Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913766,7160GT33.3% 35.1 / 15.1 40A245S (GCG→TCG) mngA2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base G (15/11);  new base T (13/0);  total (29/11)
Fisher's exact test for biased strand distribution p-value = 6.75e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCAATAGTGCAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTG  >  NC_000913/766495‑766967
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                
ggggAGGCCCGCACCCTTCTGCTTTTTTTCCCACCGCCCCACCTATCGTCTGCGTAACGGCCGGCCGGGGGGGTTTTGCCCCCACCTATATGGCTGCGGAATATCTGAAAAAAGCCGGACAAAAACGCGGCGTAATTTTTTACGTAAAAAAACAAGGCGCTAACGGCATGGAGGGGCTTTTACCGGGGAATCA‑CCTAAATAGCGAAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAg                                                                                                                                                                                                                                    <  1:295728/250‑1 (MQ=255)
     ggCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCAAAAGTGCAA‑ACGCCCGTATTTTTTCGGGTGGAAGT‑GCCATCAAGGGGGGTGAggggtt                                                                                                                                                                                                                               >  1:368147/1‑246 (MQ=255)
        aCGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCC‑ACTCAATAGGGCAA‑CCGCCCGGTTTTTTTTGGGTG‑AAGACGCCATCAAGGagagagaagattggg                                                                                                                                                                                                                             >  1:711572/1‑239 (MQ=255)
                      ctctTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAACA‑ACTCAATAGGGCCA‑CCCCCCGGATTTTTTAGGCTG‑AAGTCCCCACCCAGGGGGGTGAGGG‑GTTTTATGGCATTTCcgcgg                                                                                                                                                                                                             >  1:710837/1‑250 (MQ=255)
                        ctTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACAACTATGTGGGTGCGGAATATCTGGGAAAAGCCGGACGCAAACTCGGCGTAAATGTTTCCGTTGCAAAAAAAGGCGCTAACGGCAGTGAAGGGCGTTTAACGGCGGGTCA‑CATCAATAGGGCAA‑CCGCCTGTCTTTTTGGGGATG‑GAGACGCCATCCAGGGGAGTGGGGG‑GTTTAATGGCATTCCcgcccgg                                                                                                                                                                                                           >  1:910304/1‑247 (MQ=255)
                                                          ggCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑AATCAATACTGGAA‑CCGCCCGTATTTTTTTGGCTG‑GAGTCGCCCTCCAGGGGGGGGAGGG‑GTTTTATTGCATTTCCGCGCCTTCCGTGCCTTGTTgcgcggcggttcgccctgccc                                                                                                                                                                         >  1:287844/1‑232 (MQ=255)
                                                                      gggTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAACC‑AATCCATAATGCAA‑CCGCCTGTGTTTTTTCGGCTG‑AAGTCGCCCTCAAGGGGAGGGAGCG‑GTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGGGGCGATTCGCCCTGCCGAAG‑Cggtggtcc                                                                                                                                                           >  1:815105/1‑244 (MQ=255)
                                                                       ggTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCAATAGTGCAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCCTCAAGGAGAGGGAGCG‑GTTTAATGGCATTCCCCCGCTTTCAGTGCCT‑GTTTCCGAGCCGGTTCGCCATGCAGAAG‑CG‑GTGATccc                                                                                                                                                         >  1:402756/1‑250 (MQ=255)
                                                                         tATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCAATAGTGCAA‑CCGCCCGTATTTTTTCGGCTG‑AAGTCGCCCTCAAGGAGAGGGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GGTGCCGAGCCGCTTCGCCCTGCAGAAG‑CG‑TTGATCcaac                                                                                                                                                       >  1:224033/1‑251 (MQ=255)
                                                                           ttGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑AATCAATAGTGCAA‑CCGCCCGTATTTTTTCGGCTG‑GAGGCGCCACCCAGGAGAGTGGGGG‑GTTTTATTGCATTTCCCCGCTTTCAGGGGCT‑GGTGTCGGGCCGGTTCGccctgacgaagcggtggttccacc                                                                                                                                                        >  2:169248/1‑228 (MQ=255)
                                                                                 acacCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACACCATAGTACAA‑CCGCCTGTATTTTTTCGGCTG‑AAGTCGCCCTCAAGGGGAGTGAGGG‑TTTTAATTGCATTCCCGCGCTTTCAATGCCT‑GTTTCCCAGCCGATTCGCCATGCAGAAG‑CG‑TTTATCCCACAAGCGCtt                                                                                                                                               >  1:682838/1‑251 (MQ=255)
                                                                                 acacCTATACGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCAATAATGTAA‑CCGCCCGTATTTTTTCGGCTG‑AAGTCGCCAACAAGGAGAGGGAGCG‑GTTTTATGGGAGTTCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGGTTCGCCACGCAGAAG‑CG‑GTGATCCAACAAGCGCtt                                                                                                                                               >  1:497708/1‑251 (MQ=255)
                                                                                     cTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCAATAGTGCAA‑CCGCCTGTATTTTTTCGGCTG‑AAATCGCCCTCCAGGAGAGGGGGCG‑TTTTTATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGAATCGCCCTGCAGAAG‑CGCTTG‑TCCCACAAGCCCTTAccc                                                                                                                                           >  1:513618/1‑251 (MQ=255)
                                                                                      tataTGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCAATAGTGCAA‑ACGCCTGTATTTTTGCGGCTG‑AAGTCGCCCTCAAGGAGGGTGAGCG‑GTTTTATGGCATTCCCGCGCTTTTAGGGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTTATCca                                                                                                                                                         >  1:56652/1‑236 (MQ=255)
                                                                                          tGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATAA‑ACTCAAAAGTGCAA‑CCGCCTGTATTTTT‑CGGCTG‑GAGTCGGCATCAAGGAGAGGGGGCG‑GTTTTATGGCAATCCCGCGCTTTTAGTGCCT‑GTTTCCGAGCCGAATCGCCCTGCAGAAGACG‑T‑GATCCAACAAGAGCCTAACCTCAAGc                                                                                                                                     >  1:738532/1‑251 (MQ=255)
                                                                                                                    ggACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCACAC‑CCATAGTGCAA‑CCCCCTGTATTTTTTTGGGTG‑AAGGGGGCCTCAAGGGGAGGGGGGGGTTTTAATG‑CCTTTCCGCGCTTTCAGAGGCC‑GTTGGCGGGCCGCTTCGCCACGCAGAAA‑GG‑TTGGTTCAACAAGCGGTTACCCCCAAGGGGAGCGAGGTGGCGcgtaccagacc                                                                                                            >  2:168497/1‑246 (MQ=255)
                                                                                                                    ggACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCAATAATGCAA‑CCGCCTGTATTTTTTCGGCTG‑AAGTCGCCATCAAGGGGAGTGAGCG‑GTTTAATGGCATTCCCGCGCTTTCAGTGCCC‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGGAGCGGTGAGACGcgtaccgtac                                                                                                             >  1:941707/1‑250 (MQ=255)
                                                                                                                                 gCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCCATAGTGGAA‑CCGCCTGTATTTTTTCGGCTG‑GAGGCGCCATCAAGGAGAGTGAGCG‑TTTTTATGGCATTCCCGCGCTTTCAATGCCT‑GTTGCCGGGCCGCTTCGCCATGCAGAAG‑CG‑GTGATCCAACAAGCGCTTACCCTCCAGCGTAGCGATGAGACGCGTACCGGACAGCAAGATACGcc                                                                                                >  1:313914/1‑249 (MQ=255)
                                                                                                                                   gTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCAATAGTGCAA‑CCGCCCGTATTTTTGTGGCTG‑AAGTCGCCCTCAAGGAGCGTGGGCG‑TTTTTATGGCATTCCCGCGCTTTCCGTGCCT‑GTTGCCGAGCCGATTCGCCCTGCCGAAG‑CG‑TTGATCCC‑CAAGCGCTTACCCCCAAGCGTAGCGATGAGACGCGTACCGTACCGCAAGATACGCAACCg                                                                                            >  1:844822/1‑251 (MQ=255)
                                                                                                                                                                       aTTGAAGGGCGTTTAACGGCGGATCA‑ACTCAATAGTGCAA‑ACGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGGGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCCGAAG‑CG‑TTGATCCAACAAGCGCTTACCCCCAAGCGTAGCGGTGAGACGCGTACCGTACAGCAAGATACGCAACCGGGGAAAAGAGTCAAAAAGGAGCTGAAAAAGGCAcc                                                         >  1:351289/1‑250 (MQ=255)
                                                                                                                                                                              ggCGTTTAACGGCGGATCA‑ACTCAATAGTGCAA‑ACGCCTGTATTTTTGAGGCCG‑AAGTCGCCATTAAGGAGAGCGAGCG‑TTTTAATGGACTTCCCGCGCTTTTAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGGGAAAAGTGTCCAAACGGAGCTGAAACAGGc                                                            >  1:454013/1‑241 (MQ=255)
                                                                                                                                                                               ggctttaaacggcacaccatataaaTAGTGCAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGc                                                  <  1:169248/228‑1 (MQ=255)
                                                                                                                                                                                ggtttaaccggcggtacacaaaaaTAGTGCAC‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAATCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCg                                                 <  1:568455/229‑1 (MQ=255)
                                                                                                                                                                                 ccctgccccggcgcaccaacgcaaaGTGCAAACCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCg                                                 <  1:648226/227‑1 (MQ=255)
                                                                                                                                                                                       cggcggATCA‑ACTCAATAGGGCAA‑CAGCCTGTATTTTTGCGGCTG‑AAGTCGGCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCACCAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAAAAGGCACTGTTGAGCgggataggg                                         >  2:648226/1‑244 (MQ=255)
                                                                                                                                                                                         gggggacaaccaaaatggtgaaa‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCtt                                        <  1:479409/230‑1 (MQ=255)
                                                                                                                                                                                           cgttacacacaaaTAGTACAC‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGTCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCtttt                                      <  1:1144/240‑1 (MQ=255)
                                                                                                                                                                                            gacaaaacaaaagagtacAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTg                                     <  1:150456/234‑1 (MQ=255)
                                                                                                                                                                                            gATCA‑ACTCAATAGTGCAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCACGGAGCGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGc                                    >  2:478818/1‑251 (MQ=255)
                                                                                                                                                                                               cA‑ACTCAATAGTGCAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCC‑AGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGCAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCCCTGTTGAGCGGAATCTCTTTTGCCGtc                                >  2:739172/1‑250 (MQ=255)
                                                                                                                                                                                               aaaatttaatggtgtgagccGCCTGTATTTTTGCGGCTG‑AAGTCGCAATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAAGCGCTTTTGcc                                   <  2:815105/233‑1 (MQ=255)
                                                                                                                                                                                                a‑aCTCAATAGTGCAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGCGTGAGCG‑TTTTCATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGt                                 >  1:480489/1‑250 (MQ=255)
                                                                                                                                                                                                   aaaagaggtccAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCTCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATGCGCCATGCAGAAG‑CG‑GTGATCCAACAAGCGCTTACCCGCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTGTTGCCGTAcc                              <  2:350294/241‑1 (MQ=255)
                                                                                                                                                                                                       agtgtggaacccGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGtt                           <  2:287844/241‑1 (MQ=255)
                                                                                                                                                                                                         aGTGCAC‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTACCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑AG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGCTGAGACGCGTACCGTACAGCACGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGAt                        >  2:757515/1‑251 (MQ=255)
                                                                                                                                                                                                         aGTGCAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGTAGAGGGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCAGCGCAGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCACCCGGTGCAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGAt                        >  1:866138/1‑251 (MQ=255)
                                                                                                                                                                                                            gCAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGt                     >  1:142089/1‑251 (MQ=255)
                                                                                                                                                                                                                   gCCTGTATTTTTGCGGCTG‑AAGTGGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑ATGATCCAACAAGCGCTTCCACTCAAGCGTAGCGTGGAGACGCGTACCGTACAGCAAGATACGCAACCGGGGCAAAGTGTCAAAAAGGCGCTGCAACAGGCACTGTTGAGCGGAATGTCTTTTGCCGTAACGTAGATTGTCGCggg               >  2:541544/1‑251 (MQ=255)
                                                                                                                                                                                                                       gTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCgggggg            <  1:696093/250‑1 (MQ=255)
                                                                                                                                                                                                                         aTTTTTGCGGATG‑AAGTAGCCATCAAGGCGAGTGAGCG‑TTTTAATGGCATTCACGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCCTGCAGAAG‑CA‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAGGAGTCAAAACGGAGCTGAAACAGGCAATGTTAAGCGGACTCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCAc         >  2:417374/1‑251 (MQ=255)
                                                                                                                                                                                                                               gCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCC‑AGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTg  <  1:739172/251‑1 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                
AGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCA‑ACTCAATAGTGCAA‑CCGCCTGTATTTTTGCGGCTG‑AAGTCGCCATCAAGGAGAGTGAGCG‑TTTTAATGGCATTCCCGCGCTTTCAGTGCCT‑GTTGCCGAGCCGATTCGCCATGCAGAAG‑CG‑TTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTG  >  NC_000913/766495‑766967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑