Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	709,364	A→G	D344G (GAT→GGT)	chiP →	chitooligosaccharide outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	709,364	0	A	G	100.0%	236.2 / NA	66	D344G (GAT→GGT)	chiP	chitooligosaccharide outer membrane channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/38);  total (28/38)

TAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACG  >  NC_000913/709119‑709608                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     gaGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAAcc                                                                                                                                                                                                                                                 <  2:478096/250‑1 (MQ=255)     ccGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTc                                                                                                                                                                                                                                             >  1:526936/1‑251 (MQ=255)        ttAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGACTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGTGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATCTCTGGTGGGGTAACCGTTCTg                                                                                                                                                                                                                                           >  2:108806/1‑250 (MQ=255)                caGCTCCCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGCCCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACg                                                                                                                                                                                                                                  <  2:587050/251‑1 (MQ=255)                     aCCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGT‑CTGACTTCAACGCCAAcc                                                                                                                                                                                                                            >  2:79290/1‑250 (MQ=255)                         gTTCTACGGTACCGGAGATCAAGTTGACGATCGCAGCGTCAACGACCTTTATGCCGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTCGAGGAATTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCg                                                                                                                                                                                                                         >  2:34270/1‑251 (MQ=255)                             tACGGTACCCGAGATACAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGaaa                                                                                                                                                                                                                      >  2:266110/1‑250 (MQ=255)                                 gTACCGGAGATAAAGCTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCCACCTACGCTTCCTCACACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGCCTTCAACGCCAACGGCGACAAAGCg                                                                                                                                                                                                                 >  2:65518/1‑251 (MQ=255)                                   aCCCGAGATAAAGTTGACGAGCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGt                                                                                                                                                                                                               <  2:444196/251‑1 (MQ=255)                                      ggagttaaggTTGCCGATCGCAGCGTCAACGACCTTTATGACTGCACCGCCTGGCTGCGAGCGTTGACCTGTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCTCCTGGGTTAAGGCTGACGGTCAGCAGGGATAGTTCCTGCAACGTATGTCTCGAACCTGCCCTTCCTCAAGCGGTCGCATTGATATCTGGTGGGGTATCAGGTCTGACGTCAATTCCTACGTCGAAAAAGCGGtctc                                                                                                                                                                                                            <  2:67240/242‑2 (MQ=255)                                      cGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGtctt                                                                                                                                                                                                            <  1:228329/251‑1 (MQ=255)                                          aTAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCTTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCttcttc                                                                                                                                                                                                        <  1:213242/251‑1 (MQ=255)                                                    cGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGt                                                                                                                                                                                              <  1:151249/251‑1 (MQ=255)                                                     gATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGt                                                                                                                                                                                              >  2:590713/1‑250 (MQ=255)                                                           aGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATAGCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCt                                                                                                                                                                                       <  1:371799/251‑1 (MQ=255)                                                                 aacttccTTTATGACGTCACCTCCTGTCTTCAATCGTTGACCTGTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCTGCTGGGTTAAGTCTGACGGTCAGCAGGGATCCTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACTGGCGCCTGGATATCTTGTGTGGTAACCGGGCTGACTTCAACGCCATCGCCGATGAAGCGGTTTGCTTCGGTGCGATGTATGACCTGaaaaa                                                                                                                                                                                 <  1:242714/246‑1 (MQ=255)                                                                          tATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGGCCTGAAAAACTGGAATc                                                                                                                                                                         <  1:122227/250‑1 (MQ=255)                                                                           aTGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGGTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTGCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCtt                                                                                                                                                                       <  2:260926/251‑1 (MQ=255)                                                                             gACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTc                                                                                                                                                                      <  2:521957/250‑1 (MQ=255)                                                                                   aCCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCtt                                                                                                                                                               >  2:170635/1‑251 (MQ=255)                                                                                   aCCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCtt                                                                                                                                                               >  2:452002/1‑251 (MQ=255)                                                                                          ggCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCAt                                                                                                                                                         <  1:325627/250‑1 (MQ=255)                                                                                            cTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGAGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTAAGCAGGGATACTTCCTGCACCGTATGAATCCCACCTACGCTTCCTCCAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCACCGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCgg                                                                                                                                                      >  1:312069/1‑251 (MQ=255)                                                                                                  gCGTTGACCTTTGGTTACCGGGCGGGTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCGGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTc                                                                                                                                                <  1:108806/251‑1 (MQ=255)                                                                                                  gCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTc                                                                                                                                                <  1:428808/251‑1 (MQ=255)                                                                                                   cGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTcc                                                                                                                                               <  2:208738/251‑1 (MQ=255)                                                                                                       gACCTTTGGTTACCGGGCGGCTGACGAAGTGGATTTGCGCATCGACGGCAACTGGGTTAAGGATGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCACCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACg                                                                                                                                           >  1:286034/1‑251 (MQ=255)                                                                                                                  aCCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATg                                                                                                                                 <  2:272680/250‑1 (MQ=255)                                                                                                                    cGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATggg                                                                                                                               >  1:174709/1‑250 (MQ=255)                                                                                                                     gggCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGAt                                                                                                                             >  2:337028/1‑251 (MQ=255)                                                                                                                              aCGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCt                                                                                                                    <  1:513269/251‑1 (MQ=255)                                                                                                                               cGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTg                                                                                                                   >  1:160044/1‑251 (MQ=255)                                                                                                                                gTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGc                                                                                                                  >  2:16709/1‑251 (MQ=255)                                                                                                                                   gTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTTCGCATGGGATGCTAACCCTGCGAc                                                                                                               <  1:506088/251‑1 (MQ=255)                                                                                                                                     ggATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGAcc                                                                                                              <  2:160044/250‑1 (MQ=255)                                                                                                                                     ggATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCt                                                                                                             <  1:266110/251‑1 (MQ=255)                                                                                                                                      gATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTg                                                                                                            >  2:430962/1‑251 (MQ=255)                                                                                                                                          tGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACGCCAACCGACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAAACGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCa                                                                                                         <  2:108675/250‑1 (MQ=255)                                                                                                                                           gcgcCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGc                                                                                                          >  1:26044/1‑248 (MQ=255)                                                                                                                                                 cGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGAGGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATc                                                                                                 <  2:95257/251‑1 (MQ=255)                                                                                                                                                        aCCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCCACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGc                                                                                          >  2:369097/1‑251 (MQ=255)                                                                                                                                                         ccTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCg                                                                                         <  1:172972/251‑1 (MQ=255)                                                                                                                                                             ggTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGtact                                                                                     <  2:120151/251‑1 (MQ=255)                                                                                                                                                                  aGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGAc                                                                                <  2:73884/251‑1 (MQ=255)                                                                                                                                                                         cGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAAcc                                                                         >  2:116263/1‑251 (MQ=255)                                                                                                                                                                         cGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAAcc                                                                         >  2:576913/1‑251 (MQ=255)                                                                                                                                                                             cagcagGGATTCTTCCTGCAACGGATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCCTCGCCATCGTCGCTTCCTACGTGTACGCATGGGATGCTAAACCGGCGACCGGGGAGAGCAATCCGGATGCGTACTACGACAAAAACCGGAc                                                                     <  1:50824/251‑1 (MQ=255)                                                                                                                                                                             cagcagGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAGCCTGCGACCGGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGAc                                                                     <  1:76056/251‑1 (MQ=255)                                                                                                                                                                             cagcagGAATACTTCCTGCAACTTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGT‑CTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACt                                                                    <  1:79290/251‑1 (MQ=255)                                                                                                                                                                              agcagGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCATACTACGACAAAAACCGGACt                                                                    <  1:590713/251‑1 (MQ=255)                                                                                                                                                                                  gggATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTc                                                                                                                                                              >  2:450028/1‑157 (MQ=255)                                                                                                                                                                                  gggATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTc                                                                                                                                                              <  1:450028/157‑1 (MQ=255)                                                                                                                                                                                         ttCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTc                                                         >  2:520219/1‑251 (MQ=255)                                                                                                                                                                                                 aCGTATGACTCCAACCTACGCTTCCTCAAAAGGTCGCCTGGATATCTGGTGGGGGAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATTGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACACAAACCGGACTATTGAAGAGTCTGCCTAc                                                  >  1:347126/1‑250 (MQ=255)                                                                                                                                                                                                   gTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTAt                                                                                                                                                                                            >  2:451621/1‑110 (MQ=255)                                                                                                                                                                                                   gTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTAt                                                                                                                                                                                            <  1:451621/110‑1 (MQ=255)                                                                                                                                                                                                           ccAACCTACGCTTCCTCAAACGGTCGCCTGGATAGCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGAt                                         <  2:231904/249‑1 (MQ=255)                                                                                                                                                                                                              aCCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGt                                    >  1:490636/1‑251 (MQ=255)                                                                                                                                                                                                                  actcTTCCTCAAACGGTTGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCGTCGTCGGTGCGATGTATGACCTGAAAAACTGGAATCGTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAGCCTGCGACCGGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATGGAAGAGTCTGCGTTCAGTCTGGATACGGTCtat                                <  2:504285/248‑1 (MQ=255)                                                                                                                                                                                                                  aCGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCta                                 <  1:301236/250‑1 (MQ=255)                                                                                                                                                                                                                    gCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATAc                              >  1:7783/1‑251 (MQ=255)                                                                                                                                                                                                                       tCCTCAAACCGTCGCCAGGAGAGCTGGTGGGGGAACCGTTCTGACGTCAACGCCAACGGCGAAAAAGCGGTCGTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCTCTTCCTACGTGTACGCATGGGATGCTAAACCGGCGACCAGGCAGAGCAATCCGGATACGTACGACGACAATGACCTGACGATGGAAGAGTCGGCCTTCATCCAAGAGGCGGTCTATACCa                            <  2:300117/250‑1 (MQ=255)                                                                                                                                                                                                                        ccTCAAACGGTCGCCTGGATATCTGGTGGGGTAACCGTGCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCAtt                          <  1:63289/251‑1 (MQ=255)                                                                                                                                                                                                                            aaaCGGTCGAATGGCTATCTGGAGGGGTAACCGTTCTGACATCAACGCCAACGGAGAAAAAGCGGTATTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGAACTACGACAAAAACCGGACTATTGAAGAGTCTGACTAAAGCCTGGATGCGGGCTATACCATACAgg                      >  2:580125/1‑251 (MQ=255)                                                                                                                                                                                                                                ggTCGCCTGGATATCTGGTGGGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACgg                  >  2:4107/1‑251 (MQ=255)                                                                                                                                                                                                                                                ggtggGGTAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACg  <  1:170635/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     TAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGATATCTGGTGGGATAACCGTTCTGACTTCAACGCCAACGGCGAAAAAGCGGTCTTCTTCGGTGCGATGTATGACCTGAAAAACTGGAATCTTCCAGGCTTCGCCATCGGCGCTTCCTACGTTTACGCATGGGATGCTAAACCTGCGACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACG  >  NC_000913/709119‑709608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑