Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,321,967	A→G	intergenic (‑354/+105)	yhbX ← / ← leuU	putative hydrolase YhbX/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,321,967	0	A	G	100.0%	239.8 / NA	67	intergenic (‑354/+105)	yhbX/leuU	putative hydrolase YhbX/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/34);  total (33/34)

TTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGAGCA  >  NC_000913/3321728‑3322209                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   ttttAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTa                                                                                                                                                                                                                                          >  1:104466/1‑250 (MQ=255)  tttAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTaa                                                                                                                                                                                                                                         >  1:469025/1‑250 (MQ=255)         gatgCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTg                                                                                                                                                                                                                                 >  1:421085/1‑251 (MQ=255)         gatgCGGCCATAGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTg                                                                                                                                                                                                                                 >  1:528682/1‑251 (MQ=255)                                 aTTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGCCAAc                                                                                                                                                                                                         >  2:142654/1‑251 (MQ=255)                                      aTAACATTATCAATGCGTCTCTAAACAACACGCAGAGCGTAGCCATCATTCGTTATACTGACAGGAGCAAGAGGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACGCAATAAAGTGCCAGTTATGTCAGTAGTAGGGAAAAAATTATGACTAGGGCGGACCGGCGCCATCGTTTCAAGGTAGCGACTCCGAAGAATGCAACTGGATGAGATACAGGTACCTGACAACCATTc                                                                                                                                                                                                    <  1:375313/251‑1 (MQ=255)                                        aaCATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCt                                                                                                                                                                                                  >  2:397964/1‑251 (MQ=255)                                         aCATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTg                                                                                                                                                                                                              <  2:532072/238‑1 (MQ=255)                                         aCATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTg                                                                                                                                                                                                              >  1:532072/1‑238 (MQ=255)                                            ttATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTc                                                                                                                                                                                                 >  2:23223/1‑248 (MQ=255)                                                cAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGAcc                                                                                                                                                                                            >  2:509548/1‑249 (MQ=255)                                                cAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGAcc                                                                                                                                                                                            >  1:473573/1‑249 (MQ=255)                                                 aaTGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAgg                                                                                                                                                                                         <  2:338334/251‑1 (MQ=255)                                                 aaTGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAgg                                                                                                                                                                                         <  1:8488/251‑1 (MQ=255)                                                 aaTGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAgg                                                                                                                                                                                         >  1:519879/1‑251 (MQ=255)                                                   tGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGAc                                                                                                                                                                                       >  1:603551/1‑251 (MQ=255)                                                        ctctAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCaaa                                                                                                                                                                                   >  2:26299/1‑250 (MQ=255)                                                         tctAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAAc                                                                                                                                                                                 >  1:285985/1‑251 (MQ=255)                                                            aaaCATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGa                                                                                                                                                                               <  2:603551/250‑1 (MQ=255)                                                               cATTAGGCAGAACGAAGCCATCAGTCGTTCTACTGACAGGAGCAGCATGGTTTATCGCGAAGTAAACATATCCGTAATCGTACCGGAACTGGAGTTCACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATCACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGaaaa                                                                                                                                                                            >  2:278854/1‑250 (MQ=255)                                                                    cGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACg                                                                                                                                                                      >  1:423307/1‑251 (MQ=255)                                                                          aCGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                                                <  1:159831/251‑1 (MQ=255)                                                                              aGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGAACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCg                                                                                                                                                            >  2:515676/1‑251 (MQ=255)                                                                                        cGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGTAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATATCTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAATCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTc                                                                                                                                                   <  2:324374/250‑1 (MQ=255)                                                                                                    aGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTAc                                                                                                                                      <  2:119703/251‑1 (MQ=255)                                                                                                    aGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGAGACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACTAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTAc                                                                                                                                      <  1:545743/251‑1 (MQ=255)                                                                                                          aGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAgg                                                                                                                                 >  1:18082/1‑250 (MQ=255)                                                                                                                   tATCGAGAAGTAAACATAGCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCTTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGAGACAGATACCTGACAACCATTCCTCATACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTGGGGACCGAGGACGGGACtt                                                                                                                        <  2:295952/250‑1 (MQ=255)                                                                                                                        agaAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAAccc                                                                                                                  >  1:161602/1‑251 (MQ=255)                                                                                                                          aaGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGt                                                                                                                <  2:519879/251‑1 (MQ=255)                                                                                                                                 cATATCCGTATTCGTACAGGGACTGGCGTTGACACACTCAAGTGCCAATTATGTCAGTAGAAGGGAAAACTTTATACCTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGAAGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGATTTTCAGCGTCTCTTTTCTGGCATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCt                                                                                                         >  2:280437/1‑251 (MQ=255)                                                                                                                                    atCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTACGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTc                                                                                                                                                                                                 >  2:409794/1‑160 (MQ=255)                                                                                                                                    atCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCAGCGTTGCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTc                                                                                                                                                                                                 <  1:409794/160‑1 (MQ=255)                                                                                                                                       cGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTggg                                                                                                   <  2:147279/251‑1 (MQ=255)                                                                                                                                        gTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACg                                                                                                                                                                                <  2:63254/173‑1 (MQ=255)                                                                                                                                        gTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACg                                                                                                                                                                                >  1:63254/1‑173 (MQ=255)                                                                                                                                              gTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCATGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTTGTACCGAGTACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                                                            <  1:240654/251‑1 (MQ=255)                                                                                                                                              gTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTAAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                                                            >  2:579533/1‑251 (MQ=255)                                                                                                                                                 ccGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                                                                         >  1:448486/1‑251 (MQ=255)                                                                                                                                                    gAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTc                                                                                       >  1:194558/1‑250 (MQ=255)                                                                                                                                                      aCTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAg                                                                                    <  1:526227/251‑1 (MQ=255)                                                                                                                                                        tGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAgg                                                                                   >  1:523205/1‑250 (MQ=255)                                                                                                                                                              tGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgt                                                                             <  2:528682/250‑1 (MQ=255)                                                                                                                                                                       aaaGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCaa                                                                    <  1:23223/250‑1 (MQ=255)                                                                                                                                                                        aaGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                                                                  <  2:277020/251‑1 (MQ=255)                                                                                                                                                                         ggTGCCACTTATGTCAGTAGAAGCGCACGCTTCCTAATTAACGCGTACCGGCCACACCGTTTCAAGGTCCCGGCTACGAGTAACGCACCTGGACGAGATACAGATACCTGACAACCATTCCTCAGACTAGGACCACAACGCAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGCAGCGTGTCTACCACTTc                                                                 >  2:296082/2‑251 (MQ=255)                                                                                                                                                                                   aTGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCCTCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGGCGAGATACAGATCCCTGACAACCATTCCCCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGCACCGAGGACGGGAACTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTc                                                        <  2:421085/250‑1 (MQ=255)                                                                                                                                                                                       cAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc                                                   <  2:20912/251‑1 (MQ=255)                                                                                                                                                                                       cAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc                                                   <  1:280088/251‑1 (MQ=255)                                                                                                                                                                                         gTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACgg                                                 <  1:397964/251‑1 (MQ=255)                                                                                                                                                                                           aGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAt                                               <  1:515676/251‑1 (MQ=255)                                                                                                                                                                                           aGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTGCAGCGTCTCGTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCGCCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGa                                                <  1:401734/250‑1 (MQ=255)                                                                                                                                                                                                  aaaaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTTACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATGGGGCACTACCACCTCAATGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACtt                                        <  1:579533/251‑1 (MQ=255)                                                                                                                                                                                                  aaaaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACt                                                                                               <  2:187780/196‑1 (MQ=255)                                                                                                                                                                                                  aaaaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACAGGCTACGAGTAAAGCCACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACt                                                                                               >  1:187780/1‑196 (MQ=255)                                                                                                                                                                                                   aaaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACttt                                       <  2:35883/251‑1 (MQ=255)                                                                                                                                                                                                      aTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACtttttt                                    >  2:205371/1‑250 (MQ=255)                                                                                                                                                                                                        ttATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGt                                  >  1:47905/1‑251 (MQ=255)                                                                                                                                                                                                            aaCTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCgg                              >  1:85160/1‑251 (MQ=255)                                                                                                                                                                                                             aCTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCggg                             >  2:138784/1‑251 (MQ=255)                                                                                                                                                                                                                aaGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGat                           <  2:161602/250‑1 (MQ=255)                                                                                                                                                                                                                aaGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAAACATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGa                            <  1:345418/249‑1 (MQ=255)                                                                                                                                                                                                                  ggCGTACCGGACCCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGTGTGTCTACCAATGCCACCCCCTCGGCACGGATACTTCTTTTTAGTTCGGGATATc                        <  2:395478/251‑1 (MQ=255)                                                                                                                                                                                                                          ggCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCg                <  1:328062/251‑1 (MQ=255)                                                                                                                                                                                                                           gCACCATCGTTTCAAGGTACTGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCACGACCAAAACGAAAACAGACGCTTTTCAGCGTCTCTTTTATGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCCCTACCACCTCAAGGTAGCGTGTCTACAAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGAAATCGCTGGTCgg               >  2:393788/1‑251 (MQ=255)                                                                                                                                                                                                                                  cGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGcc        <  2:194558/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGagca  <  2:448486/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   TTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGAGCA  >  NC_000913/3321728‑3322209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑