Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,204,890 C→T G348S (GGT→AGT)  purD ← phosphoribosylamine‑‑glycine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,204,8900CT99.2% 477.1 / NA 132G348S (GGT→AGT) purDphosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (75/55);  minor base G (0/1);  total (76/56)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

AGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGTTTGTCGATCATCAGGCCCGCGTAGAGAAAACCGGTGT  >  NC_000913/4204644‑4205135
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     
aGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTg                                                                                                                                                                                                                                                   >  1:213372/1‑251 (MQ=255)
        tCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAAt                                                                                                                                                                                                                                            >  2:730104/1‑250 (MQ=255)
        ccgcttccgacggcgcccaacccGATTTCTTCCCGGCAGAAGCAGTCTCCCCAGTGAATATGGGTCATTAAGCCATGGGCGCGTTTCTGCGCTTCGCCCAGGT‑TATGCCCCAGCGCGGTGCCGCACAGTCCCCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAAt                                                                                                                                                                                                                                            <  1:730104/229‑1 (MQ=255)
          gcgTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCAc                                                                                                                                                                                                                                         >  1:713486/1‑251 (MQ=255)
            gTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCAccc                                                                                                                                                                                                                                       >  1:931915/1‑251 (MQ=255)
            gTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGAGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCAccc                                                                                                                                                                                                                                       >  1:566149/1‑251 (MQ=255)
                gATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGCTCACGTCACTGGTGCGGTAATCACCCGGat                                                                                                                                                                                                                                   >  2:173343/1‑251 (MQ=255)
                     cgcgcCATCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATcc                                                                                                                                                                                                                               >  1:200638/1‑250 (MQ=255)
                       ccccAGCCGATATCTTCCCGGAAAAATCAGTCGCCCCAGTGGATATCGTTAATTAAGGCATGGGCGCTTTCCTGCCCTTCTCCCCCGT‑TATGCCCCAGGGGGGGGCCCCACATTCCCCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAAGCACCCGGATATCCAcc                                                                                                                                                                                                                            <  1:350446/249‑1 (MQ=255)
                        gcCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCAccc                                                                                                                                                                                                                           >  1:39050/1‑251 (MQ=255)
                              cGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCCCTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGcc                                                                                                                                                                                                                     >  1:50409/1‑251 (MQ=255)
                                  atCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATc                                                                                                                                                                                                                  >  1:196645/1‑250 (MQ=255)
                                      ttcgggCAGAAGCAGTCGCCCCGGGGGATAGCGGTATTTAAGGCATAGGCGCGTTTCGGCGCTCCGGCCCCGT‑TAGGCCCCGGCGCGGTGCCGCAAAGTCCGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATcac                                                                                                                                                                                                                <  1:173343/244‑1 (MQ=255)
                                         cGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGcc                                                                                                                                                                                                          >  1:319388/1‑251 (MQ=255)
                                            aaaaaGCGGTCGCCCCGGTGGATAGCGGTCATTAAGGCATAGGCGCTTTTCTGCGCTTC‑GCCCCGGGTAGCCCCCGGGGGGGGGCCGCCAAGTCCGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCgag                                                                                                                                                                                                       <  1:298701/248‑1 (MQ=255)
                                                 gaaGTCGCCCGAGTGGATACGGGTAATTAGGGAAGAGGCGCGTTCCGGCGCTTCTCCCCGGG‑TAGGACCCGGCGGGGTGACGCACAGAACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAg                                                                                                                                                                                                  <  1:733857/249‑1 (MQ=255)
                                                   aGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCCCTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAgcg                                                                                                                                                                                                >  2:208160/1‑251 (MQ=255)
                                                          ccAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGACACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAgc                                                                                                                                                                                                 >  1:579652/1‑243 (MQ=255)
                                                           cAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCCCGG‑CATGACCCAGCGCGGTCACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTAGTCC‑TCCGCCAGTTTTGTGACCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGACCGTGGACCACGTCACTGGTGCGGTCATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATc                                                                                                                                                                                        >  1:600363/1‑251 (MQ=255)
                                                             gTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATccc                                                                                                                                                                                      >  1:660811/1‑251 (MQ=255)
                                                              tGGATATCGGTCATTAAGGCATAGGCGCGTTTATGCGCTTCTGCCACGG‑TATGACCCAGCCCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATACACCCGAAGCCCTCACCACGCCGAGAGCAGCGCGTTCATcacc                                                                                                                                                                                     >  2:362419/1‑248 (MQ=255)
                                                                 atatcgttaattagggcaggggCGCGTTTCTGCGCTTCGGCCCCGG‑TATGACCCAGGGCGGTGACCCAAAGTCCGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTc                                                                                                                                                                                  <  1:116889/231‑1 (MQ=255)
                                                                 atatCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACACAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCCCTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGCGAGACGCGCGTTCATCCCAccc                                                                                                                                                                                  >  2:664288/1‑249 (MQ=255)
                                                                  tatCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCg                                                                                                                                                                                 >  1:222627/1‑251 (MQ=255)
                                                                     ggttcataaagcaatgggCGCTTTTCTGCGCTCCTGCCCCGT‑TGTGCCCCAGCGGGGTGCCGCACATGCCGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑CCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACTTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGAc                                                                                                                                                                              <  1:160270/235‑1 (MQ=255)
                                                                     cGGTCATTCAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCAATACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCCCTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCCTCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGAc                                                                                                                                                                              >  2:86358/1‑251 (MQ=255)
                                                                       gTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACACGGACGt                                                                                                                                                                            >  2:697051/1‑251 (MQ=255)
                                                                          aTTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGt                                                                                                                                                                                                                                               >  1:834680/1‑181 (MQ=255)
                                                                          aTTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATAACCCAGCGCGATGCCGCACAGAACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCC‑TCCGACAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTCCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGtttt                                                                                                                                                                         >  1:120394/1‑251 (MQ=255)
                                                                                 cATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTcc                                                                                                                                                                  >  1:84764/1‑251 (MQ=255)
                                                                                        gcgTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGCCGCACAGAACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGc                                                                                                                                                           >  1:879051/1‑251 (MQ=255)
                                                                                        gcgTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGCGAAGCGCGTTCATCCCACCCGGACGTTTTCTCGTCCAGTTTGc                                                                                                                                                           >  2:169993/1‑251 (MQ=255)
                                                                                            ttCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCCTCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTc                                                                                                                                                       >  2:110674/1‑251 (MQ=255)
                                                                                                 cgttcctcccACGG‑AATGTCCCAGCGGGGTGCCGCACTGT‑CCCGTCCCGCCGTTGGTCTCTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAgg                                                                                                                                                  <  1:258102/243‑1 (MQ=255)
                                                                                                   cTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGgcc                                                                                                                                                >  1:258489/1‑251 (MQ=255)
                                                                                                      cTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCACCAGTTTTGTGCCCGCGTGG‑AACAATTTGCCGCCTGCCACTTCTTACAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGCTTTCTCGTCCAGTTTGCTTTCACAGgcagcc                                                                                                                                             >  1:499044/1‑251 (MQ=255)
                                                                                                         ccACGG‑AATGACCCAGAGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGCCCCTCCCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGttt                                                                                                                                                                          >  2:892181/1‑219 (MQ=255)
                                                                                                             gg‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGc                                                                                                                                         >  2:277130/1‑248 (MQ=255)
                                                                                                                     cccagccaggTGAGGCACAGTACGCG‑CCCGACGTTGGTCACTACCTG‑CTCGACA‑TCCGCCAGTTTTGTGCCCGCGTGG‑ACCACTTTGCCGCCTGCCACTTCTTCCAGAGGCAGGCCGTGGATCCCGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCCGACGTTTTCTCGTCCAGTTTTCTTTCACCGGCCGCCAGGCCGAGCCCAAc                                                                                                                               >  2:851994/9‑251 (MQ=255)
                                                                                                                     cccAGCGGGGTGACGCACGGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAAc                                                                                                                               <  1:593113/251‑1 (MQ=255)
                                                                                                                           gcgGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAAcc                                                                                                                              >  1:88870/1‑246 (MQ=255)
                                                                                                                            gtgtggccctccgtaccgccccccccgTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACTCTTTGCCGCCGGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATc                                                                                                                         <  1:162752/227‑1 (MQ=255)
                                                                                                                            gcgggccgcactgtccgtcccccgccGTGGGTCACTACCGG‑CTCGTCA‑CCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTTCTTGCACAGGCCGCCAGGCAGAGCTCAACCAGATc                                                                                                                         <  2:387953/231‑1 (MQ=255)
                                                                                                                            cggcgACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGCCA‑TCCGCCCGTTTTGCGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCCGGCCGTGGATCACGTCACTGGTGCGGTAATCAACCGGATATCCACCCGCAGCCATCAACACCCCGCGAGAAGCGCGATCATCCCCCTCGGCCGCTTTCACGTCCCCTCTGCTTTCCCAGGCCGCCCGGCAGAGCTCaccc                                                                                                                              >  2:647037/5‑242 (MQ=255)
                                                                                                                            cgGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAAcc                                                                                                                              >  1:298229/1‑245 (MQ=255)
                                                                                                                               cctccccgcggctcttccgatctgTTGGTCACTACCTG‑CTCGTCA‑CCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTGCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGa                                                                                                                      <  2:286384/228‑1 (MQ=255)
                                                                                                                                 acctcactgtccccccccgccGTTGGTCTCTTCCGT‑CTTGTCA‑TCCGCCAGTTTTGTCCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGATAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCATATCGGACt                                                                                                                    <  1:907349/236‑1 (MQ=255)
                                                                                                                                   cccacggtccgcccccgccGTTGGTCCCTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTc                                                                                                                  <  1:277130/238‑1 (MQ=255)
                                                                                                                                   ccacgacccccgcccggcctttGGTCACTCCCTG‑CTC‑CCACTCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACGGGGGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTc                                                                                                                  <  2:931915/231‑1 (MQ=255)
                                                                                                                                      caGTACGGG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATgc                                                                                                              <  1:85520/251‑1 (MQ=255)
                                                                                                                                           cgcg‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACCCTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCgcagc                                                                                                         <  1:174098/251‑1 (MQ=255)
                                                                                                                                           cgcg‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGGAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCCACCCGATCGGCCTTCATGCgcagc                                                                                                         >  2:32890/1‑251 (MQ=255)
                                                                                                                                            gcg‑cCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCgcagca                                                                                                        <  1:281152/251‑1 (MQ=255)
                                                                                                                                             cg‑cccgccGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCa                                                                                                                                            >  2:267029/1‑214 (MQ=255)
                                                                                                                                              g‑cccgccGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTGGTCCAGt                                                                                                                                                               >  1:184311/1‑194 (MQ=255)
                                                                                                                                              g‑cccgccGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTCTGCCCGCGTGG‑CACACTTTGCCGCCTGCAACTTCTTCCAGCGGCAGGACGTGGATCACGTCCCTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGCCGTTTTCTCGTACAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCAt                                                                                                       >  1:854844/1‑250 (MQ=255)
                                                                                                                                                   cccgTTGGTCACT‑CCTGGCTCGTCA‑CCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCg                                                                                                  <  2:319388/250‑1 (MQ=255)
                                                                                                                                                     cggtggtgccctacaggttcgccA‑TCCGCCATTTTTGTGCCCGGGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTGCACAGTCCGCCAGGCAGAGCTCAACCAGATCGGACGTCATGCGCAGCATAATCgg                                                                                                 <  2:578455/229‑1 (MQ=255)
                                                                                                                                                     cgTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATc                                                                                                   >  1:341973/1‑248 (MQ=255)
                                                                                                                                                      gtgggTCACTACCTT‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACCCTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCt                                                                                               <  2:301676/248‑1 (MQ=255)
                                                                                                                                                      gTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGa                                                                                                                      >  1:286384/1‑228 (MQ=255)
                                                                                                                                                      gTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCt                                                                                               >  1:761056/1‑251 (MQ=255)
                                                                                                                                                      gTTGGTCACTACCTG‑ATCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCAATGGTGCGGTACTCACCCGGATATCCACCCGCAGCCATCACCCCGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCt                                                                                               >  2:474566/1‑251 (MQ=255)
                                                                                                                                                             aCTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCCGCATAATc                                                                                                   >  2:33849/1‑240 (MQ=255)
                                                                                                                                                             aCTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACAACGCCGAGAGAAGCGCGTTCATCCCACCCGGCCGTTTTCTCGTCCCGTTTGCTTTCACATGCCGCCAGACAGAGTCCAACCAGATCGGACTTCGTGCgcggcaacacaggatgggtttc                                                                                        >  2:279481/1‑235 (MQ=255)
                                                                                                                                                              cctcccggcgcgccA‑TCCGCCAGTTTTGGGCCCGCGGGG‑AACACTGTGCCGCCGTCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGttgc                                                                                        <  2:200638/238‑3 (MQ=255)
                                                                                                                                                                ccaccgtctcgtctcccGCCAGTTTTGTGCCCGCGTGG‑ACCATTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTGCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTcc                                                                                       <  2:75827/236‑1 (MQ=255)
                                                                                                                                                                 ccTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGa                                                                                    <  1:110674/251‑1 (MQ=255)
                                                                                                                                                                 ccTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGGTTCCggg                                                                                    >  2:33177/1‑250 (MQ=255)
                                                                                                                                                                       tCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCTTCCTGCCCCTTCTTCCAGCGGCAGGCCCTGGATCAAGTCCCTGGTGCGCCAATCACCCGGATATCCACCCGCAGCCATCACCACGACGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCAAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGCTTCCGGATc                                                                                  >  1:878215/1‑248 (MQ=255)
                                                                                                                                                                          ccA‑TCCGCCATTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCaaa                                                                            <  2:761056/250‑1 (MQ=255)
                                                                                                                                                                            cacccGCCAGTTTTGTGCCCGCGTGG‑AACACTTGGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGTTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCTCTCGGGCGTTTTCGCGTCCAGTTTGCTGTCACAGGCCGCCAGGCAGAGCTCATGCAGATCGGACTTCAGGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCaaa                                                                            <  1:570640/247‑1 (MQ=255)
                                                                                                                                                                              cccGCCAGTTTTGGGCCCGCGTGG‑AACCTTTTGCCCCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCg                                                                         <  2:859789/250‑1 (MQ=255)
                                                                                                                                                                                 gCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAACGCGGcc                                                                      >  2:224623/1‑250 (MQ=255)
                                                                                                                                                                                  ccAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCAt                                                                                                       >  2:218651/1‑217 (MQ=255)
                                                                                                                                                                                  ccAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCAt                                                                                                       <  1:218651/217‑1 (MQ=255)
                                                                                                                                                                                   ccttgtgttgcccggttGG‑AACACTGTGCCCCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCGCGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGGTCATCCCACGCGGACGTTTTCTCGTCCAGTTGGCTTTGACAGGCCGCCATGCATAGCTCAACCAGGGCGGACTGCATGTGCAGCATAATGGTCTGGGTTTCCGGATTGCCGAAGCGGCAGt                                                                    <  2:398726/235‑1 (MQ=255)
                                                                                                                                                                                   cAGTTTTGTGCCC‑TGTGGGAATACTGTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGAGCACGTCACTGGTGCGGTAATCACCCGGATATCCGCCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCCCTCGGACGTTTTCTCGTCCAGTTTGCTGTCACAGGCTGCCAGGCAGAGCTCAACCATATCGGACTCCATGCGCAGCATAATCGGCTGGGTTCCCGGATCGCCAAAGCGGCAGt                                                                    <  2:566149/251‑1 (MQ=255)
                                                                                                                                                                                            gCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCaa                                                           <  1:918269/251‑1 (MQ=255)
                                                                                                                                                                                            gCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCa                                                            >  1:202820/1‑250 (MQ=255)
                                                                                                                                                                                                gcgTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGTCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAAc                                                       <  1:117571/251‑1 (MQ=255)
                                                                                                                                                                                                   ggg‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCtt                                                    <  2:222627/250‑1 (MQ=255)
                                                                                                                                                                                                               cccgccTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCt                                         <  2:196645/250‑1 (MQ=255)
                                                                                                                                                                                                               cccgccTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCt                                         <  2:122734/250‑1 (MQ=255)
                                                                                                                                                                                                                 cgccTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATACACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGATAAATTCAATAACCTTCGGATTGCCCTGt                                       >  1:503434/1‑251 (MQ=255)
                                                                                                                                                                                                                   ccTGCCACTTCTTCCAGCGGCAGGCCGTGGATCCCGTCACTGGTGCGGTAATCGCCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCTGCAGTTAAATTCAATAACCTTCGGATTGCCCTGttt                                     <  2:88870/251‑1 (MQ=255)
                                                                                                                                                                                                                      gCCACTTCTTCCAGCGGCAGGCCGTGGATCCCGTCACTGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGTTTGt                                   <  1:664288/250‑1 (MQ=255)
                                                                                                                                                                                                                            tcttcCAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTGTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGGTAAATTCAATAACCTTCGGATTGCCCTGTTTGTCGatca                             <  1:547978/250‑1 (MQ=255)
                                                                                                                                                                                                                                ccAGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCCCTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCCGCATAATCGGCTGGGTTTTCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGGTTGTCGatcctacgg                        >  2:650426/1‑245 (MQ=255)
                                                                                                                                                                                                                                  aGCGGCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGTTTGTCGATCATCAGGcc                      >  1:287796/1‑251 (MQ=255)
                                                                                                                                                                                                                                     ggCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCGGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGGTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGTTTGTCGATCATCAGGCCcgc                   >  2:46906/1‑251 (MQ=255)
                                                                                                                                                                                                                                     ggCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATACAACCGCAGCCATCACCACGCCGAGAGAACCGCGTTCATCCCACTCGCACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCAAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGa                                                                                    >  2:483784/1‑186 (MQ=255)
                                                                                                                                                                                                                                      gCAGGCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCCCCACGCCGAGAGAAGCGCGTTCATCCCACTCGGAAGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCCAAGCGGCAGTTAAATTCAATAACCTTCCGATTGCCCTGTTTGTCGATCATCCGGCCcgcg                  >  2:635546/1‑251 (MQ=255)
                                                                                                                                                                                                                                         ggCCGTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATATCCTTCGGATTGCCCTGTTTGTCGATCATCAGGCCCGCGTag               <  1:851994/251‑1 (MQ=255)
                                                                                                                                                                                                                                             gTGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGTTTGTc                                  <  2:571279/228‑1 (MQ=255)
                                                                                                                                                                                                                                              tGGATCACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTACATTCAATAACCTTCGGATTGCCCTGTTTGTCGATCATCAGGCCCCCGTAGAGaa           >  2:563328/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   cACGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGTTTGTCGATCATCAGGCCCGCGTAGAGAAAACCgg     >  1:83014/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     cGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGTTTGTCGATCATCAg                         >  2:364596/1‑229 (MQ=255)
                                                                                                                                                                                                                                                     cGTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGTTTGTCGATCATCAg                         <  1:364596/229‑1 (MQ=255)
                                                                                                                                                                                                                                                      gTCACTGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCTAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGTTTGTCGATCATCAGGCCCGCGTAGAGAAAACCGgtgt  >  1:162795/1‑251 (MQ=255)
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     
AGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGG‑TATGACCCAGCGCGGTGACGCACAGTACGCG‑CCCGCCGTTGGTCACTACCTG‑CTCGTCA‑TCCGCCAGTTTTGTGCCCGCGTGG‑AACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGGACTTCATGCGCAGCATAATCGGCTGGGTTTCCGGATCGCCAAAGCGGCAGTTAAATTCAATAACCTTCGGATTGCCCTGTTTGTCGATCATCAGGCCCGCGTAGAGAAAACCGGTGT  >  NC_000913/4204644‑4205135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑