| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 723,528 | (T)8→7 | coding (887/2685 nt) | kdpD ← | sensor histidine kinase KdpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 723,528 | 0 | T | . | 98.3% | 266.7 / ‑4.5 | 60 | coding (887/2685 nt) | kdpD | sensor histidine kinase KdpD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (35/24); minor base C (0/1); total (35/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCATCAAGCGCGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGA > NC_000913/723286‑723773 | gtTCATCAAGCGCGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCCGAAAGGGTTGCCGTCTCCGCGCCCAGGTCCTGCGCCAGACGTAAGGCGCGGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGc > 2:91485/1‑251 (MQ=255) aGCGCGACCAGCACCTGGTCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCGCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTGTTTT‑CCGGTAAGCGGGgcagg < 2:430308/251‑1 (MQ=255) cAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAACTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGt > 1:76791/1‑251 (MQ=255) aCCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCTCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTGCTCTTCCGCTGGATCAGAAAGTGTTGCCGGCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGGCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCa < 1:296046/251‑1 (MQ=255) aCCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTc > 1:261866/1‑250 (MQ=255) gATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGACAGTGTTGCCGTCTACGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCCGGGCAGGGGTTTCAACATCCACCGCGTGCCAGAc > 2:300325/1‑251 (MQ=255) gcgcgcCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCt > 1:259560/1‑251 (MQ=255) gcgcCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCGGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTAc < 1:68292/251‑1 (MQ=255) gcgcCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTAc > 1:192433/1‑251 (MQ=255) gTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGcc < 1:394546/251‑1 (MQ=255) gttCAGCAAACTTTTCCCGACGCCACCGGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTg < 2:320833/249‑1 (MQ=255) ggTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGAGACCGAGAATAATCTTGCCGAGCTTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGCTAGGGGTTTCACCATACATCGCGTGCAAGACGCTAccccaccgtg > 2:78599/1‑244 (MQ=255) gaGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGGGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGGGCCAGACGCTACCCAGCCGTGACg < 2:413547/250‑1 (MQ=255) aaaCGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGCCGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGcc > 2:366950/1‑249 (MQ=255) aaaCGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAg < 1:382803/251‑1 (MQ=255) aCGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgcc < 1:104854/251‑1 (MQ=255) aCGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgcc < 1:366950/251‑1 (MQ=255) cccGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTACGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGTGTGCCAGACGCTACCCAGCCGTGACGCCAgccgcgc > 2:336793/1‑249 (MQ=255) cgccGCCACCAGCGGCGCGAGGCCGGGCACCCGAGAAGAATCTTGCCGAGATAATGTTCACGGGCATAACGCACTCCCGCTTGCTCTTCCGTTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCATTTCCTGCGCCAGACGTAAGGCGCGGAGAATTGCCCGGCGTTTTTTT‑CTGGTAGGCGGTGCAGGGCAGGGGGTTCAACATACACCGCTTGCCAGACTCTACCCAGCCGTAACGCCAGCCTCGCCGCt < 2:294432/248‑1 (MQ=255) cgtcaaccacgggcgcggtgCCGGGCTACCGGGAATAAGTGTGCCGAGATTATGTGCCCGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGAGCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGTTAAGCGGTGCAGGGCAGGGTTTGCAACAGACCCCGCGGGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTgc < 2:208163/232‑1 (MQ=255) gCCACCAGCGGCGCGAGGCCGGGCGCCCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGCCGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTgcgc > 2:384239/1‑251 (MQ=255) cgAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTc < 2:259560/251‑1 (MQ=255) cGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGAACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTCCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCggcg > 1:44533/1‑249 (MQ=255) ccGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGtt > 2:353453/1‑250 (MQ=255) cGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGCCGTAAGGCGCTTAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCCGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTAt > 1:286719/1‑251 (MQ=255) gaATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGGGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGGGTTATGGc > 2:9096/1‑251 (MQ=255) taatCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCg > 2:197049/1‑250 (MQ=255) tGCCGAGATTATGTTCACGGGCAAAACGCACTACCGCTTTCTCTTCCGCGGGATCAGAAAGTGTGGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAACTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGc < 2:76791/248‑1 (MQ=255) agaTTATGTTCACGAGCATAACGCTCTACAGCTTTCTCTTCCGCTGGATCAGCAAGAGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAACCGGTGCAGGGCAGGGGTTTCAACCTACACCGCGTGCCAGACGCTACCCAGCCGGGACGCCACCCGCGCCGCTGCGCCGCCCAGTTTTTCGCTCCCGGTGTTATGGCCGATGCATAAAAgg > 2:87409/1‑251 (MQ=255) gTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGAtcgcg < 1:384239/250‑1 (MQ=255) gTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAAGAtcgcgg > 2:323947/1‑250 (MQ=255) cACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGAtcgcgtcgc > 1:306506/1‑251 (MQ=255) ggCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGACTTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCgt > 1:272118/1‑251 (MQ=255) cATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAATGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCgtgt < 1:58860/251‑1 (MQ=255) gCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCcac > 1:11325/1‑250 (MQ=255) aCTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGGTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACt < 1:115764/251‑1 (MQ=255) tACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACttt > 1:394990/1‑251 (MQ=255) cccatttgctctCCCGCGGGTTCAGAAAGTGTTGCCGTGCCCGCGCCCAGTGCCTGCGCCAGACGGAAGGCGCTCAGAATTGCCCGCCGTTTTGTT‑CCGGTAAGCGGTGCAGGGCAGGGTTTGAAACATGCACCGCGTGCCAGACGCTTCCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGTACCAGTGTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGtgtgtgcccctttc < 2:132983/243‑11 (MQ=255) cccGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACttt < 2:360555/249‑1 (MQ=255) ttCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTc < 1:375524/250‑1 (MQ=255) tctcTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCAGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGc > 2:373810/1‑251 (MQ=255) tctTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTCTGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGcc > 2:314734/1‑250 (MQ=255) cttccgatggacccgaacgcgctGCCGTCACCGCGCCCCGTTCCTGCGCCAGACGTAAGGCGCTGAGCATTGCCCGACGTTTTTTT‑CCGGTAAGCGGCGCAGGGCAGGGGCTTCAACATACACCGCGTGCCAGACGCCACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGAGCCACACTTTCTCTTCGcc > 2:389934/23‑249 (MQ=255) cTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCCTAAAAGGATCGCGTCGCGCGTGTGCCACCCTTTCTCTTCGCCCGGATGcc > 2:43634/1‑251 (MQ=255) aGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGCAAGCGGTGCAGGGCCGGGGTTTCAACATACACCGCGTGCCAGACGCTGCCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGCTCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGCTGCCCCC‑Gcc > 2:26634/1‑250 (MQ=255) gAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑Gcc > 2:45989/1‑249 (MQ=255) aaaGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGGCGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAgg > 1:432504/1‑251 (MQ=255) aaaGTGTTGCCGACTTCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGCGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCATGGCAGGGGTTTCAACATACACCGAGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCAGACTAGTTTTTCGCTGCCGGTGTTATGTCCGATGCATAATATGATCGCGTCGAGCGTCTGCCACCCTGTTTATTCGCCAGGATTCCCCC‑GCCAgg > 2:19499/1‑251 (MQ=255) aagtttggcctgccccggccccAGTGCCTGCGCCAAACGTAAGGCGCTGAGAATTGCCCGACGTGTTTTC‑CCGGTAAGCGGTGCAGGGCAGGGGTTACACCATACACCGCGTGCAAGACGCTACCCATCCGGGACGCCAGCCGCGCCGCTGCGCGGACCAGGTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAGgc < 2:138358/232‑1 (MQ=255) aGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGACGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAGgcg > 1:385160/1‑251 (MQ=255) gcCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGACCGCGTCGCGCGTGTGCCCCACTTTCTCTTCGCCAGG‑TGCCCCCCGCCCGGCGCGCATTTGCtcatccac > 2:239390/1‑248 (MQ=255) ccaggtgccgccgccggccGTAGGGCGCTGAGAATTGCCCGCCGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTCCAACATACACCGCGTGCCAGACGCTCCCCAGCCGTGAGGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAGGCGCGCATTTGCTCATCAAcg < 2:357518/233‑1 (MQ=255) cAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGcc > 1:415716/1‑152 (MQ=255) cAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTGTGTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGTACCAGTTTGTCGCTGcc < 2:415716/152‑1 (MQ=255) ccAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTAc < 1:16040/251‑1 (MQ=255) gTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGAAAGCGGTGCAGGGCAGGGGTTTCACCATACACCGCGTGCCACACGCTACCCAGCCGTAACGACAGCCGAGCCGCTGCGCGGACCAGCTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGAACGCGTCGCGCGTGTGCCCAACTTTCTCTTCGCCAGGATGCACCC‑GCCACGCGCGCATTTGCTCAACCACGCAAACGGCATTACGgcgc > 2:166899/1‑250 (MQ=255) cgcTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGcc > 1:239857/1‑250 (MQ=255) ggagaATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGtt < 1:323947/250‑1 (MQ=255) ccccGAC‑TTTTTTTCCCGGTAAGCGGGGCAGGGCAGGGTTTCCAACATCCCCCGCGTGCCAGACGCTCCCCAGCCGGGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAgg < 2:394990/250‑1 (MQ=255) cccGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAGGCGCGCATTTGCTCATCAACGAGATCGGCAGTACGGCGCAGGGCCAGTTCGCGCCggg > 2:307768/1‑251 (MQ=255) ttttttCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCCGGCGCGCCTTTGCTCATCAACGCGATCGGCCGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAgg > 2:401721/1‑250 (MQ=255) | GTTCATCAAGCGCGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCC‑GCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGA > NC_000913/723286‑723773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |