Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,214,325	T→C	F427L (TTC→CTC)	rpoD →	RNA polymerase sigma factor RpoD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,214,325	0	T	C	100.0%	315.9 / NA	95	F427L (TTC→CTC)	rpoD	RNA polymerase sigma factor RpoD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (55/40);  total (55/40)

AGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAA  >  NC_000913/3214080‑3214570                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      agcagATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTcc                                                                                                                                                                                                                                                  >  2:459067/1‑251 (MQ=255)  gcagATTGAAGAAGAACCCGGCCTGACCATCGAGCAGGTTAAAGATATCACCCGTCGTATGTCCATCGGTGAAGCAAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCa                                                                                                                                                                                                                                                 <  1:305973/251‑1 (MQ=255)        taaagaagaaCCCGGCCGTACCAACGAGCGGTTTAAAGATATCAACCTTGGTATGCCCATCGGTGAAGCGAAGGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACTTCTGGTTATTTCGATCGCTAAGAAATACACCAACCGGGGCTTGCAGTTCCTGGACCTGAGTCAGGAAGGCAACAT‑CGTTCTGATGAAGGCGGTTGATAAATTCGAAAACCGCCGTGGTTACAAGCTCTCCACCTAc                                                                                                                                                                                                                                            <  2:498101/248‑1 (MQ=255)         gaagaagcaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGCAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGc                                                                                                                                                                                                                                          >  1:451041/1‑251 (MQ=255)             aagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAAcc                                                                                                                                                                                                                                      <  1:429105/251‑1 (MQ=255)             aagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAAcc                                                                                                                                                                                                                                      >  2:293531/1‑251 (MQ=255)                   ccGGCTTGACCATCGAGCAGGTTAAAGATATCACCCGTCGTATTGCCATGGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCTACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCGCCTACGCAACTtggtgg                                                                                                                                                                                                                                <  1:539057/251‑1 (MQ=255)                   ccGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCtggtgg                                                                                                                                                                                                                                >  1:500485/1‑251 (MQ=255)                     ggCCTGACCATAGAGCAGGTTAAAGATATCAACCGTCGTCTGTCCATCGGAGAAGCGAAAGCCCGCCGTGCGAAGCAAGAGATGGTTGAAGAGAACTTCCGTCTGGTTATTTCTCTCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCCACTTGGTGGAt                                                                                                                                                                                                                              >  2:512948/1‑251 (MQ=255)                        cTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCCCCTCCGCCACCTGGTGGATCCg                                                                                                                                                                                                                           >  2:482559/1‑251 (MQ=255)                          gACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTc                                                                                                                                                                                                                         >  2:344257/1‑251 (MQ=255)                          gACCATCAAGCAGGCAAAAGATATCAACCGCCGAATGTCCATCAGTGACGAGAAAGCCCGCAGTGCGAAGAAAGAGATGGTTGCAGCGAACTTAAGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGCTCCTTGACCTGACTCAGGAAGGCAACAT‑CGGTCTGATGACAGCGGGTGATAACTTCGAATACAGACGTGGTTACAAGCTATCAACCTACGCAACTTGGTGGAGCCGTc                                                                                                                                                                                                                         >  2:254329/1‑251 (MQ=255)                           aCCATCGAGCAGGTTAAAGATACAAACCGGCGTATGTCCATGGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTTACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCa                                                                                                                                                                                                                        <  2:412079/251‑1 (MQ=255)                                 gggCAGGTTAAAGATATCACCCGTCGTATGTCCATCGTTGAAGCGAATCCCGGCCGTGCGAAGAAAGAGAGGGTTGAAGCGAACTTACGTCTGGTTATTTCTTTCGCAAAGAAATACACCAACCGTGTCTTGCAGTTGTTTGTCCTTTTTCAGGAAGGCAACAT‑CGGTCTGTTGAAAGCGGTGGATAAATTCGAATACCGCCGGGGGTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGAt                                                                                                                                                                                                                  <  1:597025/249‑1 (MQ=255)                                   gCAGGTTAAAGCTATCCACCCTCGTACGTCCCTCGGTGAAGCGCAAGCCCGCCGCGCGCAGAAAGAGATGGATGAAGCGAACTTACGTCTGGATATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGCTCCGTCAGGCGATcc                                                                                                                                                                                                                >  2:375630/1‑250 (MQ=255)                                       gtaaaaGATACCAACCGTCGTATGTCCACCGGTGAATCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAATCGAACTTACGTCTGGTTATTTCTATCGCTAAGAATTACACCAACCGTGGCTTGCAGTTCCTTGTCCTGATTCAGGAAGGCAACAT‑CGGTCGGATGAAAGGGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCGCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCg                                                                                                                                                                                                            <  1:229812/248‑1 (MQ=255)                                       gTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAACCCGACGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGCAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGCCAACAT‑CGGTATGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGCTAACCCg                                                                                                                                                                                                            >  1:554383/1‑251 (MQ=255)                                         tAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGct                                                                                                                                                                                                          >  1:191920/1‑251 (MQ=255)                                             gATATAAACCGTAGAATGTCCATCGGTGGAGCGAAAGCCCGCAGTGCGAAGAAAGAGATGGTTGAAGCGCACTTACGTCTGGTTATTTCTATAGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGTAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGAGGAGCCGTCAGGCGCTCCCCCGATCTAt                                                                                                                                                                                                      >  2:563265/1‑251 (MQ=255)                                                     ccGTGGTATGTCCCTCGGTGAAGCAAAAGCCCGCCGGGCGAAGAAAGAGAGTGTGGAAGCCAACTTTCGTCGGGTTTTTTCTATCGTTAAAAAATACACCAACCGTGTCGTGCAGTTCCTTTACCTGATTCAGGAAGGCCACTT‑CGGTCTGATGAAAGCGGTGTATAGATGCGAATACCGCCGTGGTTACAAGCTCTCCACCTTCGCAACCGGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATc                                                                                                                                                                                              <  2:361552/251‑1 (MQ=255)                                                     ccGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATc                                                                                                                                                                                              >  1:236611/1‑251 (MQ=255)                                                       gtcgtATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATc                                                                                                                                                                                              >  1:251363/1‑249 (MQ=255)                                                            aTGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGATCTTACGTCTTGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGTGCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCGCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGgcgcg                                                                                                                                                                                       <  1:531441/251‑1 (MQ=255)                                                            aTGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGgcgcg                                                                                                                                                                                       >  2:132003/1‑251 (MQ=255)                                                            aTGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGgcgcg                                                                                                                                                                                       >  1:392093/1‑251 (MQ=255)                                                             tgcccagcGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGAGGTTTGATGCGAACTTACGTCGTGTTATTTTTACCGCTAAGAAATACACAAACTGTGGCTTGCATTTCCTTGACCTTAGTCAGGAAGGCAACAT‑CGGTCTGTTGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCTCCTACGCAACCTGGTGGATCCGTCAGTCGATCACCCGTTCTATCGCGGATCAGgcgcg                                                                                                                                                                                       <  2:389660/243‑1 (MQ=255)                                                                ccATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTACGAAATACACCAACCGTGGCTTGCAGTTACTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCAcc                                                                                                                                                                                   >  1:406482/1‑251 (MQ=255)                                                                    ggggaaaGCAAAAGCCCGCCGTGCGAAAAAAGAGATGGATGAAGCAAACTGACGTCGGGTTATTTCTATCGCTAAGAAAAACACCAGCCGGGGCTTGCAGTTCCTTGACCTGATTCAGGAAGTCAACAT‑CGTTCTGATGAAATCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATcc                                                                                                                                                                               <  2:392093/246‑1 (MQ=255)                                                                    gggTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATcc                                                                                                                                                                               <  2:191920/250‑1 (MQ=255)                                                                         aaGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTAtt                                                                                                                                                                          <  1:344257/251‑1 (MQ=255)                                                                          aGCGAAAGCCAGCAGTGCAAAGAAAGATATGGTTGAACAGAACTCCCGTCGGGTACTTAATATCTCAACGAAATCCACCAACGGGGGCTTGCAGTCCCTGGACCTTATTACGGAAGGCACCAT‑CGGGCTAATGAAAGCGGTTGAAAAATTCGAAGTCCGCCGTGGTTACAAGCTCTCCACCTACGCAACCGGGTGGATCCGTCAGGCGATCTCCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTc                                                                                                                                                                         <  2:251294/251‑1 (MQ=255)                                                                                   ccgccgTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGACAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCat                                                                                                                                                                 >  1:374419/1‑250 (MQ=255)                                                                                     gccgTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATg                                                                                                                                                              >  2:376948/1‑251 (MQ=255)                                                                                        gTGCGAAGAAAGAGATGGTTGACGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGAtt                                                                                                                                                           >  1:468322/1‑251 (MQ=255)                                                                                         ggCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCACCCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCAGATGATTg                                                                                                                                                          <  1:154344/250‑1 (MQ=255)                                                                                            gaagaaAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTg                                                                                                                                                          >  1:454162/1‑248 (MQ=255)                                                                                              agaaagaGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGAcc                                                                                                                                                     >  1:32615/1‑251 (MQ=255)                                                                                                 aagaGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATc                                                                                                                                                                                                                 <  1:217512/188‑1 (MQ=255)                                                                                                 aagaGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATc                                                                                                                                                                                                                 >  2:217512/1‑188 (MQ=255)                                                                                                   gagaTGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTGGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATCCCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATcaa                                                                                                                                                <  1:310048/251‑1 (MQ=255)                                                                                                     gaTGGTTGAAGAGAACTTACGTCTGGTTATTTCTATCGCAAAGAACTACACCAACAGTAGCTTGCAGTTACTTGACCTGATTCAGGAAGGCAACCT‑CGGTCTGATGAAAGCGGATGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACATACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCCGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATcaac                                                                                                                                               >  2:549015/1‑250 (MQ=255)                                                                                                     aatggtttaaggaaaCTTACTTCTGGTTATTTCAACCGCAAAGAAATACACCAACCGTGCCTTTCAGTTCCTGAACCTAATTCAGGAAGACACCAT‑CGGTCGGATGAAACCGTTTAATAAATCCAAATACCGCCGTGGTTACATGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCTATCACCCGCTCTAACGCGGATCAGGCGCGCACCATCCGTATTCCGTTGCATATGATTGAGACCATcaaca                                                                                                                                              <  2:391911/238‑1 (MQ=255)                                                                                                        ggTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTCAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGACTACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGCTCAGGCGGGCACCATCCGTATTCCGGTGCTTATGATTGAGACCATCAACAAGc                                                                                                                                           >  2:102680/1‑251 (MQ=255)                                                                                                           tGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGAAGTGCCTTGACCTGATTCAGGAAGCCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTc                                                                                                                                         <  2:155885/250‑1 (MQ=255)                                                                                                            gAAGCGACCTTACGTCTGGTTATTTCTATCTCAAAGAAATACCCCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGTTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCaa                                                                                                                                       <  2:454162/251‑1 (MQ=255)                                                                                                             aaGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAAc                                                                                                                                      >  2:351188/1‑251 (MQ=255)                                                                                                                  aaCTTACGTCTGGTTATTTCTATCGCTAAGCAATACACCAACCGTGGCTTGCAGTTCCTTGACATGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCAGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCCACCGTAt                                                                                                                                 >  1:88571/1‑251 (MQ=255)                                                                                                                     ttACGTCTGGTTATTTCTATCGCTAAGAAATACACTAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTtc                                                                                                                              >  2:505133/1‑251 (MQ=255)                                                                                                                     ttACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTtc                                                                                                                              >  1:454235/1‑251 (MQ=255)                                                                                                                       aCGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTtctc                                                                                                                            <  1:293531/251‑1 (MQ=255)                                                                                                                        cGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTGGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCg                                                                                                                           <  1:376948/251‑1 (MQ=255)                                                                                                                               ttATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGAtg                                                                                                                    >  1:489007/1‑251 (MQ=255)                                                                                                                                   ttCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGATCATTGCCCTGATTCAGGAAGGCAACAT‑CGGTCTGATGCAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTACACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGAtgctgc                                                                                                                >  1:585984/1‑251 (MQ=255)                                                                                                                                    tCTATCGCTAAGAAATACACCAACCGTGCCTTGCAGTTCATTGACCTGATTCAGGAAGGCAACAT‑CGGTATGATGCAAGCGGTTGATAAATTCGAATACCGCCGTGGCTACAAGCCCTCCACCTACGCACCCTGGTGGATCCGTCAGGCGATCCCCCCCTCTATCGCGGCTCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTCTTTCTCGCCCGAtgctgt                                                                                                                >  1:352310/1‑249 (MQ=255)                                                                                                                                       aTCGCTAAGAAATACCCCAACAGTGGCTTGCAGTTCATTGACCTGATTCAGGAAGGCAACAT‑CGGTCTTATGAAAGCGTTTGATAAAGTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATAGGAGTGATACCATCAACAAGCTCAGCCGTATTTGTCGCCAGATGCTGCAaga                                                                                                            <  2:381743/251‑1 (MQ=255)                                                                                                                                          gCTAAGAAATACACCAACCGTAGCTTGCCGTTCCTTGACCTGATTCAGGAAGACAACAT‑CGGTCTGATGAATGCGGTTGATAAATTCGAATACCGCAGTGGTTACAAGCTCGCCACCTACGCACCCTGGTGGATCCGTCAGGTGATCACCCGCTCTATCGCGGATCAGGCGAGCACAATCCGCATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGCATTTCGCGCCAGATGCTGCAagatat                                                                                                         >  2:596486/1‑248 (MQ=255)                                                                                                                                             aaGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGCAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGACGGATCCGTCAGGCGATCAACCACTCTATCGCGGCTAAGGCGCGCACCATCCGTATTCCGGTGCATATTATTAAGACCATCACTAAGATCAAACGTATTTCTCGCCAGATGCTGCAAGAGATggg                                                                                                      >  1:265398/1‑251 (MQ=255)                                                                                                                                             aaGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGCGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGCGATggg                                                                                                      >  2:81970/1‑251 (MQ=255)                                                                                                                                                aaaTACACAAACCGTGGCTTGCAGTTCCTTGCCCTGATTCAGGAAGGCAACAT‑CGGTCTGCTGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTAACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCACGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCACCACGCTCAACCGTATTTCTCGCCAGATGCGGCAAGAGATGGGCCg                                                                                                   >  2:358104/1‑251 (MQ=255)                                                                                                                                                 aaTACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAACGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGt                                                                                                  >  1:62441/1‑251 (MQ=255)                                                                                                                                                          aCCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACAGACg                                                                                         >  1:250110/1‑251 (MQ=255)                                                                                                                                                                gctggCAGGTCCTTGACCTGATACAGAAAGGCAA‑ATACGTTCTGACGAAAGCGTTGGATAAATTCGAAGACCGCCGTGGTTACAAGCTCTCCGCCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCAGATGATTGAGACCATCAACAAGCTCAACCGTAGTTCTCGCCAGATGCTGCAATAGATGGGCTGTGAACCGACGCCGgaa                                                                                   <  2:427102/247‑1 (MQ=255)                                                                                                                                                                    gCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGgaagaa                                                                                <  1:186458/250‑1 (MQ=255)                                                                                                                                                                              gACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATc                                                                                                                                                                                              >  1:212044/1‑130 (MQ=255)                                                                                                                                                                              gACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTGGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATc                                                                                                                                                                                              <  2:212044/130‑1 (MQ=255)                                                                                                                                                                              gACCTGATTCAGGAAGGCAACAG‑CGGTTGGATGAAAGCGGTTGAAAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGGATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAAc                                                                      <  2:251363/250‑1 (MQ=255)                                                                                                                                                                                       cAGGAAGGCAACAT‑CGGTCTAATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGAt                                                            <  2:468322/251‑1 (MQ=255)                                                                                                                                                                                        aGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATg                                                           >  1:382045/1‑251 (MQ=255)                                                                                                                                                                                         ggAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGc                                                          >  1:494577/1‑251 (MQ=255)                                                                                                                                                                                            agtcAACCT‑CGGTCAGATGAAAACGGTTGCTCAACTCGCATACCGCCGTGCTTACCAGCTCTCCACCTACGCAACCTGACGGACCCGCCAGGCGATCCCCCGCTCTACCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCACCAAGCTCAACCGTCTTTCTCGCCAGCTGCGGCAAGAGATGGGCCGTGAACCGACGCCGGAAGACCTGGCTGAACGTATGCCGATGCCgg                                                       >  2:239464/4‑251 (MQ=255)                                                                                                                                                                                             ggCAACAT‑CGGTCTGCTGAAAGCGGTTGATAAATTCTACTACCGCCGTGGCTACAAGCTCTCCACCTACGCAACCTGGTGGCTCCGTAAGGCGATCCCCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTCTTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCgg                                                       >  2:404410/1‑250 (MQ=255)                                                                                                                                                                                               cAACAT‑CGGTCTGATGAAAGCGTTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAg                                                    <  2:32615/251‑1 (MQ=255)                                                                                                                                                                                               cAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGa                                                      >  2:9773/1‑249 (MQ=255)                                                                                                                                                                                                      cGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACa                                                 >  1:371988/1‑248 (MQ=255)                                                                                                                                                                                                       ggccggaggaAAGCGGTTGAAAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGAAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGAt                                             <  2:371988/243‑1 (MQ=255)                                                                                                                                                                                                        gTCTGATGAAAGCGGTTGATAAATTCGAATCCCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACTGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCg                                                                                      <  2:84538/209‑1 (MQ=255)                                                                                                                                                                                                        gTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACTGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCg                                                                                      >  1:84538/1‑209 (MQ=255)                                                                                                                                                                                                             atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCa                                        >  1:454640/1‑250 (MQ=255)                                                                                                                                                                                                               gaAAGCGGTTGATACATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCaa                                       >  1:486366/1‑249 (MQ=255)                                                                                                                                                                                                                aaaGCGGTGGATAAATTCGAACACCGCCGTGGTTACAAGCTCCCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCGGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGt                                    <  2:382045/251‑1 (MQ=255)                                                                                                                                                                                                                 aaggggTTGAAAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCGTCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTg                                   <  2:429865/247‑1 (MQ=255)                                                                                                                                                                                                                  aGCGGGTGATAAATTCGAATACAGCCGTGGTTACAAGCTCACCACCTACGCAACCTGGTGGATCCGTCAGGCGCTCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGc                                  >  2:580842/1‑251 (MQ=255)                                                                                                                                                                                                                   gCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCt                                 <  1:308428/251‑1 (MQ=255)                                                                                                                                                                                                                    cGGTTGATAAATTCGAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACTTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTg                                >  1:594052/1‑251 (MQ=255)                                                                                                                                                                                                                     ggttactaatgtCGAAGACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGTATCCGTCAGGCGATCACCCGCTCTCTCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGAGTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGTCAAGATCCGCAAAGTGCTGa                               <  2:344211/240‑1 (MQ=255)                                                                                                                                                                                                                         gATAAATTCGAATCCCGCCTTGGTTACCAGCTCTCCCCCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCTCAAAGTGCTGAAGAt                           <  1:478786/251‑1 (MQ=255)                                                                                                                                                                                                                                  gAATACCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCaa                     >  1:575146/1‑248 (MQ=255)                                                                                                                                                                                                                                     aacccccgTGGTTACAATCTCTCCACCGACGCAGCCTTGTGGATCCGTCAGGCGATCACCCGCTCGAGCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGAGTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGAGGGGCCGTGAACCGACGCCGGAAGAACGTGCTGAACGTAGGCTGATGCCGGAAGACGAGATGCGCAAATTGCTGAGGATCTCCAAAGAGcc               <  2:252901/246‑1 (MQ=255)                                                                                                                                                                                                                                      aCCGCCGTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCCTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCAGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGcc               >  1:596752/1‑250 (MQ=255)                                                                                                                                                                                                                                            gTGGTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTcc        >  1:422924/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggTTACAAGCTCTCCACCTACGCCACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGc                                                                           >  2:85542/1‑182 (MQ=255)                                                                                                                                                                                                                                              ggTTACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGc                                                                           <  1:85542/182‑1 (MQ=255)                                                                                                                                                                                                                                                ttACAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATgg    >  1:461748/1‑251 (MQ=255)                                                                                                                                                                                                                                                  aCAAGCTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGaa  <  1:81970/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aCAAGCTCTCCACCTACCCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGaa  <  2:454640/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      AGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACAT‑CGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAA  >  NC_000913/3214080‑3214570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑