Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,468,768 C→T W362* (TGG→TGA)  chiA ← endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,468,7680CT98.5% 467.5 / ‑5.4 130W362* (TGG→TGA) chiAendochitinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (73/54);  minor base A (0/2);  total (73/57)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 130 total aligned reads displayed.

CCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCG  >  NC_000913/3468523‑3469012
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
ccccATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGc                                                                                                                                                                                                                                                   <  1:295545/251‑1 (MQ=255)
ccccATGCGTCTTGATAGACAGAACCTTTATAATGTGCATAGTAATACTCTACGATGCTGTCCGCGCTGTACGCTTTGCTAGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTCGTCGGGTTACCGTACTGACTCAATTCCGCAGAGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGGGGGCAATCTTTAGACCCAACCCATCAggg                                                                                                                                                                                                                                                   >  2:495539/1‑250 (MQ=255)
 cccaggctgcTTGATCGAAAGTAGTTTTATAATGTGCATCGTAATAATCTACGATGCGGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGGAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCg                                                                                                                                                                                                                                                  <  2:752896/242‑1 (MQ=255)
   cATGCGTCTGGATCGACAGAAGTTTTAAAATGTGCATTGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGtt                                                                                                                                                                                                                                                <  2:544472/251‑1 (MQ=255)
    aTGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTc                                                                                                                                                                                                                                               >  1:255126/1‑251 (MQ=255)
      gCGTCTTGACCGACAGAAGTTTTATAATTTGCCTCGTTATAACCTACGATGCTTTCCGCGCTCTACCTTTTCCTGGCCTGAAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGTTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCt                                                                                                                                                                                                                                              <  2:782308/250‑1 (MQ=255)
        gTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGt                                                                                                                                                                                                                                           >  2:707970/1‑251 (MQ=255)
         tCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGt                                                                                                                                                                                                                                           >  1:107318/1‑250 (MQ=255)
         gccgagctgACAGAAGTTTGATAAGGTGCCTCGTTAAAATCGACGATGCGGTCCGCGCTGTACGTGTTGCTGGCCTGGAAACCATCCGAAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACGGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGt                                                                                                                                                                                                                                           <  2:33604/242‑1 (MQ=255)
               ccGACAGAGGTTTTATAATGTGCATCGTTATATTCTACGATGCTGTCCGCGCTGTGCGCTTTGCAGGCGGTGAAACCATCCGCAACAATGACGCCTCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTGCCCCGTGGGCAATCGTTAGACCCAACCCATCAGGCGTTCTTGTAAACAtt                                                                                                                                                                                                                                    <  2:104307/250‑1 (MQ=255)
                cGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACAtt                                                                                                                                                                                                                                    >  1:554718/1‑250 (MQ=255)
                 gACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGt                                                                                                                                                                                                                                           >  1:33604/1‑242 (MQ=255)
                 gACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGAGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGAAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTAc                                                                                                                                                                                                                                  >  1:341004/1‑251 (MQ=255)
                  aCAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTAcc                                                                                                                                                                                                                                 >  1:680561/1‑251 (MQ=255)
                      aaGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTg                                                                                                                                                                                                                             <  1:80/251‑1 (MQ=255)
                       aGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGa                                                                                                                                                                                                                            <  1:807244/251‑1 (MQ=255)
                             ataATGGGCATCGTTATAATCTACGATGCTGTCCGCGCTGTCCGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATaa                                                                                                                                                                                                                      <  2:107318/251‑1 (MQ=255)
                                  gtgCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATgg                                                                                                                                                                                                                 >  1:617402/1‑251 (MQ=255)
                                   ggcttCGTTAAAATCGACGATGCTGTCCGGGCTGTCCGTTTGGCGGGCCTGGAAACCATCCGCAACAAGGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGt                                                                                                                                                                                                                <  2:680561/247‑1 (MQ=255)
                                   ggCATCGTTATAATCTACGATGCTGTCCGGGCTGGACGCTTTGCTGGCCGGGAAACCATCCGCAACAAGGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGt                                                                                                                                                                                                                <  2:115254/250‑1 (MQ=255)
                                    gCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTGCCATTGAAAATAACATGGt                                                                                                                                                                                                                <  1:213572/250‑1 (MQ=255)
                                        cGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAtt                                                                                                                                                                                                           >  1:868876/1‑251 (MQ=255)
                                        cGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAtt                                                                                                                                                                                                           >  1:577172/1‑251 (MQ=255)
                                         gTTATAATCTCCGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGCCTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGTGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGCCCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAttt                                                                                                                                                                                                          >  2:614619/1‑251 (MQ=255)
                                         gTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAttt                                                                                                                                                                                                          >  1:223753/1‑251 (MQ=255)
                                         gTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAttt                                                                                                                                                                                                          >  1:531049/1‑251 (MQ=255)
                                            ataATCTACGATGCTGTCCGCGCTGTACTCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTATCCCGGGGGCAATCTTTAGACCCATCCCATCAGGCGTTCTTTTAAACATTATCATTGAAAATAACATGGTAAATTT‑Cg                                                                                                                                                                                                       <  2:434870/250‑1 (MQ=255)
                                            ataATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTGTAGACCCAACCCATCATGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑Cg                                                                                                                                                                                                       <  1:398981/250‑1 (MQ=255)
                                             taATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGCAACTAACCTGGTAAATTT‑ctct                                                                                                                                                                                                     >  2:731816/1‑248 (MQ=255)
                                              aaTCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCt                                                                                                                                                                                                     <  1:170711/250‑1 (MQ=255)
                                              aaTCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCGGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGTCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCt                                                                                                                                                                                                     <  1:854648/250‑1 (MQ=255)
                                               aTCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTAc                                                                                                                                                                                                   >  1:342548/1‑251 (MQ=255)
                                                 cTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCt                                                                                                                                                                                                 >  1:784039/1‑251 (MQ=255)
                                                  tACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCtt                                                                                                                                                                                                                                             <  1:535991/207‑1 (MQ=255)
                                                   aCGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTAc                                                                                                                                                                                                   <  1:870016/247‑1 (MQ=255)
                                                   aCGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCt                                                                                                                                                                                                 <  1:506962/249‑1 (MQ=255)
                                                    cGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCtt                                                                                                                                                                                                >  2:377731/1‑249 (MQ=255)
                                                    cGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCtt                                                                                                                                                                                                <  1:377731/249‑1 (MQ=255)
                                                           gtcctcgcggtccgcgttgtgttccTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGTAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTGTAGACCCAGCCCATCAGGCGTTCTTGTAAACATTGCCATTGAAAATATCATGGTAAATGG‑CGCGACCTGCCTGGcc                                                                                                                                                                                         <  2:576101/226‑1 (MQ=255)
                                                             ccGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTc                                                                                                                                                                                     >  1:663943/1‑251 (MQ=255)
                                                                 gcTGTCCGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTa                                                                                                                                                                                                    <  1:537279/232‑1 (MQ=255)
                                                                 gcTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCCTTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCAcc                                                                                                                                                                                 >  2:792382/1‑250 (MQ=255)
                                                                 gcTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGACGTTATCAATTTCACAGGTAGTCGGGTTACCGAACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGCTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCCGGCGTTCTTGTAAACATTAACATTGAAAATAACATGGTAAATTT‑CGCTaggatcggaagagcgccgtt                                                                                                                                                                                 >  2:537279/1‑232 (MQ=255)
                                                                    gTACGCTTTGCTGGCCTGGAACCCCTCCGCACCAATGACGCCGCCGTTATCAATGTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGGACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCACCCCATCAGGCGTTCTTGTAAACATTACCATTGAAGATAACATGGTAAATTT‑CGCTACCTGCCTGGCCGCTCCACGCtt                                                                                                                                                                              <  2:617402/251‑1 (MQ=255)
                                                                    gTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCtt                                                                                                                                                                              >  1:47775/1‑251 (MQ=255)
                                                                    gTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCt                                                                                                                                                                               <  1:707970/250‑1 (MQ=255)
                                                                           ttGCTGTCCTGGAACCCATCCGCAACAAGGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAAt                                                                                                                                                                         <  2:642103/249‑1 (MQ=255)
                                                                           ttGCTGGCCTGTAACCCATCCGCAAAAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCTTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATg                                                                                                                                                                        <  2:772970/250‑1 (MQ=255)
                                                                           tggCTGGCCGGGAACCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCGTGTAAACAGTACCAGTTAAAATAACATGGTAAATTT‑CGCTACCGTCCTGGCCGCTCCACGGTTGCAATg                                                                                                                                                                        <  2:868876/248‑1 (MQ=255)
                                                                              cTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGt                                                                                                                                                                     >  2:662252/1‑250 (MQ=255)
                                                                                         aCCATCCGAAACAATGACGACGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTAAATTCCGCAGCGGTAGCTGTAAGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCAAACCCATCAGGCGTTCTTGTAAACATTACCCTTGCAAATAACTTGGTAAATTT‑CCCTACCTTCCTGGCCGGTCCACGCTTGCCATGAGTAATCGTTAACGc                                                                                                                                                         >  2:49226/1‑251 (MQ=255)
                                                                                             tCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTATACCCAACCCATCATGCGTGCTTGTa                                                                                                                                                                                                                                          <  1:750701/167‑1 (MQ=255)
                                                                                             tCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTa                                                                                                                                                                                                                                          >  2:750701/1‑167 (MQ=255)
                                                                                              ccGCAACAATTACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACAGTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATcc                                                                                                                                                    <  2:784039/251‑1 (MQ=255)
                                                                                               cGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGTGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CTCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTATCGCTATCCg                                                                                                                                                   <  2:577172/251‑1 (MQ=255)
                                                                                                     aaTGACGCCGCCGTTATCGATTTCACAGGGAGTCGGGTTACGGTATAGACTCACTTCCGCAGCGGTAGCTGTACGCCGGAGACGCCATGGGTTATTGGAGGTTTCAGCGCTGGTGCCCCGTGGGCAATCTTTAGACCCAACCCATCGGGCGTTCTTGTAAACATTATCATTGAAAATAACATGGTAAGTTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATTGTTACCGCTATCCGCTGgtg                                                                                                                                             >  2:830944/1‑251 (MQ=255)
                                                                                                           gccgccGTTATCAATTTCACCGGCAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATg                                                                                                                                       >  1:817872/1‑251 (MQ=255)
                                                                                                                      cAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGtt                                                                                                                             >  2:313160/1‑250 (MQ=255)
                                                                                                                        aTTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACAGTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATTAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATc                                                                                                                          <  1:606695/251‑1 (MQ=255)
                                                                                                                            cacaGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCgg                                                                                                                       <  2:223753/250‑1 (MQ=255)
                                                                                                                             acaGGTAGTCGGGCTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCgg                                                                                                                       >  1:294467/1‑249 (MQ=255)
                                                                                                                                      cGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACTCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCAGTGAAAATAACATGGTAAATTT‑CGCTACCTGCCTGGCCGCTCCACGCTTGCAATGATGAAGCGTTAACGCTAGCCGCTGGGGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTc                                                                                                             <  2:923305/250‑1 (MQ=255)
                                                                                                                                        ggTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCAcc                                                                                                          >  1:13529/1‑251 (MQ=255)
                                                                                                                                          ttACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGATCGCTGTTATCCGGAGTTGGGGTCACCgg                                                                                                        >  1:786200/1‑251 (MQ=255)
                                                                                                                                                 aCTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCTGAGTTGGGGTCACCGG‑TGTTTcc                                                                                                <  1:540174/251‑1 (MQ=255)
                                                                                                                                                 aCTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTcc                                                                                                <  1:195319/251‑1 (MQ=255)
                                                                                                                                                    gACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCg                                                                                               <  2:700637/249‑1 (MQ=255)
                                                                                                                                                             ccGCAGCGGTAGCTGTCCGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCa                                                                                    <  2:231000/251‑1 (MQ=255)
                                                                                                                                                                       aGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCgg                                                                               >  2:141811/1‑246 (MQ=255)
                                                                                                                                                                       aGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCgg                                                                               <  1:141811/246‑1 (MQ=255)
                                                                                                                                                                           gTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTcaca                                                                        <  1:376399/249‑1 (MQ=255)
                                                                                                                                                                               gCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCg                                                                  >  2:660915/1‑251 (MQ=255)
                                                                                                                                                                                    agaCGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCCCCGCCatt                                                             >  2:558867/1‑251 (MQ=255)
                                                                                                                                                                                    agaCGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCatt                                                             >  2:318316/1‑251 (MQ=255)
                                                                                                                                                                                    agaCGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACACCGTGGGCAATCTTTAGACCCAACACATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGACGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCatt                                                             >  1:435039/1‑251 (MQ=255)
                                                                                                                                                                                       cGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTg                                                                                         >  1:92268/1‑220 (MQ=255)
                                                                                                                                                                                       cGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCGTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCGGGGGTTGATGGCTCGCTGGTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTg                                                                                         <  2:92268/220‑1 (MQ=255)
                                                                                                                                                                                        gCCATAGGTTATTGGCGTTTTGAGCGCTAGTATCCGGTGGGCAATTTTTAGACCCACCCCATCAGGCGATCTCGTAAACATTACCATTGAAAATAACATGGCAAATTT‑CGCTCCCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTAGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCACCCGGAGTCACAGCACGCCCattatt                                                          >  2:913088/1‑250 (MQ=255)
                                                                                                                                                                                          cATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATc                                                       >  2:600700/1‑251 (MQ=255)
                                                                                                                                                                                            gggtttATTGGATTTTTCAGCGCTGTTCCCCCGGGGGCAATCTTTAACCCCAACCCAACAGGCGTTCTTATAAACATTACCATTAAAAAAAAC‑TGGAAAATTTTCGCTCCCTCCCGGGCCGCTCCCCGCTTGAAATGAGTAATCTTTACCGCTTTCCGCTTGTGTTGAGGCCTCTCTGTTACCCGTTGTGGGGGTCCCCGG‑TTTGCCCGAAGTGGGATCACCCGGAGTCACAGCACCGCCATTATTATCCg                                                     <  1:898900/247‑1 (MQ=255)
                                                                                                                                                                                                    tGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACg                                             >  1:688997/1‑251 (MQ=255)
                                                                                                                                                                                                    gggAGTTTCCAGCGCGGGTCCCCCGGGGGCAATCTTTAGACCCACCCCATCGGGCGTTTTTTAAAACTTTCCCATTGAAAAAAAAATGGAAATTTT‑CGCTCCCTCCCGGGCCGCCCCCCGCTGGCATGGAGTAAGCGTTAGCGCTAGCCGGTGGTTTTGATGGCTCGGGGTTACCGGGGGTGGGGGTCACGGG‑TGTTTCCGGAGTGGGATCACCGGGAGTCACAGCACCGCCATTATTATCCGGCTTAACg                                             <  1:600700/250‑1 (MQ=255)
                                                                                                                                                                                                          ttgcAGCGCGGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCTCTCCACGCTTGCAATGATTAATCGTTAACGCTATCCGc                                                                                                                                                  <  2:584283/142‑1 (MQ=255)
                                                                                                                                                                                                            tCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAgg                                      >  2:676635/1‑250 (MQ=255)
                                                                                                                                                                                                               gcgcTGGTAACCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGcc                                  >  1:336935/1‑251 (MQ=255)
                                                                                                                                                                                                                cgcTGGTACCCCGGGGGCAATCTTTAGCCCCAACCCAGCGGGCGTTCTGGAAAACATTCCCATTGAAAATAACATGGAAAATTT‑CGCTCCCTTCCTGGCCGCCCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCGGGTGTTGATGGCTCGCTGTTATCCGGGGTGGGGGTCACGGG‑TGTTCCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCa                                 <  1:676635/251‑1 (MQ=255)
                                                                                                                                                                                                                 gcTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGcc                                                                >  2:34765/1‑219 (MQ=255)
                                                                                                                                                                                                                       aCCCCGGGGGCAATCTTTAGACCCAACCCATCCGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGCACAGGAGCCAGGGTTAc                          >  2:368540/1‑251 (MQ=255)
                                                                                                                                                                                                                         cccGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAcc                         >  2:243915/1‑250 (MQ=255)
                                                                                                                                                                                                                          ccttGGGCAATCTTTGGACCAAACCCATCGGGCTTGCTTGAAATCTTTACCATAGAAAAGAAGATGGAAAATTT‑CGCTCCCTTCCTGGCCCCTCGGCGCTTGCTATGATAAATCTTTAACGCTATCCGCTGGTGTTGGTGGCTCGCGTTTACCCGTA‑TGGGGGGCTCGGGGTTTTTCGGGATTGGGATCAACCGGAGTCACACCACCGCTATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCAt                       <  1:495539/248‑1 (MQ=255)
                                                                                                                                                                                                                          ccGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCAt                       >  1:713793/1‑251 (MQ=255)
                                                                                                                                                                                                                           cGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCAt                       >  1:791842/1‑250 (MQ=255)
                                                                                                                                                                                                                            gTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATAc                     >  2:898047/1‑251 (MQ=255)
                                                                                                                                                                                                                             tGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCAc                                                                    >  1:840319/1‑203 (MQ=255)
                                                                                                                                                                                                                               ggCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCCTTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTgg                  >  2:837524/1‑251 (MQ=255)
                                                                                                                                                                                                                               ggCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATTGTAAATTT‑CGCTTCCGTCCGGGCCTGTCCACGCTTGCGATGAGGAATCGt                                                                                                                                                               <  2:886864/111‑1 (MQ=255)
                                                                                                                                                                                                                                   aTCTTTAGCCCCAACCTATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGc                                                                                                                                                  >  2:793708/1‑120 (MQ=255)
                                                                                                                                                                                                                                        tAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGCGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGTTTTCt         >  2:662022/1‑251 (MQ=255)
                                                                                                                                                                                                                                           acccaaaccaTCAGGCGTTCTTGTAAACATTACCATTGCACATAACATGGTCACTTT‑CGCTACCTTACTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACGGGCTGATTTCTgtt      >  2:298989/1‑251 (MQ=255)
                                                                                                                                                                                                                                               aacccaTCAGGCGTTCTTGTAAACATTACCATTGAAAATCACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCAACGCTTGCAATGCGTAATCGTTAACGCTATCGGCTGGTGTTGACGGCTCGCTGTTATCCGGGGTTGGGGTCACCGG‑TGCTTCAGGGGTTGGATCGACCGGAGTCACAGCACCGCCATTATTATCCGGCTTCACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccg  >  1:910823/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
CCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTT‑CGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGG‑TGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCG  >  NC_000913/3468523‑3469012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑