Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,925,117	G→A	P55L (CCG→CTG)	syd ←	SecY‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,925,117	0	G	A	96.0%	252.2 / 1.0	74	P55L (CCG→CTG)	syd	SecY‑interacting protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (35/36);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

GCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTGAAACATGTCTTCT  >  NC_000913/2924872‑2925356                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                gCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTgg                                                                                                                                                                                                                                            >  1:528860/1‑251 (MQ=255)      gcgATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTg                                                                                                                                                                                                                                       <  2:21522/251‑1 (MQ=255)      gcgATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTg                                                                                                                                                                                                                                       >  1:808690/1‑251 (MQ=255)                 ggtgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGTGTTGTATCACAATATCAAAAGCGCTTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACgg                                                                                                                                                                                                                            <  2:57957/250‑1 (MQ=255)                    gggCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCAt                                                                                                                                                                                                                         >  1:655524/1‑251 (MQ=255)                      gcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCt                                                                                                                                                                                                                       <  1:929420/251‑1 (MQ=255)                      gcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCt                                                                                                                                                                                                                       >  1:481611/1‑251 (MQ=255)                       cggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCtt                                                                                                                                                                                                                      <  2:738776/251‑1 (MQ=255)                        ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTc                                                                                                                                                                                                                     >  1:853450/1‑251 (MQ=255)                                tAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGtt                                                                                                                                                                                                              <  2:216820/250‑1 (MQ=255)                                 agtctgttccgAGTCACCAGATGACCAATCAGATTTGCCTGCACCCGACGGAAGTCGGCTTCACTCCAGGTTTGCAGCAATGTCAGGTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTg                                                                                                                                                                                                             <  2:72871/240‑1 (MQ=255)                                 aGCCGTTTCTGAGTCACCAGATGACCAATCAGATTTGCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCCTCTTCGGTAGTTg                                                                                                                                                                                                             <  2:115195/250‑1 (MQ=255)                                                        cccAATCAGATTTTACTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAg                                                                                                                                                                                           <  1:516490/244‑1 (MQ=255)                                                        aCCAATCAGATTTTCCTGCTCCCGACGGACGTCGCCTTCCCTCCAGGGTTTCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAACCTGAGTCGTATAACAGGTATGAATTGTGGGTTATATCACAATATCAAAATCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCTTCTTCGGTAGTTGAACTAATGCACggagacg                                                                                                                                                                                           >  2:516490/1‑240 (MQ=255)                                                          cAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCAATCCAGGTTTGCCGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCg                                                                                                                                                                                   >  1:392095/1‑251 (MQ=255)                                                                  ttttCCTGCACACGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGCGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTc                                                                                                                                                                           >  2:382178/1‑251 (MQ=255)                                                                    ttCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTcc                                                                                                                                                                          <  2:104078/250‑1 (MQ=255)                                                                     tCCTGCACCCGACGGAAGTCGTCGTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCt                                                                                                                                                                         <  2:808690/250‑1 (MQ=255)                                                                           aCCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGGGCATATCCCCGGCAAACTGCGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGAGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTa                                                                                                                                                                   >  2:277393/1‑250 (MQ=255)                                                                                         gtcTTCACTCCAGGTTTGCAGCACTGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCAAGCTTTTAt                                                                                                                                                    >  2:502894/1‑251 (MQ=255)                                                                                         gtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTAt                                                                                                                                                    >  2:147593/1‑251 (MQ=255)                                                                                         gtcTTCACTCCAGGTTCGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGACTTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTAt                                                                                                                                                    >  1:218022/1‑251 (MQ=255)                                                                                             tCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGct                                                                                                                                                 <  1:453480/250‑1 (MQ=255)                                                                                                    aGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAATGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGc                                                                                                                                         <  2:655524/251‑1 (MQ=255)                                                                                                      gTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCGCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCa                                                                                                                                       <  1:338021/251‑1 (MQ=255)                                                                                                            agcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAg                                                                                                                                                                              >  2:303612/1‑206 (MQ=255)                                                                                                            agcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATc                                                                                                                                 <  2:528860/251‑1 (MQ=255)                                                                                                            agcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATc                                                                                                                                 <  1:502894/251‑1 (MQ=255)                                                                                                            agcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGGGAAGGAACACCGTACAg                                                                                                                                                                              <  1:303612/206‑1 (MQ=255)                                                                                                               aaTGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGTGTCGTATAAAAGGTATGAATTGAGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGATGCCAGTATAAGGCATCTTCGGTAGTTGAAATAATGCAAGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCACGCTTTTATGCTCTTCATGCCCCGCATCGc                                                                                                                               >  2:847921/1‑250 (MQ=255)                                                                                                                aTGTCATTTTGATATCGCCAAACGGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGGTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATGTTGGTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTGGAAATAAGGCACGGTGAAGGAACACCGTACATTTCCTCACTTAACGGCCAGCTTTTATGCTGTTCATTCCACGCATCGCAg                                                                                                                             <  2:830939/251‑1 (MQ=255)                                                                                                                   tCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGt                                                                                                                            >  2:289649/1‑249 (MQ=255)                                                                                                                      gTTTGTTATCACCAAACCGCGCGGGCATATCCCCGGCAAACGGAGTCGTATAAAAGGTAGGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACGGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTGGCCAGTATACGGCATCTGCGGTAGTTGAAATAATGCACGGTGAAGGAACCCCGTACAGTGCCGCTCTTAACGGCCAGCTTTTATGCTCTTCATGCCAc                                                                                                                                      <  2:685382/236‑1 (MQ=255)                                                                                                                      gTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGAATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGAAGTTGAAATAATGCACGTTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCAc                                                                                                                                      >  1:685382/1‑236 (MQ=255)                                                                                                                       tttGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCACTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTg                                                                                                                      >  1:866250/1‑251 (MQ=255)                                                                                                                         tGATATCGCCAAACTGCGCGTGAATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGc                                                                                                                     >  1:813581/1‑250 (MQ=255)                                                                                                                              tCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTaa                                                                                                                >  2:318142/1‑250 (MQ=255)                                                                                                                                     aCTGCGCGTGCATATCCACGGCAAACTGAGTCGTATAAAAGGAATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAGTAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTCACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTc                                                                                                        >  1:411826/1‑251 (MQ=255)                                                                                                                                        gcgctttaatatCCCCGGCAAACGGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGGTTACATTTTGTTCCCCGGTAAACAGCTGGGGGTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAg                                                                                                      <  2:99224/242‑1 (MQ=255)                                                                                                                                              gCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTtgt                                                                                               >  2:218712/1‑251 (MQ=255)                                                                                                                                               cATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTtgtg                                                                                              <  1:443957/251‑1 (MQ=255)                                                                                                                                                  atCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCgg                                                                                           >  2:419889/1‑251 (MQ=255)                                                                                                                                                    ccccGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGt                                                                                          <  1:147593/250‑1 (MQ=255)                                                                                                                                                        ggCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAAt                                                                                     >  2:167512/1‑251 (MQ=255)                                                                                                                                                        ggCAAACTGAGTCGTATAAAAGGTATGAAGTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAAt                                                                                     <  2:308946/251‑1 (MQ=255)                                                                                                                                                             aCTGAGTCGTATAAAAGGTATTAATTGTGGGTTGTATCACAATAGCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTGGCCAGGATACGGCATCTTCGGTAGTTGAAAGAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTcc                                                                                <  2:853450/251‑1 (MQ=255)                                                                                                                                                             aCTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTcc                                                                                >  2:187785/1‑251 (MQ=255)                                                                                                                                                                gAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCTTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCGTGGGCGGTCAAATCGTCCACt                                                                             <  1:277393/251‑1 (MQ=255)                                                                                                                                                                  gTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTa                                                                            >  1:107606/1‑250 (MQ=255)                                                                                                                                                                      tataAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCttt                                                                                                         <  2:198689/217‑1 (MQ=255)                                                                                                                                                                      tataAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCttt                                                                                                         >  1:198689/1‑217 (MQ=255)                                                                                                                                                                        taAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTtctc                                                                     >  1:670596/1‑251 (MQ=255)                                                                                                                                                                        taAAAGGTATGAATTGTGGGATGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTtctc                                                                     >  1:303641/1‑251 (MQ=255)                                                                                                                                                                           aaGGTATGAATTGAGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTctctct                                                                  >  1:188204/1‑251 (MQ=255)                                                                                                                                                                            aGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTcc                                                                                >  2:367489/1‑236 (MQ=255)                                                                                                                                                                            aGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTcc                                                                                <  1:367489/236‑1 (MQ=255)                                                                                                                                                                              gTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATc                                                                                                            >  1:577140/1‑206 (MQ=255)                                                                                                                                                                              gTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATc                                                                                                            <  2:577140/206‑1 (MQ=255)                                                                                                                                                                                     ttGTGGGTTGTATCCCATTATCCAAAGAGCGTTCCACCGCGTTTACATTTTGTTCCCCGGTAACCAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGACGGAACACCGTCCAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGc                                                                                                                     >  2:838029/1‑190 (MQ=255)                                                                                                                                                                                     ttGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGc                                                                                                                     <  1:838029/190‑1 (MQ=255)                                                                                                                                                                                      tgtgGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAAc                                                        <  2:421675/250‑1 (MQ=255)                                                                                                                                                                                                aTCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACtt                                             <  2:399891/251‑1 (MQ=255)                                                                                                                                                                                                            aaaGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCATGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACt                                                             <  2:80898/223‑1 (MQ=255)                                                                                                                                                                                                            aaaGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACt                                                             >  1:80898/1‑223 (MQ=255)                                                                                                                                                                                                            aaaGCGCGTACAACCACGTTTACCTTTTGTTCCCCGGTAAACAGCTGGGGCTGACAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTACCTCACTTAAAGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCAATTAAACCATATACACTTGTCgtttagttt                                 >  2:832450/1‑251 (MQ=255)                                                                                                                                                                                                               gcgcGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGGCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGGTTAGTTTATc                              <  2:303641/251‑1 (MQ=255)                                                                                                                                                                                                                          cgcgTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAATGAACACCGTACAGTTCCTCACTTAACGTCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCACGTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGAc                   <  1:934258/251‑1 (MQ=255)                                                                                                                                                                                                                          cgcgTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGAc                   <  1:419889/251‑1 (MQ=255)                                                                                                                                                                                                                           gcgTTTACATTTTGTTCCCCGGGAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCGTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCg                                                                                            <  1:862088/177‑1 (MQ=255)                                                                                                                                                                                                                           gcgTTTACATTTTGTTCAACGGTAAACAGCTGGGGATGCCAGTATACGGCATCTTCGGTAGTTGACATAATGCACGGTGAAGGCACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCCGGGCTTGTGCg                                                                                            >  2:862088/1‑177 (MQ=255)                                                                                                                                                                                                                            cgTTTACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACgg                 >  2:408169/1‑251 (MQ=255)                                                                                                                                                                                                                               ttACATTTTGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACGAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTg               <  1:382178/250‑1 (MQ=255)                                                                                                                                                                                                                                     tttGTTCCCCGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTGAAACAt         <  1:318142/250‑1 (MQ=255)                                                                                                                                                                                                                                           ccccGGTAAACAGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTTGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTGAAACATGtcttct  >  1:79517/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                GCGTCGCGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGCCAGCTTTTATGCTCTTCATGCCACGCATCGCAGTAGCGTGCAGTAAAATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTATCTGGTTTATGACGGTGAAACATGTCTTCT  >  NC_000913/2924872‑2925356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑