| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 694,721 | C→T | G79S (GGC→AGC) | miaB ← | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 694,721 | 0 | C | T | 98.7% | 248.6 / NA | 75 | G79S (GGC→AGC) | miaB | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (31/43); minor base G (0/1); total (31/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CAATATTTATTGCAGCCTTCCATGATGGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGC > NC_000913/694474‑694963 | cAATATTTATTGCAGCCTTCCATGATGGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGAt < 2:131656/251‑1 (MQ=255) atatTTATTGCAGCCTTCCATGATGGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGCCGCTGATa > 2:472034/1‑250 (MQ=255) atatTTATTGCAGCCTTCCATGATGGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATa > 1:470141/1‑250 (MQ=255) ttattGCAGCCTTCCATGATGGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCTCCGACGCTGATAATCa < 1:454881/250‑1 (MQ=255) attGCAGCCTTCCATGATGGAGACAAACGCGGTCGGCACTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGCAGCTGATATCTACCACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGt > 1:313168/1‑251 (MQ=255) cAGCCTTCCATGATGGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTgg < 1:335302/251‑1 (MQ=255) cAGCCTTCCATGATGGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTgg > 2:416198/1‑251 (MQ=255) tCCATGATGGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAAGCAGGTCTGGAttct < 1:107647/250‑1 (MQ=255) ccATGATGGAGACAAACGCGGTCGGCCCTGCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCGCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGAttcttc < 1:551758/251‑1 (MQ=255) atgaagGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTATACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGTCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTtctc < 2:341530/251‑1 (MQ=255) gatgGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCtt < 1:23664/251‑1 (MQ=255) tgGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGCTGCCACGCAGCCACCGACGCTGATCATCCGGGCTGGATTCTTCTCtttt > 2:170304/1‑251 (MQ=255) gagaAAAACGGGGTCGGCCAGTCGGCGAGCGGTTCCGGTAGACGGTCAAACTGCGCGATTTCCGGGAAGCTGATATCTACAACCGGGATGCGGTCGCCACGAACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCAAAAAATAATATCGACAGAGTGGGCGCGCTGGCGAATGT‑GCTCGCATTAT‑TGCGATGCCACGCAGCCACCGGCGCTGATAATCAGGTCTGGATTCTTCTCTTTTaa < 1:529559/251‑1 (MQ=255) agaCAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCCTCTCTTTTAAc > 2:359327/1‑251 (MQ=255) agaCAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGACGCCACGCACGGAGTTGATCATCTCCGGCAGACGTTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTAGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATACTCAGGTCTGGATTCTTCTCTTTTAAc > 1:235047/1‑251 (MQ=255) aaCGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAGGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGttt > 1:455126/1‑251 (MQ=255) cgcgGTCGGCCCTTCGGCGCGCGGTGCCGGTAGACGGTCAAACTTCTCGATTGCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTcc < 1:28069/251‑1 (MQ=255) cGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGAc < 1:280299/251‑1 (MQ=255) ggCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGACACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGAcc > 2:551045/1‑251 (MQ=255) gCCCTGCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATGTCCGGGAAGCTGATATCTACAACCGGGCTGCTGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCGCTTTTAACAGTTTCCAGCGAccc < 2:29337/251‑1 (MQ=255) cTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGCCATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAAc > 2:217032/1‑251 (MQ=255) tCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTg > 2:605641/1‑251 (MQ=255) cgcgcgGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATgg > 2:526268/1‑251 (MQ=255) cgcgcgGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATgg < 2:470141/251‑1 (MQ=255) ggTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGAc > 1:238145/1‑251 (MQ=255) tCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACtt < 1:342570/250‑1 (MQ=255) cGGTCACACTTCTCGATTTCCGGGAAGCTGATATATACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGGTTGCGGCCCAACAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCCCGCCTTCT‑TGCGATGCCACGCAGCCACCGCCGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGc > 2:571121/1‑251 (MQ=255) gTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCt < 1:449065/251‑1 (MQ=255) aaCTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGCCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTtctc > 1:518570/1‑251 (MQ=255) atgtCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGa < 2:86323/248‑1 (MQ=255) ccGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCCCGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCCTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAg < 1:36590/251‑1 (MQ=255) cGGGAAGCTGATATCTACAACCGGTCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAgg < 1:155778/251‑1 (MQ=255) aaGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCCGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGCCGCTGATAATCAGGTCTGGCTTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGtt > 2:553044/1‑251 (MQ=255) aaGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGtt < 1:448315/251‑1 (MQ=255) aGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTc < 2:135416/251‑1 (MQ=255) atatCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGCGCAGGTGTTcagc > 2:137731/1‑249 (MQ=255) aCAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCagcagcagc < 1:575683/249‑1 (MQ=255) ccGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATcc > 2:174814/1‑251 (MQ=255) cGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGGGCAGCGTCTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGAGTCTTCGCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCACCGCATCCGCttcttc < 2:101059/251‑1 (MQ=255) cGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGc < 2:455126/250‑1 (MQ=255) ttGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTg > 1:156214/1‑251 (MQ=255) tcCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCTGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCgtg > 1:568847/1‑251 (MQ=255) ccGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCgt < 1:295433/249‑1 (MQ=255) ccGGAAGACGGTGCAGCGGTGGCGGCACAAAAATAGGAACGACAAAGTGGGCGCGATGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCCCGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGTAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTAAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCgtgg < 2:432648/251‑1 (MQ=255) ggCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGGCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCgtgggg > 2:449389/1‑250 (MQ=255) aGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGc > 1:437024/1‑251 (MQ=255) gcGGTGCAGCGTTGGCGGCCCAAAAAAAATATCGACTGAGGGGGCGCGCGGGCGAA‑GTCGCTCGCCT‑CTGTGCGATGCCAGGCAGCCACCGACGCGGATAATCAGGTCTGGATCCTGCTCGTCTAACAGGTCCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGCTCAGCAGCAGCGCATCCGTTTCTTCCGCCACGTCGGTCATTTGATTGCCGTGGGCGGCAt < 2:483224/250‑1 (MQ=255) gggTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGGGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATc < 2:192660/250‑1 (MQ=255) gCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCcagc < 1:108378/251‑1 (MQ=255) cGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCcagc > 1:35849/1‑247 (MQ=255) gTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATc < 2:537853/251‑1 (MQ=255) gTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATc < 1:15832/251‑1 (MQ=255) ggCCCAAAAATAATATCTACATAGTGGGCGCTCTGGCGAATGT‑GCTCACCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGTTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGGTTGAGCAGGTGTTCAGCAGCATCACATCCGCTTCTTCCGCCACGTCGGTCAGTTTGTAGCCgtgtgaggcaccgcgccggacggccat > 2:326021/1‑227 (MQ=255) aaaaaTAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCg < 1:472034/251‑1 (MQ=255) aataatATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATg < 1:170304/251‑1 (MQ=255) aataTCGACATGGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCCGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTGGATAGCCGTGGGTGGCATCCAGCAGATCGGCCAGCTTCGATGAAt < 2:316304/251‑1 (MQ=255) aataTCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGCTTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAAt > 1:71777/1‑251 (MQ=255) cGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATTGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGTTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTAc < 1:551045/251‑1 (MQ=255) gACATAGTGGGCGCGCTGGCGAAGGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACt < 2:253330/251‑1 (MQ=255) cATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGCTTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTATCGCGGATTGAGCAGGTGTTCAGAAGCAGCACATCCGCTTCTTCTGCAACGTCGGTCAGTTGATATCCGTGGGTGGCATCTAGCGGATCGGCCATCTTCGCTGAATCGTACTCg > 2:337050/1‑251 (MQ=255) aGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGtt < 1:605641/250‑1 (MQ=255) gTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTc > 1:454519/1‑250 (MQ=255) gCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCCGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGCTGAATCGTACTCGTTCATCTGACAGcccc > 2:482807/1‑251 (MQ=255) gCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGcccc < 1:577576/251‑1 (MQ=255) cGAATGT‑GCTCCCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCATCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCa < 1:279633/251‑1 (MQ=255) gCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCAGCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGt < 1:105772/247‑1 (MQ=255) gCTCGCCTTCT‑TGCGATGCAACGCAGCCACCGAAGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAggc > 2:105772/1‑246 (MQ=255) tGCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAg < 1:174814/248‑1 (MQ=255) gCGATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGTCCCAACTGATGGAAGACTTTTGCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGGGGGTGGCATCCAGCAGATCGTCCATCTTCGATGAATCGTACTCGTTCATCTGACATCCCCAg < 1:408764/233‑1 (MQ=255) gCGATGACACGCAGCCACCGACGCTGATACTCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGCTTGCGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGAGGGTGGACTCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAg > 2:408764/1‑233 (MQ=255) gATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGATCAGGGGTTCAGCAGCAGCACAGCCGCGTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGGTCAGCTGACAGCGCCAGGTTTTAATATGGAGtttttt < 2:53825/251‑1 (MQ=255) gATGCCACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCGTCTTCCGCCACGTCGGTCAGTGGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGtttttt < 1:498486/251‑1 (MQ=255) ccACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATTGAGTTTTTTGGTc < 1:482807/251‑1 (MQ=255) ccACGCAGCCACCGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTc > 1:494228/1‑251 (MQ=255) cccGACGCTGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCCGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTTTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTGCTCGTTCATCTGACTGCCCCAGTTTTTAATATGGAGTTTGTTCGTCATCGACTTGc < 2:348962/250‑1 (MQ=255) | CAATATTTATTGCAGCCTTCCATGATGGAGACAAACGCGGTCGGCCCTTCGGCGCGCGGTTCCGGTAGACGGTCAAACTTCTCGATTTCCGGGAAGCTGATATCTACAACCGGGCTGCGGTCGCCACGCACGGAGTTGATCATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGT‑GCTCGCCTTCT‑TGCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGC > NC_000913/694474‑694963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |