BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,106,199	A→G	E127G (GAG→GGG)	cpxP →	periplasmic protein CpxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,106,199	0	A	G	99.2%	464.6 / ‑6.1	128	E127G (GAG→GGG)	cpxP	periplasmic protein CpxP
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (65/62); minor base T (0/1); total (65/63)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 128 total aligned reads displayed.

TCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTA  >  NC_000913/4105956‑4106443

tCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGgcagca                                                                                                                                                                                                                                                >  1:750246/1‑250 (MQ=255)

   aCGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAgtg                                                                                                                                                                                                                                            <  2:277518/251‑3 (MQ=255)

   aCGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCg                                                                                                                                                                                                                                            >  1:742633/1‑251 (MQ=255)

       cATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGttt                                                                                                                                                                                                                                        >  2:184524/1‑251 (MQ=255)

                aaCCGACCTTCAGCGCCAGCAGATGCGAGATGTTATGCACAAGGCCCGGCACGA‑CATGCCTCCTGTTACTGTTAGCGAACTGGAGAAAATGCATCGACTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCATGTTAACGCCGGGGCAGCAAGCGGTCTTAAACggg                                                                                                                                                                                                                                >  2:585638/1‑248 (MQ=255)

                 aCCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCTGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACACTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATAGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCATATCAATCGCGTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGaa                                                                                                                                                                                                                              <  1:67705/251‑1 (MQ=255)

                    gAACATCAGCGTCAGCAGATGCGAGATCTTATGCACCAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACCATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAAc                                                                                                                                                                                                                            >  2:926587/1‑250 (MQ=255)

                    gAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAAc                                                                                                                                                                                                                            >  1:703174/1‑250 (MQ=255)

                      aCATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATc                                                                                                                                                                                                                         >  1:225538/1‑251 (MQ=255)

                        aTCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACC‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAACTGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATca                                                                                                                                                                                                                        >  1:473099/1‑250 (MQ=255)

                          cAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACAAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTCAACGAGAAACATcaac                                                                                                                                                                                                                      >  1:919748/1‑250 (MQ=255)

                           aGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATcaaca                                                                                                                                                                                                                     >  1:582730/1‑250 (MQ=255)

                                  gcagATGCGAGATCTTAAGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTGTGCGAACTGGAGACAGTGCATCGCCTTGGCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGGAGAAAAAATGGCGAATGGGCAAATGGCTCTTCAGGTTGAGATGGCAAAAGTCGGCAACCAAATGTATCTCCTTTTAACGCCGGGGGAGCGAGCGGTTTTAGACGAGAAACAGCAACGGCGAATgg                                                                                                                                                                                                             <  2:557671/251‑1 (MQ=255)

                                    tcaTGCGCGATCATATGCACCAGGCCCGGCACGAACG‑GCCTCCTGTCACTGTTAGCGAAGTGGAGACAGTGCATCGCCTTGTCACCGCAGAAAATTTTGATGATAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGGTGAGCTGGCAAAAGTCCGCAAACAAATGAATTGCCTGTTAGCGCCGGGTCAGCAAGCGGCTTTAAACGAGAAACATCAAATACGAATggcg                                                                                                                                                                                                           >  2:584111/3‑249 (MQ=255)

                                    agATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATgg                                                                                                                                                                                                             >  2:78612/1‑249 (MQ=255)

                                    agATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GACTCATGTTAATGTTAGCGAACTGGAGACAATGCATCGCGTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGCAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGAAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATgg                                                                                                                                                                                                             >  1:865760/1‑249 (MQ=255)

                                      aTGCGAGCTCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCaacaac                                                                                                                                                                                                                   >  1:733039/1‑241 (MQ=255)

                                      aTGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCaacaac                                                                                                                                                                                                                   <  2:733039/241‑1 (MQ=255)

                                       tGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGc                                                                                                                                                                                                          >  1:678523/1‑249 (MQ=255)

                                         cGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGtt                                                                                                                                                                                                      <  1:27260/251‑1 (MQ=255)

                                                  aTGCAACAGGCCCGGCACGAACA‑GCCTCCTGGTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGGCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGCATCCCCGGTTAACGCCGGGGCATCAATCGGATTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGcgtgacg                                                                                                                                                                                              <  1:585638/250‑1 (MQ=255)

                                                      aaCAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGtgacgtgacg                                                                                                                                                                                         <  2:703174/251‑1 (MQ=255)

                                                       agaGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGGCACCGCAGAAAATTGTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCTGGTCAGCAAGCGGTTTTAAACGATAAACATCAACAACGAATGGAGCAGTGGCGTTACGGGACGc                                                                                                                                                                                        <  2:436500/249‑1 (MQ=255)

                                                        cAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCa                                                                                                                                                                                       <  2:750246/251‑1 (MQ=255)

                                                          ggCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAAt                                                                                                                                                                                     <  2:675887/251‑1 (MQ=255)

                                                          ggCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAAt                                                                                                                                                                                     <  1:666105/251‑1 (MQ=255)

                                                           gCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATg                                                                                                                                                                                    <  1:97278/251‑1 (MQ=255)

                                                            cccGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGTATGGAGCAGTTGCGTGACGTGACGCAATgg                                                                                                                                                                                   <  1:652036/251‑1 (MQ=255)

                                                               ggCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCa                                                                                                                                                                                 >  2:29912/1‑250 (MQ=255)

                                                                    gAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCaaaaa                                                                                                                                                                             >  2:308249/1‑249 (MQ=255)

                                                                    gAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCaaaaa                                                                                                                                                                             >  1:838529/1‑249 (MQ=255)

                                                                        a‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGCAAcct                                                                                                                                                                                                                          >  2:852448/1‑198 (MQ=255)

                                                                        a‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACAt                                                                                                                                                                                                                          <  1:852448/200‑1 (MQ=255)

                                                                           cctcctGTTAATGTTAGCGAACTGGAGACAATGCAACGCCTTGACACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAAGGGCAAAAAAGTTCATc                                                                                                                                                                     <  2:582730/251‑1 (MQ=255)

                                                                              cctttgaaTGTTAGCGAACTGGAGACAATGCATCGCCGTTTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCGTGTTAACGCCGGGTCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGTATGGAGCAGTTGGGTGACGTGACGCAATGGCAAAAAAGTTCATCg                                                                                                                                                                    <  2:919748/243‑1 (MQ=255)

                                                                              cctGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGtt                                                                                                                                                                  <  1:78612/251‑1 (MQ=255)

                                                                              cctGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGtt                                                                                                                                                                  >  2:106183/1‑251 (MQ=255)

                                                                                 gTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGa                                                                                                                                                                >  1:47922/1‑250 (MQ=255)

                                                                                   tAATGTTAGCGAACTGGAGACAATGCATTGCTGGGTCATCGAAGAAAATTTTGATGAACACGCGGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGAAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAAGGTATCGCCTGTTAACGCCGGGGCAGAAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAg                                                                                                                                                              <  1:712190/250‑1 (MQ=255)

                                                                                       gTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGcgtgacg                                                                                                                                                                                              <  2:255604/214‑1 (MQ=255)

                                                                                       gTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCTTCGTTGAAGCTAtt                                                                                                                                                         >  2:210308/1‑251 (MQ=255)

                                                                                                cTGGAGCCACTGCATCGCCTTGTCACCGCAGAAACTTTTGATGCAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGAACAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGCATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGagtagt                                                                                                                                                  >  2:832321/1‑249 (MQ=255)

                                                                                                 tGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGc                                                                                                                                               >  1:920688/1‑251 (MQ=255)

                                                                                                  ggAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGtagtag                                                                                                                                                <  2:700977/249‑1 (MQ=255)

                                                                                                     gaCAATGCATCTCCTTGTCACCGCAGAAAAGTTAGATGAAAACGCTGTGCGCGCTCAGGCAGAAAAAAGGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTAGCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAACCATCAACAACTAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGGTCATCGTTGAAGCTATTGAGTAGTATCAACt                                                                                                                                           <  2:865760/251‑1 (MQ=255)

                                                                                                           gCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGt                                                                                                                                      <  2:47922/250‑1 (MQ=255)

                                                                                                           gCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAAGGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGGTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGt                                                                                                                                      <  2:920688/250‑1 (MQ=255)

                                                                                                            cATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTc                                                                                                                                    >  1:470036/1‑251 (MQ=255)

                                                                                                             aTCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTcc                                                                                                                                   <  1:410634/251‑1 (MQ=255)

                                                                                                                     gTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAgtagta                                                                                                                           <  1:567821/251‑1 (MQ=255)

                                                                                                                     gTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAgtagta                                                                                                                           <  2:470036/251‑1 (MQ=255)

                                                                                                                        aCCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAAc                                                                                                                        <  1:74329/251‑1 (MQ=255)

                                                                                                                           gCAGAAAATTTTGATGAAAACGCGGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACTAATGGATCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCTGCGTTGAAGCTATTGAGTAGTATCAACTCACGTTCCCAGTAGTAAACCCt                                                                                                                     <  1:403070/251‑1 (MQ=255)

                                                                                                                            cAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTg                                                                                                                    >  2:387233/1‑251 (MQ=255)

                                                                                                                              gAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTaa                                                                                                                          >  2:571357/1‑243 (MQ=255)

                                                                                                                                     ttGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCtt                                                                                                            <  1:590588/250‑1 (MQ=255)

                                                                                                                                          gaAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGGAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCAt                                                                                                       >  2:714507/1‑250 (MQ=255)

                                                                                                                                            aaaCGCTGTGAGCGCACAGGCAGAAAAAATCGCGAACGAGCAATTTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGCGGCAGCAAGCGGTTTTTACCGAGAAACATCAACAACGACTGGCGCGGTTGCGTGACGTGACGCAATGTCAAAAAAGGTCCACGTCGAAGCTATTGAGTAGTTGCAACTCACGTTCCACGTAGTAAACCCTGTTTTCCTTGCCAttg                                                                                                     >  2:613304/1‑248 (MQ=255)

                                                                                                                                             aaCGCGGTGCGCGCACAGGCAGAAAAAATTGCGAATGAGCAAATGGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGGGAAACATCAACAACGAAGGGGGCAGTTGCGTGACGTGACTCAATGGCAGAAAAGTGCATCGTTGAAGCTATTGAGTAGTAGTATCTCACTTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGac                                                                                                   <  1:906543/251‑1 (MQ=255)

                                                                                                                                              aCGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGCATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCagta                                                                                                                              >  2:643709/1‑223 (MQ=255)

                                                                                                                                              aCGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGacc                                                                                                  >  2:114035/1‑250 (MQ=255)

                                                                                                                                              aCGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGaca                                                                                                  <  1:939003/251‑1 (MQ=255)

                                                                                                                                                gCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACAcc                                                                                                >  1:240896/1‑251 (MQ=255)

                                                                                                                                                gCTGTGCGCGCACAGGAAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACAcc                                                                                                >  1:351161/1‑251 (MQ=255)

                                                                                                                                                           acaGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCtt                                                                                     <  1:556694/251‑1 (MQ=255)

                                                                                                                                                             aGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTcc                                                                                   <  1:106183/251‑1 (MQ=255)

                                                                                                                                                               gCAGACAAAATGGCGAATGAGCAACTTGCCCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTCACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGAAGTTGCGTGACGTGACGCACTGGCAAACAAGTTCATCGTTGaa                                                                                                                                                               >  2:788963/1‑173 (MQ=255)

                                                                                                                                                               gCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGTCAAAAAATTTCAGCGTTGaa                                                                                                                                                               <  1:788963/173‑1 (MQ=255)

                                                                                                                                                                cAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTGGCCATAGACGCCATCCCTGTCTTccccc                                                                                <  1:714507/251‑1 (MQ=255)

                                                                                                                                                                       aaTGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCt                                                                         <  1:926587/251‑1 (MQ=255)

                                                                                                                                                                        agggCGAATTAGCAAATTGCGCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCtg                                                                        <  2:542299/249‑1 (MQ=255)

                                                                                                                                                                        aTGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCtg                                                                        <  1:149138/251‑1 (MQ=255)

                                                                                                                                                                          ggCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCtgtg                                                                      <  2:155304/251‑1 (MQ=255)

                                                                                                                                                                            cGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTggg                                                                    >  1:279103/1‑251 (MQ=255)

                                                                                                                                                                            cGAATGAGCAAATTGCTCGTCAGGATGCGATGGCACAAGTCCGCAACCAAATGTATCGACTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGACACATCAACAACGAATGGAGCAGTTGCGTGACGTGAAGCACTGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGAAGCAACTCACGTTCCCAGTAGTACACCCTGTTTTACTTGCCATAGACACCATCACTGTCTTCCCCCACATGCTGgggg                                                                    >  2:824059/1‑251 (MQ=255)

                                                                                                                                                                                tGAGCAACTTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGtt                                                                >  2:374584/1‑251 (MQ=255)

                                                                                                                                                                                 gAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGtt                                                                <  1:172960/250‑1 (MQ=255)

                                                                                                                                                                                       aTTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAAAGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTAGTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCAGAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTa                                                         <  1:832321/251‑1 (MQ=255)

                                                                                                                                                                                         tGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTggg                                                                    >  1:391577/1‑238 (MQ=255)

                                                                                                                                                                                         tGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTggg                                                                    <  2:391577/238‑1 (MQ=255)

                                                                                                                                                                                              gTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCa                                                   >  1:431862/1‑250 (MQ=255)

                                                                                                                                                                                                  ggTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTg                                              >  1:233576/1‑251 (MQ=255)

                                                                                                                                                                                                    ttGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGcc                                            >  1:603998/1‑251 (MQ=255)

                                                                                                                                                                                                       agaTGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCctgc                                         >  1:792407/1‑251 (MQ=255)

                                                                                                                                                                                                       agaTGGCAAAAGTCAGCAAACAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAACCATCAACAACGAAAGGAGCAGTTGCGTGACGAGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTAAATTTGCctgc                                         >  2:635789/1‑251 (MQ=255)

                                                                                                                                                                                                               aaaGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCAt                                                                                                                                                                      <  1:89834/118‑1 (MQ=255)

                                                                                                                                                                                                               aaaGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCAt                                                                                                                                                                      >  2:89834/1‑118 (MQ=255)

                                                                                                                                                                                                                    ccGCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCAtt                            <  2:393221/251‑1 (MQ=255)

                                                                                                                                                                                                                      gCAACCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCAtt                            <  1:887522/249‑1 (MQ=255)

                                                                                                                                                                                                                      gCAACCAAATGTATCGCATGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGTCGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCAtt                            >  2:887522/1‑249 (MQ=255)

                                                                                                                                                                                                                         aCCAAATGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGc                          >  1:494613/1‑248 (MQ=255)

                                                                                                                                                                                                                             aaTGTATCGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTCAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAgatgat                   >  1:340504/1‑251 (MQ=255)

                                                                                                                                                                                                                                    cGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTc            >  2:360465/1‑251 (MQ=255)

                                                                                                                                                                                                                                    cGCCTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTc            <  2:240896/251‑1 (MQ=255)

                                                                                                                                                                                                                                       cTGTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGt         >  1:212218/1‑251 (MQ=255)

                                                                                                                                                                                                                                         gTTAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAACCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCTACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGCTTTGCTTCCGt         <  2:818081/249‑1 (MQ=255)

                                                                                                                                                                                                                                           tAACGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTTCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTtat      <  1:114035/250‑1 (MQ=255)

                                                                                                                                                                                                                                            aaCGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATAc    <  1:29912/251‑1 (MQ=255)

                                                                                                                                                                                                                                            aaCGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATAc    >  1:314751/1‑251 (MQ=255)

                                                                                                                                                                                                                                              cGCCGGGGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTTCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTa  <  1:55795/251‑1 (MQ=255)

TCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA‑GCCTCCTGTTAATGTTAGCGAACTGGAGACAATGCATCGCCTTGTCACCGCAGAAAATTTTGATGAAAACGCTGTGCGCGCACAGGCAGAAAAAATGGCGAATGAGCAAATTGCTCGTCAGGTTGAGATGGCAAAAGTCCGCAACCAAATGTATCGCCTGTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTA  >  NC_000913/4105956‑4106443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`