Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	124,078	C→T	D354D (GAC→GAT)	aceE →	pyruvate dehydrogenase E1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	124,078	0	C	T	100.0%	340.3 / NA	100	D354D (GAC→GAT)	aceE	pyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/59);  total (41/59)

GGCAAGATCATCAACGAACTGGAAGGCATCTTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAA  >  NC_000913/123833‑124321                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ggCAAGATCATCAACGAACTGGAAGGCATCTTCGAAGGTGCTGGCTGGAACGAGATCAAAGTGCAGAGGGGTCGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTATACTGATCAAGCTGATGAACCAAACCGTTGACGGCGCCTACCAGGCCTTCAAATAGAAAGAAGGCGCGTACGTTCGTG‑AACACTTCTTCGGTAATTATACTGAAACAGCAGCACTGGTTGCAGACTGGACTGATGAGc                                                                                                                                                                                                                                                 >  1:79351/1‑250 (MQ=255)    aaGATCATCAAAGAACTGGAAAGCATCTTCGACGGTGCTGCCTGGTACGTGATCAAAGTGATGTGGGCTAGACGTTGGGCTGAACCGATGCGTAAGGATTCCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGCGCGTACGTTCGTG‑AACACTTCTTCGGTAAATCTCCTGAAACCGCAGCCCTGGTTGCAGTCTGGACTGATGAGCAgtt                                                                                                                                                                                                                                             >  2:592463/1‑249 (MQ=255)    aaGATCAGCAACGAACTGGAAGGCATCTTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTTCGTTCGTG‑AACACTTCTTCGGTAAATATTCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGAt                                                                                                                                                                                                                                             <  1:366050/251‑1 (MQ=255)     aGATCATCAACGAACTGGAAGGCATCTTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATc                                                                                                                                                                                                                                            <  1:358769/251‑1 (MQ=255)           tcaACGAACTGGAAGGCATCTTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGc                                                                                                                                                                                                                                       >  1:327052/1‑250 (MQ=255)            caACGAACTGGAAGGCATCTTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCAc                                                                                                                                                                                                                                     <  2:446150/251‑1 (MQ=255)                gAACTGGAAGGCATCTTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGa                                                                                                                                                                                                                                  >  1:164972/1‑250 (MQ=255)                     ggAAGGCATCTTCGAAGGTGCTGGCTGGAACGTGATCCAAGTGATGTGGGGAAGCCGTTGGGATGCACTGCTGCGTAAGGCTACCAGCGGTAAACTGATCCCGCTGATGAACGAAACAGCTGACGACGACTACCAGACCTTCAAATCGCAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGCAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGACCAGATCTGGGCACTGAACCg                                                                                                                                                                                                                              >  1:41382/1‑249 (MQ=255)                       aaGGCATCTTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGCACCgtggg                                                                                                                                                                                                                          >  2:23748/1‑250 (MQ=255)                       aaGGCATCTTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCgtggt                                                                                                                                                                                                                          <  2:449550/251‑1 (MQ=255)                          gCATCGTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTggtggt                                                                                                                                                                                                                       <  2:410200/251‑1 (MQ=255)                           cATCTTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTc                                                                                                                                                                                                                      <  2:353223/251‑1 (MQ=255)                                 cGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGAt                                                                                                                                                                                                                 <  2:472566/250‑1 (MQ=255)                                   aaGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTT‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTTGGCACGATCCg                                                                                                                                                                                                              <  2:543007/251‑1 (MQ=255)                                    aGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGGAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTCAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTAGTG‑AACACTTCTTCGGTAAATCTCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGACCCGTGGTGGTCACGATCCg                                                                                                                                                                                                              >  2:81963/1‑250 (MQ=255)                                     ggTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCgaa                                                                                                                                                                                                            <  2:159913/251‑1 (MQ=255)                                     ggTGCTGGCTGGAACGTGATCAAAGGGATGTGGGGTAGCCGGTGGGAGGAAATGCTGCGTAAGGATACAAGCGGTAAACTGATCCAGCTGATGCACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGGGCGTACGTTCGTG‑AACACTGCTTCGGTAACTATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGAACTGAACCGTGGTGGTCActgtccga                                                                                                                                                                                                             >  2:455791/1‑243 (MQ=255)                                           ggcttgAACGGGATCAAAGTGATGGGGGGTAGCCGGTGGGATTAACTGCTGCGTGAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGGTC‑TGTAACACTGCTTCGGTAAATATCCTTAAACCGCAGCACTGGTTGCAGAGTGGACCGATGAGCAGATGTGGGCACGTTACCGTGGTGGTGACGATGTGAAGAAAAt                                                                                                                                                                                                      <  2:269780/251‑1 (MQ=255)                                           ggctggAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGaaa                                                                                                                                                                                                        <  2:299564/249‑1 (MQ=255)                                           ggctggAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTGCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGaaa                                                                                                                                                                                                        <  1:294328/249‑1 (MQ=255)                                            gctggAACGTGATCAAAGTGATGTGTGGTAGCCGTTGGGATTAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGTAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATAGCCTGAAACCGCAGCACTGGTTGCAGACTGGTCTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATc                                                                                                                                                                                                     <  2:352330/251‑1 (MQ=255)                                                 aaCGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGAGCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGc                                                                                                                                                                                                <  2:485135/251‑1 (MQ=255)                                                 aaCGTGAACAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGGAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGc                                                                                                                                                                                                <  2:596949/251‑1 (MQ=255)                                                  aCGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCt                                                                                                                                                                                               >  2:383776/1‑251 (MQ=255)                                                       aTCAAAGTGATGTGGGGTAGCCGTTTGGATGAACTGATTCGTAAGGATACCAGCGGTAAACTGATCCGGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTGAAATCGAAAGTTGGTGCGTTCGTTCGTG‑AACACTTCTTCTGTAAATATCCTTAAACCGCTGCGCTGGTTGCAGACTGGACTGATGAGCATATCGTTGTTCTGAGCCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCAtt                                                                                                                                                                                          <  1:303951/251‑1 (MQ=255)                                                         cAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTc                                                                                                                                                                                         <  1:332195/250‑1 (MQ=255)                                                                        tAGCCGGTGGGCTGTACTGCTGCATAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGAGACTACCAGACCTTCGAATCGAAAGATGGAGAGTACGTTCGTG‑AACACTTCTTCGGTAAATCTCCTGAAACCGCAGCACTGGTTGCAGACTGGAATGATGAGCAGATCTGGGCACTGAACCGTGGAGGTCACGATCCGAAGAAAATCTAGGCTGCGTTCACGAAAGCGCAGGaac                                                                                                                                                                         >  2:539592/1‑250 (MQ=255)                                                                          gCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAAcc                                                                                                                                                                       <  1:494904/251‑1 (MQ=255)                                                                                   aTGAACTGCGGCGTAAGGATACCGGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCaaa                                                                                                                                                              <  2:506803/251‑1 (MQ=255)                                                                                     gAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGc                                                                                                                                                            <  1:437576/251‑1 (MQ=255)                                                                                       aCTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCg                                                                                                                                                           >  1:372858/1‑250 (MQ=255)                                                                                       aCTGCTGCGTAAGGATACCAGCGGAGAACTGATCCAGCTGGTGAACGAAACCGATGACGGCGACTACCAGACCTTCAAATCGAAAGATAGAGCGTACGTTCATG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGCCTGATGAGCAGATCTAGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCg                                                                                                                                                           >  1:263922/1‑250 (MQ=255)                                                                                          gctgcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAATCCGTTGACGGCGACTACCAGACCTCCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACGGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAAGCTACGCTGCATTCAAGAAGGCGCAGGAAACCAAAGGCAAAGCGACAg                                                                                                                                                       <  2:183540/251‑1 (MQ=255)                                                                                          gctgcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGAc                                                                                                                                                         <  1:141357/249‑1 (MQ=255)                                                                                           ctgcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGt                                                                                                                                                      >  1:291165/1‑251 (MQ=255)                                                                                             gcGTAAGGATTCCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTGCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTa                                                                                                                                                     <  2:525652/250‑1 (MQ=255)                                                                                             gcGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTa                                                                                                                                                     >  1:311955/1‑250 (MQ=255)                                                                                                  aGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCtt                                                                                                                                               <  1:196679/251‑1 (MQ=255)                                                                                                  aGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCtt                                                                                                                                               <  1:255367/251‑1 (MQ=255)                                                                                                     aTACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCt                                                                                                                                            <  2:97545/251‑1 (MQ=255)                                                                                                       aCCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCCCTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTc                                                                                                                                           >  1:347682/1‑250 (MQ=255)                                                                                                           gCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCCACTCCCAGACCTTCAAATCGAAAGATGGTGCGCCCGTTCGCG‑AACACTTCTTCGGCAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATAcc                                                                                                                                      >  1:246697/1‑251 (MQ=255)                                                                                                             ggTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATAGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCAt                                                                                                                                    >  2:562146/1‑251 (MQ=255)                                                                                                                     gATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGtt                                                                                                                            <  1:608193/251‑1 (MQ=255)                                                                                                                      aTCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTa                                                                                                                           <  1:88639/251‑1 (MQ=255)                                                                                                                      aTCCAGCTGATGAACCAAAAAGTTGCCGGAGACTAGCAGACATTCCAATCGCAAGATGGTGCGTACGTTCGTG‑AACACTTATTCGATAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGtt                                                                                                                            >  2:593915/1‑250 (MQ=255)                                                                                                                          aGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTc                                                                                                                                                                                                                      >  1:388724/1‑156 (MQ=255)                                                                                                                          aGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTc                                                                                                                                                                                                                      <  2:388724/156‑1 (MQ=255)                                                                                                                          aGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAACATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGACACCGCCGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGCACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGc                                                                                                                       >  1:382205/1‑251 (MQ=255)                                                                                                                              gatgaACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCAt                                                                                                                     <  2:235966/249‑1 (MQ=255)                                                                                                                                 gaACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAGCCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCg                                                                                                                <  1:262155/251‑1 (MQ=255)                                                                                                                                 gaACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCCTGGGCg                                                                                                                >  2:505577/1‑251 (MQ=255)                                                                                                                                  aaCGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGa                                                                                                               <  1:363266/251‑1 (MQ=255)                                                                                                                                     gAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AAGACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGAcgc                                                                                                            <  1:425562/251‑1 (MQ=255)                                                                                                                                     gAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTa                                                                                                                                                     >  2:381805/1‑210 (MQ=255)                                                                                                                                     gAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTa                                                                                                                                                     <  1:381805/210‑1 (MQ=255)                                                                                                                                     aaaaCCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTACGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTTGACTTATGAGCAGATCTGGGCACTGAGCCGTGGTTGTCACGAATCGAAGAAAATCTACGCTGTATTCAAGAAAGCTCAGGTATTGAAAGGTAAAGCGACAGTAATCCTTGCTCATACCTGTAAAGGTTTCGGGAAGGGCGAcgc                                                                                                            <  2:367226/250‑1 (MQ=255)                                                                                                                                           gTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGa                                                                                                      <  1:384362/251‑1 (MQ=255)                                                                                                                                           gTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCGCATACCATTAAAGGTTACGGCATGGGAGACGCGGCTGa                                                                                                      <  1:412689/251‑1 (MQ=255)                                                                                                                                             tGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTTGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACTTCATGGGCGACGCGGCTGaa                                                                                                     <  2:510610/250‑1 (MQ=255)                                                                                                                                             tGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGaa                                                                                                     <  1:562146/250‑1 (MQ=255)                                                                                                                                                  gCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTaaa                                                                                               <  2:2215/251‑1 (MQ=255)                                                                                                                                                      cTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGCAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCACGCAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAACGGTTACGGCATGGGCGACGCGGCTGAGGGTaaaacc                                                                                            >  1:289665/1‑248 (MQ=255)                                                                                                                                                         ccAGACCTTCAAATCGAAAGATGGTGCGTACGTGCGTG‑AACACTTCGTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCAGTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTTATCCTTGCTCATAGCATTAATGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATcg                                                                                        <  1:592463/251‑1 (MQ=255)                                                                                                                                                           aGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATcgcg                                                                                      >  1:118225/1‑251 (MQ=255)                                                                                                                                                                   aaaTCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGt                                                                              <  2:327052/251‑1 (MQ=255)                                                                                                                                                                       cGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCAGGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAg                                                                          <  1:62683/251‑1 (MQ=255)                                                                                                                                                                       cGAAAGATGGTGCGTACGTTCGTG‑AACACTTCGTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCAGGAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAg                                                                          <  2:103310/251‑1 (MQ=255)                                                                                                                                                                           aGATGGAGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGTAACCGCAGCACTGGTTGCAGACTGGACTGATGCGCAGATCTGGGCACTGAACCGTGGAGGTCAAGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACAAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTCCGGCATGGGCGACGCGGCTGAAGGGACAAACATCGCGCACCAGGTTACGaaa                                                                       >  1:562412/1‑250 (MQ=255)                                                                                                                                                                            gATGGTGCGCACGTTCGTG‑AACACTTCCTCCGTAAATATCCTGAAACCGCAGCACCGGTTGCAGACTGGACTGCTGAGCAGATCTGCGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCCTACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTCAAAACATCGCGCACCAGGTTAAGacaa                                                                      >  2:409272/1‑247 (MQ=255)                                                                                                                                                                               ggTGCGTACGTTCGTG‑AACCCTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGa                                                                   >  1:454079/1‑250 (MQ=255)                                                                                                                                                                                   cGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATg                                                              <  1:424150/251‑1 (MQ=255)                                                                                                                                                                                     tACGTTAGTC‑AACACTTCTTCGGGAAATATCCTGATATCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTTCGCTGCATTCAAGAAAGCGCCGGAAACCAAAGGCAAAGAGACAGTAATCCTTGCTCATACCATTCAAGGTTACGGCATGGGCGACGCGGCTGCAGGTGAAAACATCGGGCAACAGGCTAAGAAAATGAACaag                                                              >  2:583894/1‑247 (MQ=255)                                                                                                                                                                                      aCGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGAc                                                           <  2:366553/251‑1 (MQ=255)                                                                                                                                                                                      aCGTGCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCTCCATGTTAAGAAAATGAACATGGAc                                                           <  2:557683/251‑1 (MQ=255)                                                                                                                                                                                           cGTG‑AGCACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTCAGAAAATGAACATGGACGgtgg                                                      >  2:241442/1‑250 (MQ=255)                                                                                                                                                                                              g‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTa                                                                                                                                  <  1:610460/172‑1 (MQ=255)                                                                                                                                                                                              g‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTa                                                                                                                                  >  2:610460/1‑172 (MQ=255)                                                                                                                                                                                              g‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCg                                                   >  1:399497/1‑251 (MQ=255)                                                                                                                                                                                                aaCACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTAGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGt                                                  >  1:575573/1‑251 (MQ=255)                                                                                                                                                                                                   acTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAAGCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGGAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCat                                               <  2:372858/251‑1 (MQ=255)                                                                                                                                                                                                                  atCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTc                                >  1:601008/1‑251 (MQ=255)                                                                                                                                                                                                                    ccTGAAAACGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCa                               >  1:144322/1‑250 (MQ=255)                                                                                                                                                                                                                       gAAACCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGGAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAt                             <  1:131282/249‑1 (MQ=255)                                                                                                                                                                                                                         aaCCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCTGGTGAAGGTAAAAGCATCGCGCACCAGGTGAAGAAAATGAACAGGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGc                         <  2:561467/251‑1 (MQ=255)                                                                                                                                                                                                                          aCCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGcc                        <  2:202587/251‑1 (MQ=255)                                                                                                                                                                                                                          aCCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGcc                        <  1:308338/251‑1 (MQ=255)                                                                                                                                                                                                                          aCCGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGcc                        <  1:241442/251‑1 (MQ=255)                                                                                                                                                                                                                           ccGCAGCACTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg                       <  2:551158/251‑1 (MQ=255)                                                                                                                                                                                                                             gcagcaCTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCggg                     >  2:469142/1‑250 (MQ=255)                                                                                                                                                                                                                                 caCTGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCt                 <  1:244246/251‑1 (MQ=255)                                                                                                                                                                                                                                    tGGTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGAt              >  1:229564/1‑251 (MQ=255)                                                                                                                                                                                                                                     ggTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAATAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTAAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGa                                                                   <  1:74402/197‑1 (MQ=255)                                                                                                                                                                                                                                     ggTTGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGa                                                                   >  2:74402/1‑197 (MQ=255)                                                                                                                                                                                                                                     ggTTGCAGACTGGACTGATGAGCAGAGCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCTCACCAGGTGAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGa               <  1:77714/249‑1 (MQ=255)                                                                                                                                                                                                                                        tGCAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGc            >  1:142596/1‑249 (MQ=255)                                                                                                                                                                                                                                          cAGACTGGACTGATGAGCAGATCTGGGCACTGAACCGTGGTGGTGACGATCCGAAGAAAATCTAAGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCATTGATCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGCAGGTCAAAACATCGCGCACCAGGGTAAGAAAATGAACATGGACGGAGTGCGTCATCACCGCGACCGTTTCCAAGTGCCGGTGGCTGATGc            >  1:586206/1‑247 (MQ=255)                                                                                                                                                                                                                                              ctggactgATGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCg    >  2:298386/1‑251 (MQ=255)                                                                                                                                                                                                                                               tggactgATGAGCAGATCTGAGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGACAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGGTGAAGGTAAAAACATCCCGCACCAGGTTAAGAAAATGAACATGGACGGGGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCACATATCg    >  2:41344/1‑250 (MQ=255)                                                                                                                                                                                                                                                ggactgATGAGCAGATCGGGGCACTGAACCGTGGTGTTCACGATCCGAAGAAAATCTACGCTGCAGTCAAGAAATCGCAGGAAACCAAAGGCAATGCTACAGGAATCCTTGCTCATTCTATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGTTAGAAACATCGCGCTCCAGGGTAATAAAATGGTCATGGACGGTTTGTGTCTTTTCCTCGTCCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaa  <  1:281576/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GGCAAGATCATCAACGAACTGGAAGGCATCTTCGAAGGTGCTGGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGGTAAACTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTG‑AACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAA  >  NC_000913/123833‑124321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑