Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,241,235	A→G	G340G (GGT→GGC)	xylE ←	D‑xylose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,235	0	A	G	98.5%	223.3 / NA	66	G340G (GGT→GGC)	xylE	D‑xylose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (34/31);  minor base T (0/1);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

GAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGG  >  NC_000913/4240992‑4241478                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    gAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCCGTGGCTTACGGCCAAAtt                                                                                                                                                                                                                                              >  1:581007/1‑251 (MQ=255)           gccagccaCTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGCCAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAACCGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAGATTTATCAACCGt                                                                                                                                                                                                                                    >  2:605959/1‑251 (MQ=255)              agccaCTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAACTTTATCCACCGTCAt                                                                                                                                                                                                                                 >  2:439444/1‑251 (MQ=255)                    tGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAAt                                                                                                                                                                                                                              >  2:246347/1‑248 (MQ=255)                    tGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAAt                                                                                                                                                                                                                              <  1:246347/248‑1 (MQ=255)                     gggCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTg                                                                                                                                                                                                                            <  1:196687/249‑1 (MQ=255)                      ggCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGcc                                                                                                                                                                                                                          >  2:354785/1‑250 (MQ=255)                       gccgccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTCCCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAg                                                                                                                                                                                                                        <  2:345464/251‑1 (MQ=255)                         cgccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGa                                                                                                                                                                                                                       >  1:182504/1‑250 (MQ=255)                          gccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAAc                                                                                                                                                                                                                     <  1:74553/251‑1 (MQ=255)                              ccGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTg                                                                                                                                                                                                                 <  2:32683/251‑1 (MQ=255)                              ccGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTg                                                                                                                                                                                                                 <  2:188793/251‑1 (MQ=255)                                gcgATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGaa                                                                                                                                                                                                               <  1:151561/251‑1 (MQ=255)                                  gATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAgg                                                                                                                                                                                                             <  1:597273/251‑1 (MQ=255)                                      gacAGCGCTTTACCCCGAATAGCATTCGGGAAGATTTCCGCCAGCAGTACCCCGCCTACCGGACCCAAGGACATGGAAAAGGAGGCAACATCGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAACACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATATTTTGCAGTGGCTTACGGCCAAATTTATCCCCCGTCATAATTGCCAGAACGGCGCAGGTg                                                                                                                                                                                                           >  2:215121/3‑249 (MQ=255)                                        cAGCGCTTTACCACGAATCGCATTCGGGAAGCTTTCCGACAGCTGTACCCAGCATACCGGACCCCAGGACATGAAAAAGCCAGCAACCTAGCACAGCACCGCCAGTCGCCCCACATTACCCGCTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACAGATTGCCATTCCGAGTGCGCCGATAAGTTGCAGGGGCTTACGGCCAAATTTATACACCGTCTTACTTGCAAGACCGGTGACGGAGAggg                                                                                                                                                                                                       >  2:532951/1‑250 (MQ=255)                                           cgcTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGa                                                                                                                                                                                                    <  1:32623/251‑1 (MQ=255)                                                tACCACGAATAGCATGCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAAc                                                                                                                                                                                                <  1:439444/250‑1 (MQ=255)                                                 aCCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTc                                                                                                                                                                                              >  1:472669/1‑251 (MQ=255)                                                  ccACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTcc                                                                                                                                                                                             >  2:447565/1‑251 (MQ=255)                                                   cACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGACCAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGCGCGCCGCTCATTTGCAGAGGCTTACGGCCAAATTTCTCCACCGTCATAATTGCCAGAACGGTTAAGGAGAGGTTGATAACtacg                                                                                                                                                                                            >  2:22328/1‑248 (MQ=255)                                                         tAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGACGGTGAGGTTGATAACTCCGACaat                                                                                                                                                                                       >  2:143510/1‑250 (MQ=255)                                                          aGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACaataa                                                                                                                                                                                     <  2:477859/251‑1 (MQ=255)                                                           gCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACt                                                                                                                                                                                               >  2:485172/1‑240 (MQ=255)                                                           gCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACt                                                                                                                                                                                               <  1:485172/240‑1 (MQ=255)                                                           gCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACaataat                                                                                                                                                                                    >  1:588874/1‑251 (MQ=255)                                                                  ggAAGATTTCCGACAGAAGTACCCAGCATACCGGACCCCAGGCCATGGCACAGGCGGCAACATAGAACAGCATCGAAAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGAGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGc                                                                                                                                                                             >  1:525262/1‑251 (MQ=255)                                                                                  cagTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCg                                                                                                                                                             >  1:216003/1‑251 (MQ=255)                                                                                   agTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGt                                                                                                                                                            <  2:144779/251‑1 (MQ=255)                                                                                             aTACCGGACACCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGccccc                                                                                                                                                  >  1:211475/1‑251 (MQ=255)                                                                                               aCCGGACCCCAGGACATCGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACACGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTACACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATCATGGTCAGGAACAGCGCGATATCCGTGCTGGcgccc                                                                                                                                                  >  2:315529/1‑249 (MQ=255)                                                                                                      cccAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTg                                                                                                                                         >  1:579844/1‑251 (MQ=255)                                                                                                       ccAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCAGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTg                                                                                                                                         >  2:420324/1‑250 (MQ=255)                                                                                                         agggcATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAGCAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAac                                                                                                                                      <  1:599393/247‑1 (MQ=255)                                                                                                                   aaaGGCGTCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTTCCGAGGCTAAACATACCTATTGCCTTTCGTAGTGCTCCGATAATTTGCAGTGGCTTAGGTGCAAATTTATCCTGCTGCATAATTGCCAGAACGTTGAAGGTGAGGTTGATAACGCCCACAATAATGGACGGCAACATCGCGATATCCGTGCTGGCTCCCAGCGTTTTGAACACTTCCGgcg                                                                                                                            <  1:293366/251‑1 (MQ=255)                                                                                                                      ggcggcAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGGCTGAGTGTAAAACGCGGTACCGAGGCTAAACAAACCGATTGCCATTCCGAGTGCGCCGAGAATGTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACATGGCGATAGCCGTGCTGGCCCCCAGCGTTTGGAACACTTCCGGCGCgt                                                                                                                         <  2:257288/251‑1 (MQ=255)                                                                                                                                   aaCAGCATCGACAGTAGCGCCACAATACCCGGTGCGTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCTAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCGGAACTGTGAAGGTGAGGTTGATAACTCCGGCAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCACCGTTTTGAACACTTCCGGCGCGTAGTACAGcaccac                                                                                                            <  1:39491/251‑1 (MQ=255)                                                                                                                                           cGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGc                                                                                                    >  1:7644/1‑251 (MQ=255)                                                                                                                                              cAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGCGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGCAGGTGAGGTTGCTAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCACCCAGCGTTTTGCACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCg                                                                                                  >  2:236548/1‑250 (MQ=255)                                                                                                                                               aGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCTGTACCGAGGCTAAAAATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATATCTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTgg                                                                                                                                                       <  1:181614/196‑1 (MQ=255)                                                                                                                                               aGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTTGCCCCCAGCGGTTTGAACACTTCCGGCGCGGAGTACAGCACCACAGTGAGGCCGAc                                                                                                <  2:285453/251‑1 (MQ=255)                                                                                                                                                gtaggcgcacaATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACGCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGGTTTGAACACTTCCGGCGCGTAGTACAGCACCGCATTGATGCCGACa                                                                                               <  2:115832/244‑1 (MQ=255)                                                                                                                                                          aaTACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTgg                                                                                      >  1:203280/1‑250 (MQ=255)                                                                                                                                                              cccGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGa                                                                                     >  1:306508/1‑247 (MQ=255)                                                                                                                                                                cGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATgg                                                                               <  1:475215/251‑1 (MQ=255)                                                                                                                                                                cGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAAGTGCTGGAAGAtag                                                                               <  2:95373/251‑3 (MQ=255)                                                                                                                                                                 ggTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGGTTTGATCACTTCCGGCGCGTAGTACAGCACCCCATTGATGCCGACCAATTGCTGGAAGATgt                                                                               >  2:445934/1‑249 (MQ=255)                                                                                                                                                                         aGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACGCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTa                                                                      <  2:182504/251‑1 (MQ=255)                                                                                                                                                                            gtAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGAGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACAGTCATAATTGCCAGAACGGTGAAGGGGAGGTTGATAACTCCGACACTAATGGTCTGCAACAGCGCGAAATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTCGTACAGCCACACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGc                                                                   >  2:396934/1‑251 (MQ=255)                                                                                                                                                                             aaaaaCGCGGTACCGAGGCTAAACATACCGATGGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCTCCACATGGATGCCGACAAATTGCTGGAAGATGGATAGCATTACGcc                                                                  <  2:550299/250‑1 (MQ=255)                                                                                                                                                                              aaaaCGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCg                                                                 >  2:202529/1‑251 (MQ=255)                                                                                                                                                                                 aCGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTTATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGAtt                                                              <  2:103769/251‑1 (MQ=255)                                                                                                                                                                                          cGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATcacg                                                     >  1:267067/1‑251 (MQ=255)                                                                                                                                                                                                           aTTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATcag                                    <  1:485242/251‑1 (MQ=255)                                                                                                                                                                                                               ccATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATca                                                       >  1:228779/1‑228 (MQ=255)                                                                                                                                                                                                               ccATTCCGAGTGCGCCGATAATTTGCAGGGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGGTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATGGCTGGAAGATGGAGAGCATTTCGCCGATTACAATca                                                       <  2:228779/228‑1 (MQ=255)                                                                                                                                                                                                                cATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAgac                               >  1:288426/1‑251 (MQ=255)                                                                                                                                                                                                                cATTCCGAGTGCGCCGAGAATTTGCGGTGGCTGACGGCCAAATTTATCCCCCGTCAAAATTGCCAGAACGGTGAAGGGGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTCAACGCTGCCGGCGCATAGTACAGCACCACATGGAGTCCTGCAATTTGCTGGAAGACGGAGAGCAGTACGCCGATTACAATCACGCCCACGCCAAACATCTGCAgac                               <  2:546292/251‑1 (MQ=255)                                                                                                                                                                                                                 aTTCCGAGTGCGACGCTCATCTGCAGTGGCTTAAGGCCAAATTTATCAAACGTCATCATTGCCAGAACGGTGCAGGTGAGGTTGATAACTCCGACAATAATGGACTGCAACAGCGCGATATCCGTCCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGCAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAgacg                              >  2:596906/1‑251 (MQ=255)                                                                                                                                                                                                                       ggTGCGCCGATAATTTTCATGGGCTTACGGAGACATTTATCCGCCTTCATAATTGCCAGAACTGTGAAGGTGAGGTTGATAACTCCGGCAAGAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGAAGGAGAGCATTACGCCGAGTACAATCACACCCACGCCAAACATCAGCAGACGaacacc                        <  2:328753/250‑1 (MQ=255)                                                                                                                                                                                                                            gcCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGttt                   >  1:437659/1‑251 (MQ=255)                                                                                                                                                                                                                            gcCGATAATTTGCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATAAGCAGACGACCACCGGttt                   >  2:380734/1‑251 (MQ=255)                                                                                                                                                                                                                                   attggCAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACCGTGACGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCTATATCCGTGCTGGCCCCCAGCGTTTTGAGCACGTGCGGCGCGTAGTACAGCACCTCATTGATTCCGACAAATTGCTGGAAGATGGAGTTCATTACGCCGATTACAATCCCTCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGcc            <  1:349102/247‑1 (MQ=255)                                                                                                                                                                                                                                        cAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGCAGGTGAGGTTGCTAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGAt       >  1:312124/1‑251 (MQ=255)                                                                                                                                                                                                                                        cAGTGGCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACTCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGAt       <  1:58450/251‑1 (MQ=255)                                                                                                                                                                                                                                             gCTTACGGCCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCAGTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAgg  <  1:493748/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGG  >  NC_000913/4240992‑4241478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑