Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,311,650	A→G	F367S (TTC→TCC)	ompC ←	outer membrane porin C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,311,650	0	A	G	100.0%	180.6 / NA	50	F367S (TTC→TCC)	ompC	outer membrane porin C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/24);  total (26/24)

GTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCG  >  NC_000913/2311404‑2311880                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       ggaCCCATAGGTTTGCCTTCAAGAACGGTAACGTCAGTAGCGTGGGTTGTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTg                                                                                                                                                                                                                                    <  2:438836/249‑1 (MQ=255)  tACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAACGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAAAGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCCGGCCCTTTGTTCGATATCAATCGAGATTAGGACTgg                                                                                                                                                                                                                                   >  1:297759/1‑251 (MQ=255)    cccATAGTTTTGCCTTCAAGCACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAAAAAAGAAGCGCGGGGAAGCCAGCAGTGACACGCGGGTAAAGCTTATTTCCCTACGTGATTATCCTCATGCGAACGGTCGCAAGCGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGGCCTTTGTTCGATATCAATCGAGATTAGGACTggg                                                                                                                                                                                                                                  >  2:62922/1‑249 (MQ=255)                   tCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCaga                                                                                                                                                                                                                    <  2:424525/249‑1 (MQ=255)                     aaGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTa                                                                                                                                                                                                                <  2:508183/251‑1 (MQ=255)                       gAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGCTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACg                                                                                                                                                                                                              >  1:290857/1‑251 (MQ=255)                             tAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTAGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAAAAATGAAAAAATGGCCCGCAGGCCCTTTGTTCGATATCAATCGTGGTTAGGACAGGTACACCCGACCCAGAGCTACGAttttt                                                                                                                                                                                                        >  1:423246/1‑247 (MQ=255)                                   cAGTAGCGTGGGCTTTGGCGGGTTGTGGGTTTTGATCGCCAACAACAAAGCCGAGTGCGGAAGCCAGAAGTGCCACCCGGGTAAAGCCTATTTCCATAAGTGCTTATCCTCATGCGAAAGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATAGAGATTAGGAATGGTAAACCAGACCCAGAGCTACGATGTTATTAgtg                                                                                                                                                                                                  >  2:383322/1‑251 (MQ=255)                                        gCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                                                                                                                             <  1:513186/251‑1 (MQ=255)                                        gCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCGACGATGTTATCAGTGTTGAt                                                                                                                                                                                             <  2:290857/251‑1 (MQ=255)                                        gCGTGGGTTTTGGCGGGTTGGGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCATTGCCACGCGGTGAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGGACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCGCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCATTGTTGAt                                                                                                                                                                                             <  2:539760/251‑1 (MQ=255)                                                   ggCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTTGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCAc                                                                                                                                                                                  <  1:74072/251‑1 (MQ=255)                                                    gCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTcccg                                                                                                                                                                                 >  2:483837/1‑248 (MQ=255)                                                      gggTTGTGGTTTTTGATCGCAACCAACAAAGAAGCGCGCGGCAGCCCGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACg                                                                                                                                                                                 >  2:284526/1‑249 (MQ=255)                                                                             caacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAgg                                                                                                                                                        <  1:368202/251‑1 (MQ=255)                                                                                 aaaGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTgt                                                                                                                                                    >  1:331899/1‑250 (MQ=255)                                                                                      agagCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGCTGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTg                                                                                                                                               >  2:53616/1‑251 (MQ=255)                                                                                                agccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                                                                                                     <  2:295621/251‑1 (MQ=255)                                                                                                  ccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                                                                                                                   <  1:310733/251‑1 (MQ=255)                                                                                                            cACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                                                                                                                         <  1:165975/251‑1 (MQ=255)                                                                                                            cACGCGGGTAAAGCTTATTTCCATACGGTATTATCCGCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTTGCGGCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                                                                                                                         <  1:403974/251‑1 (MQ=255)                                                                                                                cgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                                                                                                                     <  1:299070/251‑1 (MQ=255)                                                                                                                  ggTAAAGCTTATTTCCATCCGTGATTATCCTCCTGCGAACGGTCGCAAGAGTACACCAAACAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGACCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                                                                                                   >  2:579208/1‑251 (MQ=255)                                                                                                                     aaaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGGTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGGAGTCAACGTAGGTGGACATGTTTttgttg                                                                                                                 <  2:297759/250‑1 (MQ=255)                                                                                                                                         aTTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaac                                                                                             >  2:416316/1‑250 (MQ=255)                                                                                                                                             tCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGAGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca                                                                                         >  2:425628/1‑250 (MQ=255)                                                                                                                                                     cGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc                                                                                <  2:207850/251‑1 (MQ=255)                                                                                                                                                      gAACGGTCGCAAGAGTACACCAAAAACCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc                                                                                >  2:119600/1‑250 (MQ=255)                                                                                                                                                      gAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc                                                                                >  1:539302/1‑250 (MQ=255)                                                                                                                                                      gAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa                                                                               <  1:62922/251‑1 (MQ=255)                                                                                                                                                               cAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGAAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCtt                                                                      >  1:23386/1‑251 (MQ=255)                                                                                                                                                                aaGAGTCCACCAAAACACTGAGTTTGTACGCTGACAACAATGCAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGCTGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtc                                                                     >  2:254039/1‑251 (MQ=255)                                                                                                                                                                  gagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAACGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt                                                                                  >  2:404959/1‑236 (MQ=255)                                                                                                                                                                  gagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt                                                                                  <  1:404959/236‑1 (MQ=255)                                                                                                                                                                   agTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAGTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGTTAGCACCAACATCAACATATTTCAGGATATCTtcgtc                                                                  <  1:595880/251‑1 (MQ=255)                                                                                                                                                                    gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg                                                                 <  2:200253/251‑1 (MQ=255)                                                                                                                                                                                aCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCCAGCCCTTTGTTCGATATCAATCGAGATTCGGACTGGTAAACCAGACCCAGCGCTACGATGTTCTCAGTGTTGATGCAAGCGTCCCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTCGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACg                                                      >  1:32700/1‑250 (MQ=39)                                                                                                                                                                                       ttGTACGCTGAAAACAATGAAAAAAGGGCCCGCATGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAgg                                               <  2:456081/250‑1 (MQ=255)                                                                                                                                                                                        tGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTCGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGtt                                             >  1:384014/1‑251 (MQ=255)                                                                                                                                                                                              cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTAcc                                       >  2:462620/1‑251 (MQ=255)                                                                                                                                                                                                gAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTGCAGGATATCTTCGTCGTCGTAGCCACTACCCAGGTTGTTAcc                                       <  2:539302/249‑1 (MQ=255)                                                                                                                                                                                                   aaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCttt                                    >  1:459788/1‑249 (MQ=255)                                                                                                                                                                                                      aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACt                               >  1:69539/1‑251 (MQ=255)                                                                                                                                                                                                                  ggccGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGGATGGGAAACCCGGCCCAAAGCTACGATGTTATCAGTGTTGATGCCAGCGGCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCCCCAACCTCAACATATTTCCGGATATCTTCGGCGTCGTAGCCACGACCCAGGGTTTTACCTTTAGACTGCAGGTAAGCCa                   <  2:326864/249‑1 (MQ=255)                                                                                                                                                                                                                     cGCAGGCCCTTTGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTCGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTACGCCAggg                >  2:23983/1‑251 (MQ=255)                                                                                                                                                                                                                            ccTTTGTTCGATATCAATAGAGATTAGGACTGGTAAACCAGACCCAGAGCTCCGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg         >  2:414482/1‑251 (MQ=255)                                                                                                                                                                                                                              tttGTTCGATATAAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGc        >  1:503714/1‑250 (MQ=255)                                                                                                                                                                                                                                tGTTCGATATCAATCGAGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAg      <  1:463431/250‑1 (MQ=255)                                                                                                                                                                                                                                 gTTCGATATCAATCGGGATTAGGACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAGTGTGTAGTCAACGTAGGTGGACATGCTTTTGTTGTAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATGTTCGTCGGCGTAGCGACGACCCAGGTGTGTACCTGGAGACTGCAGGTAGGCCAGGTACGGACCCAGAc    <  2:313076/251‑1 (MQ=255)                                                                                                                                                                                                                                    cGATATCAATCGAGATTACGACTGGTAAACCCGTCCCAGAGCTACGATGTCATCAGTGTTGATGCCAGCGTAACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCACCGTAGGTGGACATGTTTTTGTTGCAGTCGTAGGTAGCACCAACATCAACATCTTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGCTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg  >  2:413318/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       GTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCG  >  NC_000913/2311404‑2311880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑