggCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGCTATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTc                                         >  2:80543/1‑251 (MQ=255)                                                                                                                                                                                                               ggacACGCGGATCGCCCCCTAGCGGACCAAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGTCTCTCCTCATGCGGCGCCTGGATTATGCCGTAGTGGGCGCGGACTTTGTCAAAGGACAACATGCCGTTCTCCACCTTATGCCTCACCTAAATTGGCACGGATCACGTCTTGGTTAAGCCGGATCTTCTCCGGCGTACCGTTTGCCAGCGGCGTc                                         <  1:395204/248‑1 (MQ=255)                                                                                                                                                                                                                   ccgcgGAGCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCt                                     <  2:214140/250‑1 (MQ=255)                                                                                                                                                                                                                       ggATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGAtt                                 <  1:98535/251‑1 (MQ=255)                                                                                                                                                                                                                       ggATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGAtt                                 >  1:65033/1‑251 (MQ=255)                                                                                                                                                                                                                       ggATCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGAtt                                 <  1:333429/251‑1 (MQ=255)                                                                                                                                                                                                                         accgccgcATAACGTGCCGAGCAAGGTGGTTC‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTGTGCCAGCGGCGTCCCCTGATTGa                               <  2:222372/249‑1 (MQ=255)                                                                                                                                                                                                                         aTCGCCGCATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTgc                               >  2:8983/1‑250 (MQ=255)                                                                                                                                                                                                                                cATAACGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTa                        <  1:80543/251‑1 (MQ=255)                                                                                                                                                                                                                                 aTAACGTGCCGAGCAAGGAGGTCT‑CCCCGCCCCCTTCGCGCCAATCAGCGTGACAATCTCGCACTGCTAGATATGCCGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTCTTACGGATCTGCTCCGGCGTACCGTTGGCCAGCGGCGTCCCCTGATTGACCACGTa                        >  2:402530/1‑250 (MQ=255)                                                                                                                                                                                                                                   accGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAAt                     <  1:204545/249‑1 (MQ=255)                                                                                                                                                                                                                                   aaCGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAAt                     <  1:64953/251‑1 (MQ=255)                                                                                                                                                                                                                                    aCGTGCCGAGCAAGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAAtt                    <  1:240653/251‑1 (MQ=255)                                                                                                                                                                                                                                     cgggCCGAGCAAGGTG‑TTTCCCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTGGCCAGCGGCGTCCCCTGATTGACCACGTAAATTc                   <  2:57740/248‑1 (MQ=255)                                                                                                                                                                                                                                               aaGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAAt         >  2:317415/1‑251 (MQ=255)                                                                                                                                                                                                                                                aGGTGGTTT‑CCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGAGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCCGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGaactt        >  2:123537/1‑248 (MQ=255)                                                                                                                                                                                                                                                      gtttCCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCAt   >  1:225821/2‑251 (MQ=255)                                                                                                                                                                                                                                                       tttCCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATc  >  1:289013/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         GAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCC‑ATCGCCAGGTTCTCTTCCACCGTCATCCGCGAGAAGACGCGACGCCCTTCCGGGACAATCGCCACCGCTTCGCGCA‑TGATTTTC‑GCTGTCTGCCAGTCGGTAATG‑TCTT‑TATCATCAAACACAATTCGCCCGCTGGTGGCACGCGGATCGCCGCATAACGTGCCGAGCAAGGTGGTTTTCCCCGCCCCGTTCGCGCCAATCAGCGTGACAATCTCGCCCTGATTGATATGCAGGCTCACCTCATGCAGCGCCTGGATTTTGCCGTAGTGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATC  >  NC_000913/3593065‑3593556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑