Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,216,473	A→G	Y7H (TAC→CAC)	nfeF ←	NADPH‑dependent ferric‑chelate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,216,473	0	A	G	98.9%	261.5 / NA	91	Y7H (TAC→CAC)	nfeF	NADPH‑dependent ferric‑chelate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (31/59);  minor base T (0/1);  total (31/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑AGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTT  >  NC_000913/3216226‑3216712                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                gTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCg                                                                                                                                                                                                                                              <  1:549350/251‑1 (MQ=255) gTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCg                                                                                                                                                                                                                                              >  1:310191/1‑251 (MQ=255)  tataGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCACTCTTCCGATACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGCAGAGTTTGCTGTGATCGGCAAAGCCACGCGATGGAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGGTGCGCTCAACGCGTAACACAGTCAGTTCCCGGAAGAGCAGATCATTGCGAACGCGCTGCGGGT‑GGCgg                                                                                                                                                                                                                                             >  2:555007/1‑251 (MQ=255)   ataGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCgg                                                                                                                                                                                                                                             <  2:98986/250‑1 (MQ=255)      gTCACGCGACGGTGGGCTTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCGCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCgggggg                                                                                                                                                                                                                                         <  1:578542/251‑1 (MQ=255)        cactcgACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCAGTGCGAACGCGCTGTGGGT‑GGCGGGGGgt                                                                                                                                                                                                                                        <  2:214341/246‑1 (MQ=255)          cgcgACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCGGATGCGCTCAACGCGTAACACAGTCAGTTCACGTAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGtt                                                                                                                                                                                                                                     <  2:573481/251‑1 (MQ=255)           gcgACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGtt                                                                                                                                                                                                                                     >  1:488775/1‑250 (MQ=255)            cgACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTAt                                                                                                                                                                                                                                   <  2:188553/251‑1 (MQ=255)              gcGGTGGGCGTGGTCCTTCCGGCCAGACGTTGCCCGCTTCCGTTACCGTTGGCTGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTTAACGCTTAACTCAGTCAGTTCACGGGAGCGCAGTTCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTc                                                                                                                                                                                                                                 <  1:261043/250‑1 (MQ=255)              aCGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTc                                                                                                                                                                                                                                 <  2:133797/251‑1 (MQ=255)                ggTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCAt                                                                                                                                                                                                                               >  1:461536/1‑251 (MQ=255)                  ggggCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGTGGGGTGTTATTCATTg                                                                                                                                                                                                                             <  1:140156/250‑1 (MQ=255)                   gggCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGt                                                                                                                                                                                                                            <  1:7847/251‑1 (MQ=255)                     gCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTa                                                                                                                                                                                                                          <  2:460494/251‑1 (MQ=255)                       gTGGGCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGGGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTGCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGTTGTTATTCATTGTTATc                                                                                                                                                                                                                        <  2:577016/251‑1 (MQ=255)                        tGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGGGTTATTCATTGTTATCg                                                                                                                                                                                                                       >  2:343938/1‑251 (MQ=255)                         ggTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGc                                                                                                                                                                                                                      <  1:458519/251‑1 (MQ=255)                             cTTCCGGCACGACGATGCCCTCTTCCGCTACCGATGGCGGCACCAAGAAAGCGTCAGGTTGAGGAAAGAAGCGTTTGCTGCAATCGTCAAACCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCCATGTTATTGCCTTc                                                                                                                                                                                                                  >  2:591411/1‑251 (MQ=255)                               tCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTc                                                                                                                                                                                                                  <  2:449658/249‑1 (MQ=255)                                ccGGCCCGACGATGCCCTCTTCCGTTACCGGTTGATGCACAAAGTGAGCGTCAGGTTGAGGAAAGACGAGTTTGCTTTGATCGTTAAAGCCACGCGATGTAAAACCGCCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAAGACAGTCAGTTCGCGGAAGCGCAGATCATTGCGGACGCGCTGCGGGT‑TGCGGGTGGTGTTATTCAtctgtatcccctgcgtg                                                                                                                                                                                                               <  2:19509/251‑17 (MQ=255)                                ccGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTg                                                                                                                                                                                                               <  1:26421/251‑1 (MQ=255)                                        aCGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAAt                                                                                                                                                                                                       <  1:228752/251‑1 (MQ=255)                                        aCGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAAt                                                                                                                                                                                                       <  2:585989/251‑1 (MQ=255)                                         cGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATc                                                                                                                                                                                                      <  1:385839/251‑1 (MQ=255)                                          gATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGGAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCATTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGTGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCa                                                                                                                                                                                                     <  1:293608/251‑1 (MQ=255)                                           aTGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAg                                                                                                                                                                                                    <  2:445045/251‑1 (MQ=255)                                           aTGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAg                                                                                                                                                                                                    <  1:555017/251‑1 (MQ=255)                                           aTGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAg                                                                                                                                                                                                    <  2:173750/251‑1 (MQ=255)                                               ccTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGatat                                                                                                                                                                                                <  1:20892/251‑1 (MQ=255)                                               ccTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGatat                                                                                                                                                                                                <  2:44864/251‑1 (MQ=255)                                                    tCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATc                                                                                                                                                                                             <  1:146702/249‑1 (MQ=255)                                                     ccGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCtaa                                                                                                                                                                                          <  1:606576/251‑1 (MQ=255)                                                         tACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGGGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCGCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGGTTTATTCATGGTTATCGCCTTCGGGATGGTAATCAGATATATCtaaata                                                                                                                                                                                       <  2:320053/250‑1 (MQ=255)                                                          aCCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATAGCtaaataa                                                                                                                                                                                      <  1:145076/250‑1 (MQ=255)                                                           ccGTTGGCGGCACAAAGGGAGGGGCAGGTTGAGGAAAGAAGAGTTTGCTGTGAGCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAGACGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGTGTGATATTCATTGTTAGCGCCTTGGTGATGTTAATCAGATATATCTAAATaaaa                                                                                                                                                                                    <  2:247129/251‑1 (MQ=255)                                                              ttGGCGGCACAACGTGAGCGTCAGCTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTc                                                                                                                                                                                 >  2:378443/1‑251 (MQ=255)                                                                              aGCGTCAGTTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGACGCTGAGGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCACAGATCAATGCTAACGCGCTGCTGGT‑GGCGATGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGAtt                                                                                                                                                                 <  1:405652/251‑1 (MQ=255)                                                                                 gTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGtt                                                                                                                                                              >  2:229457/1‑251 (MQ=255)                                                                                 gTCAGGTGGAGGAAAGAAGAGTTTGCTGGGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCGGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGGTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGGGTGATTCATTGTTAGCTCCGGCGTGATGGTGATCGGATATATCTAAATAAAACTCGCAAACGAGAATGATTGtt                                                                                                                                                              <  2:535030/251‑1 (MQ=255)                                                                                      gTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTACAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATc                                                                                                                                                          >  1:367172/1‑250 (MQ=255)                                                                                        tGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATACAACTCGCAAATGATAATGATTGTTAATCATg                                                                                                                                                       >  2:233804/1‑251 (MQ=255)                                                                                         gAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGa                                                                                                                                                      <  1:431057/251‑1 (MQ=255)                                                                                           ggAAAGAAGAGGTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAAGAAAACGCGCAAATGATAATGATTGTTAATCATGATa                                                                                                                                                    <  2:81574/251‑1 (MQ=255)                                                                                            gAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATa                                                                                                                                                    >  1:497660/1‑250 (MQ=255)                                                                                                gaagaGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGGCCAGCGCCTCGCCGCCGAGGACAATGCGCGGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCGGCGGGT‑GGTGGGGGGTGGTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAAGGATAATTATTGTTAGTCATGATAAATGc                                                                                                                                               <  2:585011/251‑1 (MQ=255)                                                                                                  agagTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCaa                                                                                                                                             <  1:343938/251‑1 (MQ=255)                                                                                                  agacgttGCTTTGAGCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCGCGCAGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCATATCGTTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCTTGGTTATCGCCTTCGGGATGGTTATCAGAGATAACAAAATAAAACTCGCTAAAGATAAGGATTGtgagttatgattaatgaaa                                                                                                                                             <  2:367237/246‑19 (MQ=255)                                                                                                   gagTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAg                                                                                                                                            <  1:425557/251‑1 (MQ=255)                                                                                                               aTCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGaa                                                                                                                                <  1:219665/251‑1 (MQ=255)                                                                                                                 cGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAAGACACTACGCTGAAAACCGGCGCTGATGAGATCACCGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGCTATTCATTGTTATCGCCCTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACt                                                                                                                              >  1:283454/1‑251 (MQ=255)                                                                                                                  gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTg                                                                                                                             >  1:341494/1‑251 (MQ=255)                                                                                                                   tCAAAGCCACGCGATGAAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGAGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCACATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTg                                                                                                                             >  1:610657/1‑250 (MQ=255)                                                                                                                       aGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGCGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACCGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATg                                                                                                                        >  1:253715/1‑251 (MQ=255)                                                                                                                       aGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATg                                                                                                                        <  1:427447/251‑1 (MQ=255)                                                                                                                           aCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCt                                                                                                                     >  1:512536/1‑250 (MQ=255)                                                                                                                           aCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGGGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTca                                                                                                                     >  2:82352/1‑249 (MQ=255)                                                                                                                             gcgATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCtata                                                                                                                  <  1:142113/251‑1 (MQ=255)                                                                                                                               gATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGt                                                                                                                >  2:373904/1‑251 (MQ=255)                                                                                                                               gATGTAAAACCGTCCAGCGCCGCGCCGACGAGGACAAGGCGCTGAAAATCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCTCGGAAGAGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATACATTGTTATCGCCTTCGTTATGGTAATTAGATATAGCTAAATAAAACTCGCAAATGATAATGGTTGTTAATCATGATAAATGCAAGCGATGTGTAGAACGGATATGTCTATAGt                                                                                                                <  1:372798/251‑1 (MQ=255)                                                                                                                                     aaaCCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATa                                                                                                          <  1:98798/251‑1 (MQ=255)                                                                                                                                      aaCCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATaa                                                                                                         <  2:196921/251‑1 (MQ=255)                                                                                                                                         cGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGAc                                                                                                      <  1:330671/251‑1 (MQ=255)                                                                                                                                          gTCCAGCGCCTCGACGGCGTGGACGATGCGCTGCAAACCGGCGCTGATGCGCCCAACACGTAACACAGTCACATCCCGGAGGCGAAGATCATTGCGCACGCGCTGCGGGT‑GGCGGGGGGGGTTATTCAATGGTATCGACTTCGAGATGGTAATACGATATCTCTCAATAAACATCGCGAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAAAACTGATATGTCTATAGTATGATAAGACg                                                                                                     >  2:307452/1‑251 (MQ=255)                                                                                                                                              aGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGCCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGAGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAAcc                                                                                                 >  1:335146/1‑251 (MQ=255)                                                                                                                                                cgcCTCGCCGCCGAGGACACTGCGCTGAAAAACGGCGCTGCTGCGCTCCACGAATAACACAGTCCGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGATATTCATTGTTATCGCCTTCGCGATGGTAATCAGATATATCTAACTAAACCTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATACTCTGATAAGACGAACCGc                                                                                               >  2:599526/1‑251 (MQ=255)                                                                                                                                                 gcCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGCTATATCTAAATAAAACTCGCAAATGATAATGCTTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGcc                                                                                              >  2:87179/1‑251 (MQ=255)                                                                                                                                                     cgccgcCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCctc                                                                                            <  1:338804/249‑1 (MQ=255)                                                                                                                                                     cgccgcCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCtctt                                                                                          <  2:35039/251‑1 (MQ=255)                                                                                                                                                     cgccgcCAAGGACAATGCGCTGAAAACCGGCGCTGCTGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGAGCAGCTCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGATATTCCTTGTTATCGCCTTCGTGATGGTCATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGCTTTGTAGAACTGATCTGTTTATAGTCTGATAAGACGAACCGCCtctt                                                                                          >  2:516282/1‑251 (MQ=255)                                                                                                                                                          ccgAGGACAATGCGCGGAAACCCGGGGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCTGTGCGAACGCGCTGGGGGT‑GGCGGGGGGTTTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAAAAAAACTCGCAAATGATAATGATTTTTAATAATGATAAATGCAGGCGATTTGTAGAACGGATAGGTTTATAGTCTGATAAAACGATCCGCCTCTTCTCAg                                                                                     <  2:73145/251‑1 (MQ=255)                                                                                                                                                                        cTGAAAACCGGCGGTGATGCGCTCAACGCGTAACCCAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCAGCGGGT‑GGCGGGGGGGGATATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCCAAATAAAACTCGCAAACGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACAGATATGTCTATAGTCTGATAAGACGACCCTACTCTTCTCAAGCATCGTTAccca                                                                        >  2:472464/1‑247 (MQ=255)                                                                                                                                                                          gAAACCCGGCGCTGAGGCGCTCAACGCGTAACACAGTCATTTCAGGGAAGCGCAGATCATTGCGAACGCGCTGCGGGG‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTTTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAAc                                                                      <  1:412525/250‑1 (MQ=255)                                                                                                                                                                            aaaCCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCAt                                                                                                                                                        <  2:43774/166‑1 (MQ=255)                                                                                                                                                                            aaaCCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCAt                                                                                                                                                        >  1:43774/1‑166 (MQ=255)                                                                                                                                                                             aaCCGGCGCTGATGCGCTCAACGCGGAACACAGTCAGTTCACGAAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGGGTTATTCATTGTTATCGCCTTCGCGCTGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCg                                                                  >  1:310753/1‑251 (MQ=255)                                                                                                                                                                               ccGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCgg                                                                 >  1:148065/1‑250 (MQ=255)                                                                                                                                                                                       gATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGt                                                        >  1:14894/1‑251 (MQ=255)                                                                                                                                                                                                   gcgTAACACAGTCAGTTCACGGAAGCGCAGTGCATTGCGTACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCAGTGTTAGCGCCTTCCTGATGGTAATCAGat                                                                                                                                                                                                  <  2:236942/101‑1 (MQ=255)                                                                                                                                                                                                   gcgTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTAATTGTTATCGCCTTCGTGATGGTAATCAGat                                                                                                                                                                                                  >  1:236942/1‑101 (MQ=255)                                                                                                                                                                                                    ggTAACACGGGCAGTTAAGGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGGGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAAGGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGAAAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATcc                                           <  2:488775/250‑1 (MQ=255)                                                                                                                                                                                                          acaGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCCGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCGGATATATCTAAATAAAACTCGAAAATGATAAGGATTGTTAATCATGATAATTGCAAGCGATTTGTAGAACTGATAGGTCTATGGTCTGATAAGATGTACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                     <  1:317570/251‑1 (MQ=255)                                                                                                                                                                                                           cagtcagtTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGG‑GGCGGGGGGTGTTATTCATTTTTATCGCCTTCGGGATGGAAATCAGATATATCAAAAAAAACCTCGCAAATGATAATGATTGTTAATCAATATAAATGCAAGCGATGTGTAGACCGGTTATGTCTAGAGTCGGATAAGACGAACCGCCTCTTCTCAGTCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                    <  1:378443/251‑1 (MQ=255)                                                                                                                                                                                                               catttCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑GGGGGGGGGTGTTATTCATTGTTAGCGCTGTCGTGATGGTAATCAGATATATCTAAAAAAAACTGGCAAATGATAAGGATTGTTAATCGTGATAAATGCAAGCGATTGGTAGAACTGATGGTTTTATAGTCTGATAAGAGGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACgtgt                                <  1:273421/248‑1 (MQ=255)                                                                                                                                                                                                                 ggtcccggaagcgcgaatatttGCGAACGCGTTGGGGGT‑GGCGGGGGGTTTTATTCATTGTTCTCGCCTTCGTGATGGTAATCAGATATATCTAAAAAAAACTCGAAAATGAAAAGGATTGTAAATCATGAAAAATGCAAGCTATTTGTAGAACGGATATGTTTATAGTCGGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTga                              <  2:148065/231‑1 (MQ=255)                                                                                                                                                                                                                             gcAGATCGTTGCGACCGCGCT‑CGGGTTGGCGGGGGTTGTTTTTCATTGTTACCGCTTTCGTGTTGTTAATCAGATATATCTAAAAAAACCTCGCAAATTATAATTATTGTTAACCATGATAAAGGCAAGAGTTTTGTAGAACGGATATGTCATTAGTCTGATAAGTCGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCTGCCTGCGTGTGAGATGAGTCCACg                  <  2:610657/251‑1 (MQ=255)                                                                                                                                                                                                                              cAGATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACCCGCAAATGATAATGATTGTTAATCATGATAAATCCAAGCGTTTTGTAGAACTGATATTTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGt                 <  1:237592/251‑1 (MQ=255)                                                                                                                                                                                                                                gATCATTGCGAACGCGCTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTAAAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGttt               <  1:233804/251‑1 (MQ=255)                                                                                                                                                                                                                                 aTCATTGCGACCGCGCTGCGGGG‑GGGGGGGGGTGTTATTCATTGTTATCGCTTTCGTGATGGAAATAAGATATATATAAATAAAACTCGAAAATGAAAATGATTGTTAATCATGAAAAAGGAAAGCGATTTGTAGACCTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTa              <  2:158424/251‑1 (MQ=255)                                                                                                                                                                                                                                             gcgcTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCCAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGAATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGCGTGAGATGAGTCCCCGTTTAAAAATCAttttt  >  2:429303/1‑251 (MQ=255)                                                                                                                                                                                                                                               gcTGCGGGT‑GGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGtgtg                               >  1:11576/1‑220 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                GTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGT‑AGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTT  >  NC_000913/3216226‑3216712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑