Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,424,640	T→C	Q198Q (CAA→CAG)	hisM ←	lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,424,640	0	T	C	100.0%	233.4 / NA	65	Q198Q (CAA→CAG)	hisM	lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/35);  total (30/35)

CGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGATTGCCCCAGCAAAAATCTCGGT  >  NC_000913/2424393‑2424882                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   cgaTCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGa                                                                                                                                                                                                                                                 <  2:116454/251‑1 (MQ=255)     ccGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTAGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATACGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACg                                                                                                                                                                                                                                             >  1:293475/1‑251 (MQ=255)     ccGATGATGCATATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATAc                                                                                                                                                                                                                                              <  1:478100/250‑1 (MQ=255)           atgCTTATTACATTTCCGGCATTCGCTTGCAGTGATCCCCCTTTCAGCACTTCCTGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGACGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATCAGAGATGATTAAATAGAGCACCGCGGCAATGCCGACGGCGGTAAAAGGCTGATACGTggcgg                                                                                                                                                                                                                                       >  2:282731/1‑251 (MQ=255)                 attaCATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTa                                                                                                                                                                                                                                  <  2:457001/250‑1 (MQ=255)                 attaCATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTa                                                                                                                                                                                                                                  >  1:264112/1‑250 (MQ=255)                   taCATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAat                                                                                                                                                                                                                                >  2:8990/1‑250 (MQ=255)                            ggCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCt                                                                                                                                                                                                                      <  2:195045/251‑1 (MQ=255)                               aTTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTAtt                                                                                                                                                                                                                   >  1:472369/1‑251 (MQ=255)                                   gCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAGCGATTGTGCACATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTc                                                                                                                                                                                                                >  1:305305/1‑250 (MQ=255)                                    cTTGCAGTGATCCCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACAGCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGCAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTc                                                                                                                                                                                                                <  2:320934/249‑1 (MQ=255)                                              tACCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGc                                                                                                                                                                                                    >  1:123405/1‑251 (MQ=255)                                                ccccGTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTGTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCAc                                                                                                                                                                                                  <  2:512411/251‑1 (MQ=255)                                                 cccTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGATATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCAcc                                                                                                                                                                                                 <  1:286691/251‑1 (MQ=255)                                                 cccTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCAcc                                                                                                                                                                                                 <  1:416448/251‑1 (MQ=255)                                                     tgcAGCACGTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTg                                                                                                                                                                                              <  2:264112/248‑1 (MQ=255)                                                       cAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGcc                                                                                                                                                                                           >  2:363430/1‑250 (MQ=255)                                                       cAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGc                                                                                                                                                                                            >  2:145383/1‑250 (MQ=255)                                                       cAGCACTGCATGTGCGCCGTAGCGTTTGTGCAAATCGATAACTTTTAATTTATTCTCGTACATCGTATTCTCAGTGCGTTGAAGAATGTTTCACATGCTGCAACCAGCGTTTGTCCGCTCTGCGAAAGAGGCTGAGCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATAGCGCTGGCTATTTTCAGCATTTCCGGCAGCGTGGCa                                                                                                                                                                                           <  2:220053/251‑1 (MQ=255)                                                        aGCACTTCATGTTCGCCGTAGCGTTTGTGTAAATCGATAAAGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGTACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAg                                                                                                                                                                                          <  1:321607/251‑1 (MQ=255)                                                              gcATGTTCGCCGTATCGTTTTTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCCCCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGGTAATATCGCGGTCTATTTTCAGCAGATCCTGGACCGTGGCAGTAAAt                                                                                                                                                                                     <  2:360461/249‑1 (MQ=255)                                                                    tCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACCTCGAATTCTCAGTGCGTTGAAGAAGGCTTCCCATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGCCATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACg                                                                                                                                                                              >  1:68473/1‑251 (MQ=255)                                                                                       aaTCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAg                                                                                                                                                           <  2:74844/251‑1 (MQ=255)                                                                                        aTCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAgg                                                                                                                                                          <  2:472369/251‑1 (MQ=255)                                                                                                 gTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATAGCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCg                                                                                                                                                 <  1:96492/251‑1 (MQ=255)                                                                                                             ctcGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGcc                                                                                                                                     >  2:480063/1‑251 (MQ=255)                                                                                                             ctcGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGcc                                                                                                                                     >  2:40069/1‑251 (MQ=255)                                                                                                                    aTCGTATTCTCTGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGCGGCTGATCAGGACATAAGAGATGATTCAATAGAGCACCGCGGCAATGCCGAAGGCGGTACAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCAGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACg                                                                                                                              >  2:261513/1‑251 (MQ=255)                                                                                                                         aTTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAAt                                                                                                                          >  1:325533/1‑250 (MQ=255)                                                                                                                             tcAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATAcgc                                                                                                                      >  1:521106/1‑250 (MQ=255)                                                                                                                              cAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCATGATCACTTCGTTGCTGTATGCCGGTAACGCAATAcg                                                                                                                       <  2:528660/248‑1 (MQ=255)                                                                                                                                 ggCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATGTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCGTCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATAcgcagcgc                                                                                                                 <  2:513043/250‑1 (MQ=255)                                                                                                                                                ttCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAaataat                                                                                                  >  1:123948/1‑251 (MQ=255)                                                                                                                                                  cacaTGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGc                                                                                                <  1:145383/251‑1 (MQ=255)                                                                                                                                                     aTGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGc                                                                                             <  1:480063/251‑1 (MQ=255)                                                                                                                                                     aTGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAAGGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATTCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAAGGCAGc                                                                                             <  1:96289/251‑1 (MQ=255)                                                                                                                                                            aaCCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACAt                                                                                      <  2:361351/251‑1 (MQ=255)                                                                                                                                                                   gTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCTCCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGAGCACGTCGTTGCTGTATGCCGGTAACGGAAAACGCAGCGCAGAAGGCAAAATAATGCAGCGAGACATTTTaaaa                                                                               <  2:238794/251‑1 (MQ=255)                                                                                                                                                                   gTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTaaa                                                                                >  1:163329/1‑250 (MQ=255)                                                                                                                                                                              ctGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAg                                                                      <  1:148554/249‑1 (MQ=255)                                                                                                                                                                                  gAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCAt                                                                  >  1:309787/1‑249 (MQ=255)                                                                                                                                                                                  gAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAg                                                                <  1:80836/251‑1 (MQ=255)                                                                                                                                                                                    aaGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGc                                                              >  1:3340/1‑251 (MQ=255)                                                                                                                                                                                            gATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTggc                                                        <  2:309787/249‑1 (MQ=255)                                                                                                                                                                                             aTCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTggcggc                                                     <  2:451623/251‑1 (MQ=255)                                                                                                                                                                                               cAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCtt                                                   >  1:160916/1‑251 (MQ=255)                                                                                                                                                                                                    cATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAAtt                                              <  1:607600/251‑1 (MQ=255)                                                                                                                                                                                                     aTAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAAttt                                             >  1:217843/1‑251 (MQ=255)                                                                                                                                                                                                           gatgatTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCAGCATGATCACTTCGTTGCTGTATGCCGGTAACGCAATACTCAGCGCAGAAGGCAAAATAATGCAGCTATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCAt                                       <  1:601790/251‑1 (MQ=255)                                                                                                                                                                                                               atgaaaTAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTGCAATTTCCCCATGCgg                                   <  2:494800/248‑1 (MQ=255)                                                                                                                                                                                                                 tAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGa                                  <  1:8990/250‑1 (MQ=255)                                                                                                                                                                                                                   aataggtcACCGCGGCAATTCCGAAGGCGGTAAAAGGCTGATACGTGGCGGAGGTAATAGCGCGGGCTATTTTCAGCAGGTCCGGCACCGGGGCAGTAAATGCCAACGCAGTAGAGTGCAGCAGCAGGATCACTTCGTTGCTGTATTCCGGTAACTCAATACTCAGCGCAGAAGTCAAAATAACGCAGCGAGACAGTTTAAAAGTCTAGAAGCCATATGCTCTGGCGGCGTCAAGCTCTCCAGGCGGAAcc                               <  2:45022/244‑1 (MQ=255)                                                                                                                                                                                                                       gagCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAAc                           >  1:334341/1‑251 (MQ=255)                                                                                                                                                                                                                         gCACCGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACgg                         >  2:342529/1‑251 (MQ=255)                                                                                                                                                                                                                            ccGCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCTCCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACAGTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGAtt                      <  1:32023/251‑1 (MQ=255)                                                                                                                                                                                                                                ggCAATGCCGACGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGATTGccc                  >  1:157877/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaaTGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGATTGCCCCa                <  2:16250/250‑1 (MQ=255)                                                                                                                                                                                                                                   aaTGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTGGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAAGGCAGCGATACATTTGGAAAGTCGAGAAGCGAGATTCTCTGGCGGCTTCAATTTCCCCATGCGTGACCGAACGGATTGCCCCAg               <  2:305305/251‑1 (MQ=255)                                                                                                                                                                                                                                      gCCGAAGGCGGTAAAAGGCTGCTACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCCTGCGGAACCGAACGGATTGCCCCAGCa             >  2:263469/1‑250 (MQ=255)                                                                                                                                                                                                                                       ccGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTCATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCCTCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAACATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGATTGCCCCAGCaa            >  2:255382/1‑250 (MQ=255)                                                                                                                                                                                                                                       ccGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGATTGCCCCAGCaa            >  1:257245/1‑250 (MQ=255)                                                                                                                                                                                                                                       ccGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGATTGCCCCAGCaa            >  2:320029/1‑250 (MQ=255)                                                                                                                                                                                                                                           aGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATACCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGATTGCCCCAGCAAAAAtc       >  1:395957/1‑251 (MQ=255)                                                                                                                                                                                                                                              cGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGATTGCCCCAGCAAAAATCTCg    <  1:320029/251‑1 (MQ=255)                                                                                                                                                                                                                                                 tAAAAGGCTGATACGTGGCGGAGTTAATCTCGCGGGATATTTTCAGCCGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCCGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGATTGCCCCAGCAAAAATCTCGGt  >  2:601373/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CGATCCGATGATGCTTATTACATCTCCGGCATTCGCTTGCAGTGATACCCCTTTCAGCACTTCATGTTCGCCGTAGCGTTTGTGCAAATCGATAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAACGGATTGCCCCAGCAAAAATCTCGGT  >  NC_000913/2424393‑2424882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑