Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	723,528	(T)8→7	coding (887/2685 nt)	kdpD ←	sensor histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	723,528	0	T	.	100.0%	324.1 / NA	70	coding (887/2685 nt)	kdpD	sensor histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (37/33);  total (37/33)

GTGGTTCATCAAGCGCGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGC  >  NC_000913/723283‑723766                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               gtggtTCATCAAGCGCGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGGTCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGGATTGCCCGACGTTTTTTT‑CCGGt                                                                                                                                                                                                                                           >  2:187456/1‑250 (MQ=255)      tcatcaAGCGCGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCgg                                                                                                                                                                                                                                     >  1:131637/1‑251 (MQ=255)       catcaAGCGCGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGt                                                                                                                                                                                                                                    <  1:414687/251‑1 (MQ=255)       catcaAGCGCGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGGCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGt                                                                                                                                                                                                                                    <  2:422443/251‑1 (MQ=255)           aaGCGCGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTgc                                                                                                                                                                                                                                  >  1:404462/1‑249 (MQ=255)             gcgcGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGCCGTTTTTTT‑CCGGTAAGCGGTgcaggg                                                                                                                                                                                                                              >  2:341617/1‑251 (MQ=255)                cgACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTACGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGAGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGCCGTTTTTTT‑CCGGTAAGCGGTgcagggc                                                                                                                                                                                                                             >  1:582974/1‑249 (MQ=255)                cgACCAGCACCTGATCGAGAGCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTgcagggcag                                                                                                                                                                                                                           <  1:404395/251‑1 (MQ=255)                   ccAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAgggg                                                                                                                                                                                                                        <  1:57205/251‑1 (MQ=255)                              ccgagagagggggCGAGGCGCGCAAGACGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGATTAAGCTTGCCGAGATTATGTTCACGGGCAGAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCTCTGAGGATTTCCCTACGGTGTTTG‑CGGGTAAGCTGTGCAGGGCAGGGGTTTCAACATac                                                                                                                                                                                                             <  2:368066/243‑1 (MQ=255)                                     cGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGt                                                                                                                                                                                                      >  1:289087/1‑251 (MQ=255)                                      gggggCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTg                                                                                                                                                                                                     >  1:54042/1‑251 (MQ=255)                                                            tCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGAc                                                                                                                                                                               >  1:322436/1‑251 (MQ=255)                                                                 aaaCGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGcccg                                                                                                                                                                          >  2:502611/1‑249 (MQ=255)                                                                  aaCGTTGCCCGGCGCCCCCAGCCGCGCGAGGCCGGGCGACCGAGAATAATCTTGCGTATATTATGTTCACGGGCATAACTCACTACCTCTTCCTCTTCCGCTGGATCAGAAAGTGTGGCCGTCACCGCGCCCAGCGCCTGCGCCAGACGTAAGGCTCTGAGAAGTGCCCGACGTTGTTTT‑CCGGTAAGCGGGGCATGGCAGGGCTTTCAACATACACCGCGTCCCAGCCGCAACCCAGCCGTGACTCCAgc                                                                                                                                                                         <  2:382849/251‑1 (MQ=255)                                                                     gTTTCCCGACGCCACCAGCGTCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACTCTACCCAGCCGTGACGCCAgccgc                                                                                                                                                                      <  1:516595/251‑1 (MQ=255)                                                                     gTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGCCGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGCCGCTACCCAGCCGTGAGGCCAgccgc                                                                                                                                                                      >  2:23398/1‑251 (MQ=255)                                                                         cccGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgcgcc                                                                                                                                                                   <  1:159666/250‑1 (MQ=255)                                                                          ccGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGTATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCTCTGAGAATTGCCCGACGTGTTTTT‑CCGGTAAGCTGTGCAGGGCAGGGGTTTCAACGTACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgcgccgc                                                                                                                                                                 <  1:608995/251‑1 (MQ=255)                                                                                    caGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTTCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGAcc                                                                                                                                                       >  1:91769/1‑251 (MQ=255)                                                                                     aGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGAcc                                                                                                                                                       >  2:145010/1‑250 (MQ=255)                                                                                        ggcgCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGCCCAGtt                                                                                                                                                   >  2:501527/1‑251 (MQ=255)                                                                                          ggcgAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAGCATACACCGCGTGCCAGACGCTTCCCGGCCGTTACGCCAGCCGCGCCGCTGCTCGGACCAGtttt                                                                                                                                                 <  2:276748/250‑1 (MQ=255)                                                                                           gcgAGGCCGGGCGACCGAGACTAATCTTGCCGCGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGttttt                                                                                                                                                >  2:516593/1‑251 (MQ=255)                                                                                             gAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGCCGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCg                                                                                                                                              >  1:250432/1‑251 (MQ=255)                                                                                                    ggCGACTGAGAATAATCTTGCCGCGATTATGTTCACGGGCATAACGCACTCACGCTTTCTCTTCCGCTGGATCAGCAAGTGTAGCCGTCTCCGCGCCCAGTTCCTGCACCAGAAGTAAGGCGCTGAGAACTGCCCGACGTTTTTTT‑CCTGCAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGAGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCCGCCgg                                                                                                                                       >  2:417006/1‑251 (MQ=255)                                                                                                      cGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGgtg                                                                                                                                     <  2:168638/251‑1 (MQ=255)                                                                                                       gACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGgtgt                                                                                                                                    <  1:187456/251‑1 (MQ=255)                                                                                                       gACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGGTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGgtgt                                                                                                                                    <  1:482472/251‑1 (MQ=255)                                                                                                                 aatCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTCCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACCCCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCt                                                                                                                                                                <  2:82429/213‑1 (MQ=255)                                                                                                                      tGCCGAGATTCTGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATAAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGcct                                                                                                                      >  2:568889/1‑248 (MQ=255)                                                                                                                               tATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAACTGTTGCCGTCTCCGCGCCCCGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGAtcg                                                                                                            >  1:311859/1‑251 (MQ=255)                                                                                                                                   ttCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCCTTAAAGGAtcgcgtc                                                                                                        >  2:418088/1‑251 (MQ=255)                                                                                                                                     cACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAACGCGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTATTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGAtcgcgtcgc                                                                                                      >  1:3481/1‑251 (MQ=255)                                                                                                                                       cGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTcgcgc                                                                                                    >  2:404446/1‑251 (MQ=255)                                                                                                                                       cGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTcgcgc                                                                                                    >  1:28362/1‑251 (MQ=255)                                                                                                                                         ggCATAACGCACTACCGCTTTCTCTTCCGCTGGTTCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTACGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCCGCCGGTGTTATGGCCGATGCATACAAGGAtc                                                                                                             >  2:13605/1‑240 (MQ=255)                                                                                                                                         ggCATAAAGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGAtc                                                                                                             <  1:13605/240‑1 (MQ=255)                                                                                                                                            aTAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCCGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAACGGAtcgcgtgggtcggggg                                                                                               >  2:350264/1‑241 (MQ=255)                                                                                                                                               acgaaCTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATGGCCCGACGTTTGTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCTTGACGCCAGCCGCGCCGCTTCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCca                                                                                            <  1:378165/247‑1 (MQ=255)                                                                                                                                               aCGCACTAACGCTTTCTCTTCCGCTGGCTCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCACGCCGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTCCCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCCTAAAAGGATCGCGTcgcgcgggggccc                                                                                            >  2:228064/1‑244 (MQ=255)                                                                                                                                                  cACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCacac                                                                                         <  1:142592/251‑1 (MQ=255)                                                                                                                                                     tACCGCTTTCTCTTCCGCTGGCTCAGAAAGTGTTGCCGTATCCGCGCACAGTTCCTGCGCCAGACGTACGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTCTTTCGCTGCCGGTGTTATGGCCGCTGCATAACAGGCTCGTGTCGCGCGTGTGCCCCACttt                                                                                      >  2:595695/1‑251 (MQ=255)                                                                                                                                                      aCCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTtc                                                                                     >  1:142813/1‑251 (MQ=255)                                                                                                                                                          cTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTACGCGACCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCtt                                                                                 >  1:368836/1‑251 (MQ=255)                                                                                                                                                            ttCTCTGCCGTTTGATCAGAAAGTTGTGCGGTCACCGCGCCCAGTCACTGCGCTGGCCGTAAGTGGCTGAGAATTGCCCGACGTTTTTTC‑CCGCCAAGCGTTGCATGGCAGGGGTTCCAACATTCACCGCGTGCTGTACGCTTCCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGTACCAGCTTTTCGCTGCCGGTTCTATGGCCGCTTCATAAAAGGATCGCGTCGCGCGTGCGCCACTCTTTCGCTTc                                                                                <  2:197467/250‑1 (MQ=255)                                                                                                                                                            tgctctTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTATT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACGCCGCGTGCCTTATGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTTTTATGGCCTATGCAGAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCtt                                                                                 <  1:398700/247‑1 (MQ=255)                                                                                                                                                             tctcTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCg                                                                               <  1:516593/250‑1 (MQ=255)                                                                                                                                                                ctTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGcc                                                                             <  1:53513/249‑1 (MQ=255)                                                                                                                                                                  tCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAgg                                                                          <  2:289087/250‑1 (MQ=255)                                                                                                                                                                    cgcgggATCAGAAAGTGTGGCCGGCGCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTGT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTGTCTCTTCGCCAGGATg                                                                       <  2:288820/247‑1 (MQ=255)                                                                                                                                                                     gCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGGGCCACACTTTCTCTTCGCCAGGATGc                                                                      >  1:324295/1‑251 (MQ=255)                                                                                                                                                                     gCTGGATCAGAAAGTGTGGCCGCGTCCGCCCCCAGTGCCGGCGCCGGTCGTAAGGCGCTGAGAATTGCCCGACGTTTTTTC‑CCGGTAAGCGGTGCAGGGCAGTGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGGGACGCCATCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCTTCGCGCGTGTGCCACACTTTCTCGTCGCCAGGATGc                                                                      <  2:582974/251‑1 (MQ=255)                                                                                                                                                                             aGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTc                                                                                                                                               <  1:537091/170‑1 (MQ=255)                                                                                                                                                                             aGAAAGTGTTAACGTCTACGCGCCCAGTTCCTGAGCCAGACGTAAGGCGATGAGCATTGCCAGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGATACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTc                                                                                                                                               >  2:537091/1‑170 (MQ=255)                                                                                                                                                                              gAAAGTGTGCCCGTCTCCGCGCCAAGTTCCGGGGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGGGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAg                                                             <  2:341882/251‑1 (MQ=255)                                                                                                                                                                                aaGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGgc                                                           >  2:282735/1‑251 (MQ=255)                                                                                                                                                                                      ggCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCAt                                                     <  1:404446/250‑1 (MQ=255)                                                                                                                                                                                          gTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGTTGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGc                                                 >  1:255153/1‑251 (MQ=255)                                                                                                                                                                                              ccGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACCCCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGAGCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCTCCAGGCGCGCATTTGCtca                                              <  1:480148/250‑1 (MQ=255)                                                                                                                                                                                                        gtccggcgcCGGACGTAAGGCGCTGAGAATTCCCCGACGTTTTTTC‑CCGGTAAGCGGGGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACGTTCGCTTCGCCAGGATGCCCCCGCCAGGCGCGCAGTTGCTCATCAACGCGATc                                   <  2:140890/246‑1 (MQ=255)                                                                                                                                                                                                         tCCTGCTCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATc                                   <  2:146625/250‑1 (MQ=255)                                                                                                                                                                                                          ccTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGCTGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCCCCAGGATGCCCCCGCCAGGCGCGCATATGCTCATCAACGCGATCgg                                 >  2:437279/1‑251 (MQ=255)                                                                                                                                                                                                                ccAGACGTAAGGCGCTGATAATTGCCCGCCGTTTTTGT‑CCGGTAGGCGGGGCAGGGCAGGGGTTTCAACATCCACCTCGTGCCAGACGCTACCCAGCCGTGACTCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCGCATCAACGCGATCGGCAGTAc                           <  2:322436/251‑1 (MQ=255)                                                                                                                                                                                                                  aGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGATTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCCGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTAATCAACGCGATCGGCAGTACgg                         >  1:348389/1‑251 (MQ=255)                                                                                                                                                                                                                        aaGGCGCTGAGAATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCCGGCGCGCATTTGCTCATCAACGCGCTCGGCAGTACGGCGCAgg                   >  2:370564/1‑250 (MQ=255)                                                                                                                                                                                                                                gagaATTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCCTCAACGCGATCGGCAGTACGGCGCCGGGCCAGTTc           >  2:491308/1‑251 (MQ=255)                                                                                                                                                                                                                                   aaTTGCCCGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGCTTCCAACATACGCCGCGTGCCAGACGCTACCCAGCCGTCACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCGGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCTCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGTTCGGCAGTACGGCGCATTGCCAGTTcgcg        <  2:54042/251‑1 (MQ=255)                                                                                                                                                                                                                                        cccGACGTTTTTTT‑CCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAggg   <  1:145010/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccGACGTTTTTTT‑CCGGTCAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGCCGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCCgggg  >  2:287232/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               GTGGTTCATCAAGCGCGACCAGCACCTGATCGAGATCGGGGGCGATGCGCGCCAGTCGGTCAGCAAACGTTTCCCGACGCCACCAGCGGCGCGAGGCCGGGCGACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGC  >  NC_000913/723283‑723766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑