Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,362,045	T→C	intergenic (‑111/+506)	cadC ← / ← pheU	DNA‑binding transcriptional activator CadC/tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,362,045	0	T	C	100.0%	193.7 / NA	53	intergenic (‑111/+506)	cadC/pheU	DNA‑binding transcriptional activator CadC/tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/25);  total (28/25)

AAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTTTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACC  >  NC_000913/4361820‑4362289                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     aaaCCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGATGACGCCAATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGAtt                                                                                                                                                                                                                              >  1:274032/1‑250 (MQ=255)  acccAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTGGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCAACTATAGATGGGTTTATTTACATATTATTTGTGAATGCAAGACGTTATTCGTACCAGCCATAAACTTCTTGATTAc                                                                                                                                                                                                                            <  2:100721/249‑1 (MQ=255)  aaCCAGAAGATCTATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGTTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTAc                                                                                                                                                                                                                            <  2:446145/251‑1 (MQ=255)         agaTCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTAt                                                                                                                                                                                                                     >  1:221208/1‑251 (MQ=255)                   aTCTCGGCTCAAGGGAAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAgg                                                                                                                                                                                                            >  1:481361/1‑250 (MQ=255)                       cggatCAAGGGTAAGTTGACGCCCTTTGCGGCTAATTTGGTTTATGGACGGAGTAAAAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGAtt                                                                                                                                                                                                         <  2:430535/245‑1 (MQ=255)                             aaGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTgag                                                                                                                                                                                                 <  2:199733/251‑1 (MQ=255)                                ggTAAGTTGACGGACATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAAc                                                                                                                                                                                              >  2:55778/1‑251 (MQ=255)                                     gTTGACCCCCATTCCTGCTAATTTGGTTTGTGGACGGATGAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGAAATGATGCAATGATAATTGCCACAACCTATTTTTACCTTCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAGGTCGTTATTCTTACCAGCCATAAACTTCGTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGaa                                                                                                                                                                                         <  2:495591/251‑1 (MQ=255)                                           gCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAat                                                                                                                                                                                    >  2:437463/1‑250 (MQ=255)                                             ccATTGCGGTTATGTTGGTTTATGGACGGAGTAACAA‑ACATTGCGCCAACGGGAACTACAGGTTGTTGCATAATAGAAACCCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTAAATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGGATTACGAAAGGATTTTACTGAGAACCAGAAGTAATAtt                                                                                                                                                                                 <  2:17368/251‑1 (MQ=255)                                                    ggCTAATTTGGTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGACACTCATTCGCAAAGGGAATGATGAACTGATAATTGCCACAACCTATTTTTACCCTCTATAGATGGGTTTATTTCCATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTAcc                                                                                                                                                                          >  1:380157/1‑251 (MQ=255)                                                                  aTGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATtcatc                                                                                                                                                             <  1:470125/250‑1 (MQ=255)                                                                       cGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGAGGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGTTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCAGAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCATAAGTAATATTCCTTACCATCAAAATTCATCATCttt                                                                                                                                                       <  2:286493/251‑1 (MQ=255)                                                                                    cATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAAt                                                                                                                                          >  2:168191/1‑251 (MQ=255)                                                                                     aTT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATg                                                                                                                                         <  2:167319/251‑1 (MQ=255)                                                                                         cGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTc                                                                                                                                      <  1:247797/251‑1 (MQ=255)                                                                                          gCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTc                                                                                                                                      >  2:181209/1‑250 (MQ=255)                                                                                                gcgAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTTCATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAaat                                                                                                                               <  1:258694/251‑1 (MQ=255)                                                                                                 cgAACTACAGGTTGTTGCCTAATAGAAAATCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAACATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAaat                                                                                                                               >  1:495643/1‑250 (MQ=255)                                                                                                   aaCTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAaataat                                                                                                                            >  2:393027/1‑251 (MQ=255)                                                                                                    aCTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATc                                                                                                                           >  1:244598/1‑251 (MQ=255)                                                                                                                    taataGAAACTCATTCGAAAAGGGCATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCg                                                                                                           >  2:450551/1‑251 (MQ=255)                                                                                                                      ataGAAACTCATTCGACAAGGGAATGATGCAATGATCATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAACTTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCgg                                                                                                          >  2:312644/1‑250 (MQ=255)                                                                                                                          aaaCTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGa                                                                                                                                                                                          >  1:493616/1‑166 (MQ=255)                                                                                                                          aaaCTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGa                                                                                                                                                                                          <  2:493616/166‑1 (MQ=255)                                                                                                                                   cGAAAAGGGCATGATGCAATAATACTTGCCACTCCCAATTCTTCCCAACCATAGATGGGATTATTTACCTATAATTGCTGACTGCAACACGTTATTCTTACAAGACATAAACTTCTTGATTACATAGTCTTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCACAATTCCTCATCTTTCCCACAGCAAAATGTTCAGAAAATAATCCATGGAAAATTGCACGGAGCACTTACTCttt                                                                                            >  2:371708/1‑251 (MQ=255)                                                                                                                                         gggAATGATGCAATGATAATTGCCCCAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATgg                                                                                      >  2:595352/1‑251 (MQ=255)                                                                                                                                                atgCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGtt                                                                                >  1:57724/1‑250 (MQ=255)                                                                                                                                                     aTGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGAtt                                                                                   <  1:302145/242‑1 (MQ=255)                                                                                                                                                     aTGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGAtt                                                                                   >  2:302145/1‑242 (MQ=255)                                                                                                                                                        aTAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGc                                                                       <  1:147695/251‑1 (MQ=255)                                                                                                                                                           aTTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAACAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATg                                                                    >  2:220368/1‑251 (MQ=255)                                                                                                                                                                 ccaACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAg                                                              <  1:470256/250‑1 (MQ=255)                                                                                                                                                                    aCCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGa                                                           <  1:246552/251‑1 (MQ=255)                                                                                                                                                                    aCCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGACCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCg                                                            >  1:195445/1‑250 (MQ=255)                                                                                                                                                                           tttACCATCTATAGATGGTTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTTCTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGAt                                                     <  1:55778/250‑1 (MQ=255)                                                                                                                                                                             tACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATAc                                                   >  1:229040/1‑250 (MQ=255)                                                                                                                                                                                cATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACAttt                                               >  1:79885/1‑251 (MQ=255)                                                                                                                                                                                   cTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTtctc                                            >  1:410868/1‑251 (MQ=255)                                                                                                                                                                                     ataGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTtc                                              <  1:560544/247‑1 (MQ=255)                                                                                                                                                                                       aGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCtttt                                        >  2:394674/1‑250 (MQ=255)                                                                                                                                                                                       aGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCttt                                         <  1:505257/250‑1 (MQ=255)                                                                                                                                                                                        gATGGGTTTATTTACATATTATTGGGGAATGCAAGAAGTTATTCTTACCATCCATACACTTCTTGATTACCTAGTATTACGAAAGGATTTTACTGAGAACCAGACGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAGATTGTCCGGAGCACTTACTATTTTACTGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTATCTTTGt                                       >  2:373522/1‑251 (MQ=255)                                                                                                                                                                                                      cATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGGTCAGAAAATAATCCATGGAAAAGTGTCCGGAGCACTTACTATTTTAATGGATTGTTAgcgcttgcat                                                                     <  2:514095/207‑10 (MQ=255)                                                                                                                                                                                                      cATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATAATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCAt                                                                     >  1:514095/1‑207 (MQ=255)                                                                                                                                                                                                         attattGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAAtc                       <  1:437463/250‑1 (MQ=255)                                                                                                                                                                                                           tagtGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGATCTGATGCATTTCTCTTTTTTCGCATTCATAAAAtctc                     <  2:361085/247‑1 (MQ=255)                                                                                                                                                                                                               ggTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtcatca                 <  1:181209/250‑1 (MQ=255)                                                                                                                                                                                                                  gAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATGGTCCGGAGCACTTACTATTTTAATGGATTGTTTGTCGTTGCATGATCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTc              <  1:281650/250‑1 (MQ=255)                                                                                                                                                                                                                   aaTGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGc            >  1:581622/1‑251 (MQ=255)                                                                                                                                                                                                                          aaCGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAg     <  1:338977/250‑1 (MQ=255)                                                                                                                                                                                                                             gTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGAcc  >  1:367605/1‑251 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     AAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATT‑CGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTTTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACC  >  NC_000913/4361820‑4362289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑