Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	294.8 / NA	84	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (39/45);  total (39/45)

CTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTG  >  NC_000913/3560207‑3560693                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               cttcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGcgc                                                                                                                                                                                                                                               >  2:11110/1‑249 (MQ=255)       ttACCTTCAACGTGTTCGCAACGCTGAGATTGTTGGAGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGTTATCGCTAAACAGCGTCGAGCC‑ATTGGG‑TATTTG‑CTCC‑GCCACTCTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTCTTTGCCCT‑TGCGATCGTGCAACCGCGGGTTAACCGGA‑CTGGCAGGCAGCACC‑GCACCGTCCTGATGGcgcgggatg                                                                                                                                                                                                                                         >  2:11547/1‑243 (MQ=255)          ccATCACCGTTTTAGCAACTTTGATATTGTGGGTGACAAGGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCGGATATCGAAAAACAGGGTCGGGCC‑GTGGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAgg                                                                                                                                                                                                                                      <  1:409558/251‑1 (MQ=255)               aaCGTGTTAGCCACGTTGCGATTGTTGGTGACAAAGCGCAAATTGCTGTGATTGCGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGCGCC‑ATTGGG‑GATTTG‑CTAC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGACGGCAGCGAC‑GCACCGCCCTGATGGCGCGGGATCAGGTTTTg                                                                                                                                                                                                                                 >  2:533941/1‑251 (MQ=255)               aaCGTGTTAGCAACGTGGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCGGTGCGTGCGCTACCGCTCCCGGCGGGGGGCCGATATCGAAAAACAGCGCCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                                 <  1:200754/251‑1 (MQ=255)                ccggtttgacatcGTTGGGATTGGTGGGGACAATGCGAAAATGTCTGTGATGGAGCAGTGCGTCCGCTACCGCTTGCGGCGTGGTGCGGATGTCGATAAACAGCGTCGACCC‑ATTGGG‑GATATG‑CTCC‑GCCTCTTGGCGGGCGATG‑CGGTCTTTTTCTTCGGTCTGGGTGGGCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAATGCAGCGCC‑GCTCCGCCATGATGGCGCAGGATCAGGTTTTGc                                                                                                                                                                                                                                <  1:106242/239‑1 (MQ=255)                aCGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGAAAATTGCTGCGATTGCGCAGTGCGTGCGCTCCCGCTTCCGGCGTGGTACCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GCTTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGCA‑CTGGAAGGCAGCGCC‑GCACCGCCATGCTGGCGCAGGATCAGGTTTTGc                                                                                                                                                                                                                                >  1:361930/1‑251 (MQ=255)                 cGTGTTAGCAACGTTGCGGTTGTTGGTGCCAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAACCAGCGTCGAGCC‑ATTGGG‑GCTTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGGTCGGGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCCTGATGGCGCAGGATCAGGTTTTGCt                                                                                                                                                                                                                               >  2:70820/1‑251 (MQ=255)                 cGTGTTAGCAACGTTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGcc                                                                                                                                                                                                                               >  2:407487/1‑250 (MQ=255)                    gtTAGCAACGTTGAGATTGTTGTTGACAATGCGAAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGAGCAGGTTTTGCTcc                                                                                                                                                                                                                             <  1:177745/250‑1 (MQ=255)                    gtTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCg                                                                                                                                                                                                                            >  1:338169/1‑251 (MQ=255)                    gtTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCg                                                                                                                                                                                                                            >  1:39003/1‑251 (MQ=255)                       aGCAACGTTGAGATTGTTGGAGACAATGCGCAACTTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑CTTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCGACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGccc                                                                                                                                                                                                                         >  2:139741/1‑250 (MQ=255)                               tGAGATTGTTGGTGACAATGCGAAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTATTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGAGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCCCCGCCATGATGGCGCAGGATAAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                 >  2:418936/1‑251 (MQ=255)                                 agaTTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                 >  2:114620/1‑249 (MQ=255)                                         ggTGAGAATGCGCAACTTGCTGTGATTGTGCAGTGCGTGCGCTACCGCTTCCGGCGAGGAGCCGATATCGATAAACAGCGTCGAGCC‑CTTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑AGATCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGACACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGCGGTCgcggc                                                                                                                                                                                                       >  2:584537/1‑251 (MQ=255)                                          gTGACAATGCGCAAATTGCTGTGATTGATCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGTAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                                                                                                                                      >  1:163678/1‑251 (MQ=255)                                            gACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCCTGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCgcggcgg                                                                                                                                                                                                     >  2:434315/1‑249 (MQ=255)                                               aaTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGCCGTGGTGCCGATATCGATAAAAAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAC‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                                   >  2:245905/1‑249 (MQ=255)                                                aTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                                                                                                                                                                                >  1:462220/1‑251 (MQ=255)                                                  gcgaaaaTTGCTGTGATTGAGCAGTGCGTGCGCTCCCGCTTCCGGGGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑TTTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCt                                                                                                                                                                                              <  1:105712/247‑1 (MQ=255)                                                   cgcAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCTATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                                                                                                                             >  1:531934/1‑251 (MQ=255)                                                          tgtttggATTGAGCAGTGGGTGGGCTCCCTCTCCCGGCGTGGTGCGAATGTCAATAAACAGCGGCGGGCG‑AT‑GGG‑GGTTTG‑GTCCCGCCACTTTGCGGGCGAGG‑CGCTCTTTTTCTGCGGTCTGGGGGGCCT‑TGCGATGGTGCCAGGGCGTGTTAACCGAA‑CGGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTGCGGCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt                                                                                                                                                                                       <  1:354641/244‑1 (MQ=255)                                                          tGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTg                                                                                                                                                                                      >  2:511131/1‑251 (MQ=255)                                                           gttgtgATGGAGCGGTGCGTGCGTTCCCGCTTCCGGCGTGGTGCAGATATCGATAAACAGCGTGGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTg                                                                                                                                                                                      <  1:479896/248‑1 (MQ=255)                                                            gtgtgttggggCAGTGCGGGCCCTACCGCTTCGGGGGGGGTGCTAATACGGATAACCTGCGTCGAGCA‑ATGGGG‑GTTTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGGGGCCT‑TGCGATCGTGCCAGGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                                    <  1:592811/241‑1 (MQ=255)                                                                   tGAGCAGTGCGTGCCTTCCCGCTGCCGGCGTGTTGCATATACCAAAAACCAGCGTCGAGCA‑TTTGGG‑GATTTC‑CTCC‑GCCACTTTGCGGGCGATG‑CCCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGGTTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGaa                                                                                                                                                                              <  1:166630/250‑1 (MQ=255)                                                                   ggAGCAGTGCGTGCGCTCCCGCTCCCGGGGTGGTGCCAATACCGATAAACAGCGTCGAGCC‑TTGGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                                                                                                                                              <  1:17423/249‑1 (MQ=255)                                                                     ggCAGTGCGTGCGCTCCCGCTTCCGGCGTGGTGCCGATATCGATAAACGGCGTCGAGCC‑ATGGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                                                                                                                           <  1:114620/250‑1 (MQ=255)                                                                        aGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGCTATAGCTAAACAACGTCGAGCC‑ATTGGG‑CCTTTG‑CTCC‑GCCACTTTGAGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGCTCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                                                                                                                                                                        >  2:538236/1‑251 (MQ=255)                                                                           gcgtgcgCTACGGCTTCGGGCGGGGTGCCGATATCGAAAACCAGGGGGGAGCC‑ATGGGG‑GTTTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                                                                                                                                     <  1:11110/251‑1 (MQ=255)                                                                                   tcccgctccgggCGTGGTGCCATTATCGAAAAACAGCGTCGAGCC‑ATGGGG‑GTTTTG‑CCCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc                                                                                                                                                               <  1:466337/239‑1 (MQ=255)                                                                                    cccGCTCCCGGCGTGGTGCCGATATCGATAAACGGCGTCGAGCC‑ATGGGG‑GTTTTG‑CTCC‑GCCACTTTGCGGGCGAGG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                            <  1:248353/250‑1 (MQ=255)                                                                                            cggcggGGTGCCGATACCGAAAAACAGCGT‑GGGCCTGTTGGG‑GATTTG‑CCCC‑GCCACTTGGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                                                                                                                    <  1:546349/251‑1 (MQ=255)                                                                                             ggggtgggtgcGATAACGAAAAACAGGGGCGTGCC‑AGTGGG‑GGTTTG‑GTCCCGCCCCTGTGCGGGGGATG‑CGCTCTTTTCCTTCGGGCTGGGGGGCTT‑TGCGATCGTGCCACGGCGTGTTAACAGAC‑CTGGAAGGCAGCGCC‑GCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGATTAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                                                                                                                    <  2:522125/241‑1 (MQ=255)                                                                                                gtggtgCCGTTATCTATAAACATCGTCGAGCC‑TTTGTG‑TTTTTC‑CCCC‑CCCTCTTTGCGGGCGAGG‑CGCTCTTTTTCTTCGGTCTGGGGGCCCT‑TGCTATCGTGCCACGGCGTGTTACCCGAA‑CTGGAAGGCAGCTCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATa                                                                                                                                                <  1:556560/251‑1 (MQ=255)                                                                                                   gtgCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                                                                                                                                             >  1:100164/1‑251 (MQ=255)                                                                                                        aatcagaaaaaacagggccgcgCC‑AGTGGG‑GTTTTG‑CCCC‑GCCACTTTGCGGGCGGTG‑CGCTTTTTTTCTTCGGTCGGGGTGGCCT‑TGCGATCGGGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct                                                                                                                                        <  2:434368/230‑1 (MQ=255)                                                                                                             gcaataacaggcgccgggc‑ATTGGGTGATTTG‑CCCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGTTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCCCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                                    <  2:147107/233‑1 (MQ=255)                                                                                                             cGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                                    >  2:436680/1‑250 (MQ=255)                                                                                                                tAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGATGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTAGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                                >  1:545886/1‑251 (MQ=255)                                                                                                                     aGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAAGGAGCAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCCGCTGTTTAAACAggtc                                                                                                                           >  2:330928/1‑248 (MQ=255)                                                                                                                        gggcggggccagtgg‑gATTTGGCTCC‑GCCACTGTGCGGGCGATG‑GGCTCTTTTTCTGCGGTCTGGGTGGCCT‑TGCGATCGGGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCG‑TCGCCGCCATGATGGCGCATGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATATTCTGCGGGCTGACGGAGAAATGCTCTAGCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                                                                                                                          <  1:70820/238‑1 (MQ=255)                                                                                                                         ggtcgaccctttggggttggg‑CTCC‑GCCCCTTTGCGGGCGGTG‑CGCTCTTTTTCTTGGGTGTGGGGGGCCT‑TGCGTTCGTGCCACGGCGTGTTAACCGAA‑CTGGAGGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTGTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa                                                                                                                         <  1:352584/231‑1 (MQ=255)                                                                                                                           gtgccatttGGG‑GTTTTG‑CTCC‑GCCACTTGGCGGGCTATG‑CGCTCTTTTTCTCCGGACTGGGTGGCCT‑TGCGATCGTGCCACGGCGTTTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGTTGTCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa                                                                                                                      <  1:102418/244‑1 (MQ=255)                                                                                                                           gAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTCTCTTCGGTCTGGGAGGACT‑TGAGCTCGTGCCACGGCGTGTTAACCGAA‑CTTGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCAGCTCTTCGGGACTGACATAACCCTGCTGTTTAAACAGTTCGatagt                                                                                                                     >  1:366478/1‑249 (MQ=255)                                                                                                                                gcgggggggggtggggggc‑gccgcTTTGCGGGCGGTG‑CGCCCTTTTTCTAGGGGCGGGGGGGCGT‑GGCGACCGCGCCAGGGCGTGTAACCCGAA‑CTGGAAGGCGGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                                                                                                                   <  2:346243/233‑1 (MQ=255)                                                                                                                                     ggggtttttccTCC‑GCCCCTTGGCGGGGGTGG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAC‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                               <  2:467158/240‑1 (MQ=255)                                                                                                                                     ggggtttgg‑CTCC‑GCCACTTTGCGG‑TGGTGACCCTTTTTTTCTGCGTTCGGGGTGGCCT‑TGCGATCGGCCCTCGGCGTGTTAACCGAA‑CGGGAAGCCAGCGCC‑GCACCGCCATGATTGCGCAGTATCAGGTGTTGCTCCGCCAGCTCATGGAGGTCGCGTCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCAGTACTCTCATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt                                                                                                             <  1:67538/243‑1 (MQ=255)                                                                                                                                       ggttttG‑CTCC‑GCCCCTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGa                                                                                                           <  1:490950/248‑1 (MQ=255)                                                                                                                                         gtttggcccc‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCGGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CGGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAc                                                                                                          <  1:302815/243‑1 (MQ=255)                                                                                                                                         aTTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                         >  2:425076/1‑251 (MQ=255)                                                                                                                                            tG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCCGTTCGATAATACCGTTGTGACGttt                                                                                                      >  1:372089/1‑250 (MQ=255)                                                                                                                                             tgcgcc‑GCCACTTTGCGGGCGATG‑CGCTCTGTTTCTTCGGTCGGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CGGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                                       <  1:533941/246‑1 (MQ=255)                                                                                                                                              gcccc‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CGGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg                                                                                                      <  1:403548/247‑1 (MQ=255)                                                                                                                                              gcccc‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGAGCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTTACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt                                                                                                     <  1:245905/248‑1 (MQ=255)                                                                                                                                                       aCTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTgtttgttt                                                                                             >  1:46977/1‑251 (MQ=255)                                                                                                                                                          tggCGGGCGATG‑CGCTCTTTTTCTTCGGTCGGGGGGGCCT‑TGCGATCGTCCCAGGGCGTGTTACCCGAA‑CTGGAAGGCCGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc                                                                                            <  2:462220/247‑1 (MQ=255)                                                                                                                                                          tggCGGGCGATG‑CGCTCTTTTGCTTCGGTCGGGGGGGCCT‑TGCGAGCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCa                                                                                           <  2:271521/248‑1 (MQ=255)                                                                                                                                                           ggCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCCCGGCGTGTTAACCGAA‑CGGGAAGGCGGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAtt                                                                                         <  1:509781/250‑1 (MQ=255)                                                                                                                                                             cGGGCGGTG‑GGCTCTTTTCCGTCGGTCTGGGTGGCTT‑CGGGATCGTGCCCCGACGTCTTAAC‑GAAGCGGGGAGGAGGCGTC‑GCGCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGACGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTta                                                                                       <  1:11547/251‑1 (MQ=255)                                                                                                                                                              gggggATG‑CGCTCTTTTACTCCGGTCTGGGGGGCCT‑TGCGCTCGGGCCCCGGCGTGTGAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGGTGTGTTTGTTTCATTtat                                                                                      <  2:188353/247‑1 (MQ=255)                                                                                                                                                                 cGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCCTGCCACGGCGTGTTAACCGAA‑CTGGACGGCAGCGCA‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATCGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTaa                                                                                    >  2:389461/1‑250 (MQ=255)                                                                                                                                                                    tG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGTGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATcc                                                                                >  2:275116/1‑251 (MQ=255)                                                                                                                                                                          tctTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTACCCGTA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGCTAATACCGGTGTGCCGTTGTGTTTGTTTCATTTATAAATCCCTgg                                                                            >  2:588682/1‑250 (MQ=255)                                                                                                                                                                              ttgcttgggTCTGGGGGGCCT‑TGCGACCGGGCCACGTGGGGTTAACCGAA‑CTGGAAGGGAGCCCC‑GCCCCGCCAGGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAatt                                                                        <  2:321911/243‑1 (MQ=255)                                                                                                                                                                                   tcGGTCTGGGTGGCCT‑TGCGAGCGTGCAACGGCGTGTTACCCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTGGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattatt                                                                     <  1:418936/248‑1 (MQ=255)                                                                                                                                                                                      gtcggggTGGCCTGTGC‑ATCGGGCCCCGGCGTGTTAACCGAC‑GTGGAAGGCAGCCCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACGGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtt                                                               <  2:522517/247‑1 (MQ=255)                                                                                                                                                                                      gTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGCTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtt                                                               >  1:62203/1‑251 (MQ=255)                                                                                                                                                                                        cTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtttt                                                             >  1:195702/1‑251 (MQ=255)                                                                                                                                                                                          gggTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttcg                                                           >  1:131937/1‑251 (MQ=255)                                                                                                                                                                                                 cT‑TGCGATCGTGCCACGGAGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGCACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGAGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg                                                    >  2:548162/1‑251 (MQ=255)                                                                                                                                                                                                          cGTGCCACGGCGGTTTAACCGAA‑CTGGAAGGCAGCGCC‑GCGCCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTGTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTATCCAGTTCGATAATACCGTTGTGATGGTGTGTTTTGTTGATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATc                                            <  1:390870/251‑1 (MQ=255)                                                                                                                                                                                                              ccACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                            <  2:135177/135‑1 (MQ=255)                                                                                                                                                                                                              ccACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                            >  1:135177/1‑135 (MQ=255)                                                                                                                                                                                                                  ggcgggtaaaCCGAA‑CGGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg                                    <  2:440617/243‑1 (MQ=255)                                                                                                                                                                                                                          aacccgaccgggaagcctgcgcC‑GAACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCTGGCTGACTGAGAAATGCTCTACCAGCTCTTCGGTACTGACATATCCCTGCTGTTTAACCATTTCTATAATACCGTGGTGACTTTGTGTTGGTTTCAGTTATAAATCCCTGGGATTATTTGCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGc                             <  1:268747/233‑1 (MQ=255)                                                                                                                                                                                                                          aaCCGAC‑CGGGAAGGCA‑CGCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTTGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                            <  1:43879/251‑1 (MQ=255)                                                                                                                                                                                                                          aaCCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCCGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                            >  2:421671/1‑251 (MQ=255)                                                                                                                                                                                                                           aCCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                            >  1:404741/1‑250 (MQ=255)                                                                                                                                                                                                                              gAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAc                        >  2:26088/1‑251 (MQ=255)                                                                                                                                                                                                                              gAA‑CTGGAAGCCAGCGCC‑GCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAc                        <  1:286042/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggAAGGCAGCGTC‑GCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAc                    <  1:427803/250‑1 (MQ=255)                                                                                                                                                                                                                                              gcc‑gcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCAAGCCCATTGAGGTCGCGGCGAATAGCCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCgg         >  1:32584/1‑251 (MQ=255)                                                                                                                                                                                                                                                    acagCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTGCCCGCTCTTCGGGACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAGATCCATGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAAAAAAAGCCATCGCTAAACACACGGCTAACCCGGCGAtg    >  1:34058/4‑251 (MQ=255)                                                                                                                                                                                                                                                      cGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCAGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtg  >  1:399619/1‑251 (MQ=255)                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               CTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCC‑ATTGGG‑GATTTG‑CTCC‑GCCACTTTGCGGGCGATG‑CGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAA‑CTGGAAGGCAGCGCC‑GCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTG  >  NC_000913/3560207‑3560693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑