BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,241,235	A→G	G340G (GGT→GGC)	xylE ←	D‑xylose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,235	0	A	G	97.5%	415.8 / ‑6.1	123	G340G (GGT→GGC)	xylE	D‑xylose:H(+) symporter
Reads supporting (aligned to +/- strand): ref base A (0/1); major base G (59/60); minor base T (1/2); total (60/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 123 total aligned reads displayed.

GAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGG  >  NC_000913/4240992‑4241478

gAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGACAGCGATTTACCACGAATAGCATTCGGGAAGATATCCGCAAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCAGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAAtt                                                                                                                                                                                                                                              >  1:901363/1‑251 (MQ=255)

 aaGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGACTAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAAttt                                                                                                                                                                                                                                             >  2:99450/1‑251 (MQ=255)

            cagccaCTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGCTAATTTGCAGCGGCTTACGGCCAAAtt                                                                                                                                                                                                                                              >  2:310885/1‑239 (MQ=255)

            cagccaCTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTc                                                                                                                                                                                                                                   >  1:701445/1‑250 (MQ=255)

            cagccaCTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTc                                                                                                                                                                                                                                   >  2:694474/1‑250 (MQ=255)

            cagccaCGGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCa                                                                                                                                                                                                                                  <  2:12029/251‑1 (MQ=255)

                 ccTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGGACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAAtt                                                                                                                                                                                                                             <  1:909867/250‑1 (MQ=255)

                 aCTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAAtt                                                                                                                                                                                                                             >  2:736337/1‑251 (MQ=255)

                   tGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACACTACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCCGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGc                                                                                                                                                                                                                           >  2:42434/1‑251 (MQ=255)

                    gggCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTg                                                                                                                                                                                                                            <  1:790523/249‑1 (MQ=255)

                        cgccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTACAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAAtttcccat                                                                                                                                                                                                                       >  2:34000/1‑244 (MQ=255)

                            aCCGCGATTGCCAGCGCTTTACCCCGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGATTACGGCCACATTTATCCACCGTCATAATTGCCAGAACGGt                                                                                                                                                                                                                  >  2:567254/1‑251 (MQ=255)

                             ccGCGAGTTCCAGCGCTTTACAACGAAAAGCATTCGGGAAGATTTCCGGCAGCAGTACCCATCATACCGGACCCCATGACATGGCAAAGGCGGCAACATATAACAGCATCGACAGGAGCGCCACAAGACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCGAAACATACCGATTGCCATTCCGAGTGCGCCTATAATTGGCAGTGGCGGACGGCGAAATTTATCCACTGTCAAAATTGCCAGAACGGTg                                                                                                                                                                                                                 <  2:422535/251‑1 (MQ=255)

                                 gATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAgg                                                                                                                                                                                                             >  1:332232/1‑251 (MQ=255)

                                  aTTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGt                                                                                                                                                                                                            <  1:285696/251‑1 (MQ=255)

                                     gCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTg                                                                                                                                                                                                           >  1:470445/1‑249 (MQ=255)

                                       cAGCGCTTTACCACGAATAGCATTCGGGACGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCAAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGt                                                                                                                                                                                                       >  1:771099/1‑251 (MQ=255)

                                       cAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGACATAATTGCCAGAACGGTGAAGGTGAGGt                                                                                                                                                                                                       <  1:317787/251‑1 (MQ=255)

                                       cAGCGCTTTACCACGAATAGCATTCGGGAAGATTGCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTGACGGCCAAATTGATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGt                                                                                                                                                                                                       <  2:439850/251‑1 (MQ=255)

                                          cgcTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGAGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGa                                                                                                                                                                                                    <  1:636094/251‑1 (MQ=255)

                                           gcTTTAACACGAATAGAATTCGGGCAGATTTCAGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGCCAACCTAGCACACCATCGACCGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGCGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCCAATTTATCCACCGTCATAATTGCCAGAACGGTGACGGTGCGGTTGAt                                                                                                                                                                                                   >  2:601180/1‑251 (MQ=255)

                                                  cACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCg                                                                                                                                                                                            >  1:728512/1‑251 (MQ=255)

                                                     gAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGAc                                                                                                                                                                                          >  1:234398/1‑250 (MQ=255)

                                                     gAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAAGTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACa                                                                                                                                                                                         <  1:576045/251‑1 (MQ=255)

                                                        tAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACaat                                                                                                                                                                                       >  1:635528/1‑250 (MQ=255)

                                                            aTTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTTCCGAGGCTAAACATACCGATTGCCATGCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAAGTGCCAAAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATgg                                                                                                                                                                                  <  1:601180/251‑1 (MQ=255)

                                                            aTTCGGGAAGATTTCCGACAGAAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATgg                                                                                                                                                                                  >  2:856049/1‑251 (MQ=255)

                                                               cGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCt                                                                                                                                                                               <  2:728512/251‑1 (MQ=255)

                                                                gggAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTg                                                                                                                                                                              <  1:42434/251‑1 (MQ=255)

                                                                 ggAAGATGTCCGACAGCAGGACCCAGCATACCGCACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGc                                                                                                                                                                             <  2:470445/251‑1 (MQ=255)

                                                                    aGATTTCCGCAAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATCCCGAATGCCATTCCGAGTGCGCCGATAATTTTCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGTAGGTGAGGTTGATAACTCCGACAATAATGGTCttcagc                                                                                                                                                                          <  2:686567/251‑6 (MQ=255)

                                                                      atttgggACATCAGGACCCAGAATACCGGACCCCTGGACATGGCTATGGCGCCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGCTGCCTGAGGGGAAAACGGGGTACGGAGGCTAAACTAACCGATTGCCAATCCGAGTGCGCAGATAATTTGCACTGGCTTACGTCCCAATTTATCCACCGTCATAATCGCCCGGACGGTGAAGCAGAGGTTGTTAACTCCGACaagcttggcctgcagc                                                                                                                                                                          >  2:14915/7‑235 (MQ=255)

                                                                      aTTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACCGCATAGACACTAGCGCCACCATACCCGGTGCATGAGTGTAAAAAGCGGCACCGAGGCTAACCATCCCGATTGCCATTCCGAGTGAGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAAc                                                                                                                                                                          >  1:931351/1‑249 (MQ=255)

                                                                        gtCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACAGACCGATTGCCAGTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCGAAAGTTATCCACCTTCATAATTCCCAGAACGGTGAAGGTGATGCTGATATCCCCAACAAGAACGGTCTGCAACAgc                                                                                                                                                                       <  2:434603/249‑1 (MQ=255)

                                                                               agcagTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATgaattggtatcgcgcaaacgc                                                                                                                                                               >  2:671322/1‑231 (MQ=255)

                                                                                gcagTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATcc                                                                                                                                                              <  1:736337/251‑1 (MQ=255)

                                                                                  agTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGt                                                                                                                                                            <  2:635528/251‑1 (MQ=255)

                                                                                      cccAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTTCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTg                                                                                                                                                        <  1:337487/251‑1 (MQ=255)

                                                                                         aGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTGGCAGTTGCTTACGTCCAAGGTTATCCGCCGTc                                                                                                                                                                                                                                   <  2:897859/173‑1 (MQ=255)

                                                                                         aGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGACACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTc                                                                                                                                                                                                                                   >  1:897859/1‑173 (MQ=255)

                                                                                         aGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAAAAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGcc                                                                                                                                                     >  1:547994/1‑251 (MQ=255)

                                                                                           cATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGCGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACaa                                                                                                                                                                                        >  2:791777/1‑214 (MQ=255)

                                                                                           cATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGAGAACTCCGACaa                                                                                                                                                                                        <  1:791777/214‑1 (MQ=255)

                                                                                            ataacGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGGGAGGTTGATAACTCCGACAATAATGGTCTGCAACCGCGCGATATCCGTGCTGGccccc                                                                                                                                                  >  2:903475/5‑251 (MQ=255)

                                                                                             tACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGCGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGccccc                                                                                                                                                  >  1:659393/1‑250 (MQ=255)

                                                                                              aCCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGccccc                                                                                                                                                  >  1:647880/1‑249 (MQ=255)

                                                                                              aCCGGACCCCAGGACATGGCAAAGGCGGCAACAGAGAACAGCATCGACAGTAGCGCCACGATACCCGGCGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGTCGATAATTTGCAGGGGTTTACGGCCCAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAg                                                                                                                                                                        <  2:284378/227‑1 (MQ=255)

                                                                                              aCCGGACCCCAGGACATGGCAAAGGCCGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGGGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGGAGGTGAGGTTGATAACTCAGACAATAATGGTCTGCAACAg                                                                                                                                                                        >  1:284378/1‑227 (MQ=255)

                                                                                                 ggACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGt                                                                                                                                             <  2:234539/251‑1 (MQ=255)

                                                                                                   aCCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGttt                                                                                                                                           >  1:570197/1‑251 (MQ=255)

                                                                                                         ggACAGGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATTCCCGGTGCCTGAGTGTAAAACTCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACTGTGAATGTGAGGTTGATAACGCCGACAATAAGGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAaca                                                                                                                                     <  2:945110/251‑1 (MQ=255)

                                                                                                          gACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAacac                                                                                                                                    >  1:60372/1‑251 (MQ=255)

                                                                                                            cctGGCAAAGGCAGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGGGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTGGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACtt                                                                                                                                  <  2:227967/249‑1 (MQ=255)

                                                                                                                gCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATCCCCGGTGCATGAGTGTCAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGACGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGcc                                                                                                                                                     >  1:941682/1‑228 (MQ=255)

                                                                                                                gCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGATTGTAAAACGCGGTACCGAGGCTAAACATACCGATGGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCATCAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCgg                                                                                                                              <  2:230852/251‑1 (MQ=255)

                                                                                                                gCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGAGTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCTACAATAATGTTCTGCAACAGCGCGATATCCGTGCTGGcc                                                                                                                                                     <  2:941682/228‑1 (MQ=255)

                                                                                                                  aaaGGCGGCAACATAGAACGGCAGCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCGAAACATAACGATGGCCATTCCGAGTACGCCGATAATTTGCAGTGGCTTACGGCCAAATTTAGCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACCCCGACAAAAGTGGTCTGCTACAGCGCGTTATCCGTGCGGTCCCCCAGCGTTTTGGACACTTCCGgcg                                                                                                                            <  2:497556/251‑1 (MQ=255)

                                                                                                                        ggcAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCgtagt                                                                                                                      >  2:693642/1‑251 (MQ=255)

                                                                                                                           aaCATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGAGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTAc                                                                                                                    >  1:94053/1‑250 (MQ=255)

                                                                                                                              aTAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGc                                                                                                                 >  1:679571/1‑250 (MQ=255)

                                                                                                                                  aaCAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCGTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCTCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGcaccac                                                                                                            <  1:626215/251‑1 (MQ=255)

                                                                                                                                    cAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACAt                                                                                                          <  1:132488/251‑1 (MQ=255)

                                                                                                                                           gACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGcc                                                                                                   >  1:807250/1‑251 (MQ=255)

                                                                                                                                              aGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGt                                                                                                                                             <  1:278113/206‑1 (MQ=255)

                                                                                                                                              aGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGt                                                                                                                                             >  2:278113/1‑206 (MQ=255)

                                                                                                                                               gTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACGCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACa                                                                                               <  1:874456/251‑1 (MQ=255)

                                                                                                                                                          aTACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTACGCCGATAATTTGCATTGGCTTACGGCCAAATTAACCCCCCGTCATACTTGCCAGAACGTTGACGGAGAGGTTGATAACTCCGACAATAATGGTCTGCAAAAGCGCGATATCCGTCATGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGAACCCCTTTGATGCCGACAAATTGCTGGa                                                                                     >  2:39983/1‑250 (MQ=255)

                                                                                                                                                              ccGGTGCCGGCGTGTAAATCGCGGTACCGAGGCTAACCTTACCGAATGCCATTCCGAGTGCGCCGTTAATTTGCAGTGGCTTACGGCTAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGTTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGATCACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATg                                                                                <  2:562594/251‑1 (MQ=255)

                                                                                                                                                                 gTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGata                                                                                                                                                                 >  2:363960/1‑167 (MQ=255)

                                                                                                                                                                 gTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGata                                                                                                                                                                 <  1:363960/167‑1 (MQ=255)

                                                                                                                                                                    ccTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCACATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGc                                                                          >  1:68405/1‑251 (MQ=255)

                                                                                                                                                                    ccTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGc                                                                          <  2:647880/251‑1 (MQ=255)

                                                                                                                                                                       gAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGAGGGAGAGCa                                                                         <  2:807250/249‑1 (MQ=255)

                                                                                                                                                                        aGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTcc                                                                                                                                >  1:677997/1‑193 (MQ=255)

                                                                                                                                                                        aGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCAtt                                                                       >  1:94823/1‑250 (MQ=255)

                                                                                                                                                                         gtgtAAAACGCGGTACCGAGGCTAAACATACCGAGTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTAc                                                                     <  1:99491/251‑1 (MQ=255)

                                                                                                                                                                          tgtAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGACGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACg                                                                    >  2:406630/1‑251 (MQ=255)

                                                                                                                                                                              aaaCGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCg                                                                 >  1:303394/1‑250 (MQ=255)

                                                                                                                                                                               aaCGCGGTACAGAGGCTAACCATACCGATTGCCATTCCGAGTGCGCGGACAATTTGCAGTGGCTTAAGGCCAACTTTCTCCACAGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCAAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGAt                                                               >  2:403026/1‑251 (MQ=255)

                                                                                                                                                                                     gTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAAt                                                         <  1:722699/251‑1 (MQ=255)

                                                                                                                                                                                        ccGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATcac                                                      <  1:406630/251‑1 (MQ=255)

                                                                                                                                                                                                taaCATACCGATTGCCATTCCGAGGGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATcacgcccacg                                               <  2:679571/249‑1 (MQ=255)

                                                                                                                                                                                                aaaCATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATcacgcccac                                                <  1:693642/249‑1 (MQ=255)

                                                                                                                                                                                                  aCATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGGGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCa                                            <  1:453047/251‑1 (MQ=255)

                                                                                                                                                                                                     tACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGAGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGCTGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAAc                                         >  2:778219/1‑251 (MQ=255)

                                                                                                                                                                                                      aCCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAAc                                         >  1:91520/1‑250 (MQ=255)

                                                                                                                                                                                                      aCCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGGGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAAc                                         >  1:187833/1‑250 (MQ=255)

                                                                                                                                                                                                            tGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCACATTTATCCCCCGTCATAATTGCCAGAACGGTGCAGGTGCGGTTGATACCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATcagc                                   >  1:99636/1‑250 (MQ=255)

                                                                                                                                                                                                            tGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGc                                                                   >  1:344431/1‑218 (MQ=255)

                                                                                                                                                                                                             gCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGCGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATcagc                                   >  1:558013/1‑249 (MQ=255)

                                                                                                                                                                                                             gCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGGACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACTCCCACGCCAAACATcagcag                                 <  2:570197/251‑1 (MQ=255)

                                                                                                                                                                                                                  tCCGAGTGCGCCGATAATTTGCAGTGGCTGACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCTCCACATGGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAgacga                             <  2:94053/250‑1 (MQ=255)

                                                                                                                                                                                                                   ccGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGacc                           <  1:181522/251‑1 (MQ=255)

                                                                                                                                                                                                                     gAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGaccac                         >  1:895640/1‑251 (MQ=255)

                                                                                                                                                                                                                      aGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGaccacc                        >  1:506350/1‑251 (MQ=255)

                                                                                                                                                                                                                            ccGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGAt                                                               <  2:135031/206‑1 (MQ=255)

                                                                                                                                                                                                                            ccGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGAt                                                               >  1:135031/1‑206 (MQ=255)

                                                                                                                                                                                                                             cGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCCGACGCCCACCGGTTTTg                 >  2:733529/1‑251 (MQ=255)

                                                                                                                                                                                                                                  aTTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATCATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACCTTGATGCCGACAAATTGCTGGAAGATGAAGAGCATTACGCCGATTCCAATCACGCCCACGCCAAACATCAGCAGACGCCCACCGGTTTTGCGGcc            >  1:493838/1‑251 (MQ=255)

                                                                                                                                                                                                                                       cAGTGGCTTACGGCCAAATTTATCCACAGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGc                >  1:806679/1‑242 (MQ=255)

GAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGG  >  NC_000913/4240992‑4241478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`